Cataract is still the main blinding eye disease,and it is a major goal of fighting blindness in China.In the past years,the diagnosis,treatment technology and equipment have made great progress.With the further understanding of this disease and increasing requirement of patients for the quality of life,the standardization,safety and efficacy of the clinical diagnosis and surgical outcomes of cataract have become much more important.So the relative clinical examination approaches,modified operating skills,new surgery development and application as well as novel type of functional IOLs design advance rapidly,which provide an expensive space for the selection of the treatment for cataract.High quality of cataract surgery depends on preoperative precise biometry,reasonable operating scheme targeting at personality,suitable functional IOL,less intra-or post-operative complications,effectively avoiding treatment risk and correctly processing disadvantageous factors affecting postoperative visual quality.Therefore,the management of cataract surgery is a systemic procedure.The issue of improving ophthalmic examination,new cataract surgical technique and new IOL emerges as the time requires.How to make up individual-based treatment options for patients,better improve postoperative visual quality,enhance the safety of operative procedure,reduce operative complications is the challenges and unremitting goal for the ophthalmologists.

Antimicrobial susceptibility testing (AST) is an important means to clinical microbiology. At present, there are no standard AST methods for aquatic pathogens comparing with human and veterinary pathogens. We reviewed the recent research progress on establishing these methods and discussed the key factors in AST of aquatic pathogens. Furthermore the defect of AST of aquatic pathogens in China was profiled and prospect for further research was put forward.

Objective To explore the therapeutic effect of Yupingfeng powder in secondary immunodeficiency caused by simple nephrotic syndrome(SNS) in children.Methods Seventy-eight cases of SNS patients were divided into 2 groups randomly.One was control group which were treated with immunosuppressant only.The other group was Yupingfeng powder group which were treated with both immunosuppressant and Yupingfeng powder.The morbidity of infection,the relapse rate of SNS,and serum immunoglobulin IgA and IgG and the T-lymphocytes subtype were detected before and after treatment in two groups.Results The morbidity with infection was 55.3% in Yupingfeng powder group,which shows a remarkable decrease compared with control group(85%).The relapse rate of SNS was 39.5% in Yupingfeng powder group which was much lower than that in control group(67.5%).The level of serum IgA and IgG before treatment were(3.88?1.22) g/L and(0.83?0.14) g/L and rose up to(10.06?1.89) g/L and(1.38?0.10) g/L after treatment in Yupingfeng powder group.Whereas the control group with(6.23?1.55) g/L and(0.85?0.13) g/L was remarkably lower than those in Yupingfeng powder group.CD4+,CD8+ and the ratio of CD4+/CD8+ were(37.91?3.89)%,(30.69?3.08)% and(1.24?0.49)% respectively before treatment.They rose up to(42.38?4.89)%,(26.01?2.20)% and(1.63?0.41)% respectively in Yupingfeng powder group,while those in control group were(39.87?3.91)%,(29.76?3.55)% and(1.34?0.24)% respectively after treatment.Conclusion It is feasible to treat the Yupingfeng powder to cure the of secondary immunodeficiency caused by SNS in children.

Objective To find out a more rational pathological classification criteria for renal injury in patients with type 2 diabetes mellitus. Methods The renal clinicopathological features of forty-nine type 2 diabetic patients with maeroalbuminuria were collected and were compared retrospectively. The patients without diabetic renal disease were excluded. According to the pathological features, the patients were divided into two groups: typical diabetic glomerulopathy (DG) and atypical diabetes-related renal disease (ADRD). Results The renal biopsy revealed DG accounted for 59.2% of the patients, while the remaining 40.8% presented atypical renal injury defined as ADRD. In DG group, volume fraction of mesangium per glomerulus, glomerular basement membrane width, atrophic tubules index, intersititium injury index and prevalence of hyalinization of renal arteriole were higher; podocyte density per glomerulus was lower; duration of type 2 diabetes was longer; the level of fast blood glucose, systolic blood pressure, mean arterial pressure, proteinuria and prevalence of diabetic retinopathy (DR) were higher; glomerular filtration rate (GFR) was lower. In ADRD group, body mass index and prevalence of obesity were higher; dyslipidemia was more severe. GFR was negatively correlated with glomerular global sclerosis rate in both DG and ADRD group. Proteinuria was positively correlated with volume fraction of mesangium per glomerulus in DG. No correlation between proteinuria and pathological features was found in ADRD. DR (94.8%) and duration of type 2 diabetes over five years (90.7%) had high negative predictive value for DG. Conclusions Renal injuries in type 2 diabetes patients are heterogeneous. ADRD is an atypical renal injury in type 2 diabetes patients whieh is different from DG. DR and duration of diabetes are more helpful in predicting DG separating from ADRD.

Objective To study the pathologic pattern and clinical feature of type 2 diabetes mellitus complicated with chronic kidney diseases (CKD). Methods Clinicopathological features of 155 type 2 diabetic patients complicated with CKD were collected and analyzed retrospectively. The patients were divided into four groups: typical diabetic glomerulopathy (DG),atypical diabetes-related renal disease (ADRD), non-diabetic renal diseases (NDRD) and DG complicated with NDRD. Results Renal biopsies revealed DG accounted for 18.7% of the patients, ADRD accounted for 12.9%, NDRD accounted for 60.0%, and DG complicated with NDRD accounted for 8.4%. In DG group, duration of type 2 diabetes was longer;the level of fast blood glucose, systolic blood pressure, mean arterial pressure and prevalence of diabetic retinopathy (DR) were higher;proteinuria was heavier and evaluated glomerular filtration rate (eGFR) was lower. In ADRD group, body mass index and prevalence of obesity were higher;dyslipidemia was more severe. Gross hematuria and acute renal insufficiency could be only found in NDRD group.Without DR, duration of diabetes under 5 years, gross hematuria, acute renal insufficiency,evidences of autoimmune diseases and proteinuria≥3.5 g/24 h but eGFR ≥60 ml/min were specific valuable predictors for NDRD. Conclusions Renal injuries in type 2 diabetic patients are structural heterogeneous, in which NDRD is more common and is different from ADRD and DG.Renal biopsy should be considered when type 2 diabetic patients complicated with CKD present at least one characteristic as follows: duration of diabetes under 5 years, without DR, history of gross hematuria, acute decrease of renal function, evidences of autoimmune diseases and proteinuria ≥ 3.5 g/24 h but eGFR ≥ 60 ml/min.

Private cloud is an internal cloud featuring multi-tenant,dynamic configuration and optimization infrastructure,which enables developers to achieve service self-deployment and self-hosting within security coverage of the enterprise data center.This paper introduced the concept of cloud computing.Then it went on to present the private cloud architecture of the hospital by analysis of problems in the hospital including information construction costs,management and maintenance,and information expansion.In the end,the authors analyzed the cloud computing service model,hospital private cloud architecture,along with outcome analysis for hospital private cloud implementations.

Aged ; Biomarkers ; metabolism ; Carcinoid Tumor ; metabolism ; pathology ; Carcinoma, Endometrioid ; metabolism ; pathology ; surgery ; Diagnosis, Differential ; Female ; Granular Cell Tumor ; metabolism ; pathology ; Humans ; Hysterectomy ; methods ; Keratin-7 ; metabolism ; Keratins ; metabolism ; Krukenberg Tumor ; metabolism ; pathology ; Middle Aged ; Mucin-1 ; metabolism ; Ovarian Neoplasms ; metabolism ; pathology ; surgery ; Sertoli Cell Tumor ; metabolism ; pathology ; Sex Cord-Gonadal Stromal Tumors ; metabolism ; pathology ; surgery

AIM: To investigate the amiloride-sensitive Na~+ channel current (Iamil) in primary cultured human bronchial epithelium cells of chronic obstructive pulmonary disease (COPD) patients and the influence of interleukin 4 and interleukin 6. METHODS: Human bronchial epithelium cells were isolated and cultured from 18 patients undergone pueumonectomy in hospital. Interleukin 4 or interleukin 6 at concentration of 10 ?g/L was used to treat these cultured cells, and Iamil was measured by whole cell patch clamp techniques. RESULTS: There was no markedly difference among normal no-smoking group, normal smoking group and COPD group. Interleukin 4 down-regulated the Iamil in normal no-smoking group, normal smoking group and COPD group. The down-regulated percentages were 59.7%, 54.7% and 30.0%. Interleukin 4 down-regulated the Iamil in normal no-smoking group (44.8%) and normal smoking group (34.9%) but not in COPD group, and the current forms were not changed after IL-4 or IL-6 treatment. CONCLUSIONS: Interleukin 4 and interleukin 6 down-regulated the Iamil in primary cultured human bronchial epithelium cells. It may contribute to the hypersecretion of COPD, and the up-regulated interleukin 4 and interleukin 6 in COPD patients may cause them react weaker to the treatment of interleukin 4 and interleukin 6.

Gastroesophageal reflux(G), duodenoesophageal reflux(D) and duodeno gastroesophageal reflux(DG) model and control group(C) were reproduced by operations in rats. Their esophagi were harvested at 1, 2, and 4 weeks after operation. Epithelial cells were stained by PI and assessed with flow cytometry(FCM).The results of FCM were compared in terms of DNA index (DI, the ratio of the G0/G1 peak of the sample cells to that of the spleen lymphocyte of normal rat), proliferating index (PI, the percent of cells in S and G 2 /M in all cells) and aneuploidy (a distinct additional G0/G1peak).PI of D group increased at 2 weeks, and PIs of D and DG group were greater than G group and C group at 4 weeks.DIs and the rates of aneuploidy of all groups were similar at all time points. The results were in accord with the morphological changes. It is concluded that gastric and duodenal contents can induce abnormal proliferation of esophageal epithelium. This effect is obvious in groups with reflux of duodenal contents. Excessive proliferation of the esophageal epithelium might be one of the pathogenetic factors of reflux esophagitis or other relevant complications.