A new class of dendrimer polylysine poly(ethylene glycol)-lipid was designed and synthesized. The cationic polymer liposomes were prepared by the lipid film-extrusion and post-insertion two methods with these dendrimer polylysine poly(ethylene glycol)-lipid and other lipids. The structural properties of obtained cationic polymer liposomes were studied by laser light scattering and fluorescence spectrometer. It was demonstrated that the nano sized liposomes with different density of surface cationic charges can be prepared by either lipid film-extrusion or post-insertion methods, but post-insertion process did not affect drug loading, did not influence drug loading capacity and did not induce liposomal morphology and particle size. The density of positive charge does not affect the size and distribution of different liposomes size and distribution. It was the better choice for manufacture because post-insertion method did not cause early release of drug and size changes. Cell binding experiments show that cationic polymer liposomes, especially dendrimer polymer liposomes had higher local charge density, and therefore have dramatic non specific cell targeting ability.

Objective To explore the effect of valproate(VPA) on body mass index(BMI),leptin and serum lipids in children with epilepsy.Methods (Twenty-)four cases with epilepsy were selected.BMI and serum levels of leptin,total cholesterol(TC),(triacylgly)-cerol(TG),high density lipoprotein-cholesterin(HDL-C),low density hipoprotein-cholesterin(LDL-C) after treated with VPA for 3 months and 6 months were determined.Results The levels of BMI,leptin,TC,TG,LDL-C after treated 3 months were all significantly higher than those of initial(all P0.05).Those in treated 6 months were same as levels of treated 3 months.Only LDL-C level was lower than that of 3 months and same as level of before treatment.Conclusion VPA do have efficacy to cause obesity in children with epilepsy,and most of the cases occur in the first 3 months after VPA treatment.

Objective To investigate the efficacy and safety of autohemotherapy therapy in ASST positive chronic idiopathic ur‐ticaria .Methods One hundred and twenty cases of chronic idiopathic urticaria treated in our department from April ,2012 to Janu‐ary ,2013 were divided into treatment group and control group ,60 cases in each group .Patients in treatment group were given oral ioratadine (10 mg/d) ,supplemented by autohemotherapy ;patients in control group were only given oral ioratadine (10 mg/d) .Both of the two groups were treated with twelve weeks ,then observed the effectiveness and safety of two kinds of treatment .Results The effective rate of treatment group and control group were 68 .33% and 48 .33% respectively ,and there was significant difference between the two groups (P<0 .01) .Conclusion Autohemotherapy combined antihistamine was of high efficiency and safety in the treatment of ASST positive chronic idiopathic urticaria ,while the long‐term curative effect remains to be observed .

Objective To assess the feasibility of using bispectral index (BIS) to guidance of desflurane or sevoflurane anesthesiaMethods Forty ASA Ⅰ-Ⅱ patients were randomly assigned to four groups, After a induction of propofol 2-2?5mg?kg -1 and fentanyl 2ug?kg -1, laryngoscopy and intubation were facilitated with intravenous vecuronium 01mg?kg -1,and anesthesia was maintained with either desflurane or sevoflurane in combination with 60% nitrous oxide In control groups, the anesthesiologists were blinded to the BIS value, and the volatile anesthetics were administered according to their clinical experience In BIS-titrated groups, the volatile anesthetics were titrated to maintain the BIS value at about 60Results During the maintenance period, the SBP in BIS-titrated groups was higher than that in control groups, while SBP fluctuation was less in control groups than that in BIS-titrated groups The requirements of volatile anesthetics were lower in BIS-titrated groups than those in control groupsConclusions In spite of predicting the sedative effect of desflurane or sevoflurane, BIS can not titrate desflurane or sevoflurane anesthesia

Objective To explore the method for simultaneous determination of arsenic and mercury in drinking water. Methods Atomic fluorescence spectrometry was applied for simultaneous determination of arsenic and mercury in drinking water. The best working conditions of the determination, such as the height of flame of atomizer, current of hollow cathode lamp, reducer, acidity and required amount of NaBH 4 were defined in this assay. Results The determination range of arsenic, the correlation coefficient and detection limit for arsenic were 0～40 ng/ml,0 999 5 and 0 10 ng/ml respectively, and were 0～80 ng/ml, 0 999 3 and 0 02 ng/ml for mercury respectively. The precisions and recovery rates of the simultaneous determination of arsenic and mercury in drinking water were 0 94%～5 61% and 94%～110%. Conclusion This method with advantages such as simple, rapid, sensitive, high accuracy and less interference, was suitable for simultaneous determination of arsenic and mercury in drinking water.

?AIM: To compare postoperative anti -inflammation effect and safety between bromfenac sodium eye drops and pranoprofen eye drops in patients after laser epithelial keratomileusis ( LASEK) .? METHODS: In the prospective, randomized and controlled study, 100 patients ( 200 eyes ) undergoing LASEK were randomized into the bromfenac sodium group (100 eyes) and control group (100 eyes).Patients in bromfenac sodium group received bromfenac sodium hydrate ophthalmic solution eye drops twice a day in 3d before surgery and 2wk after surgery, while the patients from the control group were given proanoprofen eye drops 4 times a day in the same period.At 1, 3, 5d, 1 and 3mo after surgery, irritative symptoms grade, duration of irritation, time for corneal epithelial healing, cornel haze, uncorrected visual acuity and intraocular pressure ( IOP ) were observed and compared between the two groups. Quantitative data were analyzed using independent samples t-test and ranked data were statistically analyzed using the Mann-Whiteney rank sun test.?RESULTS:There was no significant difference between two groups in irritative symptoms grade ( P =0.317 ), neither was existed between two groups in uncorrected visual acuity after surgery (P>0.05).There was no statistical significance in the time for corneal epithelial healing between two groups (P=0.551).?CONCLUSION: Bromfenac sodium eye drops ( 1g/L ) can achieve the same therapeutic effect as pranoprofen eye drops after LASEK.

Objective In this study,we investigate the influence on nurses professional ability by the hierarchical level training under the advanced mode.Methods By referring to advanced mode of clinical expertise,combined with impacts including the ability of nurses,seniority factors,professional titles and educational background,1 527 nurses of our hospital were divided into five ranks (including nine grades).We developed training plan,training forms and training purposes aiming at all level of training objectives.In addition,we developed a training effect questionnaire and promotion criteria.All the nurses must be evaluated and compared with the traditional nursing mode,which contained 1 486 nurses as the control.Results Within two years after the start of the experiment in the observation group,1 106 nurses had been promoted.Compared with the traditional platform-based training model,nursing assessment indicators were significantly improved by the hierarchical level of training.Quality control of care services was significantly improved and the score of patient satisfaction was significantly increased.Nurses acceptance of tiered training was improved compared to the control group,published research papers were significantly increased.Conclusions Detailed training on the layer classification of the nurses can improve the ability of nurses including specialist knowledge,interpersonal communication and humanistic care,teaching,scientific research,management and other professional skills,so as to improve nursing quality and work efficiency.

Objective To explore the relationship between CD+4CDhigh25CDlow127 Treg level of peripheral blood and NHL.Methods The level of peripheral blood CD+4CDhigh25CDlow127 Treg of 123 newly diagnosed NHL and 41 healthy adults were test by flow cytometry.Results The average peripheral blood CD+4CDhigh25CDlow127 Treg level were 5.0 ％ (2.7 ％) and 7.2 ％(3.8 ％) in healthy adults and newly diagnosed NHL patients,respectively.The difference between the two groups was statistically significant(Z=-2.816 P=0.001 ).The peripheral blood Treg level was significantly higher in the male NHL patients than that in the female (7.4％ vs 6.1％,Z=- 2.729,P=0.006).The peripheral blood Treg level was significantly higher in elevated LDH patients than that in normal LDH patients(7.8％ vs 7.0％,Z=-2.623 P=0.008).The peripheral blood Treg level was significantly higher in stage Ⅲ-Ⅳ patients than that in stage Ⅰ -Ⅱ patients (7.3％ vs 5.9％,Z=-2.057 P=0.04). There was no significant relationship between peripheral blood Treg level and age, IPI score,extranodal involvement,performance status,bulky disease,pathologic subtype or B symptom (P ＞0.05).Conclusions NHL patients are in an immunosuppresive statue.Patients of male,with inreased LDH level or in stage Ⅲ-Ⅳ have higher peripheral blood CD+4CDhigh25CDlow127 Treg level.

Objective To explore the alterations of protein phosphatase-2A (PP-2A) in lymphocytes in mild cognition impairment (MCI) and Alzheimer's disease (AD).Methods The activity PP-2A of was measured by ~(32)p liquid seintillography for incorporated radioactivity in control group(n=11) , the MCI group(n=11),and the AD group(n=11).The expression of PP-2A was determined by Western blot.Results In the control group,the activity of PP-2A (1.01?0.09) and the expression of PP-2A (0.96?0.07) were high while in the MCI group,the activity of PP-2A (0.71?0.12) and the expression of PP-2A (0.80?0.05) were decreased (both P