1.Research advances of photosensitizer in photodynamic therapy of glioblastoma
Hong-cheng ZHAO ; Yue-qing WANG ; Qing-yun LI ; Hao DENG ; Xiao TAN ; Xiao-wen LIU
Acta Pharmaceutica Sinica 2022;57(6):1750-1757
Glioblastoma is a malignant tumor in central nervous system, which has strong invasion, poor prognosis and short survival time. At present, the main treatment strategy of glioblastoma is surgical excision, supplemented by radiotherapy and chemotherapy. However, due to incomplete resection and high recurrence rate, it is urgent to find novel therapeutic method for glioblastoma. Photodynamic therapy, as a promising non-surgical treatment, provides a new strategy for postoperative adjuvant therapy of glioblastoma. This review summarizes the mechanism and clinical application of photodynamic therapy mediated by various photosensitizers in glioblastoma, in order to provide help for the treatment of glioblastoma.
2.Comparison of efficacy of scleral buckling surgery for retinal detachment under microscope and under indirect ophthalmoscope
Xuan-Zhi, CHEN ; Wen-Jie, HUANG ; Xiao-Qing, MIAO
International Eye Science 2015;(4):666-668
AIM: To observe the effects of scleral buckling surgery for retinal detachment under microscope and under indirect ophthalmoscope.
METHODS: Forty - six patients ( 46 eyes ) with rhegmatogenous retinal detachment were retrospectively analyzed. Twenty-six patients received surgeries under microscope was retrospectively analyzed and compared to that of 20 patients who received surgeries under indirect ophthalmoscope by the same operator.
RESULTS: No statistical difference was found in patients' operation method choice, including condensation reaction reattachment situation and the retina reset operation at a time ( P> 0. 05 ). The best corrected visual acuity of microscopic group after 1mo of operation was better than before operation, and the improving rate of vision after operation was higher than that of the traditional group (P<0. 05).
CONCLUSION:The curative effect of external approach microsurgery has the similar efficacy with the conventional external approach surgery, and gets better visual acuity.
3.Effect of propofol on noise-induced hearing loss in guinea pigs
Ying XIAO ; Jian WEN ; Qing ZHANG ; Guixia JING
Chinese Journal of Anesthesiology 2014;34(2):154-157
Objective To evaluate the effect of propofol on noise-induced hearing loss in guinea pigs.Methods Forty-eight healthy adult male guinea pigs,aged 3 months,weighing 400-500 g,were randomly divided into 4 groups (n =12 each) using a random number table:control grotup (C group),propofol group (P group),noise-induced hearing loss group (N group),and propofol + noise-induced hearing loss group (P + N group).A loading dose of propofol 5 mg/kg was infused intravenously over 5 min,followed by infusion at 20 mg· kg-1 · h-1 for 115 min in P and P + N groups,while the equal volume of normal saline was given in N group.N and P+ N groups were exposed to the noise of 4 kHz center frequency and 118-122 dB sound pressure level for 120 min.Distortion product otoacoustic emission (DPOAE) was measured before noise exposure (T1) and at 1 h after the end of noise exposure (T2) and the amplitudes were recorded at the frequencies of 2,4,6 and 8 kHz.After the second measurement of DPOAE,all the animals were sacrificed and organs of Corti were harvested for determination of cochlear 8-isoprostaglandin F(2alpha) (8-iso-PGF2α) content (by ELISA assay) and out hair cell (OHC) count.The rate of OHC lesions was calculated.Results The DPOAE amplitude was significantly lower at frequencies of 4,6 and 8 kHz at T2 than at T1 in N and P + N groups (P < 0.05).Compared with C group,the DPOAE amplitude was significantly decreased at frequencies of 4,6 and 8 kHz at T2,while the cochlear 8-isoPGF2α content and rate of OHC lesions were increased in N and P + N groups (P < 0.05).Compared with N group,the DPOAE amplitude was significantly increased at frequencies of 4,6 and 8 kHz at T2,while the cochlear 8-iso-PGF2α content and rate of OHC lesion were decreased in P + N group (P < 0.05).Conclusion Propofol can reduce noise-induced hearing loss in guinea pigs possibility through decreasing oxidative stress response-induced damage to cochlear OHCs.
5.Application of the Innovative and Integrative Experiments on Special Skills Training in Food Sciences
Shu-Qing LIU ; Xiao-Lu JIANG ; Hai-Jin MU ; Jin-Xiao FENG ; Wen-Xiao LIU ;
Microbiology 1992;0(03):-
In order to make students have integrative qualities and reform the shortage of former teaching methods in the food microbiology experiment,a new experiment teaching system was established,introducina the integrative and innovative experiment to the students gradually.The reformation of experiment in food microbiology has trained the students well and improved their special skills.Most important of all,it can offer them a chance to realize their own ideas.The students can design an innovative and integrative experiment to carry out their originalities.Good effects have been made since this system is very helpful to improve their consciousness of innovation and integrative abilities in doing experiments.
6.The countermeasure exploration for the current state of occupational health and occupational medicine experimental teaching
Qing WANG ; Wen CHEN ; Mengjun HOU ; Lei XU ; Qing WEI ; Dawei WU ; Yongmei XIAO
Chinese Journal of Medical Education Research 2011;10(10):1252-1254
Current occupational health and occupational medicine teaching content focused on the detection of harmful factors,and more belonged to validation experiments.Closed-end management of experimental teaching,experimental report-based performance evaluation methods and single model of teaching methods reduced the students' study interesting,lack of problem-solving abilities.Therefore,it is necessary to optimize the experimental curriculum,adopt an open experimental teaching,build a reasonable experimental evaluation system and promote the diversification of teaching model.
7.Some Approaches for the Selective Isolation of Rare Actinomycetes
Wei XIAO ; Ming-Gang LI ; Xiao-Long CUI ; Yi-Qing LI ; Meng-Liang WEN ;
Microbiology 1992;0(01):-
The focus of microbiologists has moved to the rare actinomycetes.For selective isolation of rare actinomycetes that all play the important role in bioactive compounds,the approaches which involve the methods using gellan gum and flooding solution、 rehydration-centrifugation(RC)、 extremely high frequency radiation(EHF)、 bacteriophage and sucrose-gradient centrifugation were introduced in this paper.
8.A case of Shwachman syndrome.
Bao-xi ZHANG ; Xiao-qing ZHAO ; Xiao-li WU ; Wen-jie GUO
Chinese Journal of Pediatrics 2004;42(7):550-550
Abnormalities, Multiple
;
genetics
;
pathology
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Child, Preschool
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Chromosome Aberrations
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Diagnosis, Differential
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Diarrhea
;
diagnosis
;
etiology
;
therapy
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Exocrine Pancreatic Insufficiency
;
complications
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Fever
;
diagnosis
;
etiology
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Fingers
;
abnormalities
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Humans
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Male
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Pancreatic Diseases
;
etiology
;
Syndrome
9.Role of bone marrow mesenchymal stem cells in the recovery of rat acute renal tubular damage
Xiang LIU ; Xue-Qing YU ; Jing NIE ; Zhi-Jian LI ; Xin AN ; Xiao-Yan LI ; Wen-Xing PENG ; Xiu-Qing DONG ;
Chinese Journal of Nephrology 2005;0(12):-
Objective To investigate whether mesenchymal stem cells can promote the recovery of acute renal tubular damage induced by mercuric chloride and to explore its possible mechanism.Methods Acute renal failure rat model was established by intraperitoneal injection of mercuric chloride.SD rats were randomly divided into three groups which were MSCs injection group, saline infusion group and normal control group.Seven days later,the changes of rat weight,survival,renal function and pathology were observed;PCNA,ED-1 and GFP were detected by immunohistochemistry; The expression of cytokines in kidney and the distribution of GFP plasmid-transfected MSCs in kidney were examined by RT-PCR.Results MSCs infusion ameliorated the decline of rat weight,survival, renal function,and pathological changes.PCNA and ED-1 positive cells in MSCs group were fewer than those in saline group.Expression of growth factors EGF,PDGF,HGF were obviously up- regulated and pre-inflammatory cytokines TNF-?was significantly reduced in MSCs-treated kidneys. GFP-labelled MSCs occurred occasionally in renal interstitium of MSCs-treated rats,but not in renal tubules.Conclusions Bone marrow mesenchymal stem cells can promote the recovery of acute renal tubular epithelial cells damage caused by mercuric chloride.The mechanism may partly depend on regulating the excretion of cytokines in renal microenvironment rather than completely depend on their differentiation to tubular cells.
10.Gloning and Sequence Analysis of Dienelactone Hydrolase Gene
Wen-Hui ZHONG ; Ming SUN ; Guo-Qing HE ; Xiao-Shan FENG ; Zi-Niu YU ;
Microbiology 1992;0(03):-
A 2,4 -dichlorophenol degrading Pseudomonas strain GI241-1 was isolated from a soil sample. The dienelactone hydrolase gene, designated as dcpD which encodes dienelactone hydrolase involved in transforming cis-2-chloro-dienelactone into 2-chloromaleylacetic acid, was cloned from this bacterium strain. The gene cloning strategy was to construct genomic library after location of its neighbouring gene by Southem blot and to screen the aim transformant by dot blotting. Sequencing results showed that length of dcpD is 702bp. The sequence of dcpD and the deduced amino acid are different from the relative sequences registered in the GenBank.