OBJECTIVETo compare the content of the active ingredient resveratrol in Smilax china from different habitats.

METHODThe ingredients of samples from different habitats in China were analyzed for resveratrol in S. china by HPLC.

RESULTThere was a significant differences in resveratrol content between the samples.

CONCLUSIONResveratrol content in the sample from Qianshan (Anhui province) is obvious higher than those from other habitats.

Altitude ; China ; Chromatography, High Pressure Liquid ; methods ; Ecosystem ; Plants, Medicinal ; chemistry ; Quality Control ; Reproducibility of Results ; Rhizome ; chemistry ; Smilax ; chemistry ; Stilbenes ; analysis

Stirred culture offers a number of advantages over static systems as it maintains a stable, homogeneous culture environment and is easy to scale-up. This paper focused on the development and application of stirred tank bioreactor to culture hematopoietic cells. Preliminary study of stirred culture of hematopoietic cells was carried out in cord blood mononuclear cells culture in spinner flask. The results showed that the amplification rates of total cell, CFU-GM and BFU-E, with the exception of CFU-Mk, were greater in spinner flask than T-flask. The number of total cells increased 20 fold after 14 days incubation in spinner flask. The amplification rates of CFU-GM, CFU-Mk and BFU-E reached maximum at 10th day, 10th day and 7th day respectively, and the maximal amplification rates were 9.2-fold, 5.5-fold and 2.4-fold respectively, whereas the rate of CD34+ cells in spinner flask was (6.7 +/- 4.0)-fold at day 10. These results indicated that the stirred culture system is better than the static culture systems for hematopoietic cell proliferation. The biocompatibility of cord blood MNC to different types of materials used in bioreactors was also tested. The results showed that glass, stainless steel 316L and polytetraflouroethylene (PTFE) supported the growth of hematopoietic cells well. A higher cell density was reached in stirred bioreactors with controlled pH and DO than static culture. These findings suggested that the controlled large-scale culture could be used to overcome the clinical shortage of hematopoietic cells.
Antigens, CD34 ; metabolism ; Bioreactors ; Cell Culture Techniques ; instrumentation ; methods ; Erythroid Precursor Cells ; cytology ; Fetal Blood ; cytology ; Granulocyte-Macrophage Progenitor Cells ; cytology ; Humans ; Polytetrafluoroethylene ; Stainless Steel

OBJECTIVE: To investigate the current status of quality of life( QOL) in patients with occupational pneumoconiosis,and explore the effects of the social demographic data and disease-related information on their QOL.METHODS: By the convenience sampling method,144 male patients with occupational pneumoconiosis were selected. The St. George's Respiratory Questionnaire( SGRQ) was used in the study and the stepwise regression analysis method was used to analyze the influencing factors of QOL in these patients. RESULTS: The SGRQ total score was( 60. 0 ± 18. 0),respiratory symptom dimension score was( 61. 2 ± 19. 7),limitation of motion dimension score was( 64. 0 ± 21. 5) and the disease influence dimension score was( 57. 7 ± 19. 5). The QOL of 52. 08%( 75 /144) of the patients were below the average level. The stage of pneumoconiosis was positively correlated with SGRQ total score and the above three dimension scores( P < 0. 01). The length of disease was negatively correlated with respiratory symptom dimension score( P < 0. 05);the educational level was negatively correlated with limitation of motion dimension score( P < 0. 01); the lung function was positively correlated with limitation of motion dimension score( P < 0. 05). CONCLUSION: The QOL of patients with pneumoconiosis was below the average level. The stage of pneumoconiosis,length of diagnosis,the educational level and lung function are the important factors affecting the QOL of these patients.

OBJECTIVETo explore the methods of diagnosis and surgical treatment for patients with back pain originating from endplate.

METHODSAll patients received examinations of radiography, CT, and MR imaging. Pain level of disc was decided by discography in each patient. The principal outcome judgment were pain and disability, and the efficacy of surgical treatment was assessed by visual analog scale (VAS) for pain, and the Oswestry disability index (ODI) for functional recovery.

RESULTSAll patients with a diagnosis of back pain originating from endplate according to discography were treated with anterior or posterior fusion surgery. The mean follow-up period was three years and five months (from 2 to 6 years). Among of the 21 patients, 20 (95%) reported a disappearance or marked alleviation of low back pain, and experienced a definite improvement in physical function. A statistically significant and clinically meaningful improvement on the ODI and the VAS scores were obtained in the patients with chronic low back pain originating from endplate (P = 0.0001) after treatment.

CONCLUSIONSThe study suggests that the discography and fusion surgery may be very effective methods for the diagnosis and treatment of chronic back pain originating from endplate respectively.

Adult ; Female ; Follow-Up Studies ; Humans ; Low Back Pain ; diagnosis ; etiology ; surgery ; Male ; Middle Aged ; Spinal Fusion ; methods ; Spinal Injuries ; complications ; Treatment Outcome

Purpose To study the expression and the clinical significance of β3GnT8,MMP-2,PCNA in gastric cancer tissue. Methods The histological chips of paraffin specimens of gastric cancer were prepared,and immunohistochemical methods were used to detect the expression of β3GnT8,MMP-2 and PCNA in gastric cancer and adjacent tissue. Results The expression of β3GnT8 and MMP-2,PCNA in gastric cancer was higher than that in adjacent tissue (P = 0. 001) . The expression of β3GnT8 and MMP-2 was significantly positively correlated with the clinical stage (P = 0. 001) ,depth of invasion (P = 0. 011) and lymph node metastasis (P = 0. 003) of gastric cancer. The expression of β3GnT8 was significantly positively correlated with that of MMP-2 (r = 0. 703,P = 0. 001) and PCNA (r = 0. 231,P = 0. 024) . The overall survival time of the β3GnT8 positive expression group was significantly shorter than that of the negative expression group(χ2 = 3. 957,P = 0. 047) . Conclusion The expression of β3GnT8 is increased in gastric cancer. β3GnT8 can promote the invasion and metastasis of gastric cancer and is associated with poor prognosis in patients with gastric cancer.

OBJECTIVETo observe the clinical effect of Shengxueling (SXL) on idiopathic thrombocytopenic purpura (ITP), and study the possible mechanism.

METHODSEighty-six cases of ITP were randomly divided into two groups. The SXL group, 56 patients treated with SXL, a traditinal Chinese medicine and 30 patients administered with prednisone were taken as control. Each group took drugs for 3 months and was under follow-up observation.

RESULTSIn the SXL group, the total effective rate was 85.71%, similar to prednisone 83.33% (P > 0.05) for 3 months, but the total effective rate of SXL (91.07%) were obviously better than that of the control group (53.33%) (P < 0.01) for 6 months and had no obvious adverse reaction. The patients bleeding was alleviated or stopped, the general condition was improved. At the same time, blood platelet count (PLT) was increased, platelet associated immunoglobulin (PAIg) and interleukin-4 (IL-4) were markedly dropped, the level of natural killers cells activity (NKa) increased, the rate of T lymphocyte subsets gradually returned to normal level. Megakaryocyte tended to maturation on bone marrow smear after treatment. All differences above were statistically significant.

CONCLUSIONSXL is an effective and safe medicine for ITP. Its mechanism could regulate cytoimmune, inhibit platelet antibody to reduce the destruction of platelet, increase the number of platelet, promote the division and maturation of megakaryocyte, facilitate the production and release of platelet, lower the fragility of capillary, prevent and cure hemorrhagic tendency.

Adolescent ; Adult ; Blood Platelets ; metabolism ; Bone Marrow ; pathology ; Female ; Glucocorticoids ; therapeutic use ; Hemorrhage ; etiology ; physiopathology ; Humans ; Immunoglobulins ; blood ; Interleukin-4 ; blood ; Killer Cells, Natural ; pathology ; Lymphocyte Count ; Male ; Medicine, Chinese Traditional ; Middle Aged ; Phytotherapy ; adverse effects ; Plant Preparations ; adverse effects ; therapeutic use ; Platelet Count ; Prednisone ; therapeutic use ; Purpura, Thrombocytopenic, Idiopathic ; blood ; complications ; pathology ; therapy ; T-Lymphocyte Subsets ; pathology ; Treatment Outcome

OBJECTIVETo establish the role of psychological factors in the etiology and symptomatology of chronic prostatitis / chronic pelvic pain syndrome (CP/CPPS), analyze the influence of the psychological obstacles and other relative factors on the prognosis of CP/CPPS by univariate and multivariate Cox regression analyses, and provide a scientific basis for psychotherapy of the problem.

METHODSA total of 291 CP/CPPS patients and 100 normal controls were investigated in age, education, occupation, character, disease course, NIH chronic prostatitis syndrome index (NIH-CPSI) and leukocyte count in EPS and by self-rating anxiety scale (SAS) and self-rating depression scale (SDS) to establish the psychological factors related with CP/CPPS. Then, all the CP/CPPS patients were treated with the same method and followed up for 6 weeks. Based on the therapeutic results, the influence of psychological and other relative factors on the prognosis of CP/CPPS was analyzed with univariate and multivariate Cox regression.

RESULTSAll together 258 valid questionnaires were collected from the patients and 87 from the normal controls. Of the 258 CP/CPPS patients, the mean scores on SAS and SDS were 42.8 +/- 11.43 and 48.15 +/- 11.49 respectively, both significantly higher than those of the controls (32.12 +/- 9.68 and 35.12 +/- 10.81) (P < 0.01). The rates of anxiety, depression and anxiety and/or depression in the CP/CPPS group were 25.97, 21.71 and 34.50 % respectively, all significantly higher than in the control group (P < 0.01). The rate of introversion was significantly higher while that of extroversion significantly lower in the former than in the latter (P < 0.01). The total effectiveness rate of treatment was 70.54 % in the CP/CPPS patients. Univariate and multivariate analyses with Cox regression revealed that anxiety, depression and disease course were the definite factors that negatively affected the prognosis of CP/CPPS, while the other factors, such as age, CPSI, character and leukocyte count in EPS had no influence.

CONCLUSIONSuch psychological obstacles as anxiety and depression play an important role in the pathogenesis, development and prognosis of CP/CPPS. In the treatment of CP/ CPPS, importance should be attached to the patients'psychological status and proper psychological intervention is sometimes necessary.

Adolescent ; Adult ; Anxiety ; etiology ; psychology ; Chronic Disease ; Depression ; etiology ; psychology ; Humans ; Male ; Middle Aged ; Pelvic Pain ; etiology ; psychology ; Prognosis ; Proportional Hazards Models ; Prostatitis ; complications ; psychology ; Regression Analysis ; Surveys and Questionnaires ; Syndrome ; Young Adult

Objective To investigate the effect of paeoniflorin ( PF) on cytochrome P450 2E1( CYP450 2E1) and lipid peroxidation in L02 cell induced by ethanol.Methods L02 cell model was established by 75 mmol · L-1 ethanol.After treated with paeoniflorin, superoxide dismutase ( SOD) and malondialdehyde ( MDA) levels in the cell culture supernatant were detected by biochemical analysis.The expressions of CYP450 2E1 in L02 cell were observed by RT-PCR and immunocyto-chemistry.Results Compared with the control group, serum SOD levels in model group were significantly decreased, while MDA levels were elevated ( P <0.05 ).The levels of CYP450 2E1 mRNA and protein were significantly increased in model group (P<0.05).However, com-pared with the model group, SOD levels in the cell culture supernatant of PF group were much higher, while MDA levels were decreased, and the levels of CYP450 2E1 mRNA and protein were significantly decreased in PF group ( P<0.05 ).Conclusion The effect of PF on the improve-ment of L02 cell damaged by ethanol and reducing lipid peroxidation could be related with the regulation on the expression of CYP450 2 E1.

Objective To investigate the relation of HBV genotypes to clincal features in children with chronic hepatitis B. Methods The genotypes of serum HBV DNA from 404 children with chronic hepatitis B were determined by PCR using type-specific primers. Results for the 404 children, genotype B in 99(24.5%), genotype C in 255 (70.5%). For the 75 children from south part of China, 29 were of genotype B (38.7%) and44 of genotype C (58.7%). For the 329 children from north part of China, 70 were of genotype B (21.3%) and241 of genotype C (73.3%). There were significant differences between the children from south part and those from north part of China in genotype B and C (P=0.002). Genotype B and C were not significantly correlated congener, age and mother-to-fetus transmission. There was no marked difference in liver injury severity (P=0.4796), serum HBeAg positivity, HBVDNA level, inflammatory degree of liver tissue (P=0.209)and liver fibrosis(P=0.177) between the children with genotype B and those with genotype C. Conclusion In children with HBV infection, genotype C accouts for 70.5% and genotype B for 24.5%. The genotypes are of regional difference in children with HBV infeciton. There are replication and liver pathological change between genotype Band genotype C.

ation were complicated, with the characteristics as the obvious decreasing number of patients, with no food-borne isolates in 2007.