OBJECTIVE: To establish the model of skin scald in mice for the study of skin thermal injuries. METHODS: After anaesthetization mice were scalded in a 1 cm-diameter circle area on the central dorsum by boiling water at contact times: 10s or 25s. The mice were sacrificed at 1, 3, 5, 7 and 10 days after scald. The skin samples were collected and analyzed by gross and histopathological examinations. RESULTS: Deep II degree thermal injury involving full-thickness skin was observed in the 10s scald group. III degree thermal injury involving full-thickness skin and the dorsal skeletal muscle was observed in the 25 s scald group. CONCLUSION A mouse skin scald model is established which is stable and can be used on the skin thermal injury in future research.
Animals ; Burns/pathology* ; Disease Models, Animal ; Epithelium/pathology* ; Female ; Hot Temperature/adverse effects* ; Male ; Mice ; Random Allocation ; Reproducibility of Results ; Severity of Illness Index ; Skin/pathology* ; Time Factors