0.05). Conclusions With intravenous injection of low-dose combination, droperidol and fentanyl can produce effective, painless and safe sedation for TEE examination.

Tumor thermal ablation therapy has been the common minimally invasive technique because of its excellent efficacy and safety,and has become a promising clinical minimally invasive treatment.But there are still limitations in its therapeutic range.Moreover,as an effective local treatment,the technology is bound to a cooperation with tumor system treatments.This review provides an overview of the concept of tumor thermotherapy and thermal ablation,and the possible mechanism of the synergistic effect of the thermal ablation with chemotherapy,including hyperthermia changing the blood supply to improve drug distribution,hyperthermia increasing drug intake,hyperthermia modifying the cytotoxicity of many chemotherapeutic agents,combination therapy improving the rate of complete ablation and promoting cell apoptosis.

Purpose To study the correlation between the expression of podoplanin-positive stromal cells and clinicopathologic features in squamous cell carcinoma ( SCC) of esophagus, and to explore the role of podoplanin-stromal cells in the progression of SCC of e-sophagus. Methods Immunohistochemical EnVision method was used to detect the expression of podoplanin in the stromal cells of e-sophageal SCC. The correlation between the expression of podoplanin-positive stromal cells and clinicopathologic features in SCC of e-sophagus was analyzed. Results Of 69 cases of SCC, podoplanin-positive stromal cells were seen in 43 and the positive rate was 62. 3%. Lymphatic vessel density in SCCs with podoplanin-positive stromal cell was 45. 72 ± 14. 36, which was higher than that with-out podoplanin-positive stromal cell (21. 23 ± 10. 49) (P<0. 01). Expression of podoplanin in stromal cells in SCCs with lymphatic vessel invasion was higher than those without lymphatic vessel invasion (P<0. 05). There was no correlation between podoplanin-posi-tive stromal cells and age, sex, tumor size, the depth of invasion, differentiation, lymph node metastasis, and clinical stage ( P>0. 05). Conclusion Podoplanin-positive stromal cells may promote the lymphangiogenesis and lymph vessel invasion of esophageal SCC.

Objective: Patient-reported outcome (PRO) method was used to evaluate clinical effects of acupuncture for cervical spondysosis. Methods: With RCT method used, 162 cases were adopted, and 150 cases finished the whole course (treatment group: 76 cases, control group: 74 cases). Treatment group cases were treated by real-acupuncture while control group cases treated by sham-acupuncture. Northwick Park Pain Questionnaire(NPQ)was used as main outcome index, while SF-36 as secondary index. Both groups’ patients were evaluated before and after treatment course, plus one month and 3 months after the end of the treatment course. Results:Treatment group cases got lower NPQ Scores and higher mental health dimensions scores than the control group cases at any evaluation time-point. At the point of one month after the end of the treatment course, the treatment group cases got better social function scores than the control group cases. At the point of three months after the end of the treatment course, the treatment group cases got better body pain and energy scores than the control group cases. Multiple regression analysis showed that three dimensions of SF-36 (social function scores body pain and energy scores) had close relationship with NPQ scores. Conclusion: Acupuncture had satisfactory long-term effect for cervical spondylosis.

Objective:To improve the identification and determination methods in the original quality standard for belladonna oral solution.Methods:Belladonna oral solution was identified by a specific chromatogram of HPLC,and scopoletin and hyoscyamine sulfate in belladonna oral solution were detected by dual wavelength spectrophotometry.The detection of fingerprints was performed on a Waters SunFire C18 (250 mm×4.6mm,5 μm) column.The mobile phase was methanol-0.05% phosphoric acid solution at a flow rate of 1.0 ml·min-1.The detection wavelength was 344 nm and the column temperature was 30℃.The detection of scopoletin and hyoscyamine sulfate was performed on the same C18 column.The mobile phase was 10 mmol·L-1 heptanesulfonate sodiumat (pH value was 3.3 adjusted by glacial acetic acid)-absolute ethanol-acetonitrile (68.75∶6.25∶25) at a flow rate of 1.0 ml·min-1.The detection wavelengths were 344 nm and 210 nm,and the column temperature was 30℃.Results:The specific chromatogram of belladonna oral solution was accordance with that of belladonna tincture raw material.The retention time and relative peak area of each characteristic peak and reference peak all met requirements of Chinese Pharmacopoeia.Scopoletin and hyoscyamine sulfate were completely separated from the other compositions under the above mentioned conditions.The calibration curves were linear within the range of 5.168-103.360 μg·ml-1 (r=1.000 0) for scopoletin and 50.560-758.400 μg·ml-1 (r=0.999 9) for hyoscyamine sulfate.The average recovery was 101.79% (RSD=1.05%,n=6) and 100.92% (RSD=0.97%,n=6),respectively.Conclusion:After the quality control method improvement,the identification shows high specificity and the quality of belladonna oral solution can be better controlled by the two selected index components.The method is easy and accurate,which can provide a reliable way to control the quality.

Background The severity of difficulty in falling asleep and the insufficiency of the sleeping time in the primary insomniacs always were overestimated, even after medication and improving the objective sleep quality. Moreover, remarkable psychosomatic symptoms were found in patients and the symptoms were significantly linked to the self-reported sleep quality. So in this study, it was designed to explore the differences between objective and subjective sleep quality and its correlative factors in chronic insomniacs.Methods A total of 55 chronic primary insomniac cases diagnosed by DSM-Ⅳ diagnostic criteria for primary insomnia and 15 normal controls were rated with Pittsburgh sleep quality index (PSQI),State-Trait Anxiety Inventory (STAI), and the Subjective General Condition Form. All cases and the controls were then examined by the whole-night polysomnography (PSG) records.Results Compared with the objective parameters of PSG, the subjective parametersiu chronic insomniacs had significantly longer sleep latency and lower total sleep time and sleep efficiency [ (80. 3± 73.7)min v.s. (23.2 ± 25.4) min, (157.8±141.7)min v.s. (332.2±154.7)min , (0.52 ±0. 27) min v.s. (0. 67 ± 0. 28) min, respectively, P ＜0. 001 ]. The difference between subjective and objective sleep latency was related to the total scores of STAI, TAI and SAI ( r = 0. 402,0. 374 and 0. 397, respectively, P ＜ 0. 05).Conclusions There were significant differences between objective and subjective estimations of sleep quality in chronic insomniacs,and they might result in the overestimation of the severity of insomnia. The differences between subjective and objective sleep quality in the chronic insomniacs were significantly correlated with the levels of anxiety.

Objective To study the effect of sodium cantharidinate on the growth of human gastric cancer grafted onto nude mice. Methods Nude mice xenograft models of human gastric cancer were established.BGC823was injected peritoneal and the mice were weighed. The proliferating and apoptosis rates of xenografta was evaluated by TUNEL assay and immunohistochemical staining respectively. Results The xenografts were obviously inhibited with various dosage of sodium cantharidinate(P＜0.01 ), the proliferating rate of turnout cells after using sodium cantharidinate was lower than that before using sodium cantharidinate(P＜0.01 ), but apoptosis rate of tumour ceils after using sodium cantharidinate was higher than that before using sodium cantharidinate(P＜0.01 ). Conclusion Sodium cantharidinate can inhibit gastric cancer growth by inhibiting tumour cell proliferating or inducing cell apoptosls.

Objective　To investigate the effect of intratumor injection of 131I-3H11 for gastric cancer (GC). Methods　16 patients with GC subjected to endoscopic intratumor injection of 131I-3H11 as a treatment group; 6 GC patients with FAM chemotherapy as a control group. Histological examination of the postoperative specimens of the two group were comparated. Results　In treatment group, 75.0%(12/16) of cases were found to have morphological changes with karyopyknosis, karyorrhexis, coagulation of cytoplasm, and invasion of lymphocyte in mesochyma. Most of these changes were medial; but in control group no obvious morphological change was found. Conclusions　The results suggest that GC subjected to endoscopic intratumor injection of 131I-3H11 preoperatively has promising application in the clinic.

Adenocarcinoma ; metabolism ; pathology ; Atrophy ; Biomarkers ; metabolism ; Diagnosis, Differential ; Humans ; Male ; Prostate ; pathology ; Prostatic Diseases ; metabolism ; pathology ; Prostatic Hyperplasia ; metabolism ; pathology ; Prostatic Neoplasms ; metabolism ; pathology ; Prostatitis ; metabolism ; pathology ; Xanthomatosis ; metabolism ; pathology

Carcinoma, Renal Cell ; classification ; pathology ; Humans ; Kidney Neoplasms ; classification ; pathology ; Terminology as Topic