Breast Neoplasms ; classification ; pathology ; Carcinoma, Ductal, Breast ; classification ; Carcinoma, Intraductal, Noninfiltrating ; classification ; Carcinoma, Papillary ; classification ; Classification ; methods ; Female ; Humans ; World Health Organization

The relationship between probiotics and host intestinal immune system was reviewed. Then, as a new immunomodulator, the mechanisms and clinical uses of probiotics were discussed.

A case of Schwannoma in the submandibular gland was reported which had been misdiagnosed as pleomorphic adenoma before operation. The tumor was originated from lingual nerve which in turn invaded the Schwann membrane near the submandibular ganglion.
Adenoma, Pleomorphic ; Diagnostic Errors ; Humans ; Neurilemmoma ; diagnosis ; Submandibular Gland ; pathology ; Submandibular Gland Neoplasms ; diagnosis

Objective To study hematotoxic effect of formaldehyde in mice.Methods Sixty healthy KM mice were randomly divided into a control group and three formaldehyde treated groups named a low level group,a moderate level group and high level group.Themice in the experimental groups were exposed to formaldehyde by intraperitoneal injection,once a day,for three weeks at the dose of 0.2,2.0 and 20 mg/kg bw respectively.Five mice were sacrificed at the 1st,2nd,3rd week of formaldehyde treatment and the blood samples were collected.The red blood cell counts,white blood cell counts and the hemoglobin content in the blood in mice were determined.Results During the experimental period,the body weight of mice in low level group at the 1st and 3rd week decreased significantly compared with the control group.The organ coefficient of the kidney in the moderate level group,high level group at the 1st week were higher than that of control group.The organ coefficient of the spleen in moderate level group at the 2nd week,in all three formaldehyde treated groups at the 3rd week was lower than that of control group.The organ coefficient of the heart in high level group at the 3rd week also decreased markedly in comparison to that of control group.The hemoglobin content in high level group at the 3rd week was lower than that of the control group.The red blood cell counts in three experiment groups at the 1st week and in high level group at the 2nd week decreased markedly compared with the control group.The white blood cell counts were seen much lower in low level group at the first two weeks,in moderate level group at the 1st week and in high level group in the experimental period compared with the control group.Conclusion Formaldehyde exposure has some hematotoxic effects in mice.

Objective To evaluate the curative effect and indications of medical ozone for the treatment of lumbar disc herniation.Methods 10~20 ml(35~45 ug/ml) medical ozone was injected into the lumbar disc percutaneously each case. The patients were followed up for 6~12 months. Results 60 cases (66 lumbar discs) with lumbar disc herniation were treated with the injection of medical ozone. The total effective rate was 96.67% , excellent and good rate was 76%.The efficacy in herniation to be 30% of vertebral canal diameter and lumbardisc bulging group. Conclusion The treatment of lumbar disc herniation with medical ozone is simple,effective and no complications.

This study aimed to investigate the therapeutic effect and possible mechanism of carboxyamidotriazole (CAI) on imiquimod (IMQ)-induced psoriasis-like mice model and M5 (IL-1α, IL-17A, IL-22, TNF-α and oncostatin M)-induced keratinocytes model of psoriasis. The severity of psoriasis-like skin lesion in mice was evaluated by psoriasis area and severity index (PASI) score. The histopathological changes of skin were examined by hematoxylin-eosin staining and Baker score was calculated. The levels of pro-inflammatory cytokines in skin were measured by enzyme-linked immunosorbent assay. Transcriptome sequencing technique was used to analyze differentially expressed genes (DEGs) and real-time quantitative PCR (qPCR) was used to detect mRNA expressions. The animal experiments conducted in this study were approved by the Institutional Animal Care Use & Welfare Committee of Institute of Basic Medical Sciences, Chinese Academy of Medical Science (grant No. ACUC-A02-2022-115). The results showed that CAI significantly improved the severity of psoriasis-like lesion, reduced PASI score, attenuated pathological changes, decreased Baker score and inhibited the levels of IL-1β, IL-6, IL-17A, IL-23 and TNF-α in skin of IMQ-induced mice. Transcriptome sequencing analysis revealed that regulating keratinocytes and their mediated keratinization might be involved in the mechanism of CAI. Further qPCR study validated that CAI down-regulated the mRNA expression of DEG S100a7. Moreover, the keratinocyte model of psoriasis was established by stimulating HaCaT cells by M5. It was shown that CAI decreased the mRNA expression levels of S100a7, Il1β, Il6, Il17, Il23 and Ccl20, which were up-regulated by M5 stimulation. In conclusion, CAI might have a good therapeutic efficacy on psoriasis, and its mechanism was related to regulate the function of keratinocytes and downregulate cytokines and S100a7.

Blindness ; epidemiology ; etiology ; prevention & control ; China ; epidemiology ; Humans ; Vision, Low ; epidemiology ; etiology ; prevention & control

Partial immunoactivities of peptidoglycan(PG)isolated from lactic acid bacteria were investigated.PG isolated from strain Z8 and Z17 of lactic acid bacteria respectively,had similar immunoactivities.The phagocytic function of M?(macrophage)increased markedly and serum lysozyme activity was significantly enhanced by injection of PG-extracts on mice.Investigation of immuno-enhancing effects of PG on vaccine of Newcastle disease in chickens showed that the hemagglutination inhibition levels of PG were higher than that of the control and the level was maintained for a longer time as compared to the control.

Objective To study the impact of local fractionated irradiation on lung metastasis in H22-bearing mice, and to explore its mechanism involved. Methods Subcutaneous transplantation tumor model bearing with H22 was established. Mice were divided into three groups as healthy control, tumor control and irradiation groups. The size of subcutaneous tumors was measured and lung metastasis was observed. The expressions of PCNA, VEGF and MVD were detected immunohistochemically. The plasma levels of CD4 and CD8 were determined by using flow cytometry. Results The tumor size in irradiated group was smaller than that in tumor control group. The tumor inhibition rate in irradiated group was 30%.Lung metastasis in irradiated group was more severe than that in tumor control group (x2 = 8.31,4.48,9. 60, P＜ 0.05 ). The expressions of PCNA, VEGF and MVD in two groups were statistically different( t =23.78,-2.47, -6.43, P ＜ 0. 05). The levels of CD4 and CD8 in irradiated group were statistically different compared to healthy control group ( t = 4. 72 and 3.31, P＜ 0.05 ). Conclusions For the H22 model, radiation might inhibit the local transplantation tumor, but increase the risk of lung metastasis.

Carcinoma, Papillary ; diagnosis ; pathology ; Female ; Humans ; Jugular Veins ; pathology ; Male ; Middle Aged ; Neoplastic Cells, Circulating ; Retrospective Studies ; Thyroid Neoplasms ; diagnosis ; pathology