Aim To construct the cell model targeted on the damage by α-synuclein for screening anti-Parkinson’s Disease (PD)compounds.Methods The cDNA fragment of α-synucle-in gene was obtained by PCR methods and inserted into the re-combinant prokaryotic plasmid by molecular cloning technique. The recombinant plasmid was transformed into Escherichia coli, and subsequently induced to express α-synuclein protein.The recombinant α-synuclein was purified and identified by affinity chromatography,immunoblotting and mass spectrometry.The cells damage by α-synuclein was evaluated through cell viability measured by 3-(4,5-dimethyl-2-thiazolyl )-2,5-diphenyl-2-H-tetrazolium bromide.Results The obtained cDNA fragment ofα-synuclein in accordance with its theoretic molecular weight was cloned into pET30a plasmid and verified by sequencing.The re-combinant plasmid was transformed into bacteria E.Coli.BL21 (DE3)and induced to express α-synuclein by isopropyl β-D-1 -thiogalactopyranoside (IPTG).The expression condition was op-timized according to the culture temperature,the concentration of IPTG and the proliferation state of bacteria.The purified α-synu-clein was proved to be a 1 5.3 ku molecule weight protein,and could be immunoblotted with anti-α-synuclein antibody.The pu-rified α-synuclein could decrease the viability of PC1 2 cells and primary neurons significantly,and its effect was in a concentra-tion-dependent manner.Conclusion We have succeeded in constructing the cell model targeted on the damage by α-synucle-in.

Object To research and choose the best technology c onditions for extracting ursolic acid from Sambucus chinensis Li ndl. Methods According to physicochemical character of ursolic aci d, orthogonal design and tests of extract technology for ursolic acid were ca rried on, and four factors were chosen such as concentration, qu an tity of ethanol, extracting time, concentration of clearing agent, and three lev els of each factor were used for orthogonal design and test. Results That was the best technology condition that ursolic acid was extracted by means of ethyl alcohol (90%) of 7 times as much as raw materials, and it is heat ed 2 times (each 1 h), and the concentration of clearing agent was 3%. Conclusion That is a better technology for industrial producti on because it is advanced and rational, practical and feasible.

Objective To compare the positioning accuracy between personalized polyurethane foam with wing boards and negative pressure vacuum bag in radiotherapy for lung cancer using kilovoltage cone beam computed tomography(CBCT). Methods Thirty-nine patients with lung cancer who received chest radiotherapy in our hospital from 2015 to 2016 were enrolled as subjects. In those patients, 20 were immobilized by negative pressure vacuum bags(VB group)and the others by personalized polyurethane foam with wing boards(PF group).CBCT images were acquired weekly and registered with planning CT images. Setup errors in the left-right, superior-inferior, and anterior-posterior directions, three-dimensional(3D) displacement vector,and setup time were recorded. The margin of the planning target volume(PTV)was calculated using the Van Herk formula(2.5∑+0.7σ). Between-group comparison was made by paired t test. Results The PF group had a significant smaller setup error in the y-direction than the VB group (2.54±1.79 vs.3.19±2.14 mm,P=0.03),while there were no significant differences in setup errors in the x-or z-direction between the two groups(1.80± 1.48 vs. 1.90± 1.41 mm, P=0.46;2.14± 1.75 vs. 2.25± 1.75 mm,P=0.35). There were no significant differences in rotational setup errors in the Rx-,Ry-,or Rz-direction between the two groups(1.15°±0.76°vs. 1.15°±0.85°, P=0.50;0.71°±0.60°vs. 0.72°±0.43°, P=0.45;0.62°±0.54° vs. 0.46°±0.30°,P=0.06). The PTV margins in the x?,y?,and z?directions were expanded by 5.56, 8.57, and 7.02 mm, respectively, in the PF group, and by 5.62, 9.27, and 7.23 mm,respectively,in the VB group. The proportion of patients with 3D displacement vectors larger than 5 mm was 40% in the PF group and 45% in the VB group.Conclusions For patients undergoing radiotherapy for lung cancer,personalized polyurethane foam with wing boards can,to a certain extent,reduce the setup error in the superior-inferior direction and PTV margin expansion.[Key words] Lung neoplasms/radiotherapy; Polyurethane foam; Vacuum bag; Setup errors;Margin

ObjectiveTo explore the potential suitable distribution area and spatial production area of high-quality medicinal materials of Miao medicine Laportea bulbifera. MethodBased on 200 distribution spots of L. bulbifera and 120 ecological factors, the maximum entropy model（MaxEnt） and ArcGIS software were applied to predict the potential suitable areas. The content of rutin, kaempferol-3-O-rutinoside, and quercitrin from 33 batches of L. bulbifera in Guizhou province was determined by high-performance liquid chromatography (HPLC). Combined with the previous content data of total polysaccharides and total flavonoids, the correlation between the main pharmacodynamic components of L. bulbifera and ecological factors was analyzed. Eventually, the quality regionalization of L. bulbifera was exhibited by the collocated Cokriging in ArcGIS. ResultThe optimum distribution areas of L. bulbifera were Yunnan, Guizhou, Sichuan, Hunan, and Hubei provinces in China, and the high and medium suitable areas accounted for 59.48% of the total distribution areas. October precipitation, slope, min temperature of the coldest month, altitude, solar radiation intensity in June, and April precipitation were the main ecological factors affecting the growth and distribution of L. bulbifera. Temperature, radiation intensity, precipitation, and soil composition and properties had great effects on the accumulation of secondary metabolites in L. bulbifera. The results showed that the high-quality areas of L. bulbifera mainly included Leishan county, Kaili city, Qingzhen city, Pingba district, Huishui county, Longli county, Kaiyang county, and Jiangkou county in Guizhou province. ConclusionL. bulbifera is widely distributed in China， but the suitable distribution areas are mainly concentrated in southwest China and part of central China. The planting bases of L. bulbifera can be selected in parts of cities and counties in southeast and central Guizhou province. In this study， the potential suitable growing areas and the optimum planting areas of L. bulbifera in Guizhou were predicted， which could provide scientific references for the cultivation and the site selection of large-scale planting for L. bulbifera.

OBJECTIVE To opti mize the supercritical CO 2 extraction technology of volatile oil from Blumea balsamifera ，and compare the components of the volatile oil from B. balsamifera obtained by supercritical CO 2 extraction and steam distillation. METHODS The volatile oil of B. balsamifera was extracted by supercritical CO 2 extraction. Using extraction rate of volatile oil as index，extraction temperature ，extraction pressure and extraction time as factors ，based on single-factor experiment ，orthogonal experiment was used to optimize the supercritical CO 2 extraction technology. Gas chromatography-mass spectrometry was used to identify the components of volatile oil from B. balsamifera . Peak area normalization was used to calculate the relative contents of each component. Taking the volatile oil obtained by steam distillation as a reference ，the extraction rates ，components and contents of volatile oil by the two methods were compared. RESULTS The optimal supercritical CO 2 extraction technology of volatile oil from B. balsamifera included extraction pressure of 30 MPa，extraction temperature of 50 ℃ and extracting for 50 min. After 3 times of validation tests ，average extraction rate of volatile oil was 4.64％（RSD＝0.54％，n＝3）. Thirty-nine components such as tritriacontane，stigmasterol，squalene were identified in the volatile oil of B. balsamifera obtained by supercritical CO 2 extraction； and 51 components such as triacontane ，ledol，humulene epoxide Ⅰ were identified by steam distillation. The extraction rate of volatile oil from B. balsamifera obtained by 2 methods were 4.64％ and 0.99％. A total of 26 common components were obtained ， such as xanthoxylin ，L-borneol，β-caryophyllene. Except for xanthoxyline （34.829％ by supercritical CO 2 extraction，30.676％ by steam distillation method ）and phytol （2.401％ by supercritical CO 2 extraction，1.273％ by steam distillation ），the relative contents of the components of volatile oil obtained by supercritical CO 2 extraction were lower than those of steam distillation. CONCLUSIONS The optimal supercritical CO 2 extraction technology is stable and feasible ；the components and contents of volatile oil obtained by two methods varies greatly ，and main compounds are aldehydes and ketones ，alkenes，alcohols and other components.