Along with the popularization and application of the computer, masses of information could be achieved from the internet, ensuring the well operation of internet is becoming the prior issue. The paper introduces some successful experiences in ensuring the well operation of internet, including broadening the broadband communications, adding extra network firewall, purchasing products for network safety management, installing anti -virus software, regulating the access to the internet, etc.

China ; Humans ; Hypertension ; diagnosis ; epidemiology ; therapy

Cl~- localization and periodic acid-silver methenamine techniques have been used for ultrastructural studies on parietal cells of mouse stomach. Cl~- is located only in the intracellular canaliculi (IC) and intercellular space (IS)of parietal cells. The tubulovesicular system (TS) shows various reactions in periodic acid-silver methenamine staining. The positive reaction was demonstrated in the TS which is located near the apical membranes. This indicates that acid secretion is accompanied by polysaccharides secretion in which the TS takes part, and the polysaccharides secretory activity shows its cyclicity which is characterized by the changes of amounts of secretion. The cycle of parietal cells do not synchronize.

Though implementing the quality management system of ISO9000 series standard in the hospital,it enables to make the nursing quality in a controlled state and make every service and operation of nursing staff have regulations to abide by.According to ISO9000 series standard,implementing the management of“procedureization”makes the nursing work closed to the need of patients and satisfies them by stipulating each workflow clearly.

OBJECTIVE: To evaluate the anti-thrombus activitity of EPR-hirudin (EH), a derivative of hirudin obtained by adding three amino acids at the nitrogen terminus. METHODS: The anti-thrombus activitity of EH was investigated by chromophore substrate method in vitro as well as by rat models of carotid arterial thrombosis and inferior vena cava thrombosis in vivo. RESULTS: The result of anti-thrombin experiment in vitro showed that EH did not produced anti-thrombin activity unless it was cleaved by blood coagulation factors. Then the anti-thrombus feature of EH was confirmed in vivo separately in the rat models of carotid arterial thrombosis and inferior vena cava thrombosis. The formation of arterial clot was inhibited and the weight of venous clot was decreased after administration of EH in a dose-independent manner. However, the blooding time, activated partial thromboplastin time (APTT) and thrombin time (TT) did not significantly increase in EH groups compared with HV and low molecular heparin. CONCLUSION: EPR-hirudin can inhibit the formation of arterial and venous clot and has low risk of blooding.

Biomarkers ; Carcinogens ; Epigenesis, Genetic ; Humans

Child, Preschool ; Embolism ; complications ; physiopathology ; Humans ; Male ; Mediastinal Emphysema ; etiology ; Pulmonary Atelectasis ; etiology ; Sputum ; physiology ; Subcutaneous Emphysema ; etiology

Berberine is a taste of traditional Chinese medicine.With the progress of research on pharmacology and mechanism of drug action of extensive research,the new mechanism of berberine was revealed through a series of clinical trials,the clinical indications continue to expand,and berberine has a therapeutic effect in digestive and endocrine system disease.The current study confirmed that berberine not only has the broad-spectrum antibacterial activity,but also plays a strong anti-inflammatory and immune regulatory role,which makes more and more research began to concern its application value in the diseases of respiratory system.The strong anti-inflammatory effect of berberine cau effectively relieve the airway inflammation of patients who are suffering from chronic obstructive pulmonary,and the broad-spectrum antibacterial effect brought hope for the treatment of tuberculosis and pneumonia.The subsequent animal and clinical studies have confirmed that berberine can effectively improve the symptoms of respiratory system diseases,and play a role in the treatment.

Objective:To enhance the immune efficiency of porcine reproductive and respiratory syndrome virus(PRRSV)DNA vaccine,the ORF5 genes were used as candidate genes to construct the recombinant plasmids CpG-pVAX1-ORF5 by pVAX1 as eukaryotic expression vector.The aim is to analyze the immune responses and the effects of CpG-ODN induced by GP5 recombinant plasmids of PRRSV.Methods:Piglets were immunized with recombinant DNA plasmids which expressed PRRSV GP5 for three inoculations.The PRRSV antibody in serum,the concentration of IL-2 and the lymphocyte proliferation test(MTT) in peripheral blood of vaccinated piglets were detected.The vaccinated pigs were challenged intranasally with PRRSV SD2.Results:GP5 DNA immunization with CpG resulted in the production of both PRRSV antibodies and cellular immune(a significant enhancement of a lymphoproliferative response ).The CpG-pVAX1-ORF5 was showed significantly better protection from the PRRSV challenge compared with the control plasmid.This immune response was characterized by a significantly decreased frequency of viraemia with decrease of 80% compared with the control group.There were little clinical symptoms and protection in some extent from lung damage.Conclusion:The results indicate that CpG-ODN could be used as immune adjuvant of PRRSV DNA vaccine.

A total of 96 26-week-old Hisex Bro-wn laying hens were divided into 3 groups (32 each) to investigate the effect of niacin on egg production and lipid metabolism of laying hens. Three levels of dietary niacin were used (0, 20 and 60mg/kg diet) for the 8-week experimental period.Supplementation of dietary niacin caused plasma cholesterol, ?-lipoprotein, triglycerides and free fatty acid to decrease 40.9%, 35.7%, 18.2% and 54.3% respectively and liver fat, muscle fat and abdominal fat to decrease 26.7%, 33.4% and 12.3%, respectively, compared with control. Increasing of dietary niacin resulted in a significantly decreases in plasma estradiol (E2) and the activity of hepatic malate dehydrogenase (MD) to regulate lipid matabolism.All of the results demonstrated that niacin could inhibit the lipid synthesis in hen's liver. Niacin deficiency could caused hen liver fragile and yellow in color, liver cells damaged and further fatty liver syndrome in hens.It is suggested that the supplementation of 20mg niacin/kg of diet is appropriate to laying hens if the basal diet contains 0.20% tryptophan and 23.8mg/kg diet of niacin originally.