1.Research progress on the relationship between FAK and cancer
Chinese Journal of Clinical Oncology 2015;(2):116-119
Focal adhesion kinase (FAK), a type of protein tyrosine kinase in the cytoplasm, is highly expressed in many types of malignant tumors. FAK is named as such because of its close relationship with the cell adhesion function. Recent studies show that FAK is expressed in many tumor cells and has a definite relationship with cancer progress, metastasis, and prognosis. Accordingly, FAK has become one of the new tumor therapeutic targets that will bring new methods for tumor therapy.
2.P38 Signal Cascades in Hyperosmolarity-Induced Mucous Hypersecretion
Journal of Environment and Health 1992;0(04):-
0.05).Conclusion Hypertonic stress can induce the airway epithelial cells to develop a high mucus secretion with the concentration-dependent manner in the level of transcription,P38 signal pathway plays a major role.
3.Preventive effects of calcium antagonist on injury of renal tubular epithelial cells induced by hyperoxaluria
Journal of Shanghai Jiaotong University(Medical Science) 2009;29(12):1474-1476
Objective To investigate the effects of hyperoxaluria on rat renal tubular epithelium intracellular calcium concentration ([Ca~(2+)]i) and cell apoptosis, and explore the protective effects of calcium antagonist-nifedipine. Methods Sixty male SD rats were randomly divided into six groups (n=10). Rats in water-drinking group were treated with deionized water, nifedipine group with deionized water and nifedipine 10 mg·kg~(-1)·d~(-1), calculi-induced group with deionized water containing 1% ethylene glycol, and three calculi-induced+nifedipine-intervening groups with deionized water containing 1% ethylene glycol plus nifedipine 3, 6 and 10 mg·kg~(-1)·d~(-1), respectively. Four weeks later, the 24 h oxaluria concentration was measured, the apoptosis index of renal tubular epithelial cells was detected with TUNEL method, and Ca~(2+) fluorescence intensity of cells of renal proximal tubules was determined by flow cytometry using Fluo-3/AM staining. Results The 24 h oxaluria concentrations in calculi-induced group and calculi-induced+nifedipine-intervening groups were higher than those in water-drinking group and nifedipine group (P<0.01). The apoptosis index and Ca~(2+) fluorescence intensity were significantly higher in calculi-induced group than those in water-drinking group (P<0.01). The Ca~(2+) fluorescence intensity in calculi-induced+nifedipine (3, 6 and 10 mg·kg~(-1)·d~(-1))-intervening groups was 76.7%, 62.7% and 56.4% of calculi-induced group, respectively, with a significant dose-effect relationship (r=0.839, P<0.01). The apoptosis index of renal tubular epithelial cells was significantly correlated with Ca~(2+) fluorescence intensity (r=0.826, P<0.01). Conclusion Hyperoxaluria can increase apoptosis and [Ca~(2+)]i concentration of renal tubular epithelial cells in rats, and nifedipine can effectively protect renal tubular epithelial cells to resist hyperoxaluria.
4.Clinical efficacy and prognosis of nimotuzumab combined with intensity modulated radiotherapy for advanced local nasopharyngeal carcinoma
Chinese Journal of Primary Medicine and Pharmacy 2017;24(11):1727-1731
Objective To observe the clinical efficacy and prognosis of nimotuzumab combined with intensity modulated radiotherapy(IMRT) in the treatment of advanced local nasopharyngeal carcinoma.Methods 82 patients with advanced nasopharyngeal carcinoma were selected, and they were randomly divided into observation group(40 cases) and control group(42 cases) according to the digital table.The observation group was treated with nimotuzumab plus IMRT plus cisplatin, and the control group was treated with IMRT plus cisplatin.After 7 weeks of treatment, the clinical efficacy, adverse reactions and T cell subsets were observed.And the patients were followed up for 1 year, 2 years and 3 years to evaluate the survival rate.Results In the observation group, the total remission rate was 75.00%,which was higher than that in the control group(59.09%),the difference was statistically significant(x2=35.81,P<0.05).The CD+4,CD+8 and CD+4/CD+8 levels in the observation group were similar to those in the control group(tCD+4=0.59,P=0.56;tCD+8=1.70,P=0.01;tCD+4/CD+8=0.13,P=0.89).The incidence rates of Ⅰ degree and Ⅱ degree mucosal reaction and dermatitis in the observation group were 35.00%,40.00%,40.00%,35.00%,respectively,which were higher than 9.09%,9.09%,9.09%,9.09% of the control group,the differences were statistically significant(x2Ⅰmucous membrane=4.18,P=0.04;x2Ⅱmucous membrane=5.52,P=0.02;x2Ⅰdermatitis=5.52,P=0.02;x2Ⅱdermatitis=4.18,P=0.04).The 2-year and 3-year survival rates in the observation group were 65.50%,60.00%,respectively,which were significantly higher than 31.82%,27.27% in the control group, the differences were statistically significant(x2=4.11,P=0.04;x2=4.58,P=0.03).Conclusion Nimotuzumab combined with IMRT in the treatment of advanced nasopharyngeal carcinoma has good short-term clinical efficacy, good long-term efficacy and controllable adverse reactions.It is worthy of clinical application.
5.Design of the automatic control system for leechdom management in hospital
Wenfeng LI ; Guiling WU ; Zhanjiang DONG
Chinese Medical Equipment Journal 2003;0(11):-
This paper introduces the automatic control system of leechdom management in hospital by computer.It can enhance the managerial level of the medicine and reduce the cost,so the managerial level in hospital can be raised through it.
6.Clinical significance of ultrasound-guided systematic biopsy in patients with prostate cancer
Shuqing LI ; Gang WANG ; Wenfeng XU
Chinese Journal of Urology 1994;0(02):-
4 ng/ml and/or with prostate nodule found on DRE or TRUS . Methods 197 patients suspected of prostate carcinoma underwent ultrasound guided sextant biopsy and 2~4 more cores in the nodule. Four patients whose initial biopsy was negative but subsequent PSA levels were continuously abnormal underwent repeated biopsies. Results Of the 107 cases with prostate nodules,in 34(31.8%) of them prostate cancer was detected,while in 90 cases without a prostate nodule, only 11(12.2%) were positive. Stratified analysis on PSA found that the higher the PSA, the higher the positive biopsy rate of prostate carcinoma. In the 4 cases with repeated biopsies, 1 prostate cancer was detected. Conclusions For patients with prostate nodule and/or PSA over 4 ng/ml,systematic biopsy should be taken, with 2 or more cores toward the nodule.In the patients with continuous high PSA levels,if the first biopsy is negative, rebiopsy should be taken.
7.Research on the application of the anesthesia information system in the hospital
Yi ZHU ; Zhengxiong XU ; Wenfeng LI
China Medical Equipment 2014;(1):69-70,71
The anesthetists not only pay attention to the exploitation and application of the information resource but also rely more and more on the convenience caused by the informatization. Based on HIS, LIS, and PACS, the Anesthesia information system has been constructed in order to guarantee the quality and safety of the anesthesia. The Anesthesia information system shares the information with HIS and LIS and manages the medical information such as the surgery and anesthesia closely. The paper introduces the notion of the anesthesia information system and its main function and also the predicted effect during the management of the anesthesia information system. Meanwhile, some problem about the application has been mentioned.
8.Effect of vitamin K3 decreasing the urine oxalate excretion in rats
Shiqing ZHANG ; Guohai SHI ; Wenfeng LI
Chinese Journal of Urology 2006;0(S2):-
Objective To investigate the effect of vitamin K3 decreasing the urine oxalate excretion in rats. Methods A total of 80 male Sprague-Dawley rats (body weight, 200-250g) were randomly divided into 8 groups, ie, control group, only vitamin K3 group, stone forming group, stone forming plus 4.0、3.0、2.0、0.8、0.4mg/d Vit K3 group. Each group is 10 rats respectively. The change of urine oxalate was observed. Results Vitamin K3 can reduce the 24h urine oxalate excretion in stone-forming group rats, but there were no effects in control group rats(P
9.Experimental study of nifedipine inhibitory effect on calcium oxalate crystallization in rats
Shiqing ZHANG ; Wenfeng LI ; Xing GU
Chinese Journal of Urology 2006;0(S2):-
Objective To determine the effect of nifedipine on the formation of nephrolithiasis in rat models and its mechanism of action. Methods A total of 60 male Sprague-Dawley rats (body weight, 200-250g) were randomly divided into 6 groups, ie, control group, only nifedipine group, stone forming group, stone forming plus 3, 6, 10 mg?kg-1?d-1 nifedipine group. Each group is 10 rats respectively. Hyperoxaluria and calcium oxalate crystals were produced in rats by ethylene glycol in drinking water. Four weeks later, all rats were sacrificed and the calcium oxalate crystallization in kidney, the renal free radical level, the renal cell apoptosis indexes and the blood and urine biochemical indexes were detected. Results The renal calcium oxalate crystallization in the rat with different dose nifedipine was less than the stone forming rats 37.0%, 55.6%, 66.7% significantly (P
10.Different doses of vitamin K3 effect the expression of OPN of rats kidney
Guohai SHI ; Wenfeng LI ; Shiqing ZHANG
Chinese Journal of Urology 2006;0(S2):-
Objective To investigate the effect of different doses of Vitamin K3 on the expression of osteopontin in rats kidney. Methods After different dose of Vitamin K3 were injected to different groups of rats feeded with same stone-inducing agent, the level of expression of OPN in renal tissues was observed with immunohistological staining. Results OPN expression located at distal convoluted tubule when the rats feeded without stone-inducing agent, and the OPN expression stain was extended to proximal convoluted tubule when the stone-inducing agent was feeded. Vitamin K3 can decrease the extension of OPN expression induced by the agent and more doses of Vitamin K3, more effects. Conclusions Vitamin K3 can decrease the expression of OPN in the kidney tubule of rats feeded with stone-induced agent.