Aim To study the effect of sodium quercetin-7-sulfate (SQMS) on activity of recombinant human phosphoinositide 3-kinase (PI3-K) p110? catalytic subunit.Methods Recombinant human PI3-K p110? catalytic subunit was expressed by gene engineering.PI3-K activity was assayed by incubating recombinant PI3-K p110? with phosphatidylinositol-4,5-bisphosphate and [?-~ ~32 P]ATP; the ~ ~32 P-radiolabeled lipids were extracted with chloroform and methanol, and assessed by scintillation counter.Results SQMS showed inhibition on the recombinant p110? catalytic subunit with IC_~50 13.14 ?mol?L~-1 .Conclusions SQMS is an inhibitor of PI3-K. The recombinant PI3-K p110? catalytic subunit might be used as a molecular target for simpler target screening more effective inhibitors of PI3-K.

Objective To study the effect of disodium quercetin-7,4′-disulfate (SQDS) on activity ofrecombinant human phosphoinositide 3-kinase (PI3-K) p110?catalytic subunit. Methods Recombinanthuman PI3-K p110?catalytic subunit was expressed by gene engineering. PI3-K activity was assayed byincubating recombinant PI3-K p110?with phosphatidylinositol-4,5-bisphosphate and [?-32P] ATP;the32P-radiolabeled lipids were extracted with chloroform and methanol,then assessed by scintillation counter.Results SQDS showed inhibition on the recombinant p110?catalytic subunit with IC5014.88?mol/L.Conclusion SQDS is an inhibitor of PI3-K. The recombinant PI3-K p110?catalytic subunit might be usedas a molecular target for simpler screening and development of more effective inhibitors of PI3-K.

AIM To study the effects and kinetics of sodium quercetin 7 sulphate (SQMS) on recombinant human protein kinase CK2 holoenzyme. METHODS The recombinant human CK2 holoenzyme activity was assayed by detecting incorporation of 32 P of [? 32 P]ATP into the substrate at various conditions. RESULTS SQMS was shown to strongly inhibit the holoenzyme activity of recombinant human protein kinase CK2 with an IC 50 of 1 37 ?mol?L -1 , Kinetic studies of SQMS on recombinant human CK2 showed: the inhibition was competitive with ATP and noncompetitive with casein. CONCLUSIONSQMS is an inhibitor of protein kinase CK2, and the inhibitory action of SQMS on CK2 is competitive with ATP and noncompetitive with casein.

Combining theory and practice at home and abroad in recent years ,construction plan of the military infectious diseases syndromic surveillance system was discussed from aspects of data collection and transmission , data analysis and early warning , incident response based on the core business requirements of syndromic surveillance for infectious diseases . The study provides the demand traction for the system construction .

Objective To compare the effect of traditional open surgery and laparoscopic repair of gastric perforation on postoperative pulmonary function.Methods Fifty patients were divided into two groups according to therapeutic method.Group A underwent traditional open gastric perforation repair(n=25).Group B underwent laparoscopic repair of gastric perforation(n=25).The pulmonary functions were examined at 1 day,3 days and 7 days postoperatively.Results All patients had different degrees of postoperative pulmonary function decline.After operation,the forced vital capacity(FVC)and forced expiratory volume in 1 second(FEV1)and vital capacity(VC)in group A were significantly lower than those in preoperation(P<0.05).The patients in laparoscopic operation group decreased significantly only on the first day postoperative.The postoperative pulmonary function of patients undergoing open surgery was significantly lower than that of patients undergoing laparoscopic surgery(P<0.05).Conclusion Laparoscopic repair of gastric perforation has less damage to the lung function than traditional open gastric perforation repair.Laparoscopic surgery plays an important role in the recovery of postoperative pulmonary function.

Animals ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; In Vitro Techniques ; Mice ; Myocarditis ; drug therapy ; virology ; Phytotherapy ; methods ; Virus Diseases ; drug therapy

Animals ; Genes, BRCA2 ; Genes, erbB-1 ; Genes, erbB-2 ; Genes, p16 ; Genes, p53 ; Genes, ras ; Genetic Predisposition to Disease ; genetics ; Humans ; Pancreatic Neoplasms ; classification ; genetics ; pathology

Adult ; Biomarkers ; blood ; urine ; Female ; Humans ; Male ; Manganese ; blood ; urine ; Middle Aged ; Occupational Exposure ; adverse effects ; Young Adult

Objective To investigate the effects of sustained inflation (SI) combined with small tidal volume ventilation on lung recruitment and hemodynamics in patients with acute lung injury (ALI). Methods Patients with severe trauma followed by ALI were selected for this study. Patients underwent small tidal volume ventilation (baseline) for 1 h and then SI with 20 cm H 2O?30 s(SI 1), 30 cm H 2O?30 s(SI 2), 40 cm H 2O?30 s(SI 3) and 50 cm H 2O?30 s(SI 4) for 1 h, respectively. Parameters of pulmonary mechanics and hemodynamics were measured at 1 h after baseline, SI and returning baseline, respectively. Results Compared with those of the baseline, no changes of parameters were found after SI 1 and SI 2, but EELV and Cst increased significantly after SI 3 and SI 4. Paw, PAP and PVRI decreased significantly after SI 3 and SI 4. After SI interruption, all the physiological variables returned to baseline. SI had no significant effect on HR, AP and CI. Conclusion Treatment with SI combined with small tidal volume ventilation in patients with ALI can provide lung recruitment and improve lung compliance, but it has no significant side effect on hemodynamics.

Objective To investigate the protective effect of endotoxin pretreatment on hippocampal neurons in rat forebrain following ischemia reperfusion and its possible mechanism. Methods Rat forebrain ischemia reperfusion model was used. The effects of endotoxin pretreatment on the changes of superoxide dismutase (SOD), glutathione peroxidase (GSH PX) and malondialdehyde (MDA) activities and the neuron count in CA1 region were observed. Results Pretreament with endotoxin before cerebral ischemia enhanced the activities of SOD and GSH PX but decreased MDA level and the number of ischemic neurons in CA1 region. Conclusion Endotoxin pretreatment can protect the neurons in rat forebrain against ischemia reperfusion injury. The mechanism may be associated with the enhancement of endogenous antioxidant activity in central nervous system.