AIM: To explore the nursing cooperation highlights of eight osteopetrosis patients underwent optic nerve decompression via transsphenoidal microsurgical approach instead of routine pathway, and to improve the quality of nursing cooperation. ·METHODS: We enrolled 8 cases ( left eye in 3 cases, right eye in 5 cases ) of osteopetrosis patients referred from the Eye Hospital of Wenzhou Medical University during February 2012 to November 2016. Patients received ophthalmic examinations including visual acuity and diagnostic imaging tests in pre-operation and post -operation. All eyes were performed surgical optic nerve decompression through endoscopic approach in assist of image guidance system. We retrospectively analyzed the clinical data and surgical cooperation procedure of these cases and summarized nursing cooperation experience. ·RESULTS:The operations of 8 patients were completed successfully without massive hemorrhage. Mean visual acuity improved from pre-operation (2. 5±2. 1) to post-operation (3. 4±1. 9). Cerebrospinal fluid leakage occurred in 1 patient and was instantly repaired during the operation. We performed the nursing strategy as postural drainage, condition monitoring and conscious assessment intra-and post-operation. ·CONCLUSION: It is the critical for this kind of surgery that both circulating nurse's high-skilled cooperation to the connection and operation of the navigation system, to treat with complication during the surgery, and scrub nurse's sufficient preparation of surgical instruments and consumables, proficient equipment delivery, meticulous management, use and maintenance of equipment.

Objective To investigate the status of food hygiene safety in troop catering units, analyze the major problems, and to improve the service system of food safety in troops.Methods Seventy troop catering units were investigated by means of cross-sectional questionnaire surveys, on-site inspections and rapid detection methods.Results Among the 70 troop catering units, 70.0%were self-managed,and 28.6%were run by civilians.From them, 64.3%of these units were centralized above the regimental level.Five main problems with food safety were found during food processing:uncomplete cleaning and disinfection of the tableware, irrational layout of processing rooms, co-contamination in the processing link, purchase and storage problems of raw materials, and sub-standard internal management of hygiene.44.3%of the catering units had inspection staff, 40.0% were equipped with inspection equipment, and 35.7% had food inspection rooms. Conclusion There are potential hazards to food safety in troop catering units.The implementation of hygienic regulations should be strengthened, and food safety related equipment improved.In addition, staff′s awareness of hygiene should be enhanced, internal supervision should be strengthened, and the mechanism of rewards and punishments should be established.

OBJECTIVETo study the effect of exogenous Ca2+ on protective infection of Pinellia ternata and accumulation of major components under high temperature stress.

METHODThe soilless cultivation experiment was applied, stress resistance index of P. ternata leaves, statistics the rate of lodge P. ternata,the content of oxalate in different places in the plant, the content of total alkaloids, total organic acids and glucosine in P. ternata tubers were measured based on different concentrations of exogenous Ca2+.

RESULTThe test results showed that, at lower concentrations of Ca2+ treatments, the rate of lodge P. ternata was higher than that of the others. With Ca2+ concentration increasing, activities of SOD and POD initially increased and then decreased, however, proline level tended to be down then up. Soluble oxalic acid content was lower than the content of unhandled treatment in P. ternata leaves and tubers; with Ca2+ concentration increasing, soluble oxalic acidl content and yield showed a tendency of decrease after increase in the leaves and tubers. Compared with other treatments, spraying 400 mg x L(-1) Ca2+ significantly enhanced the accumulation of total alkaloid and guanosine in P. ternata tubers. At Lower concentrations of Ca2+, the content of total free organic acid was higher in the tuber.

CONCLUSIONWith the treatment of Ca2+ the capacity of heat resistance was improved in P. ternata plants, the rate of lodge P. ternata was postponed, growing period was extended and corresponding production has increased by spraying exogenous Ca2+.

Calcium Compounds ; pharmacology ; Hot Temperature ; Pinellia ; chemistry ; drug effects ; Protective Agents ; pharmacology ; Stress, Physiological

OBJECTIVETo explore the inhibitory effects of Niaoluqing, an oral liquid of traditional Chinese medicine, on the growth of urogenital chlamydia trachomatis (Ct).

METHODSNiaoluqing's applying concentration was 1 g/ml and 10 serologically untyped strains of Ct from the STD clinic were used. And the inhibitory effects of Niaoluqing on Ct was evaluated by McCoy cell microculture technique in vitro.

RESULTSNiaoluqing had inhibitory activity for urogenital Ct, and was capable of reducing inclusion numbers notably in the concentrations of 50 to 200 mg/ml. The number and volume of Ct inclusions reduced gradually and disappeared finally with the rising of the medicinal concentration.

CONCLUSIONThe traditional Chinese medicine Niaoluqing has inhibitory effects on the growth of urogenital Ct.

Chlamydia trachomatis ; drug effects ; isolation & purification ; Dose-Response Relationship, Drug ; Drugs, Chinese Herbal ; pharmacology ; Female ; Humans ; Male ; Microbial Sensitivity Tests ; Urogenital System ; microbiology

OBJECTIVETo identify the mutations of the tyrosinase gene (TYR) and P gene in patients with oculocutaneous albinism (OCA).

METHODSPolymerase chain reaction (PCR) and denaturing high performance liquid chromatography (DHPLC) were applied to detect the mutations in all exons of TYR gene and P gene. Then DNA sequencing and restriction endonuclease analysis were used to confirm the mutations detected by DHPLC. Novel mutations were screened in 100 unrelated persons with normal phenotypes to exclude the possibility of polymorphism.

RESULTSTwo mutations were detected in the P gene of the three patients and none in TYR gene. Heterozygous mutation of T450M in exon 13 of the P gene was detected in patient 1. Patient 2 had a heterozygous mutation of T450M in exon 13 and a heterozygous mutation of G775R in exon 23 of the P gene. Patient 3 had a heterozygous mutation of G775R as well. Restriction endonuclease analysis of the P gene exon 13 showed that the Oli I site had partly disappeared resulting from the heterozygous mutation T450M in patient 1 and patient 2, but not in 100 unrelated individuals. The heterozygous mutation T450M is a novel mutation.

CONCLUSIONGene diagnosis of OCA can be carried out effectively by combining PCR, DHPLC, DNA sequencing and restriction endonuclease analysis.

Albinism, Oculocutaneous ; genetics ; Base Sequence ; Catechol Oxidase ; genetics ; Child, Preschool ; DNA Mutational Analysis ; Exons ; genetics ; Female ; Hermanski-Pudlak Syndrome ; genetics ; Humans ; Monophenol Monooxygenase ; genetics ; Mutation ; Young Adult

Objective To investigate the influences of verapanal preconditioning on cardiac function in vitro and intracellular free Ca~(2+) and L-type calcium current (I_(Ca-L)) in rat cardiomyocytes post ischemiareperfusion (I/R) injury. Methods The isolated rat hearts in control group (37 ℃ Tyrode solution perfusion for 30 min, n =6), I/R group ( no flow for 30 min followed 30 min reperfusion with 37 ℃ Tyrode solution, n =7) and verapamil preconditioning group [37 ℃ Tyrode solution perfusion for 10 min, adding verapamil (20 μmol/L ) to Tyrade solution and perfusion for another 30 nan, followed then by 30 min no flow and 30 min reperfusion, n=7 ] using Langendorff perfusion system. The fluorescence intensity of intracellular Ca~(2+) was detected with Fluo-3/AM loading by the laser scanning confocal microscope. The I_(Ca-L) was recorded via whole-cell patch clamp technique in enzymatically dissociated single rat ventricular myocytes. Results As expected, arrhythmias and cardiac dysfunction were shown post I/R injury. The fluorescence intensities of intracellular free Ca~(2+) in cardiomyocytes were significantly increased compared with control group (P <0. 01 ). By voltage clamp protocol, peak current densities of I_(Ca-L) was significantly reduced and I-V curve significantly elevated. Post I/R injury compared with control group (P < 0. 01 )which could be reversed by Verapamil preconditioning. Verapamil preconditioning also significantly improved diastolic and systolic functions, and reduced the incidence of arrhythmias. Conclusions Myocardial I/R injury might significantly impair heart functions and induce arrhythmias via cellular Ca~(2+) overload.Verapamil preconditioning could prevent heart I/R injury and reduce arrhythmias by decreasing influx of I_(Ca-L), thereby stabilizing cardiomyocytes in myocardial stunning and avoiding occurrence of Ca~(2+) -induced Ca~(2+) release during I/R injury.

Chromosomes, Human, Pair 22 ; Chromosomes, Human, Pair 8 ; Humans ; Myeloproliferative Disorders ; Receptor, Fibroblast Growth Factor, Type 1 ; Translocation, Genetic

Objective To analyze the general characteristics of suicide in patients with mental illness,to discuss the characteristics of suicide death,and to provide reference for the social intervention of suicide in the early stages.Methods We collected the suicide cases of 438 patients with mental illness in Shanghai Pudong New Area from 2007 to 2016,and analyzed their sex,age,way of death and cause of death.Results In these 438 cases of suicide,women were significantly more than men.The age mainly distributed between 30 and 70 years old,and the highest of incidence rate occurred between 60 and 70 years old.Among these patients,the youngest one was 11 years old,and the oldest one was 93 years old.Depression (48.2％) was the leading cause for suicide,followed by schizophrenia (16.7％),paranoia (1.6％) and anxiety (0.9％).Jumping from height (43.6％) and hanging (31.5％) were the most common ways of suicide.There was pre-signal (suicide notes,expression of suicidal and suicidal history) in 47 cases.Conclusions Mental disorders,especially depression,has become an indispensable fator in the suicide people.We should pay enough attention to early intervention and treatment in order to avoid tragedy again.

OBJECTIVETo investigate whether HV and Ot can coexist in their host (Leptotrombidium scutellare).

METHODSCollecting the separate Leptotrombidium scutellare and the ones from mice in epidemic area. The cells of mites at larva, nymph, and adult stages were cultured and made into smear. In situ RT-PCR and PCR were used to detect and locate HV RNA and Ot DNA in the primary cultured cells.

RESULTSPositive signals of HV RNA and Ot DNA distributed mostly in epithelial cells of digestive system and ovary cells of larva and nymph. The positive rate increased by the generation of passages.

CONCLUSIONCoinfection of HV and Ot did exist in wild Leptotrombidium scutellare.

Animals ; Cells, Cultured ; DNA, Bacterial ; analysis ; Female ; Hantavirus ; isolation & purification ; Mice ; Mites ; microbiology ; virology ; Orientia tsutsugamushi ; isolation & purification ; RNA, Viral ; analysis ; Reverse Transcriptase Polymerase Chain Reaction

Objective To present a new mini-invasive surgery for compressive optic neuropathy (CON) in Graves' ophthalmopathy (GO) by adequately decompressing the orbital apex and correcting proptosis,and to analyze its results.Methods A retrospective chart was reviewed in 29 patients receiving orbital decompression for the treatment of CON secondary to GO from October 2006 to May 2011.All patients diagnosed CON were in stable and inactive phase of GO at least for 6 months.All patients received endoscopic transethmoid medial orbital wall decompression to reduce the compression on the orbital apex.In the meanwhile,an endoscopic transethmoid intraconal fat-removal orbital decompression was performed to remove parts of intraconal fat with a special aspiration/cutting instrument to further reduce the proptosis.All patients were followed up periodically.Results of improvement of visual acuity ( VA),color vision,and amount of proptosis reduction and incidence of induced diplopia 9 months after surgery was recorded for analysis its feasibility.Results Forty-five orbits of 29 patients were included in the study.At the 9 months review,44 of 45 eyes (97.8％) improved their VA from -0.65 ±0.30 (x ±s) preoperatively to -0.24 ± 0.22,with a mean improvement of 0.55 ± 0.17 ( t =- 13.01 2,P ＜ 0.001 ),23 of 29 eyes ( 79.3％ ) had improved color vision ( P ＜ 0.001 ),and the mean reduction in proptosis was ( 7.07 ± 1.59 ) mm ( range 4- 11 mm).Postoperative symmetry to within 2 mm were achieved in all patients.Except 1 patient complaining of deterioration in diplopia following surgery,no patients presented new on-set diplopia postoperatively.Conclusion The endoscopic transethmoid medial orbital wall decompression combined with the endoscopic transethmoid intraconal fat-removal orbital decompression is an effective treatment with minimal morbidity for both visional recovery and improvement of proptosis for CON in GO.