Objective To study the MR imaging features of intracranial subependymomas and to improve the knowledge more about it. Methods The imaging features of intracranial subependymomas in 17 cases proved pathologically were retrospectively analyzed, among which 11 cases were male and 6 cases were female, 16 cases had MR images and 1 case only had CT images.Results MR appearances of intracranial subependymomas were as follows :16 cases were all hyperintense relative to the normal grey matter on T2-weighted images and hypointense or isointense on T1-weighted images. 12 cases showed no enhancement or minimal enhancement after Gd-DTPA administration . 9 of 16 cases were located in lateral ventricle, 2 were in septum pellucidum, and the rest 5 were in fourth ventricle ,third ventricle ,prepontine cistern, right CPA and chiasmatic cistern respectively. In one case , CT showed isodensity and was enhanced in CE-CT,the lesion was situated in fourth ventricle. In all 17 cases, 11 were accompanied with microcystic structures. Conclusion Intracranial subependymomas are best delineated by MR imaging. The diagnosis of intracranial subependymomas should be considered when above findings are confronted.

Objective:To compare 4 polyphyllins contents in Paris polyphylla planted under walnut forest in different areas of Yun-nan Yangbi. Methods:Paris polyphylla samples planted under walnut forest were collected in different areas of Yunnan Yangbi, and according to the methods described in Chinese Pharmacopoeia (2015 edition), the contents were determined. Results:Yunnan Yangbi different planted in Paris polyphylla four polyphyllins content of each sample there were significant differences between samples were cultivated in imitation of the wild forest, but the cultivation of varying lengths of time, the growth period of 4 to 5 years in Paris polyphylla four polyphyllins content was significantly higher than that of 3 born in Paris polyphylla. Conclusion: Four kinds of Paris polyphylla saponin content of the samples have significant differences with the Paris polyphylla growth of ecological environment, growth period and the same species living on different populations, the genetic background.

Objective To investigate the effects of different doses of dexmedetomidine used in SEP and MEP monitoring in patients undergoing neurosurgery. Methods Eighty patients undergoing neurosurgery receiving SEP and MEP monitoring were randomly divided into 4 groups(n = 20 each):group C,group D1,group D2 and group D3. In groups D1 ,D2 and D3 ,dexmedetomidine 0.5 μg/kg was infused over 10 minutes before anesthesia induction,and then was infused at a rate of 0.1,0.3 and 0.5μg/(kg·h)respectively toward the end of operation. Group C received the equal volume of normal saline. HR ,MAP and BIS were recorded at admission to the operating room(T1),skin incision(T2),when the muscle relaxants were stopped(T3)and 50 minutes later(T4). The current intensity and the time when first MEP was induced after muscle relaxant was stopped ,the amplitudes and latencies of SEP(N20-P25,N20)and MEP on thenar muscle at T4,the total consumption of propofol,and development of adverse affects were also recorded. Results Compared with groups C and D1,HR and MAP were decreased at T2-T4 in groups D2 and D3(P<0.05). The amount of propofol consumed were lower in groups D2 and D3 than in groups C and D1(P < 0.05). The current intensity inducing MEP was lower and the amplitude of MEP at T4 was higher in group D2 than in groups C,D1 and D3,and the situation was same in group D3 than in group C (P<0.05). No significant change was found in the other parameters in groups C ,D1 ,D2 and D3(P>0.05). Conclusion Dexmedetomidine infused at 0.3 μg/(kg · h) after infusion of a loading dose of 0.5 μg/kg could improve monitoring quality of MEP through reducing the amount of propofol consumed ,have less inhibition on MEP than other groups,have no obvious effects on SEP,andmaintain hemodynamic stability.

Allergens ; immunology ; Animals ; Asthma ; etiology ; Enzyme Activation ; Lung ; pathology ; Matrix Metalloproteinases ; physiology ; Mice ; Respiratory Syncytial Virus Infections ; enzymology ; pathology

Objective Many studies have proved that the ABCD 2 score can be used to predict early stroke risk after transient ischemic attack ( TIA) , but few reports are seen on its predictive value for early stroke risk after TIA in different types of circulation . This article aimed to evaluate the ABCD 2 score in predicting early stroke risk following anterior or posterior circulation TIA . Methods Patients with definitely diagnosed TIA consecutively included in Nanjing Stroke Registration System from September 2011 to December 2012 were classified into anterior and posterior circulation TIAs .Various risk factors were collected and a 90-day follow-up was conduc-ted.The ABCD2 score was obtained from each patient and its predictive value assessed using the receiver operating characteristic curve. Results A total of 204 patients were analyzed in the study , including 59 (28.9%) females, 143 with anterior and 61 with posterior circulation TIA, aged 63.7 ±11.2 years.There were no statistically significant differences in the incidence rate of stroke within 90 days between the anterior and posterior circulation TIAs (11.2% vs 9.8%, P=0.776).The mean ABCD2 score was re-markably higher in the anterior than in the posterior circulation TIA (3.7 ±1.5 vs 2.5 ±1.3, P<0.001).The area under the curve (AUC) in predicting stroke risk was 0.711 for the former (95%CI:0.575-0.847, P=0.006) and 0.555 for the latter (0.298-0.820, P=0.637). Conclusion The ABCD2 score can predict early stroke risk in anterior but not in posterior circulation TIA .

Objective To discuss the effect and clinical value of serum sialic acid (SA) and lactadherin(MFG-E8) in the coronary atherosclerotic heart disease(CHD).Methods Selected 295 patients who visited to Wuhan University Renmin's Hospital cardiovascular medicine (from January to June, 2014), and then according to the results of coronary angiography divide the subjects into control group (n =78, the result of coronary angiography completely normal)and coronary heart disease group(n =217, the results of coronary angiography showed that one major coronary stenosis was more than 30％).Detected their serum level of lipid, SA, MFG-E8, hs-CRP index.At last, analyze the differences of SA and MFG-E8 between these groups, and the correlation with the severity of coronary stenosis (symbolize with Gensini score), use the binary logistic regression analysis to research the relationship among the serum level of SA, MFG-E8 and the risk of CHD.Results Compared with the control group, the patients with CHD have a higher level of serum SA and a lower level of MFG-E8 (U =4 402.5, 2 736.0 respectively,P ＜0.001);the AMI group has a higher level of serum SA than the StA and UA groups (U =1 329.0,957.5 respectively, P ＜0.001);the AMI group has a lower level of serum MFG-E8 than the StA groups (U =1517.0, P ＜ 0.001).The spearman correlation analysis showed that the serum SA has a positively correlation with the level of TG, hsCRP and Gensini score, and has a negatively correlated with the level of MFG-E8 (r =0.214, 0.462, 0.378,-0.322 respectively,P ＜0.01);the MFG-E8 has a negatively correlated with the level of TG, hsCRP and Gensini score (r =-0.18,-0.425,-0.564 respectively, P ＜0.01).Binary logistic regression analysis showed that there was a positive correlation between the serum SA and the risk of CHD (OD =4.782, 5.319, 4.179 3.832, 3.648 respectively, P ＜0.001), and the was a negative correlation between the serum MFG-E8 and the risk of CHD (OD =O.118, 0.112, 0.127, 0.135, 0.134 respectively, P ＜ 0.001).Conclusion With the serum SA rised and the MFG-E8 reduced, the risk of coronary heart disease was higher, combined detection these two indexes may be able to diagnose the CHD and assess the degree of stenosis and the risk of CHD.

Objective To observe the effects of Xinshuaiheji (XSHJ) on cardiac function, plasma angiotensin Ⅱ (AngⅡ) and histomorphology in rats with heart failure after acute myocardial infarction. Methods A model of heart failure (HF) induced by myocardial infarction (MI) in rats was made, 10 days after MI, rats were treated for 4 weeks with bidist Water, Captopril, high dosage of XSHJ, low dosage of XSHJ. The effects of Xinshuaiheji on cardiac function (stroke volume, SV, cardiac output, CO, cardiac index, CI ) and AngⅡ were observed. We also observed and compared the changes of heart weight/body weight ratio (HW/BW), ratio of ventricular wall thinning in MI. Results After treatment with XSHJ, the cardiac function (SV, CO, CI) of HF rats improved (P

Objective To investigate the effects of lipid microspheres prostaglandin E1(Lipo PGE1) on subarachnoid hemorrhage(SAH).Methods Ninty-three patients with SAH were randomly divided into control group(treated with nomal drugs) and Lipo PGE1 treatment group(treated with nomal drugs and Lipo PGE1).Changes in neuroimaging,biochemical indexes and incidence of cerebral vasospasm were measured. Results A lower incidence rate of cerebral vasospasm was observed in Lipo PGE1 treatment group(P0.05).The levels of vWF and GMP-140 were significant lower in the Lipo PGE1 treatment group than those in the control group after treatment for 3 and 7 d(P

Objective To investigate the changes of somatosensory evoked potential(SEP)in subarachnoid hemorrhage(SAH)and in-fluence from lipid microspheres prostaglandin E1(Lipo PGE1). Methods Ninty-three patients with SAH were randomly divided into control group(treated with nomal drugs) and Lipo PGE1 treatment group(treated with nomal drugs and Lipo PGE1).Clinical outcomes and changes of SEP before and after the treatment were observed. Results After the treatment,the latency of N20 wave was prolonged in both groups(P

Objective To investigate the suppression effect of RNA interference(RNAi) on the expression of epidermal growth factor receptor(EGFR) of ovarian cancer SKOV3 cell line. Methods SKOV3 cells were transfected with synthetic EGFR sequence-specific dsRNA by lipofectamin 2000.The expression level of EGFR mRNA and protein were detected by RT-PCR and Western blotting,respectively.The proliferation of transfected SKOV3 cells was detected by WST-1 cell proliferation assay.The abilities of adhesion,migration and invasion were detected by cell adhesion assay,cell migration assay and Matrigel invasion assay,respectively. Results The suppression rates on EGFR mRNA and protein by sequence-specific dsRNA-EGFR were 73.65% and 58.94%,respectively.The cell proliferation was inhibited by 22.54%,35.76% and 31.07%,respectively at 24,48 and 72 h after transfection.The adhesion of sequence-specific dsRNA transfected SKOV3 cells for 30 min and 90 min were reduced by 12.11% and 18.66%,respectively.The migration and invasion of SKOV3 cells were decreased by 25.47% and 22.08%,respectively. Conclusion The down-regulation of EGFR expression of ovarian cancer by sequence-specific dsRNA can lead to the suppression of proliferation,adhesion,migration and invasion of ovarian cancer cells.