Objective: To analyze the cost of laparoscopy and laparotomy in the treatment of tubal pregnancy.Methods: The selected patients of tubal pregnancy were operated on in our hospital from Jan. 2000 to Jun. 2002. Eighty received laparoscopy and 13 received laparotomy.The off work day, hospital charge, cost of no work and total cost were analyzed and compared.Results: The mean hospital stay and off work days were significantly shorter in the laparoscopy group than in the laparotomy group( P

Objective: To introduce an improved direct method for first puncture and to study the safety of first puncture in laparoscopic surgery.Methods: Four kinds of techniques were used to perform the first puncture on 4 102 patients.Group A: trocar puncture were performed after pneumoperitoneum in 2 492 cases.Group B: trocar puncture was directly through the incision of umbilical skin in 798 patients; Group C: trocar puncture was directly through the incision of umbilic skin,subcutancous fat and fascia in 387 patients.Group D: opened umbilical hole and inserted the trocar shell without puncture in 425 cases.it was a success if entered the abdominal cavity at the first time,it was unsafe if punctured twice and a failure if the needle hadn't entered the abdominal cavity for 3 times or went into the subcutaneous vessels or viscera.Results: There were no vessels and viscera injury during punctures in our study.The puncture results were related to the different methods.Compared with group A, the success rate of group C and D were significantly improved( P 0.05).Success rates of group C and D were significantly higher than that of group A.The success rate was higher in group D than in group C( P

Objective To investigate the effects of 0.9 g/dl NaCl diluting instrument method to solve the ethylenediamine tet-raacetic acid dipotassium (EDTA)anticoagulant dependency pseudo reduce platelet syndrome (PTCP)feasibility,provides solutions to clinical laboratory PTCP more effective method.Methods From August to October of 2014 in their laboratory for PTCP cases in all 3 cases,2 ml venous blood in EDTA and citron acid sodium anticoagulation in-line blending,in the im-mediate,10,30,40 and 60 min computer detection.Collected of peripheral blood in blood thinners,respectively,0.9 g/dl NaCl solution blending,in the immediate,10,30,40 and 60 min computer detection,and compared with the manual method of ammonium oxalate.Results EDTA,citron acid sodium,blood thinners and 0.9 g/dl NaCl diluting instrument immediately detected PTCP blood PLT result compared with ammonium oxalate method,there were no statistically significant difference (t=0.943~1.537,P >0.05),10 min~60 min anticoagulant blood PLT results significantly decreased,compared with am-monium oxalate method difference had statistical significance (t = 12.413 ~ 12.413,P <0.01 ~0.001).Citron acid sodium PLT began to decline after 30 min,compared with ammonium oxalate method,the difference was statistically significant (t=4.915~4.915,P <0.05~0.01).Blood dilution method in 30~40 min PLT test results began to decline,but not obvious, there was no statistically significant difference with the method of ammonium oxalate (t=1.315~1.715,P >0.05),40~60 min PLT test results appear significantly decreased,and the method of ammonium oxalate difference was statistically signifi-cant (t=3.175~3.175,P <0.05);Within 0~60 min 0.9 g/dl NaCl method to detect the PLT differences between the re-sults with the method of ammonium oxalate had no statistical significance (t=0.694~ 1.062,P >0.05).Conclusion ED-TA,citron acid sodium,blood thinners and 0.9 g/dl saline diluting instrument immediately detected PLT PTCP patients were consistent with ammonium oxalate method.Citron acid sodium within 30 minutes and blood dilution method in patientswith PTCP PLT detection could achieve ideal effect,but there were still a small amount of PLT gathered and led to a slight drop in PLT.0.9 g/dl saline diluting instrument method with ammonium oxalate within 0~60 minutes method to detect the PLT result had no difference.

Objective To survey the clinical effect of combination therapy of Ninggan-Tiaozhong decoction and psychological intervention for chronic atrophic gastritis (CAG).Methods 80 cases CAG were randomly divided into a treatment group (treated with Ninggan-Tiaozhong decoction and psychological intervention) and a control group (treated with Ninggan-Tiaozhong decoction),with 40 cases in each group and performed a therapy for 12 weeks.The clinical efficacy and the change of Symptom Checklist (SCL-90)and Self-Rating Anxiety Scale(SAS) before and after treatment were evaluated.Results After 12 weeks of treatment,the total effective rate was 90.0％ in the treatment group,and 74.4％ in the control group.The treatment group was better than the control group(x2=4.255,P＜0.05).After psychological intervention,SCL-90 and SAS score (39.5±4.3,1.3± 0.6)was significantly reduced in the treatment group than the control group (51.4±3.2,1.5±0.3),(P＜0.05).Conclusion The combination therapy of Ninggan-Tiaozhong decoction and psychological intervention used in CAG is effective to relieve symptoms and to improve life quality of patients.

Objective To understand the different types of chronic hepatitis group in shenzhen area E viral hepatitis (hepatitis E infection status and provide scientific basis for the prevention and treatment of hepatitis E.Methods Randomly collected from July 2013 to June 2015 in shenahen Longhua New District People’s Hospital without hepatitis normal physical exami-nation and treatment group 1746 cases as control group,chronic hepatitis B viral hepatitis (hepatitis B)the crowd of 1 320 cases of hepatitis B,chronic viral hepatitis C (HCV)population group,615 cases of hepatitis C,respectively,the application method of enzyme-linked immunosorbent assay (ELISA)to detect serum hepatitis E antibody (anti HEV IgG)-IgG,ana-lyzed of different types of hepatitis a crowd hepatitise infection status,and compared the hepatitise infection rate if there was a difference between the different groups.Results 1 746 cases of control group in serum anti HEV-IgG positive rate was 3.49% (61/1 746),4.22% of men and women in 2.68%.1 320 cases of hepatitis B group was 10.9%,12.29% of men and women in 8.23%.615 cases of C group was 10.2%,12.35% of men and women in 7.64%.Hepatitis B and C group of HEV-IgG positive rate compared with control group difference was statistically significant (χ2 =9.163~9.405,P <0.05), and hepatitis B and hepatitis C group there was no statistically significant difference between positive HEV-resistant IgG (χ2=0.614,P >0.614),and hepatitis E infection rate than women,men was statistically significant difference (χ2 =2.873~4.025,P <0.05).Conclusion Chronic hepatitis B and C viral hepatitis crowd anti HEV-IgG positive rate was higher than normal people,no hepatitis hepatitis E infection rate among men than women.Therefore,strengthens to the chronic hepatitis B and C viral hepatitis a crowd of early discovery,early diagnosis and early treatment,to reduce hepatitis E infection rate has an important significance.

Objective To investigate the expression of transforming growth factor-?(TGF-?) in the villus and deciduas of different outcomes after administration with mifepristone and misoprostol in the termination of early pregnancy. Methods From May 2004 to March 2005,50 women who were comfirmed early pregnancy and requested termination of pregnancy in the outpatient department were recruited.Patients with medical abortion were divided into two groups according to the final outcomes: group of complete abortion(n=15) and group of incomplete abortion((n=)15).Another 20 patients with surgical abortion were served as control.The villus and deciduas were collected at the third day of medical abortion,whereas the same was done at the day of surgical abortion.The expression of TGF-? in villus and deciduas of the three groups were measured by immunohistochemical method. Results The age,gestational age,diameter of gestational sac and levels of serum E_(2),P and ?-HCG before abortion were similar in these groups.Compared with the other two groups,the expressions of TGF-? in the villus and deciduas in the group of complete abortion were significantly decreased(P

Animals ; Caudate Nucleus ; drug effects ; physiology ; Dopamine Antagonists ; pharmacology ; Rats ; Rats, Wistar ; Substantia Nigra ; drug effects ; physiology

Objective:To evaluate the performance of Sysmex XN-9000 automatic blood analyzer (hereinafter referred to as XN-9000) in whole blood with the low platelet (PLT). Methods:A total of 48 whole blood samples with platelet count less than 70×109/L were randomly collected from XN-9000 correlation analysis to evaluate XN-9000 the accuracy and precision of detection in low whole blood PLT and carry pollution rate, use XN-9000 and microscope count, respectively.Results: XN-9000 detection in low whole blood PLT between batch and batch of CV% 1.13% and 1.29%, respectively; XN-9000 with microscope PLT count results have high correlation, The regression equation forY=0.917X+3.217,correlation coefficientr=0.964; XN-9000 detection in low whole blood PLT carry pollution at a rate of 0.12%. Detection accuracy of PLT Bias, %(-1.25%~1.8%); Linear range(0~1206)×109/L; Clinical reportable range (0~6030)×109/L.Conclusion: The detection of XN-9000 blood analyzer is in low whole blood PLT has high precision and accuracy, carry pollution rate is extremely low, the result has the reliability, can be directly applied to clinical.

Objective To understand the shenzhen longhua new district and the light district three third district hospital pseudomonas aeruginosa infection the clinical distribution and drug resistance,for clinical provides the basis for scientific and medical treatment.Methods Collected 3 176 clinical specimens in three district hospital from June 2013 to November 2014 and they were done bacteria identification with VITEK-32 bacteria identification instrument of French biomerieux.For pseudomonas aeruginosa specimens using the K-B method and trace the broth dilution method (MIC)to do drug sensitive test,and the inspection results were statistically processed.Results 3 176 specimens pseudomonas aeruginosa isolated total separation rate was 51.16% (1 625/3 176),including respiratory sputum specimens was 52.8% (858/1 625),followed by bronchoalveolar lavage and pus,were 20.1% (327/1 625)and 16.7% (271/1 625).Ward,neurosurgery and thoracic sur-geons are mainly distributed in the ICU,were 41.6% (676/1 625),15.9% (259/1 625)and 19.1% (310/1 625).Carbon penicillium,resistance to carbon alkene sensitive penicillium alkene and extensive drug resistance rate of pseudomonas aerug-inosa isolated were 67.1% (1 090/1 625),31.6% (514/1 625)and 1.29% (21/1 625).Resistance to carbon penicillium al-kene the drug resistance of pseudomonas aeruginosa from penicillium carbon alkene sensitive serious,in addition to the poly-myxin B resistance to both comparative difference was statistically significant (χ2 = 12.617~ 12.617,P <0.05~0.001),2 cases of resistance to carbon penicillium alkene pseudomonas aeruginosa to polymyxin B resistance,in addition to amikacin, gentamycin,tobramycin has high sensitivity,the rest of the 11 kinds of antimicrobial drug resistance to all>60%.Conclusion Clinical pseudomonas aeruginosa had a high separation rate,mainly comes from the respiratory tract and the distribution of the ICU ward.Penicillium carbon alkene resistant pseudomonas aeruginosa than carbon penicillium sensitive resistance was serious,should pay close attention to carbon blue mould resistant pseudomonas aeruginosa resistance development,take effective measures of preventing transmission and infection of scientific use of antimicrobials,put an end to resistance to car-bon penicillium alkene and the spread of drug resistance pseudomonas aeruginosa .

Objective To investigate and dispose an outbreak of seasonal influenza in hospital,so as to provide reference for the prevention and control of influenza outbreak in hospital.Methods Eight cases of influenza-like in-fection occurred in the department of neurosurgery at a hospital between July 29 and August 7,2014,epidemiologi-cal investigation was conducted,throat swabs of infected persons were collected for laboratory detection.Results Of 8 infected persons,6 were health care workers (HCWs)in department of neurosurgery,1 was a family member of HCW,and 1 was a patient,the major symptoms of the infected persons were low-grade fever,sore throat,and ma-laise,there were 67 patients and HCWs in this department,the attack rate of influenza was 11.94%,there was no similar infection in other departments of the hospital during the same period.The throat swabs from 6 infected HCWs were positive in influenza virus nucleic acid detection.Office for healthcare-associated infection (HAI)man-agement participated the investigation,after active isolation and antiviral treatment,the outbreak was effectively controlled.Conclusion This HAI outbreak is a seasonal influenza H3 outbreak,ventilation and environmental dis-infection in wards should be strengthened when central air conditioning is running,anti-influenza vaccination among HCWs should be performed during the epidemic season of influenza,and surveillance should be strengthened to pre-vent influenza outbreak in hospital.