Objective To prepare 60 mer oligo microarray chips for detecting human immunodeficiency virus (HIV) and for the clinical application in the detection of AIDS patient. Methods Oligonucleotide probes were designed according to the sequence information of two types of HIV. Oligo microarray was prepared by using Cartesian Microarrayer. Products of the restrictive display PCR were labeled with Cy3. Furthermore, the PCR products were sequenced. Results Using the oligo microarray, HIV infection could be detected in laboratory as well as in clinical assays. Results of hybridization indicated that 1 AIDS patient was positive and 20 health people were negative. The results obtained by sequencing confirmed the results obtained by oligo microarray studies. Conclusion The HIV 60 mer oligo microarray could be used in detecting patient HIV infection and analyzing its genotypes.

Objective: To investigate the effects of Vaccinium bracteatum Thunb. Leaf ethanol extract (VEE) and its different extracts on the anti-oxidative capability of brain stem in sleep-deprived rats. Methods: Rats were intragastrically administered with low, middle, and high dose of VEE (1.2 g/ml, 2.4 g/ml, and 7.2 g/ml) and different extracts daily for 10 days. The rat mental fatigue models were established by sleep deprivation through flower-pot on the 7th day. Rats treated with salidroside (200 mg/ml) were taken as positive controls. After 72 h sleep deprivation, the concentrations of malondialdehyde (MDA) , superoxide dismutase (SOD) , and total antioxidative capacity(T-AOC) in the brain stem were determined in each group. Results: The concentrations of the MDA, SOD, and T-AOC in the normal control group were (16.4±0.42) nmol/mg protein, (2.9±0.62) U/mg protein, and (154.3±14.47) U/ml, respectively. Compared with the normal control group, the sleep-deprived group had significantly higher concentrations of MDA, SOD and T-AOC(P<0.05), and the groups of salidroside, high dose ethanol, and aqua extract of VEE had significantly lower MDA (P< 0.05), and lower SOD and T-AOC. Conclusion: The high dose VEE can greatly decrease the oxidative stress level of the brain stem, and it also has remarkable anti-oxidative ability, with the active part mainly in the aqua extract of VEE.

With polyethylene glycol vitamin E succinate (TPGS) as the carrier materials, and berberine hydrochloride ( BER) as model drug, we formed berberine hydrochloride (BER) -loaded TPGS nanomicells (BER-PMs) using filming-rehydration method to improve its solubility and in vitro anti-tumor effect. The transmission electron microscope (TEM) was used to observe the particle appearance; particle detector was used to detect the diameter and Zeta potential; and ultracentrifugation was utilized to determine the encapsulation efficiency (EE) and drug-loading (DD); dynamic dialysis method was used to study the in vitro release behavior of BER-PMs, and the anti-tumor activity against MCF-7 cells was determined by MTT method. Results showed that the average particle size of BER-PMs was (12.45 ± 1.46) nm; particle size was uniform and spherical; drug loading and encapsulation efficiency were (5.7 ± 0.22)% and (95.67 ± 5.35)%, respectively. Zeta potential was (-1.12 ± 0.23) mV; release rate within 24 h was 37.20% and 41.14% respectively in pH 7.4 and pH 6.5 phosphate buffer in vitro; compared with BER, BER-PMs can significantly inhibit MCF-7 cell proliferation (P < 0.05), promote cell apoptosis and improve the anti-tumor activity of BER in vitro. Therefore, the formed berberine hydrochloride micelle can more effectively promote the apoptosis of MCF-7 cell, and improve the drug's in vitro anti-tumor effect.
Antineoplastic Agents ; chemistry ; pharmacology ; Berberine ; chemistry ; pharmacology ; Cell Death ; drug effects ; Cell Survival ; drug effects ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Humans ; MCF-7 Cells ; Particle Size ; Polymers ; chemistry ; pharmacology ; Solubility

AIM: To study the role of scavenger receptor A(SR A) in the uptake of oxidized low density lipoprotein(OxLDL) in mouse peritoneal macrophages(MPM). METHODS: Comparing the difference of the uptake of OxLDL in SR A-deficient and wild-type MPM. RESULTS: The results showed that the binding of OxLDL wasn't apparently reduced in SR A-deficient MPM. The association of OxLDL was reduced by 35.8% and degradation of OxLDL was reduced by 42% in SR A-deficient MPM compared with those in wild-type MPM. CONCLUSION: Studies showed that SR A didn't play an important role in the uptake of OxLDL in MPM. Approximately 70% of the uptake of OxLDL in macrophages is attributable to non-SR A receptor.

Based on signal to noise ratio and probabilistic neural network method associated with experimental data,all analysis model in gastric carcinoma is presented.According to the available information,the samples of gastric carcinoma can be tested and ana.Lyzed.The signal to noise ratio is first calculated．Secondly,records in the database are chosen as a training set to build a probabilistie neural network model and the feature subset is selected according to accuracy.Finally,test set is to test accuracy of model．The model is implemented using MATLAB,and it can be generalized and applied to similar disease auxiliary diagnosis region.

Objective To evaluate laparoscopic local resection for the treatment of gastric tumors. Methods The clinical data of 78 patients who received laparoscopic local resection at the PLA General Hospital from February 2006 to January 2010 were retrospectively analyzed. According to the tumor site and free range, total laparoscopic gastrectomy was applied to 45 patients, laparoscopic local resection was applied to 22 patients, laparoscopic and endoscopic tumor resection was applied to 11 patients. The efficacies of the surgical approaches were investigated. Results Laparoscopic local resection was successfully performed on the 78 patients without conversion to open surgery. The mean operation time and operative blood loss were 75 minutes (range, 45-120 minutes) and 60 ml (range, 35-90 ml), respectively. The mean diameter of the tumor was (2.5±1.3)cm (range, 0.7-4.8 cm). No mortality or morbidity occurred postoperatively. The bowel function recovery time and the duration of hospital stay were 35 hours (range, 25-42 hours) and 7.5 days (range, 6-9 days), respectively. The results of postoperative pathological examination verified that 63 patients were with gastric stromal tumor, 11 patients were with benign gastric diseases, including 5 with gastric heterotopic pancreas, 2 with inflammatory pseudotumor, 2 with hyperplastic polyp, 1 with schwannomas and one with angioma. Four patients were with carcinoid, 3 carcinoids were located in mucous layer and 1 invaded into muscular layer. The median time of follow-up was 26 months, and no anastomotic stenosis or port-site metastasis was observed. Of the 63 patients with gastric stromal tumor, 2 were treated with imatinib mesylate, 1 had tumor recurrence and received reoperation. Conclusion Laparoscopic local resection is safe and feasible for the treatment of benign gastric neoplasms, stromal tumor and early gastric tumors.

Objective To investigate the expression of proliferating cell nuclear antigen(PCNA) in renal tissues of children with primary nephrotic syndrome(NS),and elucidate the relationship between PCNA expression and cell proliferation in renal tissues from the children with primary NS.Methods Paraffin-embedded renal biopsy tissue sections from 39 patients with primary NS were examined by immunohistochemical staining with anti-PCNA monoclonal antibody,normal renal tissue sections from 6 nephrectomized patients with nephroma were selected as control. Possible correlation between the percentage of PCNA positive cells and the pathologic type , histopathological score, clinical indices (serum albumin ,serum cholesterol ,serum creatinine and 24 hours urine protein ) before renal biopsy of NS were evaluated separately .Results The percentage of PCNA positive cells in glomeruli and tubulom terstitium of NS patients was significantly higher than that of the control (P

OBJECTIVETo better understand the molecular pathogenesis of cervical cancer, and provide novel means for clinical diagnosis and treatment of this malignancy.

METHODSThe gene chip data of cervical cancer were obtained from GEO database and statistically analyzed using BRB-ArrayTools to identify the genes related to cervical cancer with bioinformatics analysis using Panther software.

RESULTSThirty-seven differentially expressed genes were identified in cervical, cancer samples, including 23 up-regulated and 14 down-regulated genes. These genes were associated with the cell skeletons transporters and such processes as cell signal transduction, transcriptional control, cell adhesion, and cell apoptosis.

CONCLUSIONBioinformatics analysis can help with effective analysis of the gene chip data. The pathogenesis of cervical cancer involves abnormal expression of multiple genes, and these data may benefit further investigations of the early diagnosis and treatment of the malignancy.

Biomarkers, Tumor ; analysis ; genetics ; Computational Biology ; methods ; Female ; Gene Expression Profiling ; methods ; Gene Expression Regulation, Neoplastic ; Genes, Tumor Suppressor ; Humans ; Oligonucleotide Array Sequence Analysis ; Uterine Cervical Neoplasms ; genetics

Objective To study the inhibitory effect of small interference RNA(siRNA) ofcyclin E gene on the growth ofK562cells. Methods siRNA targeting the 940 bp site of the cyclin E mRNA were designed and generated by PCR amplification.The PCR products containing U6 promoter and the siRNA were then transfected into K562 cells via LipofectamineTM2000.The cells transfected with non-functional siRNA served as the negative control group and those only treated with serum-free RPMI1640 as the blank control group. Cell counting, reverse transcriptase (RT)-PCR and flow cytometry were employed to evaluate the effect of RNA interference. Results Compared with the negative and blank control groups, the viable cell count in the interference group was decreased by approximately 80%, the ratio of G1-phase cells increased by nearly 30%, and growth arrest was observed. Cyclin E mRNA expression in the cells of the interference group was significantly lowered by about 70%as compared with that of the negative and blank control groups, whereas the latter two groups had similar expression levels.Conclusion RNA interference induces obvious inhibition of cyclin E gene expression, which consequently affects the proliferation ofK562 cells.

Objective To analyze and predict the structure and function of mosquito densovirus(MDV)nostmetual proteinl(NSI).Methods Using different bioinformafics software,the EXPASY pmtparam tool,ClustalX1.83,Bioedit,MEGA3.1,ScanProsite,and Motifscan,respectively to comparatively analyze and predict the physic-chemical parameters,homology,evolutionary relation,secondary structure and main functional motifs of NS1.Results MDV NS1 protein wag a unstable hydrophilic protein and the amino acid sequence wag highly conserved which had a relatively closer evolutionary distance with infectious hypodermal and hematopoietic necrosis virus(IHHNV).MDV NS1 hag a specific domain of superfamily 3 helicage of small DNA viruses.This domain contains the NTP-binding region with a metal ion-dependent ATPase activity.A virus replication roller rolling-circle replication(RCR)initiation domain wag found near the N terminal of this protein.This protien has the biological function of single stranded incision enzyme.Conclusion The bioinformatics prediction results suggest that MDV NS1 protein phys a key role in viral replication,packaging,and the other stages of viral life.