Objective To explore the diagnostic value of carcino embryonic antigen (CEA),squamous cell carcinoma antigen(SCC),human papilloma virus-E7 (HPV-E7) in cervical carcinoma.Methods A total of 107 cases of women patients treated in hospital from July 2013 to July 2015 accorded to the pathological examination results were divided into cervical cancer group 60 cases and CIN group 47 cases,another 50 cases of healthy people were selected as control group, and serum expression levels of HPV-E7, CEA and SCC in the three groups were detected by enzyme-linked immunosorbent assay.Results The serum HPV-E7, CEA and SCC in cervical cancer group were significantly higher than those in CIN group and control group (P<0.05).There was no significant difference in serum HPV-E7, CEA and SCC between CIN group and control group.The levels of serum HPV-E7, CEA and SCC in stage I-II were significantly lower than those of stage III-IV in patients with cervical cancer, and the difference with statistically significant between two groups(P<0.05).The area under the ROC of HPV-E7 was significantly higher than that of CEA and SCC (Z=2.914,2.951, P<0.05), and there was no significant difference in the area under the ROC between CEA and SCC (Z=1.580,P=0.057).Conclusion The serum HPV-E7, CEA and SCC in cervical cancer patients are significantly higher, the diagnosis value of HPV-E7 is higher which is expected to become one of the effective indicators of cervical cancer diagnosis.

Objective To study the prevalence of chronic kidney disease(CKD) and risk factors among adults received examina-tion in Yongchuan district of Chongqing .Methods 10 280 residents(older than 18 years) were selected ,who had health examina-tion in the Yongchuan Affiliated Hospital ,Chongqing Medical University ,Yongchuan Hospital of Traditional Chinese Medicine and Yongchuan people's hospital from January 2012 to December 2013 .Patients with CKD were identified by the examination dates .All of them were conducted with a questionnaire survey ,physical examination ,and renal damage and related risk indicator factors moni-toring for more than 3 months .Results Eligible dates of 10 021 subjects were enrolled in the study .The prevalence of albuminuria was 5 .1% ,hematuria in 8 .3% ,reduced renal function in 1 .4% .The CKD population prevalence rate was 15 .7% ,and the recogni-tion was 6 .4% .The Logistic regression model showed that hyperuricemia ,nephrolithiasis ,diabetes mellitus ,hypertension ,obesity and age contributed to the development of CKD .Conclusion The prevalence of chronic kidney disease is 15 .7% and the recognition is 6 .4% in urban healthy examination population of Yongchuan .Independent risk factors associated with CKD are hyperuricemia , nephrolithiasis ,diabetes mellitus ,hypertension ,obesity and age .

Objective To investigate characteristics of emotional intelligence(EI)and the ways of occupational stress coping in nurses as well as the predictive validity of EI on coping strategies.Methods 464 nurses from general hospitals were collected and evaluated with psychometric instruments including:Wong's Emotional Intelligence Scale(WEIS)and Nurse Ways of Coping Questionnaire.Data were conducted by Logistic regress analysis according to key themes.Results (1)Nurses' demographic characteristics had effects to the ways of occupational stress coping and emotional intelligence.However,there was no statistical difference of El varied with education levels(P＞0.05).(2)Both problems solving and positive recognition are main coping styles of nurses towards occupational stress.Either avoidance or self-reproach was seldom coping strategies of nurses toward occupational pressures(2.60±0.55;2.60±0.75;1.37±0.69;respectively).(3)El was correlated with coping styles(r=-0.123～0.253,P＜0.05,P＜0.01).Conclusion Four dimensions of EI positively or negatively predicted six kinds of occupational stress coping strategies separately.

Female ; Hepatitis C ; complications ; drug therapy ; Humans ; Interferon-alpha ; therapeutic use ; Interferons ; therapeutic use ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; complications ; drug therapy ; virology ; Middle Aged ; Polyethylene Glycols ; therapeutic use ; Recombinant Proteins ; therapeutic use ; Treatment Outcome

Thrombolytic therapy is a safe and effective way to cure thrombosis. Developing the safe,effective and cheap therapeutic thromolytic agents are important to prevent and cure thrombosis. In recent years,several fibrinolytic enzymes have been found in the resources of Asian traditional fermented foods,such as Japanese natto,Korea Chungkook-Jang,Chinese Douchi and fermented shrimp paste. These fibrinolytic enzymes secreted by food-grade microorganisms are safe and effective,and are potent thrombolytic agents. The physical and biochemical properties,thrombolytic characteristics,and the potential applications of these fibrinolytic enzymes from Asian fermented food,are reviewed.

Ear Diseases ; complications ; Ear, Inner ; Female ; Hearing Loss, Sudden ; etiology ; Hemorrhage ; complications ; Humans ; Middle Aged

OBJECTIVE:To compare the therapeutic effects of8-methoxypsoralen(8-MOP)liposomal gel with other dosage forms of8-MOP in equal concentration on guinea-pig models of vitiligo.METHODS:The guinea-pigs were divided into normal control,model,8-MOP tincture,8-MOP gel and8-MOP liposome gel groups.The vitiligo model was established by chemical decoloration method in all groups except normal control group,then corresponding preparations were topically ap?plied.The therapeutic effects were compared and the melanogenesis of hair follicles,serum acetylcholinesterases and skin MDA contents were observed in all groups.RESULTS:8-MOP liposomal gel had better therapeutic effects on the animal models,and could improve the dermatic melanogenesis and acetylcholinesterase of the animal model,and also had effect on MDA of skin.The differences between8-MOP liposomal gel group and model group were highly significant(P

OBJECTIVE:To prepare psoralen liposome gel and to conduct a quan titative investigation about its drug re?lease model in vitro.METHODS:Taken psoralen liposome gel that of the same concentration as the control group,the model of drug release in vitro of the testing group was evaluated by dialyzing method and the stability of its drug release after storage for3weeks at4℃was studied as well.RESULTS:Compared with the control group,the testing group showed significant slow-releasing and long-acting effects and the drug release followed the Higuchi(k=4.67%h -1/2 )diffusion model in the first3hours and a zero order drug release model(k=0.74%h -1 )3hours later;The drug release of the control group followed the Higuchi(k=7.18%h -1/2 )diffusion model of within24hours;The drug release model and the envelop rates of the testing group remained stable within the storage date.CONCLUSION:This preparation is characterized by slow drug releasing in vitro and good stability.

OBJECTIVE:To prepare lidocaine liposome gel and to establish its quality control method.METHODS:Li-docaine liposome was prepared using a film dispersion method and the liposome gels were prepared with carbomer-940as base.The concentration of lidocaine was determined by HPLC and the lipsome entrapment efficiency was determined by centrifuga-tion.RESULTS:The linear detection concentration range of lidocaine was2.5～25.0mg/ml(r=0.9996)with average re-covery at(99.83?0.53)%and entrapment efficiency at(82.7?1.83)%.CONCLUSION:This liposome gel is feasible in preparation techniques,stable in quality,convenient and reliable in quality control method.