3.Rapid Genomic DNA Fragments Extraction From Serum Denatured With High Temperature
Yu WANG ; Shu WEN ; Xiaoyan LONG
Journal of Chinese Physician 2001;0(09):-
Objective To develop an approach for a rapid extraction of genomic DNA fragments from serum.Methods Serum specimen from human was processed using high temperature denaturation and was centrifuged, then using various primer sets to amplify the DNA fragment extracted, so as to verify the efficiency of this DNA extraction method.Results The templates preparing from this DNA extraction method were able to guarantee the amplification of fragments shorter than 400 base pairs.Conclusion This method is simple, rapid and inexpensive.These genomic DNA fragment extracted can be utilized to amplify definite DNA template for polymerase chain reaction.
4.Digital interpolation beamformer in medical ultrasound imaging.
Shu-Li SHU ; Wen-Wen TANG ; Liang WANG
Chinese Journal of Medical Instrumentation 2007;31(5):328-329
A digital beamformer in medical ultrasound imaging systems conventionally requires that the returning signals received by transducers are sampled at a rate significantly higher than the Nyquist rate. This paper presents a digital beamformer that employs zero padding followed by digital interpolation filtering to achieve vernier beamformer delays. The experimental results show that the digital interpolation beamformer can greatly decrease the complexity of A/D converter circuitry.
Image Interpretation, Computer-Assisted
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instrumentation
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Ultrasonography
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instrumentation
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methods
5.Prediction of walking and life span of children with cerebral palsy.
Zhen WANG ; Xiang-shu WEN ; Yong-ku LI
Chinese Journal of Pediatrics 2003;41(3):196-198
Cerebral Palsy
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mortality
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pathology
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physiopathology
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Child
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Humans
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Prognosis
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Survival Analysis
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Survival Rate
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Time Factors
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Walking
6.Cyclical theta oscillation induced by acute weak electrical stimulation of the rat vagus nerve central end.
Xian-wen DOXNG ; Hai-mei WANG ; Dan SHU
Chinese Journal of Applied Physiology 2007;23(4):423-490
Animals
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Electric Stimulation
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Hippocampus
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physiology
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Male
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Nerve Endings
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physiology
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Rats
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Rats, Sprague-Dawley
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Vagus Nerve
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physiology
8.Study of the process parameters in pharmaceutical air jet pulverization
Wen SHU ; Yuanhua WANG ; Shizhong JIANG ; Xiangyong CAI ;
Chinese Traditional Patent Medicine 1992;0(12):-
Objective:To study the influence of some process parameters on the product granularity in pharmaceutical air jet pulverization process.Methods:The method of quadratic regressive orthogonal experiment was used.Results:The mathematics model describing the relationship between the parameters studied and product granularity was established.Conclusion:The conclusions in the experimental study have instructional significance for pharmaceutical production.
10.Injury of hepatic mitochondria and its mechanism in rats with endotoxemia.
Ming-Hua BI ; Shu-Wen ZHANG ; Ban-En WANG
Chinese Journal of Applied Physiology 2004;20(1):90-97
Animals
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Endotoxemia
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metabolism
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pathology
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Female
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Liver
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metabolism
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pathology
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Male
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Mitochondria, Liver
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metabolism
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Rats
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Reactive Oxygen Species
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metabolism