BACKGROUND: Poly-lactic glycolic acid (PLGA) is a promising cell scaffold material. However, its hydrophilicity and cellular affinity is poor, so it is necessary to modify its performance.OBJECTIVE: To explore the hydrophilic ability of the modified PLGA scaffold compounded with type Ⅰ collagen, and the cellular biocompatibility with chondrocyte of rabbit ear.METHODS: PLGA scaffold was modified with Poly-L-Lysine and compounded with type I collagen. The gross structure of scaffold was observed by inverted microscopy. The modified PLGNtype-Ⅰ collagen scaffold (experimental) and PLGA scaffold (control) were immerged in distilled water for 0.5, 1, 2, 4, 8, 12 and 24 hours. Chondrocytes were cultured by enzyme digestion method, and the second passage cells were seeded on surfaces of two scaffolds. Cell morphology was observed by phase contrast microscopy; cell attachment rate 24 hours after seeding was calculated, and the cell proliferation was determined by MTT assay at 1, 2, 4 and 6 days.RESULTS AND CONCLUSION: Modified composite scaffold exhibited high porosity and increased surface roughness compared with control group. Water uptake of two scaffolds displayed statistically significance at the same time point (P < 0.01), indicating the modification improved the hydrophilicity. The attachment rate of chondrocytes was 0.908 0+0.019 2 in modified compound scaffolds and 0.733 2±0.047 5 in control scaffold after 24 hours (P < 0.05), indicating the improved cellular affinity following modification. After 1, 2, 4 and 6 days, the absorbance between two groups was significantly different (P < 0.05), indicating the modified scaffold improved cell proliferation.

BACKGROUND:Knee cartilage injury is difficult to heal, which is an urgent clinical problem to solve. OBJECTIVE:To summarize the advantages of synovial mesenchymal stem cel s in the treatment of knee cartilage injury. METHODS:A computer-based search of PubMed was performed for articles related to synovial mesenchymal stem cel s for knee cartilage injury using the keywords of“knee joint, cartilage, synovial membrane, tissues, injuries and repair”. A total of 48 articles were retrieved initial y, and 35 articles were included in result analysis. RESULTS AND CONCLUSION:Synovial mesenchymal stem cel s for treatment of knee cartilage injury can achieve more ideal outcomes. Mesenchymal stem cel s inherent in the damaged tissue are the optimal seed cel s for tissue repair.

objective: To evaluate the effects of the pendulum appliance on arch length, arch width, overbite and overjet. Methods: Pendulum appliances were applied for distalization of maxillary molars in 37 patients whith class Ⅱ malocclusion and 1-Ⅱ degree crowded dentition in upper dental arch. Pretreatment and posttreatment model analysis was conducted. Results: 37 patients treated for 18.2?4.5 weeks with pendulum appliance were accomplished . The increase of arch arc length between the maxillary first molars was (17.36?4.38) mm, the decrease of anterior arch width (-3.04?1.59) mm, and the decrease of posterior arch width (-1.29?2.01) mm . Differences between the pretreatment and posttreatment were significant, including arch arc length between the maxillary first molars ( P

Objective:To evaluate effects of traction arch combined with protraction on dental and skeletal changes in patients with Angel Ⅲ malocclusion in mixed dentition.Methods:Seventeen patients with Angle Ⅲ malocclusion were selected and treated with traction arch combined with protraction. Cephalometric radiographs were taken 6 months before the initiation of treatment, at the initiation of treatment and 6 months after treatment.Cephalometric analysis was used to examine the dental and skeletal changes.Results:Significant changes of maxillary anterior displacement and mandibular downward and backward rotation were observed in the seventeen patients:SNA increased 2.6?,A point moved forward 3.1 mm. The overjet correction of anterior teeth was 5.2 mm, 63% of the overjet correction was due to skeletal changes and 37% due to dental changes. Conclusion:Traction arch combined with protraction is a simple and effective apparatus in the treatment of Angle Ⅲ malocclusion.

ObjectiveTo summarize the application experience of laparoscopic totally extraperitoneal prosthetic(TEP) for inguinal hernia in grass-root hospitals.MethodsThe clinical data of 63 cases of inguinal hernia treated by TEP were retrospectively analyzed,and operation scheme suited to the actual conditions of grass-root hospitals was summed up.ResultsAmong the 63 cases,5 cases were treated by transabdominal preperitoneal repair of inguinal hernia(TAPP) to complete the operation because of relatively serious peritoneal rupture.No case was converted to open surgery.11 cases were subjected to continuous epidural anesthesia and the other cases to endotracheal anesthesia.17 cases gained satisfactory result withoutstapling fixation.Among them,a few people had postoperative complications:3 cases of pneumoscrotum,1 case of hematoma of scrotum and 2 cases of early groin pain.No case recurred.ConclusionTEP is a safe and effective operation method in grass-root hospital for inguinal hernia repair.A reasonable selection can be taken according to the individual condition of patients including operation,anaesthesia,mesh style and stapling fixation,to ensure the operation quality,to reduce the medical wst and to reduce the economic burden of the patients.

In this study ,we intended to prepare anti‐Toxoplasma gondii aquaporin (TgAQP) peptide antibody which was used to the application in the detection of the aquaporin expression and its subcellular localization of Toxop lasma gondii (T .gondii) ME49 strain .The B cell peptide antigen was designed based on the TgAQP amino acids sequence .After the pep‐tide antigen was conjugated to the KLH ,the fusion antigen was injected into New Zealand rabbits to prepare polyclonal anti‐body ,followed by identification of ELISA ,Western‐blotting and immunofluorescence assays .The ELISA showed that the titer of anti‐TgAQP antibody was about 1∶40 000 .Western blotting revealed the specific affinity of the antigen to polyclonal anti‐body at 29 .9 kDa protein T .gondii .The protein detected by the indirect immunofluorescence assays was distributed in the cy‐toplasm of the parasite .Thus far ,the anti‐TgAQP polyclonal antibody was successfully prepared ,providing a useful tool for further study of biological function and metabolic characteristics of TgAQP .

Objective To investigate the validity and reliability of Item Erectile Function Scale (I-EF) for neurogenic erectile dysfunc-tion (ED). Methods 104 patients with spinal cord injury were assessed with I-EF. They were measured with pudendal somatosensory ner-vous evoked potential (SEP), bulbocavernosus reflex potential time (BCR) and nocturnal penile tumescence (NPT) detection. 34 patients ac-cepted medicine for ED, and were assessed with I-EF after treatment. 21 patients with medium ED were assessed by 2 testers, and retested 2-4 weeks later. Results The patients who recorded their potential of SEP and BCR got the scores of Q1, Q2 above 7, and the absence with the scores of 3. Those found NPT 1-3 times got the score of Q3 above 7, and the absence with the scores of 3. The incidence of reflex erec-tions and NPT were more in complete suprasacral ones than in complete sacral ones (P<0.05). There was significant improvement after med-ication in patients with mild to medium ED (P<0.05). The interclass correlation coefficient (ICC) was 0.93 between testers and 0.90 between test-retest. Conclusion I-EF can be used to assess neurogenic erectile dysfunction for rehabilitation.

Objective To evaluate the effectiveness and safety of Botulinum toxin-A (BTX-A) injection into detrusor to treat neurogenic detrusor overactivity in patients with spinal cord injury (SCI).Methods A total of 78 patients with SCI were treated with transurethral injection of BTX-A (300 IU dissolved in 15 ml of saline) into 30 different points of detrusor with 15 ml in every patients. Urodynamic parameters and voiding diary were assessed at baseline and 3 weeks and 3 months after the injections. Adverse events were recorded after the injection if present.Results After the first injection, 78 patients showed that the mean frequencies of incontinence decreased from 13.5 to 2.7 times per day, the mean volume of intermittent catheterization (IC) increased from 131 ml to 389 ml per time, the mean volume of incontinence decreased from 1 690 ml to 281 ml per day, the mean getting effect time was 7.6 days. 10 patients received second injection at 8.9 months after first injection, the results showed that the mean frequencies of incontinence decreased from 9.7 to 3.7 times per day, the mean IC volume increased from 108 ml to 387 ml. 6 patients received third injection at 5.8 months after second injection, the results showed that the mean frequencies of incontinence decreased from 9.2 to 3.9 times per day, the mean IC volume increased from 116 ml to 364 ml. No side effects were observed during the follow-up.Conclusion BTX-A injection into detrusor to treat neurogenic detrusor overactivity in patients with SCI seems to be an effective, safe and miniinvasive solution.

Objective To compare and explore the curative effects of elective operation and emergency operation in treating atlantoaxial vertebral segmental spinal canal space-occupying lesions.Methods Thirty-two patients suffering from atlanto-axial vertebral segmental spinal canal space-occupying lesions treated in our hospital from May 2010 to April 2015 were selected and divided into the emergency operation group (group A,n =14) and elective operation group (group B,n =18).The emergency and elective operations were adopted respectively.Then the operation time,intraoperative blood loss,JOA score,ODI index,VAS score,postoperative imaging(MRI) and effect satisfaction degree were compared between the two groups.Results After treatment,the JOA score in the group A was (25.23±4.47) points,which was higher than (22.10±3.56) points in the group B,and the difference was statistically significant (t=3.67,P＜0.05).The ODI index and VAS score of the two groups all were decreased.The ODI index in the group A was (18.56±3.10) points,which in the group B was (21.56±4.37) points,and there was statistically significant difference between the two groups (t=3.76,P＜0.05).The VAS score in the group A was (1.89 ±-0.53)points,which in the group B was (3.16±0.89)points,the difference was statistically significant between the two groups (t=3.76,P＜0.05).Before surgery and at postoperative 1 month,the spinal cord function classification(Frankel grade) of the two groups had no statistically significant difference between the two groups(Z=-0.18,P=0.85＞0.05,Z=-0.52,P=0.60＞0.05).The operation time had no statistical difference between the group A and B[(120.23±9.02)min vs.(126.25±12.12)min,P＞0.05].The intraoperative bleeding volume had had no statistical difference between the group A and B [(211.26±12.25)mL vs.(220.43±17.58)mL,P＞ 0.05].After one month of treatment,the satisfaction degree in the group A was 92.56 ％,which was higher than 72.22％ in the group B,and the difference was statistically significant (Z=-2.13,P＜0.05).Conclusion Emergency operation in treating atlantoaxial segment spinal space occupying lesions can effectively improve the therapeutic effect,and has higher patients satisfaction after treatment.Therefore which is worth promoting and applying.

Objective To explore the technique, efficacy and complications of transurethral sphincterotomy with Holmium laser for the treatment of male patients with neurogenic bladder and examine its long-term outcome. Methods 20 male cases who received transurethral sphincterotomy with Holmium laser were included in this retrospective analysis, in which 7 cases injuried at cervical spinal cord, 6 at thoracic spinal cord, 3 at cauda equine; 1 was spinal meningocele, 1 was sacral crack, 1 was ependymoma, and 1 was myelitis sequel. The age was 20~64 years, averaged 45.1 years. They were followed up for 3~95 months (averaged 43.6 months) since the last sphincterotomy. Results The 20 patients significantly improved in symptoms, laboratory examination, imaging demonstration and urodynamic index after the operation.There were 3 patients who required repeated laser surgery during the follow- up. 17 patients were stable. Conclusion Transurethral sphincterotomy with Holmium laser is effective on neurogenic bladder with less bleeding for the appropriately selected patients.