Objective To observe the effect of acetylated low-density lipoprotein (AcLDL) on the expression of adipophilin and the effect of adipophilin on AcLDL uptake and lipid accumulation in human vascular smooth muscle cells (HVSMCs)in order to approach the role played by adipophilin in genesis of macroangiopathy. Methodse HVSMCs were treated with various amount of AcLDL. Adipophilin expression levels were detected by Northern blot and Western blot. The effects of adipophilin on AcLDL uptake and lipid accumulation in HVSMCs were observed by the methods of siRNA, flow cytometry, enzymatic method and oil red stain. Results AcLDL dose-dependently increased adipophilin expression in HVSMCs. Silence adipophilin by siRNA decreased AcLDL uptake (decreasing by 38.7%, P<0. 05) and lipids accumulation (tfiglyceride and total cholesterol decreasing by 30.6% and 29.8% respectively, both P<0. 01) in HVSMCs, Conclusion Adipophilin is able to increase AcLDL uptake and lipid accumulation in HVSMCs, suggesting that it might play a role in enhancing atherosclerosis.

AIM:To study the effect of astragalus polysaccharides(Aps)on cholesterol efflux in THP-1 macrophage-derived foam cells.METHODS:After exposed to Aps at different doses,cholesterol efflux and ABCA1 protein levels in cultured THP-1 macrophage-derived foam cells were determined by a ? counter and flow cytometry,respectively.RESULTS:Aps increased cholesterol efflux in THP-1 macrophage-derived foam cells with dose dependent pattern and resulted in an increase in the expression of ABCA1 protein in THP-1 macrophage-derived foam cells.CONCLUSION:The increase in cholesterol efflux by Aps might be related to the up-regulation of ABCA1.

Objective To explore the diagnostic value of serum EphA5 gene promoter methylation in prostate cancer(PCa).Methods A total of 71 PCa patients admitted to Affiliated Hospital of Xuzhou Medical University from June 2022 to June 2023 were selected as the study subjects,with 49 patients with benign prostatic hyperplasia(BPH)and 20 healthy individuals during the same period as the control groups.The methylation-specific polymerase chain reaction(MSP)technology was used to detect the methylation status of serum EphA5 gene promoter in all study subjects.The relationships between EphA5 gene promoter methylation and clinic pathological parameters were evaluated by the chi-square test.The receiver operating characteristic(ROC)curve was drawn and used to analyze the diagnostic value of combined detection of EphA5 gene promoter methylation and prostate specific antigen(PSA)in PCa.Results The results of MSP showed that the methylation rate(84.5%,60/71)of serum EphA5 gene promoter in PCa patients was significantly higher than those in BPH patients(24.49%,12/49,P＜0.05)and healthy controls(15%,3/20,P＜0.05).The methylation rate of serum EphA5 gene promoter in PCa patients was correlated with TNM staging(P＜0.05)and Gleason score(P＜0.05).The areas under the ROC curve(AUCROC)of PSA and EphA5 gene promoter methylation in the diagnosis of PCa were 0.868(95%CI:0.810-0.925)and 0.800(95%CI:0.723-0.878),respectively.The AUCROC of the combined detection of PSA and EphA5 gene promoter methylation in the diagnosis of PCa could be raised to 0.901(95%CI:0.853-0.950),and its diagnostic efficiency was better than the single detection of each indicator.Conclusion The methylation level of serum EphA5 gene promoter in PCa patients is significantly higher than those in BPH patients and healthy controls,indicating that serum EphA5 gene promoter methylation may serve as a potential biomarker of PCa.

OBJECTIVES: To evaluate the repairing ability of nano-pearl powder bone substitute in rabbit with defect of distal femur bone. METHODS: Thirty-two New Zealand rabbits were randomly divided into four groups: a nano-pearl powder/recombinant human bone morphogenetic protein 2 (rhBMP-2)/hyaluronic acid group, a nano-pearl powder/hyaluronic acid group, a nano-pearl powder group and a blank control group (=8 in each group). A defect with the diameter of 7 mm and height of 10 mm was prepared at the distal femoral metaphysis line of the rabbit.Different bone substitutes were planted, and the effect of repair was evaluated by macroscopic observation, imaging examination, and histopathological examination. RESULTS: The results of imageology showed that: the bone repairing effect in the nano-pearl powder/rhBMP-2/hyaluronic acid group was better than that in the pure pearl powder group and the nano-pearl powder/hyaluronic acid group, and which in the 3 experimental groups was better than that in the blank control group; The results of histology showed that: at the 4th, 8th and 12th weeks after the modeling operation, the speed of bone repair in the nano-pearl powder/rhBMP-2/hyaluronic acid group was faster than that in the pure pearl powder group and the nano-pearl powder/hyaluronic acid group, and which in the blank control group was far slower than that in the 3 experimental groups. The results of immunohistochemistry staining for osteocalcin antibody showed that: the osteogenic effect in the nano-pearl powder/rhBMP-2/hyaluronic acid group was better than that in the pure pearl powder group and the nano-pearl powder/hyaluronic acid group (both <0.05); there was no significant difference between the nano-pearl powder/hyaluronic acid group and the pure pearl powder group (>0.05); however, there was significant difference between the pure pearl powder group and the blank control group (<0.05). According to the staining results of Type I collagen antibody, there was no significant difference in the osteogenic effect between the nano-pearl powder/rhBMP-2/hyaluronic acid group and the nano-pearl powder/hyaluronic acid group (>0.05), but the osteogenic effect in the nano-pearl powder/hyaluronic acid group was better than that in the pure pearl powder group and the blank control group (both <0.05). CONCLUSIONS Nano-pearl powder and its bone substitute can promote the repair of bone defect, and the nano-pearl powder which contains rhBMP-2 has better osteogenic and repairing effect on defect.
Animals ; Bone Morphogenetic Protein 2 ; Bone Substitutes ; Collagen ; Femur ; Humans ; Osteogenesis ; Powders ; Rabbits ; Recombinant Proteins ; Transforming Growth Factor beta