OBJECTIVE:To study the pharmacological effects of saponin H,a new saponin isolated from Polygala tenuifolia Willd,on isolated smooth muscle and herat.METHODS:Isolated rabbit ileum,aorta strip,Langendorff heart,guinea pig trachea,and rat uterus were prepared.Their changes in tension 3 min after administration of H were recorded.RESULTS:19 and 76 μmol.L-1of H showed contracting on above-mentioned smooth muscles,with increasing their tensions by 64% and 167%,30% and 118%,20% and 50%,100% and 118%,respectively.19,38 and 76μmol.L-1 of H showed dose-dependent inhibition on heart,decreased contractile force by 20%,23% and 34%,and reduced the heart rate by 17%,20% and 35%,respectively,but failed to affect the coronary flow.The contracting action of H on uterus was not influnced by diphenhydramine,but partly attenuated by indomethacin.CONCLUSION:H excited the isolated smooth muscles but inhibited the heart.Its action on uterus was partly related to the activation of prostaglandin synthetase.

Objective: To study the effects of enzymolytic extracts of abalone (EEA) on memory of mice, and compared with water extracts of abalone (WEA). Methods: Mice were given EEA or WEA once daily lasting 10 d. Their step-down latency (SDL) and escape latency (EL) in a passive avoidance , and food-hunting time in a maze were determined. Results: EEA 2-8 ml/kg lengthened SDL by 13.7 %-105.3 %, shortened EL by 40.0 %-60.0 % and food-hunting time by 28.3 %-49.4 %, in a dose-dependent manner. EEA reversed ethanol-induced disturbance of memory retrial in a passive avoidance and NaNO2-induced disruption of memory retention in a passive avoidance and maze. Significant action of WEA was not observed until the dose of WEA was increased to 8 ml/kg. Conclusion: EEA improves learning and memory, more effective than WEA.

Objective To investigate the significance of chromoendoscopy with acetic acid and indigo carmine dye in the diagnosis of early gastric neoplasia and precancerous lesion.Methods After conventional electronic endoscopic diagnosis,From 2011 fanuay to 300 patients of suspicious lesions were randomly divided into endoscopic dye group and control group,each was 150 cases.Patients of endoscopic dye group were directly performed biopsy after acetic acid and indigo carmine dye,while in control group were performed biopsy after only indigo carmine dye.Results In the endoscopic dye group,16 early neoplasia (10.7％),15 severe atypical hyperplasia (10.0％),79 moderate atypical hyperplasia or Intestinal metaplasia (52.7％) and 40 chronic gastritis were found.In the control group,5 early neoplasia (3.3％),10 severe atypical hyperplasia (6.7％),42 moderate atypical hyperplasia or Intestinal metaplasia(28.0％) and 93 chronic gastritis were found.Early gastric cancer and precancerous lesion detection rate of endoscopic dye was significantly higher than that of the control group,and the difference was statistically significant (P ＜ 0.001).After samples were stained with acetic acid and indigo carmine dye,the image of the mucosa of early cancer and severe atypical hyperplasia were faded mucosa,and the image of moderate atypical hyperplasia and Intestinal metaplasia was dyed asymmetrical.Chronic gastritis and normal mucosa was showed dyed equality.Conclusion Chromoendoscopy with acetic acid and indigo carmine dye improve the detection rate of early gastric cancer and precancerous lesion,thereby of high application value.