1.Research progress of molecular-targeted ultrasonic imaging in angiogenesis and heterogeneity of tumors
Yingjia LI ; Li YANG ; Ge WEN
Chinese Journal of Medical Imaging Technology 2009;25(10):1899-1902
Tumor blood vessels are characterized with insufficiency and distortion. Endothelial molecules of neovascularization and the heterogeneity of microvasculature in tumor interstitial are the basis for molecular-targeted ultrasonic evaluation of tumor angiogenesis, and has become focus in recent years. Research progresses of molecular-targeted ultrasonic imaging in angiogenesis and heterogeneity of tumors were reviewed in this article.
2.Study on the hemodynamic changes in benign and malignant breast tumors by ultrasound contrast quantitative analysis
Yingjia LI ; Ge WEN ; Jie HE
Chinese Journal of Ultrasonography 2009;18(6):517-520
Objective To compare the differences in histological morphology and hemodynamics between benign and malignant tumor, as well as differences between the different perfusional regions in tumors of the same type. Methods Thirty cases with malignant breast carcinoma and 30 cases with fibroadenoma were inspected by contrast enhanced ultrasound with time-intensity quantitive analysis. Perfusion index like peak intensity(PI), area under the curve(AUC), time to peak(TTP) and wash-out time (WOT) were collected both inside and at the margin of focus. The ultrastructures of new microvessesl were observed routinely with microscopy and transmission electron microscopy. Results The curve of malignant tumor group was characterized as ascend rapidly and drop slowly while the benign group presented as ascend slowly and drop rapidly. The AUC and WOT of malignant tumor group were significantly higher than that of benign group,while the PI and TTP had no statistical difference. In malignant tumor group,PI, AUC and WOT of the margin of focus were significantly higher than that of inside region of focus,while TTP was the opposite. However,there was no statistical differences between the perfusion parameter inside and outside of focus in benign group. The ultrastructures of newly built blood vessels in malignant group had strong ability to split,which was different from normal endothelium cells but was similar to other tumor foci. The margin of foci were characterized with dilatating and distorting vessels,and the center of the foci were occupied by narrow,occlusive new vessels and contracted anamorphic endothelium cells and pericytes. Rich microvessel areas located in the margin of foci. Conclusions The perfusion pattern,modality of time-intensity curve, mean perfusion parameter and variance of regional perfusion parameters are valuable diagnostic basis in discriminating benign and malignant breast tumor. The density,modality,distribution,structure and function of new microvessels in tumor focus are also keystone factors when tumors are assessed by means of imaging.
3.Progress in Castor Biotechnology
Bao-Ge ZHU ; Wen-Bin LI ;
China Biotechnology 2006;0(10):-
Castor(Ricinus communis L.),a high energy-stored and industrial raw-material plant species,is of great value in its application.Progresses in castor biotechnology were summarized in terms of tissue culture and genetic transformation.The results of studies on tissue culture indicated that application of TDZ(thiadiazuron) gave the maximum rate of shoot proliferation in various exogenous hormones when embryo axis was used as explant.On this ground,the in vitro regeneration system was established.The studies on castor genetic transformation showed that the Agrobaterium-mediated method was the most suitable one in different castor transformation methods.The hygromycin is suitable to selection of castor transfomants while castor embryo axis is insensitive to kanamycin.The possibilities of breeding of new castor varieties and improvement of castor production via biotechnology were also discussed.
4.Effects of sevoflurane anesthesia on sleep architecture of rats
Wen LI ; Long LI ; Ge SONG ; Hailong DONG
Chinese Journal of Anesthesiology 2017;37(2):159-162
Objective To evaluate the effects of sevoflurane anesthesia on the sleep architecture of rats.Methods Sixteen pathogen-free healthy male Sprague-Dawley rats,aged 10-12 weeks,weighing 300-350 g,were divided into 2 groups (n=8 each) using a random number table:control group (group C) and sevoflurane group (group S).Each rat was implanted with a transmitter for recording electromyogram and electroencephalogram via telemetry.The rats were exposed to 2.4% sevoflurane and pure oxygen 1.5 L/min for 5.5 h followed by 0.5 h washout with pure oxygen in group S,and the rats were exposed to pure oxygen 1.5 L/min for 6 h in group C.Then the rats were taken into the sleep monitoring box,and the 24 h after anesthesia was divided into 4 time periods according to the circadian rhythm:L1 (14:00-20:00),D1 (20:00-02:00),D2 (02:00-08:00) and L2 (08:00-14:00).The total time spent on wakefulness,on non-rapid eye movement (NREM) sleep and on REM sleep,the number of wakefulness,NREM sleep and REM sleep,and the time spent on wakefulness,on NREM sleep and on REM sleep during each time period were recorded using Version 3.0 Neurosore software.Results Compared with group C,the total time spent on wakefulness was significantly shortened,the total time spent on REM sleep was prolonged,the number of NREM sleep was increased,the time spent on REM sleep in L1 and D1 time periods was prolonged,the time spent on wakefulness in D2 time period was shortened,the time spent on NREM sleep was prolonged (P<0.05),and no significant change was found in the total time spent on NREM sleep or the number of REM sleep and wakefulness in group S (P>0.05).Conclusion Sevoflurane anesthesia can change the stability of sleep architecture,increase REM sleep and reduce wakefulness in rats.
5.Predictive value of ABCD2 score for vertebrobasilar artery stenosis in patients with posterior circulation transient ischemic attack:a retrospective case series study
Min LI ; Yun LI ; Liang GE ; Wen SUN ; Xinfeng LIU
International Journal of Cerebrovascular Diseases 2012;(11):829-833
Objective To investigate the correlation between ABCD2 scores and vertebrobasilar artery stenosis in patients with posterior circulation transient ischemic attack (TIA).Methods The patients with posterior circulation TIA who performed cerebral angiography were enrolled in the study,and their ABCD2 scores and imaging data were analyzed.Results A total of 108 patients with posterior circulation TIA were enrolled.There were significant differences in the main trunk stenosis degree of vertebrobasilar artery with different ABCD2 scores in patients with posterior circulation TIA (P =0.005).The overall degree of stenosis increased with the increase of ABCD2 score (P =0.000).After adjustment for other vascular risk factors,ABCD2 score had significant predictive ability for whether had posterior circulation stenosis (odds ratio [OR]1.771,95% confidence interval [CI] 1.234-2.542; P =0.002) and moderate to severe stenosis (OR 2.083,95% CI 1.336-3.176; P =0.001).Its receiver operating characteristic area under the curve was 0.693(95% CI 0.586-0.800) and 0.755 (95% CI 0.661-0.849) respectively.When the ABCD2 score was ≥3,the sensitivity and specificity of predicting posterior circulation stenosis were 62.6% (95% CI 53.4%-71.7%) and 70.6% (95% CI62.0%-79.1%) respectively,and the sensitivity and specificity of predicting posterior circulation stenosis > 50% were 78.7% (95% CI 70.9%-86.4%) and 67.5% (95% CI58.7%-76.3%) respectively.Conclusions In patients with posterior circulation TIA,the stenosis degree of vertebrobasilar arterial trunk increases with the increase of ABCD2 score.In a certain extent,ABCD2 score may predict whether the main trunk of the posterior circulation has stenosis and whether has moderate to severe stenosis.
6.Epstein-Barr virus positive anaplastic-like plasmacytoma: report of a case.
Wen-sheng LI ; Xiao-ge ZHOU ; Jian-lan XIE
Chinese Journal of Pathology 2013;42(3):203-204
ADP-ribosyl Cyclase 1
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metabolism
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Aged
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Epstein-Barr Virus Infections
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Herpesvirus 4, Human
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isolation & purification
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Humans
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Interferon Regulatory Factors
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metabolism
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Ki-67 Antigen
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metabolism
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Male
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Nasal Cavity
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Nose Neoplasms
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metabolism
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pathology
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surgery
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virology
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Plasmacytoma
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metabolism
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pathology
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surgery
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virology
7.Analysis on Cost-Effect of Three TCM Injections for the Treatment of Stroke
Hui WANG ; Ting LI ; Wen ZUO ; Yan GE
Chinese Journal of Information on Traditional Chinese Medicine 2015;(3):115-116
Objective To compare the clinical efficacy and carry out analysis on pharmacoeconomic cost-effect of three therapeutic schemes in the treatment for stroke. Methods Through a retrospective survey method, 115 stroke patients, based on different treatment methods, were divided into 3 groups:Xuesaitong group (A), carthamin yellow group (B), and Xueshuantong group (C). An analysis on pharmacoeconomic cost-effect was conducted. Results The costs of three therapeutic schemes were 1030.4 yuan, 1876 yuan, and 1545.6 yuan, respectively. The total effective rates of stroke patients in groups A, B and C were 85.37%, 88.57% and 90.04%, respectively. The cost-effect ratios of groups A, B and C were 12.07, 21.18, and 17.17. The added cost-effect ratios of groups B and C compared with group A were 264.25 and 110.32, respectively. Conclusion Xueshuantong Injection has more pharmacoeconomic advantage than Xuesaitong and carthamin yellow Injections in treating stroke.
8.Update on relation between follicular helper T cells and lymphoma.
Chinese Journal of Pathology 2013;42(9):634-637
Biomarkers, Tumor
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metabolism
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Humans
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Immunoblastic Lymphadenopathy
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metabolism
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pathology
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Lymphoma
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genetics
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metabolism
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pathology
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Lymphoma, Follicular
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genetics
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metabolism
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pathology
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Lymphoma, T-Cell, Peripheral
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genetics
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metabolism
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pathology
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Signal Transduction
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Skin Neoplasms
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metabolism
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pathology
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T-Lymphocytes, Helper-Inducer
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metabolism
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pathology
10.Localization of dopamine- and cAMP-regulated phosphoprotein in mouse kidney
Hua ZHAO ; Ping GE ; Jianping WANG ; Haiying WEN ; Hengjin LI
Journal of Third Military Medical University 2003;0(13):-
Objective To investigate the expression and localization of dopamine- and cAMP-regulated phosphoprotein of 32 000 (DARPP-32) in mouse kidney tissue. Methods The cellular localization of DARPP-32 in mouse kidney tissue was detected by immunoblot and immunohistochemistry. Results DARPP-32-like immunoreactivity was detected in the medullary thick ascending limb of the loop of Henle, the cortical proximal convoluted tubule, and collecting ducts in medullary rays. The renal tubules were enriched of Na+, K+-ATPase for sodium reabsorption. Conclusion The participation of DARPP-32 is a likely crucial step of the signal-transduction pathway of dopamine regulation on sodium reabsorption in renal tubule cells.