1.Research advances in molecular epidemiology and vaccines of Coxsackievirus A16.
Xiang-Peng CHEN ; Xiao-Juan TAN ; Wen-Bo XU
Chinese Journal of Virology 2014;30(4):483-488
Epidemics of hand, foot and mouth disease (HFMD) have mainly been caused by Coxsackievirus A16 (CVA16) and Enterovirus A 71 (EV-A71), which circulated alternatively or together in the affected area. CVA16 has caused numerous outbreaks and epidemics in multiple countries and geographical regions, and has become an important public health problem. Based on an analysis of the complete VP1 coding region, all CVA16 strains can be divided into genotypes A, B1, and B2. Furthermore, genotype B1 can be divided into subgenotypes B1a, B1b, and B1c. After 2000, no reports of genotype B2 virus strains have been reported. All of the CVA16 strains reported in mainland China have belonged to subgenotypes B1a and B1b. Most CVA16-associated infections cause only mild symptoms; however, some CVA16 infections can lead to severe complications and even death. Vaccination is considered to be the most effective method to control the transmission and infection rate of this virus. A number of research groups are studying various vaccine types, including inactivated vaccines, genetic engineering vaccines, and DNA vaccines, amongst others. In this review, an overview is provided of the research advances in molecular epidemiology and vaccines of CVA16.
Animals
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China
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Coxsackievirus Infections
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epidemiology
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immunology
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prevention & control
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virology
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Enterovirus A, Human
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classification
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genetics
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isolation & purification
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Humans
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Molecular Epidemiology
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Viral Vaccines
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administration & dosage
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genetics
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immunology
2.EFFECTS OF POLYENE FATTY ACID ON FATTY ACID COMPOSITION, GROWTH AND DEVELOPMENT OF HIPPOCAMPAL NEURONS
Chaogang CHEN ; Yixiang SU ; Bingyan TAN ; Xiang FENG ; Jian WEN ;
Acta Nutrimenta Sinica 1956;0(03):-
Objective: To study the effects of long chain PUFA, AA and/or DHA on the fatty acid composition, growth and development of the newborn rats hippocampal neurons in culture. Methods: The primary culture of the hippocampal neurons was carried out in vitro by using serum free medium and supplementing 4 ? mol/L AA, DHA, or AA and DHA, of which the total concentration were 4 ?mol/L, the ratios of AA∶DHA from 1∶2 to 16∶1, in medium. The hippocampal neuronal fatty acid composition was analyzed by gas chromatogram, and the neuronal size and length of hippocampal neurite were measured by image analysis. Results: There were significant positive correlations between the ratios AA∶DHA in medium and the AA percentage and the ratios AA∶DHA in hippocampal neurons. The soma area? body maximum? minimum diameter and process length of hippocampal neurons in the group, which the total concentration of AA and DHA was 4 ?mol/L, and the ratio AA∶DHA in medium was 2∶1 or 4∶1,were higher significantly than other groups and groups with other ratios of AA∶DHA. Conclusion: AA and DHA could promote the growth and development of hippocampal neurons in culture..
3.The primary study of fluoxetine in the prevention of post-traumatic stress disorder
Xiang JIN ; Qingrong TAN ; Huaning WANG ; Wenming GAO ; Wen WANG ; Hong ZHENG ; Yuanfeng JING
Chinese Journal of Behavioral Medicine and Brain Science 2010;19(5):389-391
Objective To observe the effect of fluoxetine on the single prolonged stress model which mimic the post-traumatic stress disorder (PTSD). Methods Rats receiving single prolonged stress (SPS) (2 h restraint + 20 min FST + anaesthesized to lose consciousness with ethylether) or not were given fluoxetine or tap water for 15 days. Elevated plus maze(EPM),open-field test(OF) and morris water maze(MWM) tests were used to evaluate rats' fear response to environment,high alertness,anxiety & depression behavior,and learning and memory ability. Results In open field test, group of fluoxetine(F1 (8895. 85 ± 599. 78) mm, (40. 23 ±4. 32) s;F2 (8654.07 ±866.05)mm,(41.57 ±4.34)s, P<0.05) showed significant increase in activity times and horizontal motion distance compared with group of SPS (4678.85 ±495.33)mm, (22.15 ±3.43)s, P<0.05). In EPM experiment,group of fluoxetine(F1 (32. 62 ± 4. 57)% , (17. 58 ± 3. 23)% ; F2 (39. 75 ± 4. 46)% , (19. 74 ± 4.44) %) showed significant increase in percentage of the open-arm into the maze and percentage of the open arm pause compared with group of SPS ((23.67 ±2. 87)% ,(12.46 ±2.55)% , P<0.05). In MWM experiment,the escape latency of the SPS group increased significantly in comparison to that in sham group (P<0.01) and fluoxetine group. Fluoxetine significantly reversed the SPS-induced decrease in time spent in the target quadrant (P< 0.05). Conclusion Added fluoxetine can obviously improve rats' fear response to environment ,high alertness ,anxiety & depression behavior as well as learning and memory ability.
4.Determination of plasma concentration of five phenolic acid by LC-MS/MS and study of pharmacokinetics in rats after Mailuoning injection.
Ting WU ; Jun ZHANG ; Heng-Shan TAN ; Wen-Zheng JU ; Xiang-Yang XU
China Journal of Chinese Materia Medica 2014;39(10):1928-1932
To establish a LC-MS/MS method for quantification of chlorogenic acid, caffeic acid, 3,4-DCQA, ferulic acid and cinnamic acid in rats plasma and study its pharmacokinetics after administration of Mailuoning injection at a single dose to rats. Plasma samples were acidified with hydrochloric acid and extracted with ethyl acetate. The analytes were determined by LC-MS-MS using a ZOBAX SB C18 column with a mobile phase of methanol-water (containing 2 mmol x L(-1) ammonium acetic) (60:40)at a flow rate of 0.5 mL x min(-1) and detected using ESI with negative ionization mode. Ions monitored in the multiple reaction monitoring (MRM) mode were m/z 353.1/191.0 [M-H]- for chlorogenic acid, m/z 178.9/134.9 [M-H]- for caffeic acid, m/z 515.2/353.0 [M-H]-for 3,4-DCQA, m/z 193.0/133.9 [M-H]-for ferulic acid, m/z 146.9/102.9 [M-H]- for cinnamic acid and m/z 246.0/125.8 [M-H]- for tinidazole (IS). After administration of Mailuoning injection at a single dose to eight Sprague-Dawley rats, the concentrations of chlorogenic acid, caffeic acid, 3,4-DCQA, ferulic acid and cinnamic acid in plasma were determined by LC-MS/MS method. The main pharmacokinetics parameters of measured data were caluculated by using DASver 1.0 software. The linear concentration ranges of the calibration curves for chlorogenic acid, caffeic acid, 3,4-DCQA and cinnamic acid were 2.006-1,027 microg x L(-1) (r = 0.999 6), 1.953-1,000 microg x L(-1) (r = 0.999 7), 28.51-1.459 x 10(4) microg x L(-1) (r = 0.998 9), 1.836-940.0, g x L(-1) (r = 0.997 7) and 4.780-2,447 microg x L(-1) (r = 0.998 6) respectively. The inner and inter-days relative standard deviations were both less than 5.0%, indicating legitimate precise and accuracy to the requirement of biological sample analysis. For chlorogenic acid, the pharmacokinetic parameter t1/2, AUC0-t, and CL were (49.78 +/- 12.81) min, (123.55 +/- 14.82) mg x min x L(-1) and (0.004 3 +/- 0.000 5) L x min(-1), respectively. For caffeic acid, the pharmacokinetic parameter t1/2, AUC0-t, and CL were (36.65 +/- 10.59) min, (91.67 +/- 11.77) mg x min L(-1) and (0.005 7 +/- 0.000 7) L x min(-1), respectively. For 3,4-DCQA, the pharmacokinetic parameter t1/2, AUC0-t, and CL were (50.08 +/- 13.78) min, (278.34 +/- 31.82) mg x min x L-1 and (0.001 6 +/- 0.000 2) L x min(-1), respectively. For ferulic acid, the pharmacokinetic parameter t1/2, AUC0-t, and CL were (51.39 +/- 15.52) min, (34.72 +/- 4.67) mg x min x L(-1) and (0.000 4 +/- 0.0001) L x min(-1), respectively. For cinnamic acid, the pharmacokinetic parameter t1/2, AUCo-t, and CL were (74.42 +/- 18.32) min, (34.63 +/- 4.82) mg x min x L(-1) and (0.007 7 +/- 0.001 1) L x min-', respectively. The assay method is proved to be sensitive, accurate and convenient. It can be applied to the pharmacokinetic study of chlorogenic acid, caffeic acid, 3,4-DCQA, ferulic acid and cinnamic acid.
Animals
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Chromatography, Liquid
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methods
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Drugs, Chinese Herbal
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administration & dosage
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pharmacokinetics
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Female
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Hydroxybenzoates
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blood
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pharmacokinetics
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Male
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Rats
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Rats, Sprague-Dawley
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Tandem Mass Spectrometry
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methods
5.Immunoprotective effect of inactivated coxsackievirus A16 vaccine in mice.
Xiang-Peng CHEN ; Xiao-Juan TAN ; Yong ZHANG ; Wen-Bo XU
Chinese Journal of Virology 2014;30(3):226-232
This study aims to construct inactivated coxsackievirus A16 (CVA16) vaccine and to investigate its protective effect in ICR mice. A clinical isolate of CVA16, 521-01T, was cultured in VERO cells, inactivated by formaldehyde, and purified by ultracentrifugation for vaccine preparation. Purity and other characteristics of the vaccine were determined by SDS-PAGE and Western blot. Female ICR mice were subcutaneously inoculated with inactivated CVA16 or Al(OH)3-absorbed CVA16, followed by booster immunization at the end of 2 and 4 weeks. CVA16-specific IgG titers in serum were determined by ELISA, and titers of neutralizing antibodies were determined by viral neutralization assay. The immunity of T lymphocytes was evaluated by IFN-gamma ELISPOT assay. The protective effect was evaluated by challenging the neonatal offspring (< 48 hours) of vaccinated female mice with 1 000 LD50 of CVA16 521-01T. The mortality rates of different groups were compared. The results showed that Al(OH)3 +CVA16 could induce high titers of specific IgG antibodies in ICR mice. After being boosted two times, the serum IgG antibody titer could reach up to 1 : 1 x 10(5) (P = 0.000), and neutralizing antibody titer was higher than 1 : 256. Additionally, more spot forming cells were induced in the immunized groups than in the negative controls. The maternal antibodies showed protective effect in 100% of the neonatal mice challenged with 1 000 LD50 of CVA16 521-01T. The inactivated CVA16 vaccine has ideal immunogenicity and immunoprotective effect. This research lays a foundation for the development and evaluation of CVA16 vaccines.
Animals
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Antibodies, Neutralizing
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immunology
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Antibodies, Viral
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immunology
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Enterovirus
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immunology
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Enterovirus Infections
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immunology
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prevention & control
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virology
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Female
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Humans
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Immunization
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Mice
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Mice, Inbred ICR
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T-Lymphocytes
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immunology
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virology
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Vaccines, Inactivated
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administration & dosage
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immunology
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Viral Vaccines
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administration & dosage
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immunology
6.Establishment of perfusion technique for isolated rat pancreas
Nai-Qian ZHAO ; Ye-Rong YU ; Hui-Wen TAN ; Zhi-Ming LU ; Xiang-Xun ZHANG ; Jun-Jie LI ;
Chinese Journal of Endocrinology and Metabolism 2001;0(05):-
Objective To establish an isolated rat pancreas perfusion technique,a method for the precise measurement of insulin secretion in vitro.Methods An isolated rat pancreas perfusion technique was applied in the study of insulin secretion from?-cells in 10 high-fat diet-induced obese Wistar rats.Results For the assessment of the functional integrity of the perfused pancreas,the isolated pancreas of 6 rats met all the criteria: (1)The constancy of perfusion pressure was kept over the whole experiment time[(70?5)mm Hg,1 mm Hg= 0.133 kPa].(2)The duodenal peristaltic activity of isolated pancreas and duodenum block was present after perfusion experiment.(3)Total insulin response to arginine stimulation was significantly increased as compared with glucose stimulation[maximum insulin secretion rate:(987?100)?U/min vs(545?50)?U/min,P
7.Study on three different species tibetan medicine sea buckthorn by 1H-NMR-based metabonomics.
Yong-Wen SU ; Er TAN ; Jing ZHANG ; Jia-Li YOU ; Yue LIU ; Chuan LIU ; Xiang-Dong ZHOU ; Yi ZHANG
China Journal of Chinese Materia Medica 2014;39(21):4234-4239
The 1H-NMR fingerprints of three different species tibetan medicine sea buckthorn were established by 1H-HMR metabolomics to find out different motablism which could provide a new method for the quality evaluation of sea buckthorn. The obtained free induction decay (FID) signal will be imported into MestReNova software and into divide segments. The data will be normalized and processed by principal component analysis and.partial least squares discriminant analysis to perform pattern recognition. The results showed that 25 metabolites belonging to different chemical types were detected from sea buckthorn,including flavonoids, triterpenoids, amino acids, carbohydrates, fatty acids, etc. PCA and PLS-DA analysis showed three different varietiest of sea buckthorn that can be clearly separated by the content of L-quebrachitol, malic acid and some unidentified sugars, which can be used as the differences metabolites of three species of sea buckthorn. 1H-NMR-based metabonomies method had a holistic characteristic with sample preparation and handling. The results of this study can offer an important reference for the species identification and quality control of sea buckthorn.
Hippophae
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metabolism
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Magnetic Resonance Spectroscopy
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methods
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Medicine, Tibetan Traditional
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Metabolomics
8.The preparation of an elicitor from a fungal endophyte to enhance artemisinin production in hairy root cultures of Artemisia annua L.
Jian-wen WANG ; Li-Ping ZHENG ; Ren-Xiang TAN
Chinese Journal of Biotechnology 2006;22(5):829-834
The different components of crude mycelium of the predominant endophytic Colletotrichum gloeosporioides of Artemisa annua have been extracted by the methods of acid hydrolysate. We compared the effect of the isolated components on artemisin biosynthesis in hairy root cultures. Therefore, the oligosaccharide elicitor from C. gloeosporioides has been partially purified by column chromatography of Sephadex G25. The isolated oligosaccharide B II (elicitor, MW < 2500) has been revealed to promote the production of artemisinin in Artemisia annua hairy root cultures. When hairy roots of 23-day old cultures (later growth phase) were exposed to the elicitor at 0.4 mg/mL for 4 days, the maximum production of artemisinin reached to 13.51 mg/L, a 51.63% increase over the control. This is the first report on the stimulation of artemisinin production in hairy roots by the oligosaccharide elicitor from an endophytic fungus of A. annua.
Artemisia annua
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drug effects
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growth & development
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metabolism
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Artemisinins
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metabolism
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Bioreactors
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Colletotrichum
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chemistry
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Mycelium
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chemistry
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Oligosaccharides
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isolation & purification
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pharmacology
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Plant Roots
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drug effects
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growth & development
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metabolism
9.Visualization of the adult penis and adjacent structure of the Chinese visible human.
Hua CHEN ; Shi-rong LI ; Shao-xiang ZHANG ; Li-wen TAN
Chinese Journal of Plastic Surgery 2006;22(6):458-461
OBJECTIVETo build digitized visible model of the adult penis and adjacent structure of the Chinese visible human, providing morphological data for plastic plerosis of external genitals diagnosis and the adult penis surgery planning.
METHODSCross-sectional images of fresh tissues from the Chinese visible human dataset and the visible penis and adjacent structure dataset were reviewed, the structures of the penis and adjacent structure were confirmed on a section-by-section basis. Three-dimensional computer reconstructions of the penis and adjacent structure were generated from these data by surface rendering.
RESULTSThe quality of the computerized 3D-reconstructed images was distinct and perfect. The adult penis and adjacent structure were all reconstructed and displayed jointly. All reconstructed structures can be represented individually or jointly. Any diameter and angle of the structures reconstructed could be measured conveniently.
CONCLUSIONSThe digitized model of the penis and adjacent structure offer unique insights into the complex penis anatomy, providing morphological data for plastic plerosis of external genitals diagnosis and surgery planning.
Adult ; Asian Continental Ancestry Group ; Genitalia, Male ; anatomy & histology ; Humans ; Imaging, Three-Dimensional ; methods ; Male ; Models, Anatomic ; Penis ; anatomy & histology ; Software ; Visible Human Projects
10.Preparation of the anti-HLJ1 monoclonal antibodies and establishment of method for detection of the antigen.
Xiang LIN ; Li MA ; Jufang WANG ; Yongfa TAN ; Qian WEN ; Wei LUO ; Jin SU ; Ying LIN ; Xiaoning WANG
Chinese Journal of Biotechnology 2008;24(7):1293-1299
Monoclonal antibodies (McAbs) against human liver DnaJ-like protein (HLJ1) was produced by using lymphocyte-hybridoma technique and then one method for the detection of HLJ1 antigen was established. Two hybridoma cell lines which stably secreted monoclonal antibodies against HLJ1 were generated and named for A4C7 and C4C8. Subtypes of the two McAbs were both IgG1, and the antibodies showed high titer and good specificity. Using the prepared monoclonal antibody, human embryonic liver tissues were examined by immunohistochemistry. The results indicated that HLJ1 located in the cytoplasm of the human embryonic liver cell. A double antibodies sandwich ELISA was established by using C4C8 and HRP labeled A4C7. This assay had good specificity, and the lowest detection limit was 7.5 ng/mL and the linear range was 7.5-750 ng/mL. In conclusion, an immunohistochemistry method and a sensitive sandwich ELISA were established for the detection of HLJ1 protein.
Animals
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Antibodies, Monoclonal
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biosynthesis
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Enzyme-Linked Immunosorbent Assay
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methods
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Female
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HSP40 Heat-Shock Proteins
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blood
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immunology
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Humans
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Hybridomas
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secretion
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Immunohistochemistry
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Mice
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Mice, Inbred BALB C