Gastrointestinal stromal tumor(GIST) is the most common mesenchymal malignancy of the GI tract. The mainstay of treatment is surgery. For patients in whom complete resection is not possible, or in patients with metastatic or recurrent disease, they are unresponsive to standard chemotherapy and to radiotherapy. There has been no effective systemic treatment for unresectable GIST or metastatic disease. Gain-of-function mutations of the KIT proto-oncogene occur in up to 90% of GIST, allowing constitutive activation of tyrosine kinase (i.e. auto-phosphorylation of tyrosine residues independent of ligand-receptor binding), leading to aberrant cell division and tumour growth. Imatinib selectively inhibits the tyrosine kinase activity associated with KIT, which forms the rationale for evaluating its effects in GIST. Herein, we review recent developments in treatment for GIST and implication for optimal treatment in these patients.

Objective To investigate the clinical-pathological feature and treatment of mvxoid adrenocortieal adenoma. Methods The clinical features of a Myxoid adrenocortical adenoma were re-viewed with its clinical manifestation,imaging,pathology and therapy.The patient was a 43-vear-old woman.The patient complained of recurrent headache,fatigue with hypertension and hypokalemia for 10 years.B-ultrasound examination revealed a 4.1 crux 3.4 cm hypoechoic solid mass in the left adre-nal gland. Relative literatures were reviewed. Results The patient was operated successfully by 1ap-aroscope.The section of tumor showed gray-like flour with yellow jelly-like substance.Hemorrhage was seen in some area. Under light microscope,the tumor cells were similar in size with boundary.There was no obvious heteromorphism and mitotic feature.There was full of mucoid substance.Im-munohitochemical staining showed that the tumor cells were positive for Vimentin,Melan-A and AB/ PAS staining,which confirmed the diagnosis of myxoid adrenocortical adenoraa. Blood pressure andbiochemical indicators returned to normal after surgery.There was no sign of recurrence after 6 month follow-up. Conclusions Myxoid adrenocortical adenoma is a rare adrenal tumor.Path010gy is the key to diagnosis.Surgical removal of tumor could be the preferred therapeutic choice.

Microarray technology, used in microorganisms detection with its advantages of rapid detection, high sensitivity, high-throughput and low cost, has been applied in environmental microbial community research widely in past few years. It focuses on investigation of structure, diversity, function, dynamics of microbial populations within complex environmental samples. Furthermore, it also reveals their responses and adaptation to environmental perturbations such as climate change, toxic contaminants. According probe design patterns, several types of microarrays, such as phylogenetic oligonucleotide arrays (POAs), functional gene arrays (FGAs), metagenomic array(MGA) and community genome arrays (CGAs) have been constructed for environmental studies. This review discusses applications of microarrays to environmental microbial populations research along with its potential for screening of specific microorganisms, gene or expression functional gene representing different environmental microbial populations.

OBJECTIVE To investigate the carrier state of lid margins after eyes area disinfection by 75% alcohol and 0.5% iodine and to compare the effect of different disinfectants,so we will know whether 0.5% iodine can be used for eyes area disinfection. METHODS Thirty eight patients(76 eyes) from our hospital in March-May 2005 were analyzed.The operated eyes area as control group were disinfected by 75% alcohol-the traditional disinfection method.The contralateral eyes area without operation as experimental group were disinfected by 0.5% iodine.Lid margins samples were obtained for culture after 3 minutes. RESULTS The qualification rate was 81.58% in the experimental group whereas 60.53% in the control group(P

Congresses as Topic ; Otorhinolaryngologic Surgical Procedures ; Rhinitis ; surgery ; Skull Base ; surgery

Child ; Child, Preschool ; Endoscopy, Digestive System ; Female ; Follow-Up Studies ; Humans ; Infant ; Male ; Peptic Ulcer

In recent years, the biopharmaceutical industry has grown rapidly, and the market size of monoclonal antibody drugs has increased significantly. Accurate structural characterization and quality control are the supporting technologies for the development of monoclonal antibody drugs. As a significant post-translational modification of antibody drugs, glycosylation has an important influence on its efficacy, stability, and immunogenicity. The existing literature usually uses liquid chromatography-mass spectrometry to perform major glycosylation modifications of monoclonal antibody drugs. Characterization, there are few studies on low-abundance glycosylation, but the characterization and control of low-abundance glycosylation cannot be ignored. In this study, we have established a qualitative and quantitative analysis technology for N-glycans based on RapiFluor-MS reagent-labeled monoclonal antibody drugs. This method has a short sample processing time and high sensitivity. It can not only characterize the main glycoforms of three monoclonal antibody drugs (adalimumab, bevacizumab, and trastuzumab) but also can quantify low-abundance N-glycans. The results of the study showed that the main glycoforms specified in the Pharmacopoeia could be detected in different batches of monoclonal antibody drugs, but the content of N-glycans in different batches of samples is not identical. After that, we analyzed the N-glycans connection sites and glycoforms at the intact glycopeptide level, further enriching the N-glycans structure information of the monoclonal antibody. The qualitative and quantitative analysis technology of N-glycans based on RapiFluor-MS reagent-labeled monoclonal antibody drugs can realize the in-depth characterization and control of glycosylation modification of monoclonal antibody drugs.

The research and development of monoclonal antibodies (mAbs) is a rapidly developing field. From the first generation of murine mAbs to the fourth generation of fully human mAbs, the efficacy and safety of mAbs in the treatment of various diseases have been continuously improved. In order to regulate the development and evaluation of mAbs, drug regulatory agencies and pharmacopeias of America and China have tried to issue feasible test procedures and acceptance criteria for quality evaluation of mAbs and biosimilars. Mass spectrometry (MS) technique with high sensitivity, resolution, selectivity, and specificity has become an important tool to evaluate the quality characteristics of monoclonal antibody-related products or specify mAb quality. The research of MS-based monoclonal antibody study involves structure characterization, impurity analysis, pharmacokinetics/pharmacodynamics (PK/PD), etc. This review focuses on the current quality control requirements of mAb related products and the development of MS technique for mAb quality characterization and specification. It is expected to provide information and references for evaluating the quality of monoclonal antibodies under research and development.

Objective To highlight the diagnose and treatment of stationary paraganglioma in retroperitoneum.Methods Ten cases of stationary medullary chromaffinoma of retroperitoneum proven pathologically from 1997 to 2007 were reviewed and sutdied.Hypertension were not observed in all patients,who were admitted to hospital through routine phisical examination.Endocrine secretion examinations,B-US,CT,MRI,131IMIBG,and octreotide were used to dianose the diease.Among all 10 patients,4 cases took ?-receptor blocker for 2～4 weeks preoperatively.3 were operated laparoscopically,7 were operated with lumber incision.Results Hypertension and urinary catecholamine was usually normal or elevated lightly in stationary medullary chromaffinoma of retroperitoneum.131and octreotide have high sensitivity and accuracy in diagosing medullary chromaffinoma.4 were near renal hilum,6 was in para-aorta.They were from 3 cm to 15 cm in size.The results of pathology were paraganglioma in 7 and malignant paraganglioma in 3.Tumor markers were positive,such as cgA,syn,NSE and s-100.There was 1 case metastasis 1 years later.Conclusion Once the correct diagnosis is made,surgical treatment should be carry out on the basis of correct drug preparation.Intimate follow-up is necessary and important.

Shenqi mixture tonic (C. A. mixture )is a compounded Chinese herb preparation composed of Codnopsis pilosula (Franch. )Nannf., Astragalus membranaceus Bge. and Polygonum multiflorum Thunb. Its antiaging effect was studied on 24 months old SD rats. The body weight, food intake,cardiac capacity and activities ofbrain monoamine oxidase-B(MAO-B)and red blood cell superoxide dismutase (SOD)were estimated to assess its antiaging effect. Results indicated that after feeding C. A. mixture for a fixed time the activity of MAO-B was decreased and that of red blood cell SOD increased obviously. Calorie production and cold resistant capabbility of the aged animals were improved significantly though there were no gain in body weight. In addition ,the cardiac capacity was enhanced. From the above results it can be concluded that C. A. mixture possesses evident antiaging effect