Objective To observe the effect of Sopfrology-Lamaze childbirth breathing training on the delivery quality and negative mood of pregnant women.Methods 218 pregnant women were sampled and randomly divided into study group(A group,n =122) and control group(B group,n =96).A group learned the method and technique of Sopfrology-Lamaze childbirth breathing.From 28th week pregnancy to delivery,the times of training was more than 3 per week,and lasted 20 minutes.The rate of cesarean delivery,rate of severe pain,the time of labor,the amount of bleeding after 2h labor,and the dosage of patient controlled analgesia were observed.The selfrating anxiety scale (SAS) and self-rating depression scale(SDS) were used to assess the mood of prengnat women in 28th week pregnancy,before and after labor.Results There were significant difference in rate of cesarean delivery(A group 7.4％,B group 17.7％,x2 =5.46,P ＜ 0.01),rate of severe pain (A group18.8％,B group 59.4％,x2 =37.9,P ＜ 0.05),amount of bleeding after 2h labor (A group (219.43-± 31.47) ml,B group (287.5 ± 37.83)ml,t =14.50,P ＜ 0.05),and the dosage of patient controlled analgesia (A group(13.25 ± 1.89)ml,B group (19.87 ±2.52)ml,t=21.43,P＜0.05) between A group and B group.The SAS and SDS scores were lower in A group (SAS:before labor(44.3 ±7.6),after labor(42.2 ±4.7) ;SDS:before labor(45.2 ±4.7),after labor(42.2 ± 5.1),P ＜ 0.05) than those in B group (SAS:before labor(48.5 ± 6.6),after labor(47.35 ±5.1) ; SDS:before labor(47.7 ± 5.3),after labor (46.6 ± 6.3),P ＜ 0.05).Conclusion Sopfrology-Lamaze childbirth breathing training can improve the delivery quality and negative moods of pregnant women

OBJECTIVE: To explore the characteristics and clinical application of alginate dressing to provide better biological dressing for clinical wound healing. METHODS: A computer-based online search of CNKI, Medline, Wanfang, Science Direct, and Ei databases was performed for related articles published between January 1960 and October 2009, with the key words "alginate, dressing, clinical application" in Chinese and English. Studies regarding characteristics and clinical application of alginate dressing were included. Repetitive and Meta analysis were excluded. In addition, related books were manually searched. RESULTS: A total of 65 articles were collected, 17 were included, and 48 repetitive or Meta analyses were excluded. Alginate is natural polysaccharide carbohydrate extracted from sea tangle, and alginate dressing is soft non-textile fiber refined from seaweed. It contains 85% natural alginate fiber and 15% sodium tvIose. It can absorb effusion, form gelate, and exchange Na~+/Ca~(2+) with effusion. Alginate dressing has been widely used in bedsore treatment, anal fistula nursing, stoma nursing, and diabetic foot nursing. Compared with traditional dressing, infection rate of alginate dressing is lower due to good impermeability, promotion of regional vessel proliferation and blood supply, as well as moist and slightly acidic environment which benefits neutrophil function enhances disinfection. Moreover, it can be used in wound healing following abdominal region operation and abscess incision drainage.CONCLUSION: Alginate dressing provides appropriate environment for wound growth, retains active materials in diffusion, promotes active material release, benefit necrotic tissue solution and cell proliferation and differentiation, as well as epithelial cell migration. Moreover, it maintains hypoxia state of wound, stimulates newly generated capillary growth, and promotes granulation tissue growth.

[Objective] To compare the efficacy of Chinese traditional medicine,metformin and cyproterone on reducing insulin resistance,improve metabolic status of glucose and lipid and raise ovulation rate in women with polycysitic ovarian syndrome.[Methods] 100 women with PCOS were divided into three groups:40 patients chosen to be treated by Chinese traditional medicine.The rest were randomly divided into two groups which both contain 30 patients.One group is treated by metformin while another one is treated by cyproterone.Total 87 patients finished the treatment of three months.Basal body temperature(BBT),body mass index,total testosterone,fasting serum insulin and blood sugar levels as well as lipid spectrum were measured and compared among all the patients before and after treatment.[Results] No significant difference is found in the baseline characteristics among three groups before treatment.In Chinese traditional medicine group,90%(36/40)patients finished the treatment,and 33%(12/36)patients ovulated.In metformin group,86%(26/30)patients finished the treatment,and 19%(5/26)patients ovulated.In cyproterone group,83%(25/30)patients finished the treatment,and 16%(4/25)patients ovulated.Ovulation rate was significantly higher in Chinese traditional medicine group than those in metformin and cyproterone groups.There was significant difference between Chinese traditional medicine group and cyproterone group(P

OBJECTIVE:To explore the characteristics and clinical application of alginate dressing to provide better biological dressing for clinical wound healing.METHODS:A computer-based online search of CNKI,Medline,Wanfang,Science Direct,and Ei databases was performed for related articles published between January 1960 and October 2009,with the key words "alginate,dressing,clinical application"in Chinese and English.Studies regarding characteristics and clinical application of alginate dressing were included.Repetitive and Meta analysis were excluded.In addition,related books were manually searched.RESULTS:A total of 65 articles were collected,17 were included,and 48 repetitive or Meta analyses were excluded.Alginate is natural polysaccharide carbohydrate extracted from sea tangle,and alginate dressing is soft non-textile fiber refined from seaweed.It contains 85% natural alginate fiber and 15% sodium tvlose.It can absorb effusion,form gelate,and exchange Na+/Ca2+ with effusion.Alginate dressing has been widely used in bedsore treatment,anal fistula nursing,stoma nursing,and diabetic foot nursing.Compared with traditional dressing,infection rate of alginate dressing is lower due to good impermeability,promotion of regional vessel proliferation and blood supply,as well as moist and slightly acidic environment which benefits neutrophil function enhances disinfection.Moreover,it can be used in wound healing following abdominal region operation and abscess incision drainage.CONCLUSION:Alginate dressing provides appropriate environment for wound growth,retains active materials in diffusion,promotes active material release,benefit necrotic tissue solution and cell proliferation and differentiation,as well as epithelial cell migration.Moreover,it maintains hypoxia state of wound,stimulates newly generated capillary growth,and promotes granulation tissue growth.

Aim To study the effect of two racemic ketamine, S(+)-ketamine and R(-)-ketamine on stimulus-induced superoxide generation and intracellular calcium in vitro. Methods The stimulus-induced superoxide generation in human neutrophils was determined by using method of cytochrome C reduction. The intracellular calcium in human neutrophils was measured by chemiluminescence with Fura-2 loading. The phosphorylation of p47phox of NADPH oxidase in neutrophils was detected by Western blotting. Results S(+)-Ket and R(-)-Ket inhibited fMLP-induced superoxide generation in neutrophils in a concentration-dependent manner. In the case of PMA, S(+)-Ket inhibited PMA-induced superoxide generation and elevation of intracellular calcium of neutrophils in a concentration-dependent manner, whereas R(-)-Ket slightly increased PMA-induced superoxide generation and elevation of intracellular calcium of neutrophils. On the other hand S(+)-Ket inhibited the phosphorytion of p47phox of NADPH oxidase subunit,which R(-)-Ket was increased. EGTA can abolished the inhibition of S(+)-Ket on PMA-induced phosphorytion of p47phox.Conclusion S(+)-Ket inhibits the phosphorylation of p47phox of NADPH oxidase subunit and the superoxide generation in human neutrophils via PKC-calcium signal pathway.

Fibroblast growth factor(FGF)-21 is a novel insulin-independent glucose regulator,and can be a potential therapeutics for treatment of type Ⅱ diabetes.Although FGF-21 was discovered recently,the insight into its biology and therapeutic utility is rapidly evolving.A number of key metabolically-linked molecules and pathways have been suggested to be involved in the mechanism of action of FGF-21,which enables us to renew the understanding of the FGF-21.The aim of this review is to report the update research outcomes.

Influenza viruses can infect humans, lead to epidemics within populations, and even cause global pandemics. During the non-pandemic period, there is a continuous threat as avian or swine influenza viruses cross the species barrier to infect humans, resulting in zoonotic influenza infections. For the purpose of pandemic preparation and control, it is crucial to strengthen surveillance, scientific research, and risk assessment of these zoonotic influenza viruses. Here, we focus on the latest zoonotic influenza viruses that have recently garnered significant attention, providing an overview of their latest epidemiological trends and research progress, thereby facilitating scientific risk assessment.

Objective To explore the effect of continuous nursing based on WeChat public platform on the patients with high risk of pressure ulcers. Methods Selecting 165 pairs of high-risk patients and their caregivers from the emergency department of pressure ulcers in the General Hospital of Chinese People′s Armed Police Forces, and they were divided into control group and experimental group by paired random method. There were 83 pairs in control group, and 82 pairs in experimental group. The control group gave oral education according to the traditional model, and adopted the way of telephone follow-up for continuous nursing. While the experimental group let patients and their family members follow the Wechat public account that specially developed on Wechat platform and get continuous nursing in new models to come true networking health education and communicate with patients and their family members by multi-dimensional nurse. After 3 months intervening, compared patients′quality of life, the care behavior of caregivers and acquisition of knowledge of pressure scores, the incidence of pressure ulcers, degree of satisfaction of high-risk patients and caregivers between the two groups. Results The psychological function score, social function score and quality of life score was respectively ( 67.61 ± 1.67), (58.60 ± 1.52), (50.41 ± 2.70) points in experimental group, and (63.21 ± 1.30), (53.23 ± 1.92), (45.83 ± 2.59) points in control group, the comparative difference of the two groups was statistically significant( t=-4.638,-4.930,-2.749, P<0.05). There was no significant difference in the material life and body function score between two groups(P>0.05). The care behavior of caregivers score and pressure sores knowledge score was respectively (50.84 ± 1.92), (27.83 ± 1.48) points in experimental group, and (46.43 ± 2.40), (19.28 ± 1.31) points in control group, the comparative difference of the two groups was statistically significant (t=-3.192,-9.738, P<0.05 or 0.01). The incidence of pressure ulcers was 15.9%(13/82) in experimental group, significantly lower than that in control group (50.6%, 42/83), two groups was statistically significant (χ2=22.413, P<0.01). The satisfying degree and scores of patients or caregivers for follow-up form, nursing service attitude, follow-up content and the total score was respectively (24.83 ± 3.11), (24.10 ± 2.55), (25.03 ± 2.12), (76.23 ± 2.17) points in experimental group, and (18.34 ± 1.41), (17.41 ± 1.34), (19.85 ± 1.92), (63.25 ± 3.03) points in control group, the comparative difference between two groups had statistical significance (t=-7.797--4.061, all P<0.01). Conclusions Utilizing Wechat public platform continuity of care for patients at high risk of pressure ulcers can improve the patients quality of life, reduce the incidence of pressure ulcers, improve the efficient communication of nurses and patients, and enhance the degree of satisfaction of the patients. This method is convenient, shortcut, economic, practical and worthy for clinical use and promotion .

Objective To investigate the effect of splenectomy on tau expression in rat hippocampus.Methods One hundred and five male SD rats aged 6 months weighing 350-400 g were randomly divided into 3 groups: group A control (n = 15); group B anesthesia (n =45) and group C surgery (n =45). The animals were anesthetized, intubated and mechanically ventilated. In group B and C the animals were anesthetized with 1.5% isoflurane for 2 h. In group C splenectomy was performed. The animals were killed on the 1st, 3rd and 7th day after anesthesia and surgery. The hippocampi were immediately removed for determination of IL-1 and TNF-α mRNA and protein expression, expression of total tau, phosphorylated tau at Thr-205 and Ser-396 and activity of glycogen synthase kinase-3 beta (GSK-3β). Results There was no significant difference in the expression of phosphorylated tau at Thr-205 and Ser-396 between control and anesthesia groups. Surgery significantly increased the expression of IL-1β and TNF-α and induced rapid and massive hyperphosphorylation of tau at Thr-205 and Ser-396 epitope in the hippocampus and activation of GSK-3β. Conclusion Surgical trauma induces inflammatory response in hippocampus, activates GSK-3β and increases phosphorylation of tau.

Objective To evaluate the usefulness of DiversiLab system for genotyping of Acinetobacter baumannii.Methods Fifty-eight non-duplicated clinical Acinetobacter baumannii isolated from 15 cities in China in 2005 were typed by rep-PCR-based DiversiLab system.The results were compared with those of PFGE and multilocus sequence typing. Simpson's index of diversity was used to compare the discriminatory power among DiversiLab system, PFGE and MLST.Results Fifty-eight Acinetobacter baumannii isolates were differentiated into 5 clusters and 25 unique types by DiversiLab system. MLST identified 35 distinct sequence types, which fell into one clone complex of CC22 and 35 singletons, while PFGE resolved 5 pulsotypes and 34 unique types.Simpson's diversity indices for DiversiLab system, MLST and PFGE were O.876, O.944 and 0.961, respectively.Conclusions The discriminatory power of DiversiLab system is lower than that of PFGE and MLST.But as a simple, fast and reproducible typing method, it could be used as a first-line typing tool for the analysis of a large number of isolates.