Objective To analyze the perinatal pregnant women vaginal group B streptococcus (GBS)infec-tion and and adverse pregnancy outcomes and provide evidences which were for clinicians to formulate effective pre-vention and treatment measures.Methods From January 2013 to February 2015,the vaginal secretions were sampled from 795 cases of perinatal pregnant women for culture identification of GBS,and the clinical symptoms and adverse pregnancy outcomes were observed.The final results were statistical analyzed.Results In the 795 pregnant women, there were 256 GBS carriers,the carrier rate was 32.2%.There was statistically significance (χ2 =19.095,P <0.01)between less than 30 years old group (28.9%)and greater than or equal to 30 years old group (42.3%).The different incidence rate of clinical symptoms between GBS negative cases and GBS positive cases (18.8%,8.0%) was also had statistically significance(χ2 =39.514,P <0.01).Compared with the GBS negative groups,the different incidence rate of premature rupture of membranes,preterm delivery,intrauterine infection and neonatal infections of GBS also existed statistical significance (χ2 =29.355,10.543,31.797,13.291,all P <0.01 ).Conclusion The carrier rate of perinatal pregnant women GBS was higher in this region,and the elderly were easier to be infected.Peri-natal pregnant women infected with GBS can increase the occurrence of adverse pregnancy outcomes,and should be given clinical intervention.

Objective To study the correlation of carotid artery atherosclerosis (CAS) and helicobacter pylori (Hp) infection in patients with essential hypertension (EH) by observing the carotid intima-media thickness (CAIMT),helicobacter pylori (Hp)-IgG antibody,blood lipid,high sensitive C-reactive protein (hs-CRP),and homocysteine(HCY).Methods To measure CAIMT,blood lipid,serum Hp-IgG antibody,hs-CRP,and HCY were measured by color doppler ultrasound instrument,and Hp-IgG antibody by colloidal gold method.Results A total of 150 EH patients were randomly divided into CAIMT and Hp-IgG-antibody groups,and were taken statistical analysis.Results There were 89 patients with EH CAIMT ＞ 1.0 mm(59.3％) as the intimal thickening,and 67 cases of Hp-IgG antibody(+) (44.7％) for Hp infection.CAIMT thickening (73.1％) in the Hp infection group was significantly higher than that of Hp infection group (48.2％) (P ＜0.05).In the thickened CAIMT group,Hp infection (55.1％) was significantly higher than that of CAIMT non thickening (29.5％) (P ＜0.05).EH patients complicated with CAIMT thickening and Hp infection had significant correlation (r =0.179,t =2.213,P ＜0.05).In the Hp infection group,the levels of serum triglyceride (TG),total cholesterol(TC),low density lipoprotein cholesterol (LDL-C),HCY,and hs-CRP were significantly higher than those of Hp infection group (P ＜ 0.05).Serum high density lipoprotein cholesterol (HDL-C) were significantly lower than those of the Hp infection group (P ＜ 0.05).Conclusions The study proved the correlation of CAS and Hp infection in patients with EH,and promoted the development of CAS probably by influencing the blood lipid metabolism,inflammatory reaction and HCY levels.It would be one of the treatment of EH with CAS by removing or prevention of Hp infection.

Objective To provide evidences which are for clinicians to formulate effective prevention and treatment measures by analyzing the perinatal pregnant women vaginal group B streptococcus (GBS)infection and adverse pregnancy outcomes. Methods From January 2013 to February 2015,the vaginal secretions were sampled from 795 cases of perinatal pregnant women for culture identification of GBS,the final results were statistical analyzed.Results In the 795 pregnant women ca-ses,there were 256 GBS carriers cases,the carrier rate was 32.2%.There was statistically significance (χ2 = 19.095,P <0.01)between less than 30 years old group (28.9%)and greater than or equal to 30 years old group (42.3%).The differ-ent incidence rate of clinical symptoms between GBS negative cases and GBS positive cases (18.8% vs 8.0%)was also had statistically significance (χ2 =39.514,P <0.01).The antimicrobial resistant rates of ten kinds of antibacterial drugs (vanco-mycin,linezolid,penicillin,ampicillin,ceftriaxone,nitrofurantoin,levofloxacin,clindamycin,erythromycin and tetracycline) was 0,0,0.6%,3.1%,6.6%,9.6%,21.9%,23.8%,29.9% and 58.1% respectively.The positive rate of D-bacteriostatic ring experiment was 23.9%.Conclusion The carrier rate of perinatal pregnant women GBS was higher in this region,and the elderly were easier to be infected.Perinatal pregnant women infected with GBS to vancomycin and rina thiazole amine and penicillin and ampicillin and ceftriaxone and with nitrofurantoin because of the sensitive rate is high.

BACKGROUND:At present, although the study of three-dimensional finite element biomechanical analysis of knee joint meniscus has been reported and we have a certain understanding of the biomechanical changes of the meniscus, but the dynamic simulation of the knee meniscus in the same load conditions in the process of biomechanical analysis of the knee meniscus is less reported. OBJECTIVE:To analyze the biomechanical characteristics of the knee joint meniscus under different flexion angle by using analogue simulation of finite element method. METHODS:Based on knee MRI data of the normal adult volunteers, the medicine finite element simulation software Mimics10.01 and reverse engineering software Rapidform XOR3 were utilized to reconstruct three-dimensional finite element model of knee joint meniscus. The advanced finite element analysis software Abaqus6.10-1 was utilized for analogue simulation and for analyzing biomechanical changes during flexion under vertical load of 300 N. RESULTS AND CONCLUSION:(1) While the knee joint flexed at 0°, 30°, 60° and 90°, with the increase of angle, maximum stress point moved from the anterior edge of tibia attachment surface of the medial meniscus posterior angle to the posterior edge of tibia attachment surface of the lateral meniscus anterior angle, and the stress range of lateral meniscus was greater than that of the medial meniscus. (2) The maximal displacement point moved from the midpoint of inner edge of the medial meniscus to the front outer-upper edge of the lateral meniscus at knee flexion of 0°, 30°, 60° and 90°. Moreover, the range of displacement of lateral meniscus was bigger than the medial meniscus. (3) These findings suggest that the meniscus is the major bearing structure in the process of knee flexion. The lateral meniscus injury rate is greater than the medial meniscus in process of exercise, which is associated with large stress and displacement.

Objective To detect the levels of carotid intima-media thickness(CAIMT),serum homocysteine(HCY),malondialdehyde(MDA) and superoxide dismutase(SOD) activity,in order to investigate the correlation between carotid atherosclerosis(CAS) and serum HCY and oxidative stress in essential hypertension(EH) patients.Methods 50 patients with EH and 266 healthy controls were enrolled in this study.The color Doppler ultrasonography was used to detect CAIMT,and circulating enzyme method was used to detect serum HCY,and MDA levels were detected by TBA and SOD activity was detected by colorimetric assay of and the results of the examination were statistically analyzed.Results The incidence rate of CAS in EH patients was 62.8%(167/266),associated with age,disease and related course,and there was no significant difference in gender.In EH concurrent CAS group,the serum HCY level,MDA level and SOD activity were (28.06±6.94)μmol/L,(13.10±2.75)μmol/L and (69.73±6.51)U/mL,respectively,which of the control group were (11.74±3.62)μmol/L,(4.71±1.18)μmol/L and (136.07±10.16)U/mL,respectively,the levels of serum HCY and MDA in EH complicated CAS group were significantly higher than those in the control group(t=7.118,6.326,all P<0.05),and the activity of SOD was significantly decreased.The serum HCY level was positively correlated with MDA level(r=0.493,t=9.207,P<0.05),and negatively correlated with serum SOD activity(r=-0.562,t=11.040,P<0.05).Conclusion EH patients have high levels of HCY,MDA and SOD activity of low levels.HCY level is significantly increased and oxidative stress is increased in CAS patients.Oxidative stress is associated with serum HCY level.

Objective To explore the method of rapid diagnosis preterm premature rupture of membrane (PPROM)and prognosis factors of residual amniotic fluid in mother and infant through monitoring nonphosphorylated insulin -like growth factor binding protein -1 (IGFBP -1)and the residual amniotic fluid index (AFI)of the cervi-covaginal secretions from PPROM women.Methods 96 cases of PPROM were tested IGFBP -1 of cervicovaginal secretions,AFI were tested by abdominal ultrasound through fast chromatography methods,and the cause was ana-lyzed.The pregnancy outcome and neonatal prognosis were compared.Results 92 cases positive of IGFBP -1 were diagnosed with PPROM.The risk factors of PPROMwere mainly reproductive tract infections,abortion,induced labor history and unknown causes (including no evidence of infection,fetal membrane dysplasia unclear or other factors), the twins,multiplets,macrosomia,breech or cephalopelvic disproportion,gestational hypertension disease,uterine fibroids or deformity,malnutrition and polyhydramnios.In the 92 patients with PPROM pregnant women,the duration from premature rupture of membrane to delivery was (105.7 ±13.9)h,cesarean section rate was 51.1%,fever rate of pregnant women was 7.6%,the rate of placental abruption was 4.3%,the rate of fetal distress was14.1%,perinatal infection rate was 21.7%,and neonatal asphyxia rate was 7.6%,the neonatal mortality was 7.6%.To compare the AFI≤40mm group with AFI >40 mm group,the duration from premature rupture of membrane to delivery was short-ened remarkably (t =10.904,P <0.05),the neonatal mortality rate was significantly increased (χ2 =4.359,P <0.05).Conclusion Measurement of IGFBP -1 and AFI in cervicovaginal secretions can rapid diagnosis PPROMand evaluate the results of pregnancy and neonatal prognosis,and can guide clinical improvement of pregnancy safety and reduce neonatal mortality.

BACKGROUND: At present, the meniscus injury is very common, and can lead to serious injury of the knee joint.Meniscus biomechanics has been reported, but no studies have concerned the biomechanical characteristics of the meniscus in knee buckling during gait under different loading conditions.OBJECTIVE: To simulate and analyze the biomechanical characteristics of the knee joint meniscus under different loading conditions using finite element method.METHODS: Based on the knee magnetic resonance imaging data of normal adult volunteers, three-dimensional finite element model of knee joint was reconstructed. The stress, distribution and changes in the meniscus under different flexion angles were analyzed at 300 N, 400 N and 500 N.RESULTS AND CONCLUSION: (1) Under the respectively loading conditions of 500 N, 400 N and 300 N, the maximum stress point all from the place of posterior horn of medial meniscus anterior surface of tibia moved to the posterior margin of the anterior tibial attachment of the lateral meniscus, and the maximum displacement all from the place of midpoint of the inner edge of the medial meniscus moved to front outer edge of the lateral meniscus during normal dynamic buckling process of 0°, 30°, 60° and 90°, and the range of stress and displacement of lateral meniscus was greater than that of medial meniscus; simultaneously, the distribution range of stress and displacement was proportional to the size of the loading and the angle of the buckling. (2) The three-dimensional finite element model of the knee joint meniscus can be used to effectively dynamically simulate the distribution of force and displacement under different loading conditions, and can provide evidence for speculation of the mechanism of the injury of the meniscus in clinic.

Objective Discusstheinfluence of early diagnosis of myocardial damage and heart-type fatty acid binding protein(h-FABP)on patients with severe head injury after rehabilitation.Methods The sorum h-FABP was detected using enzyme-linked immunosorbent one-step quantitative detection,cTnI using solid-phase enzyme-linked immunosorbent assay(ELISA),CK-MB using the method of immunosuppressive.Results The sorum levels of h-FABP,cTnl and CK-MB in severe head injury patients levels were significantly higher than that of healthy control group(P<0.01),the positive serum h-FABP in severe head injury patients Was significantly higher than cTnI,CK-MB or ECG(P<0.01);8 cases died in47 cases of severe head injury patients,the mortalisty rate was 17.02%(8/47),the mortality rate of abnormal sentrum h-FABP,cTnI and CK-MB group was significantly higher than the normal group(P<0. 01);The serum h-FABP in predicting the death of severe head injury patients was high in sensitivity and negative predictive value,but lower in specificity and accuracy.Condusiom The serum h-FABP quantitative assay as a high-sensitive indicators to minor myocardial dsmage can be used an objective indicators forjudging severity ofacute severe head injury,evaluating incidence of accidents.

OBJECTIVE:To establish a method for the main active components in Citrus chacheiensis with different storage time (1-19 years). METHODS:HPLC was conducted to determine the content of hesperidin:the column was Diamonsil C18 with mobile phase of methanol-acetic acid-water(35∶4∶61,V/V/V)at a flow rate of 1.0 ml/min,detection wavelength was 283 nm,col-umn temperature was 25 ℃,and the injection volume was 5 μl;the contents of nobiletin and tangeretin:the column was Diamon-sil C18 with mobile phase of water-methanol(gradient elution)at a flow rate of 1.0 ml/min,detection wavelength was 326 nm,col-umn temperature was 25 ℃,and the injection volume was 10 μl;and the content of synephrine:the column was Diamonsil C18 with mobile phase of methanol-potassium dihydrogen phosphate solution(taking 0.6 g potassium dihydrogen phosphate,1.0 g sodi-um dodecyl sulfate,1 ml glacial acetic acid dissolved to 1 000 ml)(65∶35,V/V)at a flow rate of 1.0 ml/min,detection wavelength was 275 nm,column temperature was 25 ℃,and the injection volume 20 μl. RESULTS:The linear range was 500-4 500 ng for hesperidin(r=0.999 8),38.816-388.16 ng for nobiletin(r=0.999 6),19.936-199.36 ng for tangeretin(r=0.999 5)and 640-2 560 ng for synephrine(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 3%;recoveries were 96.42%-102.75%(RSD=2.54%,n=6),97.42%-99.95%(RSD=2.46%,n=6),99.26%-106.19%(RSD=2.31%,n=6) and 97.47%-99.76%(RSD=1.95%,n=6),respectively. CONCLUSIONS:The method is simple and stable with good reproducibility, and can be used for the contents determination of main active components in C. chacheiensis with different storage time. Pericarpi-um citri“the older the better”may be irrelevant to the change of the contents of the above-mentioned 4 active components,and it is speculated related to the release of volatile oil content to ease dryness.

Objective To discuss clinical characteristics and surgical treatment of patients with pulmo?nary inflammatory myofibroblastic tumor(PIMT). Methods From April 2010 to June 2015 in Beijing Tiantan Hospital Affiliated to Capital Medical University, there were 15 cases patients diagnosed as PIMT and trea?ted. The clinical datas of the patients were analyzed retrospectively. The main clinical manifestations,pathological results,clinical treatment and prognosis of the patients and follow?up outcome were analyzed. Results There were 10 males and 5 females,at the age of 39 to 6 8 years old with the median age of 52. Among the 15 cases of patients with PIMT,1 case was recurrence,14 cases were initial treatment. The treatment reason was due to the abnormal physical examination,or cough and sputum with blood,or chest pain,or chest tightness or fever. Chest CT showed that the mass was round or class round, lobulated, part of them showed the burr shape edge. Immunohistochemistry showed that Vimentin positive and smooth muscle actin positive. All patients accept?ed the open chest or thoracoscope surgery. The main operations concluded partial resection,lobectomy or lobecto?my with lymph node eradication. No perioperative death occured,no complications such as postoperative bleed?ing,bronchial pleural fistula and other complications happened. The average follow?up time was from 8 to 58 months. Follow?up rate was 100. 0%(15/15). There was no tumor recurrence. Conclusion The clinical mani?festations of the pulmonary inflammatory myofibroblastic tumor is complex. The PIMT should be confirmed by pathologic examination,the main treatment is surgical resection with good prognosis and lower recurrence.