BACKGROUND:Critical limb ischemia is difficult to be treated among the peripheral arterial occlusive diseases, and the application of transplanting autologous stem cells can bring a hope for the treatment of this disease.
OBJECTIVE: To review the autologous stem cells transplantation in the treatment of critical limb ischemia at present, and to study the mechanism and the existing problems.
METHODS:A computer retrieval of PubMed database, Wanfang database, and CNKI database was conducted to search the related articles with the keywords of“stem cell, transplant, critical limb ischemia”in English and Chinese. Final y, 47 articles were included in final analysis according to the inclusion criteria.
RESULTS AND CONCLUSION:Autologous stem cells transplantation for treatment of lower extremity ischemic disease is a latest and significantly effective treatment, but the mechanism is unclear and stil needs further study.

Objective To observe the clinical effect of nasal douche washing and nasal cleaning under the endoscope treatment for sinusitis after nasopharyngeal carcinoma radiotherapy.Methods Thirtyeighty cases of sinusitis after nasopharyngeal carcinoma radiotherapy was treated by nasal douche washing and nasal cleaning under the endoscope.The course of treatment were for 6 months.The clinical effect were evaluated with the chronic sinusitis visual analogue scale(VAS)and Lund-Mackey nasal sinuses CT image mark system before and after treatment.Results After treatment for 6 months,the VAS scores and LundMackey nasal sinuses CT image mark system scores significantly decreased compared with before treatment [(8.21 ± 1.57)scores vs.(3.01 ± 1.16)scores,(7.37 ± 1.44)scores vs.(3.23 ± 1.07)scores](P ＜ 0.05).Conclusions The nasal douche washing and nasal cleaning under the endoscope treatment for sinusitis after nasopharyngeal carcinoma radiotherapy has positive clinical effect,which is a better treatment method.

Objective To explore the accuracy of hysteroscopy as a diagnosis for endometrial carcinoma and to determine if the procedure increase the rate of positive peritoneal cytology in the patients. Methods The peritoneal cytology of 36 patients with endometrial carcinoma diagnosed by D&C or hysteroscopy was retrospectively analyzed. Results The peritoneal cytology was positive in 1 (5.9%) of the 17 cases diagnosed by D&C and 2 (10.5%) of the 19 cases diagnosed by hysteroscopy (P=1.000). Based on the pathological examination,the accuracy of D&C was 52.9% (9/17),which was significantly lower than that of hysteroscopy [89.5% (17/19),P=0.025]. In the cases with stage Ⅰ endometrial carcinoma,the diagnostic accordance rate of hysteroscopy was 85.7% (12/14),while that of the D&C was 42.9% (6/14,P=0.040). The 36 patients were followed up for 2 months to 4 and a half year (over 1 year in 28 cases). One patient with Ⅲc stage carcinoma died of recurrent tumor in 2 years after the treatment. Conclusion Hysteroscopy shows higher diagnosis accuracy with no evidence indicating an increased rate of positive peritoneal cytology compared to D&C.

Animals ; Hypothyroidism ; physiopathology ; Learning ; Memory ; Mice ; Motor Activity

Objective To observe the clinical efficacy of external -rou te microsurgery for retinal detachment (RD). Methods In 36 patie nts (36 eyes) with single rhegmatogenous RD, the silica gel piece and/or bucklin g bands were preplaced, and drainage of subretinal fluid, retinal cryotherapy, e xamination of locating the holes, and intraocular injection of gas were performe d under surgical microscope. The surgical effects were compared with those of ot her simultaneous 37 patients with rhegmatogenous RD who underwent surgery under binocular indirect ophthalmscope. Results The simultaneous int raoperative observation of the fundus details and the sclera through the microsc ope was excellent in all cases. Under the surgical microscope, the reaction of r etinal cryotherapy was clearly visible without any serious surgical sequela. The observation of reaction of retinal cryotherapy and the orientation of the holes were not affected by mild opacity of the refractive media. Retinal reattachment was achieved in 31 eyes after the primary surgery and in 3 eyes after the secon dary surgery, with the final rate of reattachment of 94%. The best-corrected vi sual acuity was

OBJECTIVE:To select the optimum watering extraction process of yangyinqingwei oral liquid. METHODS: Influence of extraction time upon the extraction of volatile oil was observed; The orthogonal design was adopted to observe the extraction process of weight of the extracts and the content of paeoniflorin. RESULTS & CONCLUSIONS:The optimum watering extraction process of yangyinqingwei oral liquid was that medicinal materials were dipped for 30 minutes before decoction,then the extraction of volatile oil in saposhnikovia divaricata and pogostemon cablinlasted for 2 hours, next the residue of drugs and the other drugs were decocted together, and finally water of 8 times as much as the drugs were added and the mixture was extracted 3 times, with 1.5 hours for each time.

The bacterial strain CSS-4-2 isolated from the spleen of Carassius auratus of penze(♀) ? Cyprinus acutidorsalis (♂) with septicemia was proved to b e a pathogen by artificial infection. This bacteria has short rod-shaped cells , Gram negative and motile, with single polar flagellum, and with not capsule. No spore was formed , and it was resistant to 0/129. It could utilize glucose, mal t ose ,mannite, sucrose and inositol, but could not utilize malonate, lactose, xyl ose, raffinose, sorbin and adonitol. Oxidase and arginine dihydrolase was posit ive.While urease, lysine decarboxylase and ornthine decarboxylase was negative .Bacteriological identification by VITEK-AMS-60 showed the strain CSS-4-2 w as Aeromonas caviae. Drug sensitivity assay showed that this strain was sen sitive to s treptomycin, tobramycin, chloromycetin, rifampicin, norfloxacin and nitrofuranto in, but resistant to penicillin G.

The growth property of pathogenic bacterium Aeromonas caviae strain CSS-4-2 which was isolated from the spleen of Carassius auratus of penze(♀) ? Cyprinus acutidorsalis (♂) that diseased septicemia was studied.The growth curve of CSS-4-2:lag phase was in 0～3h,log phase was in 3～30h, stationary phase was in 33～36h,decline phase was after 36h when CSS-4-2 was cultured shaking in tryptone liquid medium. It's optimum growth temperature ranges from 25℃ to 30℃, optimum growth pH ranges from 5 to 10, and optimum salinity ranges from 0% to 1% Nacl concentration .

Objective To investigate the role of sitting-up-straight position in correction of peripherally inserted central catheter(PICC)heterotopia.Method Eighteen cases with PICC heterotopia were instructed to take the position of sitting up straight. Result All of the patients had the PICC heterotopia corrected.Conclusions The sitting-up-straight position can effectively correct the PICC catheter heterotopia.This method is simple and feasible and thus worthy of clinical application.

Objective : To analyze the trend of lung cancer incidence in Shaoxing, Zhejiang Province from 2014 to 2019, so as to provide the basis for lung cancer prevention and control. Methods : The data of lung cancer were collected through the information management system of chronic disease surveillance in Zhejiang Province.The crude incidence rate, age-standardized incidence rates by Chinese standard population ( ASIRC ) and by world standard population ( ASIRW ), truncated rate ( 35-64 ) years old ), and cumulative rate (0-74 years old) were calculated. The annual percentage change ( APC ) was used to analyze the trend of lung cancer. The annual contribution rate was used to analyze the contribution of the incidence trend with age to the total trend. Results : Totally 22 261 cases of lung cancer were reported in Shaoxing from 2014 to 2019. The crude incidence rate, ASIRC, ASIRW, truncated rate ( 35-64 years old ), and cumulative rate ( 0-74 years old ) were 83.51/100 000, 41.28/100 000, 41.01/100 000, 57.00/100 000 and 5.11%, respectively. The crude incidence rate, ASIRC and ASIRW increased significantly from 2014 to 2019, with the APC values of 8.56%, 6.42% and 6.05%, respectively ( P<0.05 ). The crude incidence rate, ASIRC, ASIRW, truncated rate ( 35-64 years old ), and cumulative rate ( 0-74 years old ) of man were 105.25/100 000, 48.09/100 000, 50.69/100 000, 62.03/100 000 and 6.42%, respectively, which were higher than those of women ( 61.84/100 000, 33.05/100 000, 31.35/100 000, 51.71/100 000 and 3.76% ).The crude incidence rate increased with age. The annual contribution rates of lung cancer incidence in people aged 15-<45 years, 45-<55 years, 55-<65 years and ≥65 years were 51.20%, 30.86%, 15.79% and 2.15%, respectively. The crude incidence rate in people aged 15-<45 years, 45-<55 years and 55-<65 years increased significantly from 2014 to 2019, with the APC values of 23.84%, 13.79% and 6.78%, respectively ( P<0.05 ). Conclusion From 2014 to 2019, the incidence of lung cancer in Shaoxing shows an increasing trend, and the age of onset is getting younger.