This paper is aimed to promote the learning interest and motivation of students and improve the teaching effect, then the interactive courseware of analysis of abnormal gait is done. A courseware should emphasize particularly on optic analysis of abnormal gait, which is closely related to clinical medicine. The interactive courseware was organized with Office 2000, Photo shop 7.0, 3D Studio MAX 3.0 and Hero Super Player 6.5, in which several materials were included, such as the video kinescope of abnormal gaits, clinical features, analysis of mechanics, etiologic analysis and rehabilitation measures of abnormal gaits. There are some pushbuttons in the courseware to achieve controls, such as play, pause, speed, recoil and return end. A majority of students believe that their learning interests of the abnormal gait analysis are improved and have a perceptible idea about abnormal gait that is abstract and vague.

Objective To investigate possible nerological origination of rabbit P3a potential. Method we simultaneously recorded the ERPs at dura and various depths beneath the skull in a point of rabbit skull, similarly did some in different points of rabbit skull. Result In the Brodmann 25 and 32 areas(AcgI), the polarity of P3a to those at dura was markedly reverse and the reverse rate of P3a(81.3%) was significantly higher than other locations, the P3a without polarity reversal showed steep voltage gradient. There was an increasing trend of the P3a reversal and moderate voltage gradient nearby the AcgI, but not other regions. Conclusion Rabbit P3a potential possibly originate from the AcgI acea.

Objective To investigate the generative and regulatory effects of AchMR on P 3a and its mechanism in different subregions of rabbit cingulate gyrus. Methods Event related potentials(ERPs) P 3a potentials in Normal, AcgI, AcgⅡand Pcg regions were recorded after microinfusion of 0.5 mmol/L scopolamine at three different time points(instantly, at the 15th minute and at the 30th minute), 2 mmol/L acetylcholine and 1 mmol/L scopolamine. Results The P 3a amplitude decreased and/or P 3a latency increased with scopolamine in time and dose dependent pattern in AcgⅠ, AcgⅡ and Pcg, and P 3a might disappear only in AcgⅠ and the P 3a again be recorded after microinfusion of acetylcholine in AcgⅠ. Conclusion The action of AchMR in AcgⅠ area markedly affects the generation and/or regulation of P 3a and confirms the AcgⅠ is the source of rabbit P 3a potential. The action of neurons in AcgⅠ might facilitate the P 3a potential.

Pancreatic cancer is a commonly malignant gastrointestinal tumor with an obviously increasing incidence all over the world.Those patients without specific symptoms at early stage had mostly lost the opportunity of surgical therapy when pancreatic cancer was detected at advanced stage,and their prognosis is poor.Therefore,it is rather crucial to improve the early diagnosis of pancreatic cancer.Tumor marker is a considerable tool for early tumor detection and screening.It will help to improve the diagnostic rate of early pancreatic cancer and promote the prognosis of pancreatic cancer if we could find out tumor markers and screen one or a group of pancreatic cancer tumor markers.

Objective To analyze the relationship between renal function impairement and obesity in patients with primary hypertension.Methods One hundred and twenty-eight primary hypertension patients with renal function impairement admitted to our hospital from January 2012 to December 2015 were divided into obesity group (n=68) and control group (n=60) according to the Asian obesity diagnosis criteria established by the International Diabetes Federation.Relationship of serum urea nitrogen (BUN),creatinine,cystatin C (Cys-C),urinary N-acetyl beta-D glucosaminidase (NAG),urinary micro albumin levels and BMI with renal function impairment in primary hypertension patients was analyzed by multivariate linear stepwise regression analysis.Results The BMI,serum levels of urinary NAG,Cys-C,BUN,and creatinine were significantly higher in obesity group than in control group (31.70±1.80 kg/m2 vs 23.95±2.00 kg/m2,19.34±1.40 U/L vs 14.92±1.31 U/L,0.69±0.06 mg/L vs 0.47±0.05 mg/L,11.50±2.90 mmol/L vs 8.60±1.90 mmol/L,159.50±10.40 μmol/L vs 148.90±10.70 μmol/L,P＜0.05).The incidence of urinary micro albumin was significantly higher in obesity group than in control group (79.4％ vs 46.7 ％,P＜ 0.01).Blood pressure,pulse pressure difference,BMI,fasting blood glucose,serum levels of ereatinine,urinary NAG,Cys-C,BUN and urinary micro albumin were the risk factors for renal function impairement in primary hypertension patients (P＜0.05,P＜0.01).Conclusion Renal function impairement is related with obesity in primary hypertension patients.

Antigen challenge of sensitized isolated guinea-pig mesentery perfused under constant pressure resulted in a significant flow reduction which was induced by the release of leukotrienes bioassayed on guinea-pig ileum. 3 min after challenge, the mescnteric perfusion rate dropped to a minimum of 32?12% ( P

Objective To increase the appreciation of manifestations in autoimmune pancreatitis (AIP) and to decrease misdiagnosis rate by investigating the clinical characteristics of AIP. Methods Clinical data were collected and laboratory ,imaging and histopathology were analyzed from the Chinese PLA General Hospital from 1995 to 2009. Patients with AIP were included in the study. Results Eight patients (male 6, female 2) aged 35-69 (52.4 ±9.4) years were diagnosed as AIP from 2006 to 2009. The main clinical manifestations include intermittent jaundice in 6 cases (6/8), abdominal pain in 5 cases (5/8),weight loss in 4 cases (4/8), and accompanied with other diseases of immune system in 4 cases (4/8).The imaging showed head of pancreas enlargement in 3 cases (3/8) and whole pancreas enlargement in 5 cases (5/8). There is a "banana-peel like" ring around the pancreas and irregular stenosis of pancreatic duct. Massive lymphocytes and plasma cells infiltration and parenchymal fibrosis were shown in pancreatic tissues, bile duct,salivary gland and liver in pathology. Clinical manifestations, laboratory examinations and images in 7 cases (7/8) were improved after treatment with prednisone. Twenty-two of them were misdiagnosed as pancreatic or biliary carcinoma and 21 were performed laparotomy in the period of 1995-2005 ( 23 cases). After 2006, however, the misdiagnosis rate significantly decreased from 95.7% ( 22/23 ) to 0. Conclusions AIP seems to be a systemic autoimmune disease rather than an isolated disorder,markedly overlapping with other autoimmune diseases. Definitive diagnosis can be improved by the detection of immune parameters and pathological examination.

Objective To explore the role of Th17/IL-17 in the pathogenesis of asthma in children.Methods Thirty asthma children (asthma group) and 20 healthy volunteers (control group) were selected as our subjects.Percentage of Th17 cell in peripheral blood mononuclear cell (PBMC) of asthma children was detected by flow cytometry,the level of IL-17 and IgE in plasma were examined by enzyme linked immunosorbent assay.Results Proportion of Th17 cells in PBMC of children in asthma group was (1.83 ± 1.01) ％,significantly higher than that in control group ((1.02 ± 0.49) ％ ; t =3.896 ; P ＜ 0.01).The IL-17 level in control group was (19.11 ± 3.23) ng/L,significant lower than that in asthma group ((34.23 ± 4.88) ng/L;t =6.261 ;P ＜ 0.01)).The Ig E level in asthma group was (399.4 ± 45.1) ng/L,significantly higher than in control group((58.2 ± 19.7) ng/L;t =7.244,P ＜0.01).The level of IL-17 in plasma had a postive relationship with the proportion of Th17 cells(r =0.882,P ＜0.01),but not relationship was seen with the level of IgE (r =0.375,P ＜ 0.05).Conclusion Th1 7 cell is associated with asthma development of children,and the reason may due to probably aggravating asthma condition through increasing inflammation secreting IL-17.

Objective To discuss the CT features of mediastinal teratoma.Methods 35 patients with mediastinal teratoma confirmed by clinical and pathologic data were retrospectively analyzed.Results Most mediastinal teratomas(97.1%) were localized in the middle or upper area of anterior mediastinum.Mediastinal teratoma included cystic teratoma(60.0%) and solid teratoma(40.0%).All kinds of tissues could be found in the tumors.CT features of cystic teratoma(n=21) were the cystic mass with fluid density,most of them(n=19) had the characteristics of "obviouswalled cysts".CT features of solid teratoma(n=14) were the soft-tissue mass with mixed densities.CT features of malignant teratoma(n=5) were that the layer of fatty tissue between the tumor and the surrounding structures disappeared,and that the wall of neighbour vessels were mostly or all surrounded by the tumor.Mediastinal teratoma could cause pleural offusion(n=6) and pericardial offusion(n=12).Conclusion Mediastinal teratomas have obvious CT imaging features.CT has very important value in the diagnosis and differential diagnosis of mediastinal teratoma.

Berberine is a taste of traditional Chinese medicine.With the progress of research on pharmacology and mechanism of drug action of extensive research,the new mechanism of berberine was revealed through a series of clinical trials,the clinical indications continue to expand,and berberine has a therapeutic effect in digestive and endocrine system disease.The current study confirmed that berberine not only has the broad-spectrum antibacterial activity,but also plays a strong anti-inflammatory and immune regulatory role,which makes more and more research began to concern its application value in the diseases of respiratory system.The strong anti-inflammatory effect of berberine cau effectively relieve the airway inflammation of patients who are suffering from chronic obstructive pulmonary,and the broad-spectrum antibacterial effect brought hope for the treatment of tuberculosis and pneumonia.The subsequent animal and clinical studies have confirmed that berberine can effectively improve the symptoms of respiratory system diseases,and play a role in the treatment.