Objective: To establish a HPLC method for the determination of synephrine in Dachengqi Granules (Radix et Rhizoma Rhei, Fructus Aurantii Immaturus, Cortex Magnoliae Officinalis, Natrii Sulfas). Methods: A E. Merck LiChrospher 100RP 18 column was /used. The mobile phase was methanol water (contained 0.18% H 3PO 4 and 0.22% C 12 H 25 SO 4Na) (55∶45). The detection wavelength was at 274nm.Results: The linear range of synephrine was 0.14?g～1.54?g, r =0.9998. The average recovery was 98.1%, RSD =2.1% ( n =5).Conclusion: The result is accurate and the reproducibility is good. This method can be used for the determination of synephrine in Dachengqi Granules.

[Objectivs] To investigate the principle of AgNO3-processed silica gel chromatography in isolation and purification of mixtures with carbon-carbon double bonds (CCDB) from herbal medicine. [Methods] AgN03-processed gel chromatography was used for the isolation of mixtures with carbon-carbon double bonds in the volatile oils and active components from Pteris semipinnata L. ; silver-ion complexion chromatography was applied to isolate arachidom'c acid from lipids. The principle of AgNO3-processed silica gel chromatography was analyzed by coordination theory. [Results] Stable AgNO3 coordinate complexes were obtained. [ Conclusion ] The principle of the formation of stable AgNO3 coordinate complexes may be: (1) the stability will increase with CCDB in free space; (2) exocyclic CCDB is more stable than that of intra-cyclic CCDB; (3) cis-form CCDB is more stable than that of trans-form CCDB; (4) terminal CCDB is more stable than that of cis-form CCDB; (5) aromatic hydrocarbon with multiple benzene rings is more stable than that with single ber zene ring. The possible principle will benefit to the isolation and purification of the mixtures with CCDB, which have similar structure and physiochemical property and are difficult to be isolated and purified by common chromatography and extriction method.

The incidence of thyroid carcinoma has been increasing in recent years.More and more researchers have paid attention to this field and great achievements have been made year by year.The developments on gene diagnosis and molecular markers of thyroid carcinoma are reviewed in this article.

Objective To establish the standard for the quality control of Compound Huangqin Tablets. Methods The identification of Radix Scutellariae,Rhizoma Polygoni Cuspidati and Caulis Mahoniae in compound Huangqin tablets were carried out by TLC.The content of baicalin in the preparation was determined by HPLC.Chromatocolumn was adopted,methanol-0.4 %H3PO4(50 ∶50) used as the mobile phase,flowrate 1.4mL/min,column temperature at 40 ℃and detection wavelength 278 nm . Results The TLC spots were highly clear without the interference of negative control and were reproductive. The calibration curve for baicalin was linear (r=0.9996) in the range of 0.12～0.72 ?g and the mean recovery was 97.17 %and RSD=1.78 %(n=5). Conclusion This method can be used for the quality control of Compound Huangqin Tablets.

Objective To evaluate the value of imaging examination and diagnosis of airway foreign body in children. Methods 218 of airway foreign bodies confirmed by bronchoscopy were retrospectively analyzed in terms of clinical and imaging data. Results 218 patients included 140 males and 78 females. Their ages ranged 6 months to 12 years(mean,19.2 months). All patients had conventional radiographs on frontal chest films,neck lateral films and fluoroscopy. 34 cases underwent 64-slice spiral chest CT examination. According to the location of the foreign bodies,29 cases were in throat and subglottic,55 cases in trachea and 134 cases in bronchus(79 cas-es in the right,55 cases in the left). According to the character of the foreign bodies,5 cases were metal,29 cas-es in bone,93 cases in peanuts,59 cases in seeds,22 cases in fruits and vegetables and 10 cases in others respec-tively. Thirty-fourcases showed a direct sign of the foreign body and 177 cases showed the indirect signs. 34 cases on CT examination directly were showed foreign body in the tracheal bronchi. The diagnostic accuracy of CT exami-nation was 100%. Conclusions X-ray photograph and fluoroscopy were the first choice for aspiratory foreign bod-ies in children. CT examination is an important supplementary method when conventional X-ray examination is neg-ative.

The identification of Radix Morindae Officinalis, Radix Rehmanniae Preparata, Herba Epimedii, Radix Ginseng, Radix Achyranthis Bidentatae, Rhizoma Dioscoreae in Baji Bushen decoction was carried out respectively by TLC. The content of icariin in the preparation was determined by HPLC. Chromatocolumn was adopted, methanol - 0. 5 % HClO4 (55: 45) as the mobile phase, flow - rate at 0.8 mL/min, column temperature at 40℃, detection wavelength at 270nm, the mean recovery is 97. 36%and RSD is 2.61% (N=5). It is indicated that the methods can be used for the quality control of Baji Bushen decoction.

Objective To explore the diagnostic value of MSCT in tumors of intra-abdominal cryptorchidism in children.Methods MSCT findings of 8 children with tumors of intra-abdominal cryptorchidism confirmed by surgery and pathology were analyzed retrospectively.Results Six tumors located in the right,2 (1 tumor of left cryptorchidism turned to the right abdominal) in the left.Eight children showed ovoid soft tissue tumor in abdomen.Three children displayed the long axis of the tumors consistent with regular descending course of embryonic testes.Six teratomas manifested as the cystic and solid mass with fat,calcification (ossification) insidey.Two yolk sac tumors manifested as the large cystic and solid mass with irregular necrosis and abundant tumor vessels.Conclusion The pathologic types of tumors for intra-abdominal cryptorchidism in children are different from adult.Most of them are teratomas or yolk sac tumors,and have some characteristics in MSCT.MSCT is helpful in diagnosis of tumor for intra-abdominal cryptorchidism with medical history.

OBJECTIVE:To extract the feature of Achyranthes bidentata and Cyathula officinalis,and to establish image recog-nition method. METHODS:The microscopic image stitching of A. bidentata and C. officinalis was implemented by MATLAB. The color,invariant moment,stripes and the features of vascular bundle in cross section were extracted. The data was organized into da-ta matrix,and then data matrix was standardized by Zscore function;principal components were analyzed through Princomp func-tion. BP nerve network recognition mode was adopted. RESULTS:The microstructures in the micro images of the samples were kept integrated. The measured data of 27 characteristics were acquired in each group of sample. Through principal component analy-sis,the parameters of 11 main components were selected to establish BP never network. The average recognition rate of BP nerve network was 100% between 2 medicinal material relatives (n=50). CONCLUSIONS:The method can be used for micro-image auto Stitching of Chinese medicinal materials and image recognition of A. bidentata and C. officinalis.

Objective To evaluate the application value of MSCT in preoperative diagnosis and postoperative evaluation in children with cavernous transformation of the portal vein (CTPV).Methods 11 children diagnosed with CTPV by surgery were included in this study in our hospital.All patients underwent both plain and enhanced MSCT scans before surgery,and ultrasound or MSCT scan after surgery.Results In all 11 cases,preoperative CT showed different degrees of splenomegaly and the plain CT showed abnormal soft tissue mass in the hepatic hilar area.Enhanced CT showed stenosis or occlusion of portal vein trunk,earthworm-like blood vessels in the hilar region,and that the left and/or right branch of intrahepatic portal vein were replaced by multiple densely-distributed blood vessels (typeⅡ). Superior mesenteric-intrahepatic left portal shunt (Rex surgery)was performed in all 11 cases with successful results.All patients were followed up for 2-20 months after surgery.Imaging examinations showed anastomotic patency in 9 cases and stenosis in 2 cases.Conclusion MSCT findings of CTPV in children have certain characteristics.MSCT plays an important role in preoperative diagnosis and postoperative evaluation of CTPV in children.

Objective To analysis the correlation of serum clozapine concentration with serum homocysteine (Hcy) level and biochemical index in schizophrenic patients. Methods Two hundred and forty one schizophrenic patients treated with clozapine in Beijing Anding Hospital from January 1st 2017 to January 31st 2018 were enrolled in the study. Serum clozapine concentration, Hcy levels and biochemical index were measured. Patients were divided into high concentration group (n=41), normal concentration group (n=86) and low concentration group (n=114) according to serum clozapine concentration. The correlation of serum clozapine concentration with Hcy level and biochemical index was analyzed. Results The levels of Hcy, TG and LDL were higher in high clozazapine concentration group than in low concentration group and in normal concentration group (P<0.05). There was no significant difference between normal group and low concentration group in Hcy, TG and LDL levels (P>0.05). High serum clozapine concentration was positively correlated with serum Hcy (r=0.221, P=0.013), TG (r=0.193, P=0.003) and LDL level (r=0.148, P=0.021). Conclusion Serum levels of Hcy, TG and LDL in schizophrenic patients treated with clozapine are correlated with drug concentration, suggesting that patients with abnormally elevated levels of Hcy, TG and LDL should be monitored.