1.The changes of endothelial cell function and coagulation-fibrinolysis system in maintenance hemodialysis patients with diabetic nephropathy
Wen HUANG ; Jie ZHENG ; Yanling ZHAI
Chinese Journal of Practical Internal Medicine 2000;0(12):-
Objective To find out the changes of endothelial cell function and coagulation-fibrinolysis system in maintenance hemodialysis(MHD)patients with diabetic nephropathy(DN).Methods The plasma levels of TM,PAI-1,TAT and PAP in 28 DN-MHD,34 non-DN MHD and 40 controls were measured by ELISA.Results (1)The levels of TM,PAI-1,TAT and PAP in both DN group and non-DN group were significantly higher than those in control group,P
2.Finite element analysis on unconfined compression of cartilage tissues
Wen-jie ZHAI ; Zhong-yong ZHAI
Journal of Medical Biomechanics 2012;27(6):E630-E638
Objective To analyze the load-bearing mechanism and stress relaxation properties of the articular cartilage (AC) through finite element simulation and experimental validation. Methods By comprehensively considering the solid phase of the matrix, the liquid phase of the pore and the reinforced phase of the collagen fibrils in AC, as well as the dilatation dependent permeability of AC, a fibril reinforced poroelastic (FRPE) model was built including changes of void ratio with subsurface depth of the AC. Based on the proposed model, and by utilizing ABAQUS software and FORTRAN language, the finite element analysis (FEA) on unconfined ramp compression of AC was conducted. The equilibrium modulus of porcine cartilage tissues under unconfined compression was measured by a self-designed biomechanical property measuring system, and the results between the FEA and the unconfined ramp compression test of the AC were compared. Results The liquid pore pressurization could last about 80 seconds and contributed up to 90 % of the total stress at the middle point of the test specimen when it was compressed at a strain rate of 0.45%/s. Conclusions The FEA on the unconfined ramp compression of AC based on the FRPE model can quantitatively evaluate the load bearing capacity of the solid and liquid phase, respectively, changed with different strain and loading time. Simulation analysis combined with the unconfined ramp compression test results facilitates the evaluation on mechanics properties of the cartilage with more accuracy.
3.Violent manipulation to release adhesion inducing condylar fracture of femur and malunion: a report of 1 case.
Xing-sheng WANG ; Ke-jian LIAN ; Wen-liang ZHAI ; Yan-jie GUO
China Journal of Orthopaedics and Traumatology 2008;21(9):697-697
Femur
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diagnostic imaging
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injuries
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physiopathology
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Fractures, Malunited
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diagnostic imaging
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physiopathology
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surgery
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therapy
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Humans
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Male
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Manipulation, Orthopedic
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methods
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Postoperative Complications
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diagnostic imaging
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physiopathology
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therapy
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Tomography, X-Ray Computed
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Young Adult
4.Treatment of patients with sentinel bleeding after hepatobiliary and pancreatic surgery
Youkui GAO ; Jie LI ; Jingwei ZHAI ; Xiaofeng JIANG ; Songhang LIU ; Haopeng WEN ; Liangqi CAO
Chinese Journal of Hepatobiliary Surgery 2023;29(3):199-203
Objective:To compare the results of operative versus interventional treatments in patients presenting with sentinel hemorrhage after hepatobiliary and pancreatic surgery.Methods:The clinical data of patients presenting with sentinel hemorrhage after hepatobiliary and pancreatic surgery at the Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Guangzhou Medical University from August 2017 to July 2022 were retrospectively analyzed. Of 82 patients who were enrolled in this study, there were 50 males and 32 females, aged (59.0±7.7) years. The patients were divided into the interventional group ( n=42) and the surgical group ( n=40) based on the treatment they received for sentinel hemorrhage. The vascular injury rate, the first operation time for sentinel bleeding, the rate of successful hemostasis in a single operation, the number of deaths and other indicators were compared between groups. Results:In both the two groups of patients who underwent percutaneous transhepatic cholangial drainage, hepatectomy, endoscopic retrograde cholangiopancreatography, hilar cholangiocarcinoma resection and cholecystectomy were mainly performed hepatic artery injury, pancreaticoduodenectomy with gastroduodenal artery injury, and splenectomy with splenic artery injury. In the intervention group, 36 patients (85.7%) were successfully hemostasis after single treatment, and 32 patients (80.0%) in the operation group, and there was no significant difference between the two groups (χ 2=0.47, P=0.492). The first operation time for the intervention group was (40.5±8.5) min and the mortality rate was 2.4% (1/42), which were significantly better than that of the operation group (90.6±20.8) min and 15.0% (6/40) (all P<0.05). Conclusion:Interventional therapy can be used as the first-line diagnosis and treatment for sentinel bleeding after hepatobiliary and pancreatic surgery. It has the advantages of a lower mortality rate in treating these patients.
5.Study on plague pathogeny in areas along Qinghai-Tibet Railroad in Qinghai from 2001-2006
Zhi-zheng, QI ; Zu-yun, WANG ; Jian, HE ; Rong-jie, WEI ; Hai-hong, ZHAO ; Qing-wen, ZHANG ; Xiao-yan, YANG ; Cun-xiang, LI ; Rui-xia, DAI ; Yong-hai, YANG ; You-quan, XIN ; Hai-tao, ZHAI ; Xing-hai, MAO ; Hong-wen, QI ; Yong, XIAO
Chinese Journal of Endemiology 2008;27(2):204-206
Objective To study the features of Yersinia pestis(Y.pestis)in areas along Qinghai-Tibet Railroad in Qinghai Province.Methods To identify the biologic types and the molecular biological feathers of Y.pestis isolated from areas along Qinghai-Tibet Railroad in Qinghai from 2001-2006.Results All the tested Y.pestis was biologically of classical type and ecologically of Qinghai-Tibet plateau type.The Y.pestis had high virulence.The Y.pestis of 65×106 plasmids was distributed in the Tanggula area,the Y.pestis of 52×106plasmids,in Tianjun and Delingha areas.The Y.pestis srains carried 52 × 106 plasmids.except the two containing 65 X 106 plasmids in Wulan County.The genetic type of Y.pestis in Tanggula was type 5 and that in Zongwulong of Delingha,Saishike,Keke,Tongpu of Wulan was type 8 except 2 strains of Y.pestis isolated from woodchuck and the patients in Dananwan of Tongpu,Wulan County were type 15.Conclusion The Y.pestis in the area along Qinghai-Tibet Railroad in Qinghai belongs to Qinghai-Tibet plateau type with high virulence.
6. Effect of combined treatment with cyclophosphamidum and allicin on neuroblastoma-bearing mice
Xiang-Yang GAO ; Xian-Jie GENG ; Xian-Wei ZHANG ; Yuan WEI ; Guang-Jun HOU ; Wen-Long ZHAI
Asian Pacific Journal of Tropical Medicine 2015;8(2):137-141
Objective: To evaluate the efficacy of allicin combined with cyclophosphamide on neuroblastoma (NB)-bearing mice and explore the immunological mechanism in it. Methods: A total of 30 NB-bearing mice were equally randomized into model group, cyclophosphamide group and combined therapy group, 10 nudemice were set as normal saline (NS) group. Cyclophosphamide group and combined therapy group were weekly injected with 60 mg/kg cyclophosphamide for four weeks; besides, combined therapy group was given with allicin (10 mg/kg/d) by gastric perfusion for 4 weeks; model group and NS group were given with the same volume of NS. Serum VEGF content was detected by ELISA pre-treating (0 d) and on the 3rd d, 14th d and 28th d; on 29th d, all mice were sacrificed and the tumor, liver, spleen and thymic tissues were weighted. Tumors were made into paraffin section for detecting tumor cell apoptosis and proliferation by TUNEL and BrdU method, respectively. Survival curves were drawn by Kaplan-Meier method. Results: After treatment, both treatment groups relieved on viscera indexes, VEGF level, T cell subsets distribution and tumor growth and each index of combined therapy group was better than cyclophosphamide group (P<0.05 or 0.01); only combined therapy group could significantly increase the lifetime of NB-bearing mice (μ 2=5.667, P=0.017). Conclusions: Allicin can improve T cell subsets distribution and inhibit VEGF expression through its immunomodulatory activity, thereby improve the efficiency on NB in coordination with cyclophosphamide.
7.Effect of Metabolic Reprogramming on Toxin Eliminating Therapeutics in Targeting Lung Cancer Stem Cells
Hui-jie LI ; Xi-wen HU ; Yuan-fu QI ; Xiu-rong LI ; Zhai-dong LIU
Chinese Journal of Experimental Traditional Medical Formulae 2020;26(8):75-80
Objective::To observe the effect of realgar nanoparticles (a representative drug in toxin eliminating therapeutics) targeting hypoxia-inducible factors (HIF), which act as effector molecules on metabolic reprogramming of lung cancer stem cells, and to explore the effect mechanism of lung cancer stem cells and metabolic reprogramming in the process of lung cancer metastasis, so as to verify the effectiveness of toxin eliminating therapeutics in the prevention and treatment of lung cancer metastasis. Method::Lung cancer A549 cells were cultured
8.Wendan decoction (温胆汤) for treatment of schizophrenia: a systematic review of randomized controlled trials.
Yi-wen CHE ; Ke-yu YAO ; Yu-peng XI ; Zi-jie CHEN ; Yong-le LI ; Ning YU ; Shuang-qing ZHAI
Chinese journal of integrative medicine 2016;22(4):302-310
OBJECTIVETo assess the beneficial and adverse effects of Wendan Decoction (温胆汤, WDD) for the treatment of schizophrenia.
METHODSFive electronic databases were searched until May 2014, including the Chinese National Knowledge Infrastructure, the Chinese Biomedical Literature Database, the Chinese Scientist Journal Database, PubMed, and the Cochrane Central Register of Controlled Trials in the Cochrane Library. The randomized controlled trials (RCTs) testing WDD against placebo, antipsychotic drugs, or WDD combined with antipsychotic drugs against antipsychotic drugs alone were included. Study selection, data extraction, quality assessment, and data analyses were conducted according to the Cochrane standards.
RESULTSThirteen RCTs (involving 1,174 patients) were included and the methodological quality was evaluated as generally low. The pooled results showed that WDD combined with antipsychotic drugs were more effective in clinical comprehensive effect, Positive and Negative Syndrome Scale (PANSS) scores and Brief Psychiatric Rating Scale scores compared with antipsychotic drugs alone. However, WDD had less effectiveness compared with antipsychotics in clinical comprehensive effect; and WDD was not different from antipsychotic drugs for PANSS scores. The side effects were significantly reduced in the intervention group compared with the control group.
CONCLUSIONSWDD appears to be effective on improving symptoms in patients with schizophrenia. However, due to poor methodological quality in the majority of the included trials, the potential benefit from WDD needs to be confirmed in rigorous trials and the design and reporting of trials should follow the international standards.
Antipsychotic Agents ; therapeutic use ; Brief Psychiatric Rating Scale ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Publication Bias ; Randomized Controlled Trials as Topic ; Schizophrenia ; drug therapy
9.The diagnostic potential of Golgi protein 73 for cirrhosis in patients with chronic hepatitis C.
Peng Fei WANG ; Shu Hong LIU ; Xiang Jun QIAN ; Xiang Wei ZHAI ; Xia Jie WEN ; Ming Jie YAO ; Jing Min ZHAO ; Fengmin LU
Chinese Journal of Hepatology 2022;30(8):879-884
Objective: To explore the diagnostic value and model of serum Golgi protein 73 (GP73) in patients with hepatitis C cirrhosis. Methods: 271 cases with chronic hepatitis C virus infection who were treated in the Fifth Medical Center of PLA General Hospital from January 2010 to December 2017 were retrospectively collected as the research objects, including 126 cases with hepatitis and 145 cases with liver cirrhosis. Serum GP73 and liver stiffness measurement (LSM) based on transient elastography test were performed in all patients. Simultaneously, blood routine, liver function, coagulation function and other related indicators were collected. GP73 diagnostic efficiency for liver cirrhosis was evaluated by receiver operating characteristic curve (ROC). GP73 diagnostic value was clarified after comparison with aspartate aminotransferase/platelet ratio index (APRI), FIB-4 index (FIB-4) and LSM. Compensated hepatitis C virus-related cirrhosis diagnostic model based on serological index was established by logistic regression analysis. Results: The area under the receiver operating characteristic curve (AUC) of GP73, LSM, FIB-4 and APRI in the diagnosis of compensated hepatitis C virus-related cirrhosis were 0.923, 0.839, 0.836 and 0.800 respectively, and GP73 had the best diagnostic efficiency (P <0.001). LSM and GP73 combined use had improved the diagnostic sensitivity of cirrhosis to 97.24%. Multivariate logistic regression analysis revealed that GP73, age, and platelets were independent predictors of cirrhosis.Compensated hepatitis C virus-related cirrhosis diagnostic model (GAP) was established based on the result: LogitP=1/[1+exp(6.145+0.013×platelet-0.059×age-0.059×GP73)].AUC model for diagnosing compensated liver cirrhosis was 0.944, and the optimal cut-off value was 0.56, with sensitivity and specificity of 84.03% and 92.06%, respectively, and the diagnostic efficiency of this model was better than that of APRI, FIB-4, LSM and GP73 alone (P<0.05). Conclusion: GP73 is a reliable serum biomarker for the diagnosis of compensated hepatitis C virus-related cirrhosis. The GAP diagnostic model based on GP73, platelet count, and age can further improve the diagnostic efficiency and help to diagnose patients with compensated hepatitis C virus-related cirrhosis.
Aspartate Aminotransferases
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Biomarkers
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Fibrosis
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Hepatitis C
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Hepatitis C, Chronic/complications*
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Humans
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Infant, Newborn
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Liver/pathology*
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Liver Cirrhosis/pathology*
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Polyesters
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ROC Curve
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Retrospective Studies
10.Mannan-Binding Lectin Inhibits Candida Albicans-Induced DC Maturation and Cytokine Secretion.
Ming-Yong WANG ; Fan-Ping WANG ; Jing-Jing ZHAI ; Jun-Peng LI ; Na ZHANG ; Hao-Dian LI ; Kang GUO ; Shi-Jun SONG ; Hai-Chuan YU ; Wen-Xia ZHAO ; Meng-Jie LI
Journal of Experimental Hematology 2015;23(2):517-521
OBJECTIVETo investigate the effects of mannan-binding lectin (MBL) on the maturation and cytokine secretion of human dendritic cells (DC) induced by Candida albicans (C. albicans).
METHODSThe plastic-adherent mononuclear cells were prepared from the blood of healthy adult volunteers. The human peripheral blood mononuclear cells-derived dendritic cells (MNC-DC) were induced by 5-day-culture in medium supplemented with rhGM-CSF and rhIL-4, and then cultured for 2 days in presence or absence of C. albicans at varying concentration of human MBL ranging from 1 to 20 mg/L. DC's shape and characters were observed under inverted microscopy, the expression of CD83 and CD86 on DC was analyzed by FACS. The levels of TNF-α and IL-6 were detected by ELISA. FACS also was used to investigate the interaction of MBL with immature DC(imDC) and C. albicans. Western blot was used to detect C. albicans-induced IκBα phosphorylation and p65/NF-κB translocation in DC.
RESULTSMBL at higher concentrations (10-20 mg/L) down-regulated the expression of CD83 and CD86 on the monocyte-derived dentritic cells(MoDC) induced by C. albicans, and inhibited the production of TNF-α and IL-6 induced by C. albicans. FACS showed that MBL could not only bind to C. albicans but also bind to imDCs in a Ca2+-dependent manner. Western blot showed that MBL could decrease the phosphorylation of IκBα and the nuclear translocation of p65/ NF-κB.
CONCLUSIONMBL may inhibit TNF-α and IL-6 production induced by C. albicans in DC through NF-κB signaling pathways, suggesting that MBL can play some roles in the regulation of C. albicans-induced immune response.
Candida albicans ; Cell Differentiation ; Cytokines ; Dendritic Cells ; Humans ; Mannose-Binding Lectin ; NF-kappa B ; Protein Transport