1.A case report of sudden death caused by carcinoma of nasal sinuses with a huge liquid mass in cranial cavity.
Jia-xiong ZHANG ; Wen-tao ZOU ; Xiao-jing CAI
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2012;47(11):948-948
Adult
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Brain Neoplasms
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complications
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Death, Sudden
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etiology
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Female
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Humans
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Nose Neoplasms
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complications
2.Optimum harvest study of Gentiana crassicaulis in Ludian.
Yu ZENG ; Xing-Fu CHEN ; Yuan-Feng ZOU ; Jiu-Hua SONG ; Wen-Yu YANG ; Tao CHENG
China Journal of Chinese Materia Medica 2014;39(14):2635-2639
The paper is aimed to study the difference in yield and quality at different harvest time and determine the optimum harvest of planting Gentiana in Ludian traditional harvest period. The authors analyzed the variation in fresh weight, dry weight, dry discount rate, length, diameter, volume and the content of gentiopicroside, loganin acid, alcohol-soluble extract and total ash and made a comprehensive appraisal of yield, appearance quality and intrinsic quality by gray relational distance ideal Comprehensive Evaluation method. The results showed that there is a big difference in yield and quality both 2-year-old and 3-year-old Gentiana harvested in traditional harvest period and the comprehensive evaluation more better when harvested more later. It can be seen, Gentiana harvested the later had a better yield and quality in Ludian traditional harvest period. The harvest of Gentiana can be appropriate delayed depending on the particular circumstances of production.
Agriculture
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methods
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China
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Gentiana
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anatomy & histology
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growth & development
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metabolism
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Iridoid Glucosides
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metabolism
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Organ Size
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Quality Control
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Time Factors
3.Impacts of acupuncture on blood pressure and hematoma in patients of cerebral hemorrhage at the early stage.
Wen-Qiang TAO ; Hai-Yun FANG ; Zuo-Qiang ZOU ; Yi LUO ; Yin-Feng LI
Chinese Acupuncture & Moxibustion 2014;34(5):426-430
OBJECTIVETo explore the therapeutic effect of acupuncture for hypertensive cerebral hemorrhage at the early stage.
METHODSFifty-four cases of small-amount cerebral hemorrhage were randomized into an acupuncture group and a conventional treatment group, 27 cases in each one. In the conventional treatment group, special care, oxygen therapy, nerve nutrition and symptomatic support were applied. In necessary, dehydrant and hypotensive drugs were prescribed for antihypertension, or surgery was given. In the acupuncture group, on the basis of the treatment as the control group, acupuncture was applied at Quchi (LI 11), Neiguan (PC 6), Zusanli (ST 36), Sanyinjiao (SP 6) and Taichong (LR 3). Acupuncture was given at the admission, 4 h, 6 h and 12 h after disease onset respectively. Blood pressure was monitored in the whole procedure. 6 h and 24 h after disease onset, the cranial CT was re-examined. Systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP), hematoma volume and neurological deficit score were compared at different time points between the two groups.
RESULTS(1) Blood pressure: from the admission to 12 h after disease onset, SBP, DBP and MAP were increased apparently in the conventional treatment group and increased slightly in the acupuncture group. The differences in SBP [(164.3 +/- 21.6) mmHg vs (158.6 +/- 21.5) mmHg] and MAP [(113.4 +/- 4.9) mmHg vs (106.7 +/- 6.1) mmHg] were significant between the two groups (both P < 0.05). From 12 h to 24 h after disease onset, compared with the conventional treatment group, SBP and MAP were decreased apparently in the acupuncture group [(147.3 +/- 21.6) mmHg vs (158.4 +/- 23.5) mmHg, (97.2 +/- 5.3) mmHg vs (106.6 +/- 5.1) mmHg, both P < 0.05)]. (2) Hematoma volume: from the admission to 6 h after disease onset, the volume was increased by (4.15 +/- 0.73) mL in the convertional treatment group and (2.67 +/- 0.33) mL in the acupuncture group, indicating the significant difference in comparison (P < 0.05). From the admission to 24 h after disease onset, it was increased by (5.57 +/- 1.26) mL in the convertional treatment group and (3.14 +/- 1.18) mL in the acupuncture group, indicating the significant difference in comparison (P < 0.05). (3) Neurological deficit score: the score was increasing gradually in first 3 days after disease onset in the two groups. The score (38.39 +/- 6.84) in the acupuncture group on the first day was different significantly as compared with that (42.37 +/- 7.46) in the conventional treatment group (P < 0.05). On the 10th days, the score (24.68 +/- 5.42) in the acupuncture group was different significantly from that (29.74 +/- 7.36) in the convertional treatment group (P < 0.05).
CONCLUSIONThere is no peak of blood pressure rising, and the continuous hemorrhagic volume is less in 24 h and neurological deficit score is improved in the acupuncture group. Acupuncture brings the positive significance in the treatment of cerebral hemorrhage at the early stage.
Acupuncture Therapy ; Adult ; Aged ; Blood Pressure ; Cerebral Hemorrhage ; physiopathology ; therapy ; Female ; Hematoma ; physiopathology ; therapy ; Humans ; Male ; Middle Aged
4.Expressions of Protease Activated Receptors on Human monocyte.
Zhiqiang ZOU ; Tao LI ; Zhenqi CHEN ; Wen ZHUANG ; Tingjun LI ; Jinzhong ZHANG ; Xianghui YANG
Journal of Medical Research 2006;0(10):-
Objective To explore the expression of protease activated receptors (PARs) on human monocytes. Methods Flow cytometry and RT - PCR was used to detect the expressions of PARs on human monocytes that purified by MACS. Results FACS analysis showed that monocytes expressed PAR - 1 , PAR - 3 , PAR - 4, but not PAR - 2.RT - PCR revealed that monocytes expressed PAR - 1 and PAR -3, but not PAR -2 and PAR -4 mRNA. Conclusion PARs is expressed on human monocyte cells,which may facilitate further investigation of the potential functions of PARs on monocytes.
5.Effect of blood vessels at different locations and angle between blood vessel and sonic axis on HIFU peripheral ablation
Dazhao MA ; Jianzhong ZOU ; Suying YANG ; Jing WEN ; Tao ZENG ; Qi WANG
Chinese Journal of Interventional Imaging and Therapy 2017;14(7):435-438
Objective To investigate the effect of blood vessels at different locations and angles between blood vessel and sonic axis within the target area on HIFU peripheral ablation.Methods Totally 56 phantoms containing rabbit thoracic a orta were divided into group A (different vessel locations) and B (different vessel angles).According to the distance (d)between the blood vessel center and the deepest (top) layer of the peripheral scanning,group A was divided into A1 (d=0),A2 (d=10 mm),A3 (d=20 mm) subgroups.According to the angle (α) between the vessel long axis and the acoustic axis,group B was divided into B1 (α=0-5°),B2(α=60° 65°),B3 (α=90°-95°),B4 (α=115°-120°) subgroups.A4,B5 subgroups were set as the control groups.The vessel diameter and vessel wall thickness were measured.The lesion morphology was observed and the energy efficiency factor (EEF) was calculated.Results There was no significant difference in vessel diameter and vessel wall thickness in both group A and B (all P>0.05).There was no residual area in the target area of A1,B1 subgroup,while there was residual area in the target area of the other subgroups.EEF of group A in ascending order were A4,A1,A2 subgroup,and statistically significant differences in pairwise comparisons (all P<0.05) except for A1 and A3 subgroup (P>0.05).EEF of group B in ascending order were B5,B2,B3 subgroup,respectively,and statistically significant differences in pairwise comparisons (all P<0.05) except for B2 and B4,B5 and B1 subgroup (both P>0.05).Conclusion It is 10 mm that the distance between the blood vessel center and the deepest (top) layer of the pe ripheral scanning,and 90°-95° that the angle between the vessel long axis and the acoustic axis has the greatest influence on the ablation of the target area.
6.Expression and purification of Litopenaeus vannamei allergen protein Lit v1.2
Huifang CHEN ; He LAI ; Yuyi HUANG ; Zehong ZOU ; Ying HE ; Ailin TAO ; Wen LI
Chinese Journal of Immunology 2015;31(12):1659-1662
Objective:To obtain purified recombinant Litopenaeus vannamei allergen protein Lit v 1.2.Methods: The target gene of Lit v 1.2 was inserted into clone vector pGEM-T and then ligated to the expression vector pET 44a.The pET44a-Liv 1.2 was transformed into Rosetta and screened by ampicillin resistance .The recombinant protein was expressed by IPTG induction .The protein was purified by 6-His tag affinity chromatography and the purification was analyzed by SDS-PAGE gel electrophoresis .Results:The ex-pression plasmid pET44a-Lit v 1.2 was constructed.SDS-PAGE showed that expressed Lit v 1.2 was efficient and soluble in E.coli Rosetta.The protein molecular weight was consistent with the theoretical value .The highly purified target protein was obtained.Conclusion:In this study ,we successfully gained highly purified recombinant allergen protein Lit v 1.2 which was expressed in prokaryotic system and purified by affinity chromatography column .The purified Lit v 1.2 protein will facilitate us to further study its role in immunological responses .
7.Malignant ectomesenchymoma of left vulva in childhood: report of a case.
Wen-Ping YANG ; Liang FENG ; Yan WU ; Yin ZOU ; Song-Tao ZENG ; Hua-Sheng ZHONG ; Hong-Yan XU ; Qiang XIAO
Chinese Journal of Pathology 2009;38(10):713-714
Desmin
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metabolism
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Diagnosis, Differential
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Female
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Follow-Up Studies
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Humans
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Infant
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Ki-67 Antigen
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metabolism
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Mesenchymoma
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drug therapy
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metabolism
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pathology
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surgery
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Nerve Sheath Neoplasms
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metabolism
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pathology
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Phosphopyruvate Hydratase
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metabolism
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Rhabdomyosarcoma, Embryonal
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drug therapy
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metabolism
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pathology
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surgery
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Teratoma
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metabolism
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pathology
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Vulvar Neoplasms
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drug therapy
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metabolism
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pathology
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surgery
8.Clinical and pathologic characteristics of pediatric rhabdoid tumor of kidney.
Yan WU ; Wen-ping YANG ; Qiang XIAO ; Yong CHEN ; Song-tao ZENG ; Hong-yan XU ; Hui HUANG ; Yin ZOU ; Hua-sheng ZHONG
Chinese Journal of Pathology 2011;40(5):336-337
Antineoplastic Combined Chemotherapy Protocols
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therapeutic use
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Child, Preschool
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Dactinomycin
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administration & dosage
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Diagnosis, Differential
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Female
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Follow-Up Studies
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Humans
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Infant
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Kidney Neoplasms
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drug therapy
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metabolism
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pathology
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surgery
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Male
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Proto-Oncogene Proteins c-bcl-2
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metabolism
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Rhabdoid Tumor
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drug therapy
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metabolism
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pathology
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surgery
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Rhabdomyosarcoma
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pathology
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Sarcoma, Clear Cell
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metabolism
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pathology
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Synaptophysin
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metabolism
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Vimentin
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metabolism
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Vincristine
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administration & dosage
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Wilms Tumor
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pathology
9.Experimental study on metastasis and correlation of metastasis with angiogenesis of hepatic tumor in rabbits after transcatheter arterial chemoembolization using As_2O_3 and lipiodol
Wen-Juan WU ; Hui-Xian CUI ; Ying-Hua ZOU ; Zhi-Wei ZHONG ; Xiang-Dong LIU ; Hai-Tao LI ; Xin-Yun FAN ; Yue-Min NAN ;
Chinese Journal of Radiology 2001;0(02):-
Objective To investigate the effect of transcatheter arterial chemoembolization(TACE) using As_2O_3 and Lipiodol on the growth and metastasis of the implanted hepatic tumor in rabbits and the correlation of metastasis with angiogenesis of the residual tumor.Methods Forty-eight New Zealand white rabbits were randomly divided into four groups and VX_2 carcinoma was implanted in the left lobes of the livers.Two weeks later,a catheter was inserted into the hepatic artery and infusion was performed via the hepatic artery using physiological saline(group A),Lipiodol(group B),ADM-Lipiodol(group C),and As_2O_3-Lipiodol(group D),respectively.One week after the treatment,the value of microvessel density (MVD)of tumors(samples got by biopsy)was examined by immunohistochemistry.Three weeks after the treatment,the volume and necrotic area of the implanted tumor were measured.The metastases in the liver, lungs and other organs were recorded.Results One week after the treatment,the value of MVD of the tumorswas(21.8?5.3),(23.4?3.9),(22.4?4.5),and(14.3?3.4)/400 power LM(F= 11.246,P=0.000).Three weeks after the treatment,the mean volume of the implanted tumor was (35.5?7.1),(21.2?8.3),(20.7?9.1),and(11.8?3.7)cm~3(F=21.203,P=0.0000)in groups A,B,C and D,respectively.There was significant difference between group D and group B(q= 4.398,P
10.Accuracy of diagnostic indicators for perioperative venous thromboembolism in orthopedic patients
Chengguang WANG ; Pengcheng ZHOU ; Wen ZOU ; Qing WU ; Tao CHEN ; Guiyong QIN
Chinese Journal of Trauma 2021;37(8):715-719
Objective:To analyze the accuracy of diagnostic indicators for perioperative venous thromboembolism(VTE)in orthopedic patients.Methods:A retrospective case-control study was conducted to analyze the clinical data of 109 patients who underwent initial total hip arthroplasty(THA),total knee arthroplasty(TKA)or proximal femoral nail antirotation(PFNA)in Hechuan District People ’s Hospital of Chongqing from January 2015 to September 2019. There were 55 males and 54 females,with age range of 18-76 years[(46.3 ± 11.2)years]. Of all,54 patients underwent unilateral THA due to femoral head necrosis and femoral neck fracture,36 patients underwent unilateral TKA due to osteoarthritis and 19 patients underwent PFNA due to proximal femoral fracture. VTE was confirmed in 47 patients(VTE group),and did not occur in 62 patients(non-VTE group). Venous blood was collected from all patients on admission and at postoperative 1,3,5,7 days to measure levels of plasma D-dimer,serum interleukin(IL)-18 and vascular endothelial growth factor(VEGF). The accuracy of the above indicators in VTE diagnosis was analyzed by using receiver operating characteristic(ROC)curve. Results:In both groups,preoperative levels of D-dimer,IL-18 and VEGF were significantly lower than those postoperatively,and each indicator showed significant difference at each time points after operation( P < 0.05 or 0.01). Moreover,levels of D-dimer,IL-18 and VEGF in VTE group were significantly higher than those in non-VTE group at each time after operation( P < 0.05 or 0.01). Area under the ROC curve(AUC)for D-dimer was 0.839(95% CI 0.729-0.887),with the optimal cut-off of 5.5 μg/ml,sensitivity of 87.0%,specificity of 79.0% and Youden index of 0.67. AUC for IL-18 was 0.817(95% CI 0.719-0.857),with the optimal cut-off of 293.5 pg/ml,sensitivity of 67.0%,specificity of 87.1% and Youden index of 0.457. AUC for VEGF was 0.837(95% CI 0.784-0.918),with the optimal cut-off of 510.8 pg/ml,sensitivity of 81.0%,specificity of 79.0% and Youden index of 0.583. AUC for combined D-dimer,IL-18 and VEGF was 0.870(95% CI 0.747-0.992),with the sensitivity of 87.2%,specificity of 83.4% and Youden index of 0.606. Conclusions:For diagnosis of perioperative VTE in orthopedic patients,serum VEGF is relatively accurate,while serum IL-18 has a low accuracy. However,the diagnostic rate of VTE can be improved by combining indicators of D-dimer,IL-18 and VEGF.