To adapt the postnatal environment,a series of transformations occur to neonatal cardiovascular system after birth.Due to the complexity and variability in this period,many physiological changes and mechanisms are not clear yet.The ideal hemodynamic monitoring should provide non-invasive,continuous,real-time and accurate monitoring of cardiac output,level of tissue perfusion,microcirculation and tissue oxygen transportation,and get complex physiological data,which will provide help for treatments of neonates individually.

The tip of the external ear of the rabbit was cut off with one pair of unsterilizedscissors. The newly formed epidermal cells at the site of the wound were examined atdifferent stages during the healing process with ordinary, special and vital staining methods.It was found that in the neighborhood of the growing epidermis, various kinds of connectivetissue elements, including leucocytes, collagenous fibers, macrophages, cartilages, etc., werebroken up into structureless debris of different sizes and forms. These debris graduallytransformed into newly formed naked nuclei, connecting with the previously formedepidermal cells, and these newly formed naked nuclei progressively developed into newlyformed epidermal cells. There was no evidence of mitotic division among the newly formed epidermis butit may be found in the previously formed region. The possibility of the identity of theseconnective tissue debris with the so-called "the living substance" and their transformationinto newly formed cells was discussed.

AIM:To analyze the effect of non-exchange transfusion therapy, including simple phototherapy or phototherapy combined with albumin therapy, on severe jaundice in full-term neonates.METHODS: The full-term neo-nates (n=110) with serum total bilirubin (TBIL) level over 342 μmol/L recewed simple phototherapy or phototherapy combined with albumin therapy.The changes of serum bilirubin levels and neurological signs of these neonates were ob-served.RESULTS:Serum TBIL and indirect bilirubin ( IBIL) levels in the 2 groups of hospitalized cases significantly re-duced after the first day of treatment and at discharged (P<0.01).The reduced degrees of TBIL and IBIL levels in the neonates given phototherapy combined with albumin therapy were higher than those in the neonates given simple photothera-py.All these neonates did not have bilirubin encephalopathy on admission or at discharged.CONCLUSION:Both simple phototherapy and phototherapy combined with albumin therapy treat severe jaundice effectively and prevent acute bilirubin encephalopathy in full-term neonates.

Objective To explore the influence on each dimension of executive function of social drinkers with moderate dose of alcohol intake and different alcohol expectations.Methods 90 university students who were social drinkers allocated randomly by a lottery method to drinking group (n=31),control group (n=30) and placebo group (n=29).The Stroop task,1-Back task and local-global task were employed to measure executive function from three aspects respectively,namely the inhibition,updating and shifting process.Results Drinking group was compared with the Control group in the three dimensions of executive function:inhibition ((61.65 ± 26.31) ms,(42.48 ± 32.94) ms),updating ((557.23 ± 86.24) ms,(515.02 ± 70.24) ms),and shifting process ((718.85±246.03) ms,(553.95± 151.74) ms).The differences were statistically significant (P＜0.05 or P＜0.01).Placebo group was compared with the control group,in the three dimensions of executive function:inhibition ((39.25±29.35) ms,(42.48±32.94) ms),updating ((516.32±73.25) ms,(515.02±70.24) ms),and shifting process ((591.20± 193.07) ms,(553.95± 151.74) ms),all the differences had no statistically significance (P＞ 0.05).In the drinking group,positive expectation was negatively related to each dimension of executive function (-0.427,-0.330,-0.383).The inhibition and the shifting process appeared significant correlation(P＜0.05).Negative expectation was positively related to each dimension of executive function (0.251,0.323,0.377).The shifting process appeared significant correlation (P＜0.05).Regression analysis showed that positive expectation was significantly negative predictions of inhibition and shifting process.Conclusion Executive function of social drinkers is damaged after moderate alcohol intake,and there are differences between executive function damage of different alcohol expectancy drinkers.

Objective To study the prevalence and subtype of hemorrhagic transformation (HT ) among patients with different subtypes of acute cerebral infarction ,and investigate its clinical significance .Methods 977 patients with acute cerebral infarction from January 2008 to December 2011 were divided into groups according to the stroke subtypes :large-artery atherosclerosis(LAA) , cardioembolism(CE) ,small-artery occlusion(SAO) ,undetermined cause(UND) ,and other determined cause(OC) .HT included hemorrhagic infarction (HI) and parenchymal hematoma (PH) .The baseline data were registered and the prevalence and subtype of HT between different groups were compared .Results The rates of hypertension were the highest in LAA ,The rate of diabetes was the highest in SAO ,The rate of atrial fibrillation was the highest in CE .The rates of hyperlipidemia had no significant difference a-mong different subtypes of CI .The HT incidence of LAA ,CE ,SAO ,OC ,UND were 12 .8% ,31 .1% ,6 .6% ,4 .5% ,5 .5% respec-tively ,the difference was statistically significant (χ2 =61 ,P<0 .01) .As to the subtype of HT ,PH was more common in CE group (χ2 =31 ,P<0 .01) .Conclusion Distribution of risk factors ,HT prevalence and classification are different in different subtypes of acute cerebral infarction ,the differences might be related to the distribution of different risk factors .

Oral cancer is a common cancer of head and neck. Regional recurrence and metastasis are the main course of the poor prognosis. But the exact molecular mechanism is still unknown. Studies have showed that microRNA plays an important role in the tumorigenesis, invasion and metastasis of oral cancer. And it is expected to become a valuable diagnostic marker and therapeutic target. This paper makes a review about the microRNA related to oral cancer.

Objective:To observe the effect of Wuling capsules combined with calcitriol on urine marker proteins in early diabetic nephropathy ( DN) . Methods: Totally 64 patients with early DN were randomly divided into the control group and the observation group with 32 cases in each. The two groups were with appropriate diet and exercise, relatively stable protein intake and optimal hypo-glycemic program. The observation group was additionally treated with Wuling capsules (0. 9 g, po tid) and calcitriol 0. 25 μg,po qd, with the treatment course of eight weeks. The levels of FBG and 2hBG, HbA1c, hs-CRP, urinary protein, ALB, β2-MG and RBP were observed in the two groups before and after the treatment. Results:After the treatment, FBG, 2hBG, HbA1c, hs-CRP, total pro-tein, Alb and RBP in the two groups were improved when compared with those before the treatment, and the difference had statistical significance(P<0. 05). The total protein, ALB,β2-MG, RBP and hs-CRP in the observation group were better than those in the con-trol group after the treatment, and the difference was statistically significance (P<0. 05). Conclusion: Wuling capsules combined with calcitriol can effectively reduce glomerular and renal tubular damage in early DN patients.

Objective To investigate the effects of serum brain natriuretic peptide(BNP), procalcitonin (PCT) and lung function by tiotropium bromide combined with aminophylline in the treatment of senile chronic obstructive pulmonary disease.Methods A total of 82 cases of elderly patients with chronic obstructive pulmonary disease ( COPD) were collected and randomly divided into experimental group and control group with 41 cases in each group.Patients in the control group were treated by conventional treatment; patients in the experimental group were treated on the basis of the control group with tiotropium bromide combined with aminophylline.The lung function, respiratory condition and serum levels of PCT, BNP before and after treatment were determined, and the clinical efficacy and safety was compared.Results The effective rate of the control group (68.29%) was lower than the experimental group (87.80%) , with statistical significance ( P<0.05 ); compared with the control group after treatment, the forced expiratory volume in 1 second (FEV1), forced vital capacity (FVC) and FEV1%pre of the experimental group significantly increased, dyspnea score decreased, 4 min walking test increased, serum PCT and BNP levels decreased in the experimental group, with statistical significance ( P<0.05 ). Conclusion Tiotropium bromide combined with aminophylline in the treatment of senile chronic obstructive pulmonary disease could reduce the serum BNP and PCT levels and improve lung function.

Objective To report on our experience with laparoscopic nephron-sparing surgery forthe treatment of renal tumors,and to seek the safe and effective techniques and methods. Methods FromJune 2003 to June 2005,16 patients (5 men and 11 women) with small exophytic solid renal masses weretreated by transperitoneal laparoscopic wedge resection in our hospital.The mean age was 46 years (range,29 -56 years).The mean tumor size of renal cell carcinoma (5 cases) and hamartoma (11 cases) was2.0 -3.5 cm and 3.0 -5.5 cm,respectively, in diameter. One case of hamartoma had secondary bleeding.Wedge resection of the tumors was performed quickly with scissor,and hemostasis was achieved by intra-ab-domen suturing and knotting. Results All the procedures were finished laparoscopically with no conver-sion to open surgery.The mean operative time was 104 min (range,70 -150 min);mean hot bloodless timewas 21 min(range,14 -32 min);mean blood loss was 158 ml (range,50 -700 ml).The pathologic exami-nation showed negative surgical margin in 5 cases of renal cell carcinoma.Postoperatively,no urinary leakageand secondary bleeding occurred,and the renal function was normal in all the 16 cases.The patients weredischarged 7 d after operation.Follow-up was 1 month to 1 year.Neither distant nor local recurrences wereobserved by the last follow-up date on B-ultrasound,IVUand CTat follow-up. Conclusions Laparoscopicnephron-sparing surgery for renal tumors is a minimally invasive procedure with less blood loss,less pain andfewer complications.Reliable non-traumatic kidney vessel control is the basic method of this operation.Sharpresection without smog and rapid renal incision suturing can reduce the renal hot bloodless time.

OBJECTIVE:To study the pharmacodynamics of Jinkecuo capsule. METHODS:Foot swelling mice model and cotton ball granuloma rats model were induced using carrageenin for the observation of the anti-inflammatory effect of Jinkecuo capsule;Propionibacterium-induced rats' auricle acnitis model was duplicated for observation of the effect of the Jinkecuo capsule on the propionibacterium-induced acnitis.RESULTS:Jinkecuo capsule had marked inhibitory effect on carrageenan-induced foot swelling of mice (P