Objective To investigate the clinical application and effect of micro-planting nail anchorage in orthodontics.Methods Fifty-six patients with oral orthodontic were randomly divided into two groups.29 patients in the observation group were used micro-planting nail anchorage,27 patients in the control group were used the strong non-implant anchorage.Results The reduction of upper incisor inclination and distance in observation group was significantly higher than control group.In another hand,displacement of molars in observation group was significantly lower than control group,the difference was significant(t =9.714,4.491,17.172,all P ＜0.05).Conclusion Micro planting nail can provide the ideal anchorage and orthodontic treatment,and it has the advantages of easy and flexible operation,and it can be instantly afterburner,reliable quality,worthy of clinical application and promotion.

Objective To evaluate the changes of platelet aggregation rate and short-term prognosis after the application of colopidgrel with booster doses or replaced by ticagrelor for ACS patients with CYP2C19 medium-metabolized genotype). Method A total of 302 patients with medium-metabolized genotype were randomly divided into colopidgrel group (75 mg/bid) and ticagrelor group (90 mg/bid). Patients in both groups accepted other conventional treatments of coronary heart disease and accompanied diseases. The platelet aggregation rates and platelet inhibition rates were observed before and after the treatment. The incidence of adverse events was followed up within 1 m. Results After one-week treatment, the platelet aggregation rates of the inducement with 5 μmol/L ADP had statistical significance between colopidgrel group and ticagrelor group ( P = 0 . 019 ) and the platelet inhibition rates had statistical significance difference between the two groups (P = 0.000). No severe adverse events occurred within one-month follow-up and 10 dyspnea patients were all in ticagrelor group. Conclusions Compared with booster doses of clopidogrel, ticagrelor presents obvious inhibitory effect on platelet of patients with medium-metabolized of ACS but it increases the incidence of dyspnea.

OBJECTIVE:To establish a HPLC method for the determination of stavudine and related substances METHODS:The Hypersil C18(4 6mm?150mm,5?m)was used as analysis column The mobile phase was methanol-water(13∶87,V/V) Detection wavelength was 264nm The flow rate was 1ml/min The column temperature was 20℃ RESULTS:The calibration curves was linear in the concentration range of 0 025～0 3mg/ml(r=0 9 999,n=6) The average recovery was 99 26%(RSD=0 76%) The contents of stavudine in 3 batches of semifinished materials and stavudine capsules(imported and domestic)were 99 48%,99 55%,99 32%(materials);97 21%,101 54%,98 92%(imported stavudine capsule);100 57%,97 86%,102 33%(domestic stavudine capsule)respectively CONCLUSION:The method is simple,accurate,sensitive and repeatable and it is suitable for the determination of stavudine and its preparations

Objective To investigate the role of endoscopic nasobiliary drainage ( ENBD) of bile duct instead of T tube drainage during laparoscopic common bile duct exploration (LCBDE). Methods All the patients accepted ENBD before LCBDE / laparoscopic cholecystectomy (LC) + LCBDE. During the operation the stones were extracted by choledochoscopy or crashed with electrohydraulic lithotripsy. After removing all of the biliary stones, the nasobiliary tube still remained in the common bile duct, then primary closure of the exploration wound on the duct wall was made, and with routine abdominal drainage. After operation , radiography of the nasobiliary tube was taken to investigate if there were any residual stones or leakage in the bile duct remained. Subsequently the drainage tube and nasobiliary tube were respectively removed as indicated. Results Totally 43 cases accepted ENBD preoperatively, after 6. 1 days LCBDE was performed, in 36 cases (83. 7% ) the operation was successfully using nasobiliary drainage and primary closure of the duct wound. Postoperative radiography of the duct system through the nasobiliary tube showed only in one case (2. 6% ) a small stone left which was then extracted under ERCP. There were no bile duct stricture and bile leakage, so the nasobiliary tube was removed smoothly. Seven cases ( 18. 6% ) were transferred to open abdominal exploration, 2 of them still kept the nasobiliary tube and the choledochal incisions were primary closed. Totally 38 patients (88.4% ) were successfully drained by ENBD. The abdominal drainage and nasobiliary tubes were removed in average of 3.2 and 6.7 days respectively without complications. Conclusion ENBD is an effective and safe procedure for bile duct drainage in LCBDE. It has the advantage of minimal trauma, short drainage period and rare complications. It suggested that this procedure should be a new satisfied way in treatment of bile duct stones.

B ased on Chinese medicine pharmacological literature data , the property , flavor , toxicity , meridian tropism, efficacy, and clinical application of Chinese herbal medicine were used as a set of attributes. The sup-port vector machine ( SVM ) was used in the establishment of predictive models of 187 pharmacological effects of Chinese herbal medicine respectively. And the cross-validation method was used to determine the accuracy of predictive models . After that , the predictive models with the predictive accuracy rate greater than 90% were used to predicate pharmacological effects of 624 herbals recorded in the Chinese Pharmacopoeia(2010 edition). It was found that the accuracy rate of 108 models was greater than 90%, and the accuracy rate of antibacterial effect predictive model was 99.76%. The highest predictive value of Chinese herbal medicine was the anti-oxi-dation effect of Menispermi Rhizoma.

Objective To investigate the relation between type 2 diabetic nephropathy and blood lipid levels with insulin resistance.Methods The study included 50 patients with type 2 diabetes mellitus without nephropathy,45 patients with early stage diabetic nephropathy,52 patients with diabetic nephropathy and overt proteinuria,43 patients with end-stage diabetic nephropathy.Serum level of their lipid,blood sugar,HbA1c,insulin,and albumin-to-creatinine ratio(ACR)were monitored.Results(1)The lever of serum TG、TC、LDL-C、 ApoB were increased in the three groups of diabetic nephropathy,there was significant difference between the group of diabetes mellitus without nephropathy and the three groups with diabetic nephropathy(P

Objective To obtain anoikis-resistant cells from human osteosarcoma cell line hFOB1.19 and investigate its biological characteristics.Methods The osteosarcoma cell line we used was malignant transformed immortalized human fetal osteoblastic human cells(hFOB1.19)which was transformed in our lab(called transformant in this paper).We have used anti-adhesion cell culture method to mimic detachment of tumor cells from extracellular matrix(ECM)to obtain anoikis-resistant variants.Transmission electron microscopy and fluorescence microscopy were used to observe the morphological changes,flow cytometry was used to observe the apoptotic rate.The growth characteristic was observed by MTT.The migration characteristics was examined by transwell cell culture system.Results Typical apoptotic cells were seen after de-adhesion culture.The apoptotic rate of the transformant at 24,48 and 72 h showed a gradually increasing tendency.The apoptotic rate of anoikis-resistant cells was lower than that of the transformant at the same time point.Compared with the transformant,the anoikis-resistant variants had stronger ability in proliferation and in migration with statistical significance(P

Objective:To investigate the effect of body mass index(BMI), waist circumference(WC) and waist hip ratio (WHR) on blood pressure, serum lipids, renal function, protein and lymphocyte. Methods:175 middle and elderly patients were measured. Results:Compared to normal weight cases, SBP and uric-acid were significantly higher in over-weight cases. Triglyceride, SBP and DBP were all significantly higher in obesity cases. When waist circumference was beyond 85 cm in women, triglyceride, lymphocyte and DBP were all significantly higher than those in normal weight women. When waist circumference was beyond 90 cm in men, triglyceride was significantly higher than that in normal weight men. Conclusion:When BMI increases, triglyceride and blood pressure will increase accordingly. When WC is higher than normal, triglyceride in male and female will be higher. Control of obesity, is helpful to prevent cardiovascular diseases.

Traumatic coagulopathy is a serious complication of tissue damage caused by trauma.In clinic,it easily develops into multiple system organ failure and results in high mortality.Traumatic coagulopathy is a plurality of blood coagulation disorder,its pathogenesis is extremely complex and involves tissue damage,blood coagulation,anticoagulation and fibrinolysis,inflammation and many other pathophysiological aspects.An in-depth study on the pathogenesis of traumatic coagulopathy is very important to improve clinical effect.This paper tries to do a review of recent study progress from pathophysiological mechanisms and hopes to provide reference for clinical work.

Objective To understand the prophylactic use of antimicrobial agents in the patients undergoing I incision surgery from 2011 to 2013 in a tumor hospital,to explore the the effects of intervention management measure for antibacterial agents using in the perioperative period.Methods The cases of tumor surgical patients of 2011-2013 were selected and divided into the three intervention groups and non-intervention group.The data of medication after three-stage continuous integrated intervention were collected and compared.Results The percentage of antibiotics use was much lower,from 61.73%pre-intervention to 47.03%,26.78%and 24.85%after three stage intervention. The course of preventive medication treatment was significantly decreased from 81h to 53h,33h,21h,respeetively.The reasons of their rationally utilizing antibiotics were significantly improved after intervention management.Conclusion Rational antibiotics utilization can be improved by intervention management during perioperative period of typeⅠinci-sion operations,which can achieve the goal of health department.