OBJECTIVE:To observe the short-term efficacy and toxicity reaction of 2 regimens of taxotere combined with epi-rubicin in the neoadjuvant chemotherapy of breast cancer. METHODS:78 patients with locally advanced breast cancer were random-ly divided into TEC group(45 cases)and TE group(33 cases). TEC group was treated with taxotere 75 mg/m2 by intravenous in-jection,d1+epirubicin 60 mg/m2 by intravenous injection,d1+cyclophosphamide 500 mg/m2 by intravenous injection d1. TE group was treated with taxotere 75 mg/m2 by intravenous injection,d1+epirubicin 60 mg/m2 by intravenous injection,d1. 21 days were a treatment course for 2 groups,and there were totally 4-6 courses. In the 2 groups,efficacy and toxicity reaction were evaluated af-ter 4 weeks at least,and survival rate of 6 months was followed-up. RESULTS:The differences were not statistically significant in the short-term efficacy and toxicity reaction and survival rate between 2 groups (P>0.05). CONCLUSIONS:TEC and TC have similar short-term efficacy and safety in the treatment of locally advanced breast cancer. Both of them can be used as adjuvant medi-cines for neoadjuvant chemotherapy.

Objective To study the relationship of secretory type Ⅱ phospholipase A2(sPLA2),coronary artery score and atherogenic index in patients with coronary artery disease.Methods One hundred and seventy nine patients confirmed by angiography were enrolled in the coronary heart disease(CHD)group and another 89 non-CHD patients were enrolled in the control group.Serum levels of sPLA2 were measured by ELISA in all subjects.The severity of coronary artery lesions was analysed in terms of coronary artery score by quantitive computer system(QCA).The atherogenic index(AI)was also evaluated in the 2 groups.Results Compared with the control group,serum level of sPLA2 was higher in the CHD group(55.18?11.75 u/mL vs 68.15?16.70 u/mL,P

Objective To analyze the causes of inappropriate discharge of implantable cardioverter defibrillator (ICD) in elderly patients and to discuss the corresponding solutions.Methods Totally 95 elderly patients with ICD were collected.They were followed up and the inappropriate ICD discharges were recorded and analyzed.Results All patients were followed up for 235 times/year totally.There were 16 patients with inappropriate ICD discharges.The causes of inappropriate ICD discharges included ventricular oversensing in 7 patients (43.7％),supraventricular tachycardia misclassified into ventricular tachycardia in 4 patients (25.0％),electrode fracture in 2 patients (12.5％),and electromyogram (EMG) interference,electromagnetic interference and electrode fracture with EMG interference in 1 patients respectively (6.3％ each).11 inappropriate ICD discharges were reduced and avoided by reprogrammed and noninvasive treatment in 16 patients.1 patient with the underdetected ventricular fibrillation after reprogramming and the problem was solved by external defibrillation.The other 5 patients got better by the invasive treatment.Conclusions Different causes account for inappropriate discharges in patients with ICD.Reprogramming the corresponding parameters in different ICD can reduce or avoid inappropriate discharges without affecting the sensitivity of ICD.

Objective To explore the diagnostic value of dilated intercellular space detected by light microscope for non-erosive reflux disease (NERD) and erosive esophagitis (RE). Methods A total of 104 subjects were divided into normal control group (n = 20), NERD group (n = 30) and RE group (n = 54).Biopsies were taken at 2-3 cm above the dentate line and were examined by light microscope to calculate the intercellular space and compared between different groups. Results The mean values of intercellular space in RE ( 1.40 ±0. 17 μm) and NERD ( 1.11 ± 0. 14 μm) were significantly higher than that in control group (0.66±0. 18 μm, x2 = 154. 170, P =0.000). But no significant difference was noted between RE and NERD groups ( t = 0. 044, P = 0. 834). The cut-off value of mean intercellular space with light microscope was 0. 89 μm, with sensitivity and specificity at 95.2% and 95.0%, respectively. Conclusion Dilated intercellular space under light microscope can be a sensitive, specific and objective indicator of NERD.

Objective To investigate the effect and mechanisms of factor fibroblast growth factor inducible 14(Fn14)in the high glucose induced-cardiomyocyte hypertrophy. Method To observe the expression of collagenⅠ, connective tissue growth factor ( CTGF ) , transforming growth factor-β1 ( TGF-β1 ) , and Fn14 in high glucose induced-cardiomyocyte hypertrophy. Fn14 expressions was down-regulated by siRNA interference technique, and then the expressions of collagen Ⅰ, CTGF, and TGF-β1 were observed, and the mechanism was also explored. Results The expression of collagen I, CTGF and TGF-β1 was significantly up-regulated after high glucose induced-cardiomyocyte hypertrophy for 72 h. At the same time, the expression of Fn14 was increased after 72 h-treatment, and reached the peak at concentration of 30 mmol/L high glucose. High glucose could not up-regulated the expression of collagenⅠ, CTGF, and TGF-β1 after siFn14 interference, while the same result was observed in the expression of p-JNK. Conclusion The expressions of collagenⅠ, CTGF, TGF-β1, and Fn14 in cardiomyocyte of neonatal rats were induced by high glucose. While Fn14 expression was inhibited, the expressions of collagenⅠ, CTGF, and TGF-β1 were down-regulated, which seems to be involved with p-JNK signaling pathway.

Objective To investigate the correlation between serum adiponectin level and severity of coronary artery lesion. Methods The serum concentrations of adiponectin in 112 patients whom had received coronary arteriongraphy (CAG) were measured. Among them,58 cases had acute coronary artery syndromes(ACS)(ACS group), 25 cases had stable angina cordis(stable angina cordis group) and 29 cases had non-coronary disease (control group ). The severity of coronary artery lesion was evaluated by the number of stenosis vessel and Gensini score. All the patients were divided into single vessel lesion group (24 cases),double-vessel lesion group (26 cases ), triple-vessel lesion group (33 cases) by extent of disease; and 0- 1 score group (29 cases),2-20 scores group (28 cases),21-40 scores group (30 cases), ＞40 scores group (25 cases) by Gensini score. Results The serum concentrations of adiponectin in ACS group were lower than those in control group and stable angina cordis group[(6.43 ± 4.22 ) mg/L vs. ( 12.68 ± 6.47 ), (9.94 ± 8.48 ) mg/L]( P ＜ 0.01 or ＜ 0.05 ). The serum concentrations of adiponectin in control group were higher than those in single vessel lesion group, double-vessel lesion group and triple-vessel lesion group[( 12.68 ± 6.47 ) mg/L vs. (8.80 ±6.23), (8.04 ±5.93), (6.43 ±5.12) mg/L](P ＜0.01). The serum concentrations of adiponectin in single vessel lesion group were higher than those in triple-vessel lesion group (P＜ 0.01 ). The serum concentrations of adiponectin in 0-1 score group[.( 12.68 ±6.47) mg/L]and 2-20 scores group [(8.74 ± 6.68) mg/L]were respectively higher than those in 21-40 scores group[(7.64 ± 5.32) mg/L]and ＞ 40 scores group[(6.32 ± 5.46 ) mg/L](P ＜ 0.01 ). The serum concentrations of adiponectin in 21-40 scores group were higher than those in ＞ 40 scores group(P＜ 0.05 ). The serum concentrations of adiponectin were negatively correlated with the log of Gensini score (r = -0.584,P ＜ 0.01 ). Conclusion The decrease in serum adiponectin might reflect the increase in severity of coronary artery lesion.

ObjectiveTo investigate the correlation between serum uric acid level and severity of coronary artery disease.MethodsThe concentrations of serum uric acid in 112 patients whom had received coronary arteriongraphy(CAG) was measured,among of them,acute coronary artery syndrome (ACS) with 58 cases( ACS group ),stable angina cordis with 25 cases( stable angina cordis group),non-coronary disease with 29 cases(control group).The severity of coronary artery lesion was evaluated by the number of stenosis vessel and Gensini score.They were divided into control group (29 cases),single vessel lesion group (24 cases),double vessel lesion group(26 cases),triple vessel lesion group(33 cases ) according to the number of stenosis vessel and 0-1 score group(29 cases),2-20 scores group(28 cases),21-40 scores group(30 cases),＞40 scores group (25 cases) according to the Gensini score.ResultsThe concentrations of serum uric acid in ACS group were higher than those in control group and stable angina cordis group[ (369.61 ± 91.97 ) μ mol/Lvs. (298.33 ±92.46),(330.43 ±87.42)μmol/L] (P ＜0.05).The concentrations of serum uric acid in control group were lower than those in single vessel lesion group,double vessel lesion group and triple vessel lesion group [(298.33 ±92.46)μmol/L vs. (331.77 ±86.33),(368.24 ±95.21),(396.82 ±94.45) μ mol/L] (P ＜ 0.05).The concentrations of serum uric acid in single vessel lesion group were significantly lower than those in double vessel lesion group and triple vessel lesion group (P ＜ 0.05 ).The concentrations of serum uric acid in 0-1 score group [ (298.33 ± 92.46) μ mol/L] and 2-20 scores group [ (320.77 ± 86.33 ) μ mol/L ] were respectively lower than those in 21-40 scores group [ (366.61 ± 91.97 ) μ mol/L ] and ＞ 40 scores group [ (402.82 ± 91.97 ) μ mol/L](P ＜ 0.05 ).The concentrations of serum uric acid in ＞ 40 scores group was higher than that in 21-40 scores group (P ＜ 0.05 ).Serum uric acid concentrations was positively correlated with the log of Gensini score (r =0.348,P ＜ 0.05 ).ConclusionThe increase in serum uric acid might reflect increase in severity of coronary artery stenosis.

To evaluate the efficacy and safety of new drugs of traditional Chinese medicine (TCM) for acute upper respiratory tract infection (common cold).

The multiplex polymerase chain reaction (PCR) technique was applied to detect the SARS-CoV (severe acute respiratory syndrome-associated coronavirus) specific target cDNA fragments in the present study. The target cDNA fragments of SARS-CoV were synthesized artificially according to the genome sequence of SARS-CoV in GenBank submitted by The Chinese University of Hong Kong, and were used as simulated positive samples. Five primers recommended by World Health Organization (WHO) were used to amplify the fragments by single PCR and multiplex PCR. Three target cDNA fragments (121, 182 and 302 bp), as well as the three different combinations of any two of these fragments, were amplified by single PCR. The combination of these three fragments was amplified by multiplex PCR. The results indicated that the multiplex PCR technique could be applied to detect the SARS-CoV specific target cDNA fragments successfully.
Base Sequence ; Cloning, Molecular ; DNA Primers ; DNA, Complementary ; genetics ; Molecular Sequence Data ; Polymerase Chain Reaction ; methods ; SARS Virus ; genetics

Objective To investigate the clinical value of radiomics nomogram,which is established by 18F-fluorodeoxyglucose (FDG) PET/CT radiomics signature combined with clinical-pathologic risk factors,in predicting the prognosis of patients with postoperative gastric carcinoma.Methods 18F-FDG PET/CT data of 207 patients (143 males,64 females,age range:20-85 years) with postoperative gastric carcinoma from January 2008 to August 2015 was reviewed retrospectively.Patients were divided into training group (n=104) and validation group (n =103),and the clinicopathologic information and disease-free survival (DFS) data were acquired.Significant textural features were selected from PET/CT images,and radiomics score (RS) for individual patient was calculated based on the radiomics signatures.The relationship between RS and DFS was analyzed.Cox regression analysis was performed to determine the risk factors ofDFS.The radiomics nomo-gram,obtained from combination of RS with clinicopathologic risk factors,was established and further evaluated in predictive value for recurrence or metastasis of postoperative gastric carcinoma,and the concordance index (C-index) was calculated.Results Cox regression analysis demonstrated that RS,tumor location,depth of invasion,lymph node metastasis,and distant metastasis were the significant risk factors for DFS (hazard ratios:2.148-2.828,all P＜0.05).The radiomics nomogram combined with RS and 4 clinicopathologic risk factors had a better prediction for the estimated DFS,comparing to RS alone.C-index of radiomics nomogram and RS were 0.830 and 0.700 in training group,and 0.776 and 0.681 in validation group,respectively.Conclusion Radiomics nomogram which is established by radiomics signatures and clinicopathologic risk factors may be better for predicting DFS of patients with postoperative gastric carcinoma.