1.Advances in Understanding of the Infection/Replication Mechanisms and Virulence Determinants of the Porcine Reproductive and Respiratory Syndrome Virus.
Xing LIU ; Fengxue WANG ; Yongjun WEN
Chinese Journal of Virology 2015;31(5):585-592
The porcine reproductive and respiratory syndrome virus (PRRSV) can cause reproductive barriers in breeding pigs and respiratory symptoms in piglets. In this review, we summarize research progress of the infection and replication mechanisms of the PRRSV. We also review the virulence determinants of the PRRSV. All these fundamental studies are important for the control and elimination of the PRRSV.
Animals
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Porcine Reproductive and Respiratory Syndrome
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virology
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Porcine respiratory and reproductive syndrome virus
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genetics
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pathogenicity
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physiology
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Swine
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Virulence
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Virus Replication
2.Investigation on occupational skin diseases in glass fiber enterprises and control measures.
Xing-gang WANG ; Yue-wen LIU ; Yue-hua YAN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2010;28(9):684-685
Adult
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Dust
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Female
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Glass
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Humans
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Male
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Middle Aged
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Occupational Diseases
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epidemiology
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Prevalence
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Skin Diseases
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epidemiology
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Young Adult
4.Diagnostic Value of Spiral CT Head and Neck Angiography on Carotid Artery Stenosis in Patients with Acute Cerebral Infarction
Tie WEN ; Xing JI ; Jian WANG ; Meicheng SHEN ; Xiaoqi HUANG
Progress in Modern Biomedicine 2017;17(22):4318-4320,4352
Objective:To investigate the diagnostic value of spiral CT head and neck angiography on carotid artery stenosis in patients with acute cerebral infarction.Methods:A total of 60 patients with acute cerebral infarction(ACI),who were treated in Affiliated Hospital of Yan'an University from January 2014 to January 2016,were selected as observation group,and 60 patients with non-acute cerebral infarction,as control group.The two groups of patients underwent spiral CT head and neck angiography.The degrees of carotid artery stenosis and the distribution of vascular plaques were compared between the two groups.The diagnostic value of spiral CT head and neck angiography in the diagnosis of carotid artery stenosis of the patients with ACI was evaluated.Results:The detection rates of mild and moderate stenosis in the observation group were significantly higher than those in the control group (P<0.05),while the detection rates of severe stenosis and occlusion had no significant difference compared with the control group (P>0.05).The total detection rate of stenosis in the observation group was higher than that in the control group (P<0.05).There were plaques detected in the carotid bifurcation,common carotid artery,internal carotid artery and external carotid artery in the two groups.The total detection rate (69.4%) of the observation group was significantly higher than that (41.2%) of the control group (P<0.05).Conclusion:Application of 128-slice spiral CT can effectively assess the head and neck angiography on carotid artery stenosis in patients with acute cerebral infarction.This method has a higher detection rate from mild to moderate stenosis and bilateral carotid artery plaques in the patients with ACI,and it has application value in the clinical diagnosis and prognosis.
5.Effect of fluoride on apoptosis and DNA damage in OX peripheral blood lymphocytes
Min, WANG ; Hou-juan, XING ; Fei, ZHU ; Shi-wen, XU
Chinese Journal of Endemiology 2008;27(3):272-275
Objective To explore the effect of sodium fluoride on DNA damage and apoptosis on ox peripheral blood lymphocyte cultured in vitro.Methods Using lymphoeytes separation medium lymphocytes were separated and different concentration of NaF(0,4,8,12,16 mg/L)were added into the cultual system of lymphocytes for 24 h.Cell viability was measured by MTT.nuclear changes stained by acridine orange-ethidium bromine staining (AO/EB)were observed under fluoroscope,DNA fragment was measured by agarose gel electrophoresis.DNA damage was detected by alkaline SCGE.The differences between each groups were compared.Results Cells were exposed to 4,8,16 and 24 mg/L NaF in 24 h,cell survival rates,respectively being(73.743±0.552)%,(69.184±0.724)%,(65.736±0.055)%and(63.651±0.287)%,decreased significantly compared to control group.There were distinctive cell apoptosis,evident DNA damage and visible dose-effect relation(R2=0.7456).Conclusions A certain concentration of sodium fluoride lcads to lymphocytes apoptosis and DNA damage.
6.Students' leading functions in the anatomy teaching reform
Xing-lu WANG ; Wen-jing TANG ; Hai-xia HUANG ; Yu-xing SUN ; Shan-quan SUN ;
Chinese Journal of Medical Education Research 2011;10(9):1069-1071
As the main part of the teaching activities,students play an important role in the teaching reform.The students were trained through 3 pathways,“Extending teaching activities from the classroom to the outside”,“Development from basic to clinical knoledge” and “Culturing students' innovative consciousness”,so as to allow them to give full play in teaching reform,to enhance their ability of practice and learning by themselves,to culture their innovative consciousness and to develop students' leading functions in the anatomy teaching reform.
7.Alterations of protein phosphatase-2A in peripheral lymphocytes in mild cognition impairment and Alzheimer's disease
Zhong-Sen QU ; Yong-Bo ZHAO ; Xing-Bin WANG ; Wen-Wen LIU ; Qiao-Shu WANG ;
Chinese Journal of Neurology 2005;0(10):-
Objective To explore the alterations of protein phosphatase-2A (PP-2A) in lymphocytes in mild cognition impairment (MCI) and Alzheimer's disease (AD).Methods The activity PP-2A of was measured by ~(32)p liquid seintillography for incorporated radioactivity in control group(n=11) , the MCI group(n=11),and the AD group(n=11).The expression of PP-2A was determined by Western blot.Results In the control group,the activity of PP-2A (1.01?0.09) and the expression of PP-2A (0.96?0.07) were high while in the MCI group,the activity of PP-2A (0.71?0.12) and the expression of PP-2A (0.80?0.05) were decreased (both P
8.Rupture of primary splenic multilocular mucous cyst accompanied by pseudomyxoma peritonei: report of a case.
Wen QI ; Wen-xiu LIN ; Na DU ; Xiao WANG ; Li-yi GU ; Qian-xing ZHANG
Chinese Journal of Pathology 2013;42(2):129-130
Aged
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CDX2 Transcription Factor
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Cysts
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metabolism
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pathology
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surgery
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Diagnosis, Differential
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Female
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Homeodomain Proteins
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metabolism
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Humans
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Keratin-20
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metabolism
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Mucous Membrane
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pathology
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Peritoneal Neoplasms
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metabolism
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pathology
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surgery
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Pseudomyxoma Peritonei
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metabolism
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pathology
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surgery
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Rupture
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Splenic Neoplasms
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metabolism
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pathology
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surgery
9.Cost-effectiveness Analysis of 4 Kinds of Protease Inhibitor Preventing Hyperamylasemia and Pancreatitis after ERCP of Choledocholithiasis Patients
Guiliang WANG ; Ping QIU ; Linfang XU ; Xing LI ; Ping WEN ; Min GONG ; Jianbo WEN
China Pharmacy 2017;28(14):1880-1884
OBJECTIVE:To evaluate the economics of somatostatin,ulinastatin,octreotide and gabexate preventing hyperamy-lasemia and pancreatitis after endoscopic retrograde cholangiopancreatography (ERCP). METHODS:Medical records of 316 cho-ledocholithiasis patients underwent ERCP were selected from our hospital during Jul. 2008-Apr. 2016,and then divided into blank control group(58 cases),somatostatin group(64 cases),ulinastatin group(65 cases),octreotide group(68 cases)and gabexate group (61 cases) according to the use of protease inhibitor. Before ERCP,blank control group received routine treatment as fast-ing,parenteral nutrition support,acid suppression,etc. Based on it,other 4 drug groups received prophylactic drug use according to package inserts 6 h before ERCP. The incidence of hyperamylasemia and pancreatitis after ERCP,VAS scores 3,24,48 h after surgery and the occurrence of ADR were compared among 5 groups. The cost-effectiveness analysis was used to evaluate the eco-nomics of therapy plans in each group. RESULTS:Compared with blank control group,hospitalization time of somatostatin group, ulinastatin group,octreotide group and gabexate group were shortened significantly;the incidence of hyperamylasemia and pancre-atitis were significantly decreased;VAS score 3,24,48 h after surgery were significantly decreased,with statistical significance (P<0.05). Compared among 4 groups,above indexes had no significant difference,and the incidence of ADR was in low level and had no statistical significance(P>0.05). The cost-effectiveness ratio of somatostatin group was the lowest and has cost-effec-tiveness advantage. The results were supported by incremental cost-effectiveness and sensitivity analysis. CONCLUSIONS:Soma-tostatin,ulinastatin,octreotide and gabexate can significantly prevent the occurrence of hyperamylasemia and pancreatitis after ER-CP,and relieve pain with good safety. Somatostatin can achieve the best therapeutic efficacy at the lowest cost,so it is the best plan for hyperamylasemia and pancreatitis after ERCP.
10.Evaluation of Bupleuri Radix resources in Qingchuan based on DTOPSIS and grey related degree.
Jie MENG ; Xing-Fu CHEN ; Wen-Yu YANG ; Zhi-Fei LI ; Yu ZHANG ; Jiu-Hua SONG ; Xing-Wang YANG
China Journal of Chinese Materia Medica 2014;39(3):433-437
In order to select high quality and suitable Bupleuri Radix varieties in Qingchuan, and establish a new comprehensive method to evaluation the quality of Bupleuri Radix, 12 characters of 14 samples were evaluated by DTOPSIS and grey related degree. The results showed that varieties No. 8 and No. 10 had high quality. DTOPSIS and grey related degree gave the uniformity result, and the biggest difference of value of Ci in DTOPSIS method was 46. 33% , but the biggest difference of the weighting correlation number( r (i)) in grey related degree was only 13.10%. The DTOPSIS combined with grey related degree can evaluate the quality of Bupleuri Radix comprehensively and objectively.
Bupleurum
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chemistry
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China
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Drugs, Chinese Herbal
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chemistry
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supply & distribution
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Quality Control
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Statistics as Topic
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methods