Objective To analyze the mutations in nucleotide sequences of transfusion transmitted virus(TTV) in neonatal infection.Methods Neonatal serum TTV-DNA was detected by a nested PCR technique.Fifteen Chinese neonates with positive TTV-DNA were diagnosed as TTV infection.ORF1 sequences of TTV-DNA from these neonates were determined.Results Homology of Chinese TTV(C01-C15) and Japanese TTV(N22)isolated ranged from 87.1%-97.7% at nucleotide level,but there were point mutations in Chinese TTV,such as GG→TT in locus 112 and 113,TTATC→CCTAT in locus 236-240.Conclusions Chinese and Japanese TTV isolated had the same genotype.Some gene mutations may increase the TTV pathogen,and result in neonatal hepatitis syndrome or hyperbilirubinemia.

Objective To investigate the medicated side warm nest pot combined with letrozole in the treatment of multiple bursal ovarian syndrome (PCOS) superiority of the clinical treatment of kidney deficiency syndrome in ovulation dysfunction infertility,provide the basis for clinical treatment. Methods 78 cases of infertile patients(randomly selected from January 2014 to June 2015 hospitalized in Hainan Medical University Hospital) due to kidney deficiency syndrome in patientswith ovulatory dysfunction resulting from PCOS. 39 cases in the control group received oral letrozole only. 39 cases in experimental group took oral warm nest pot combined with letrozole,continuous treatment for four menstruation cycle. Adverse reactions,ovulation rate,pregnancy rate and safety index of the two groups before and after treatment of menstruation were observed and recorded. Results The effective rate of the control group was 74.36%,and the effective rate of the experimental group was 87.18%. The difference was statistically significant(P < 0.05). The ovulation rate of the control group was 47.71%,which was lower than that of the experimental group (79.07%),and the difference was statistically significant (P < 0.05). The pregnancy rate in the control group was 12.82%,while that in the experimental group was 33.33%,and the difference was statistically significant (P < 0.05). Patient′s irregular menstruation improved in the two groups , while the experimental group did better than the control group. Conclusion Effect of warm nest pot combined with letrozole on kidney deficiency syndrome of ovulation dysfunction in PCOS is remarkable ,with little side effects.

A questionnaire was used to survey the perspective of the 22 mentors,24 students, 27 graduate students and 27 public health units on the competence of MPH student. The result showed that the practice skill,analyzing skill,program plan and practice skill,and emergency reaction abil-ity got more attention. This suggested that to the training of MPH,the training objective should focus on the ability to resolve problems; the curriculum should focus on the characters of public health and on the practice training.

Objective To investigate the effect of video-based teaching in the training of nursing operation for newly-contracted nurses. Methods One hundred and twenty one nurses newly recruited in September 2011 to September 2012 were set as the control group, another 128 in October 2012 to October 2013 were set as the experiment group. The former were trained and assessed with traditional training method and the latter were trained for 1 year in video-based teaching methodology. After training, both groups were examined about their operation skills and meanwhile a survey on video-based teaching was conducted. Result After training, the results in operating skills evaluation in the experiment group were significantly better those of the control group (Z=2.82, P<0.05). Conclusion Compared with traditional training method, the video-based teaching can raise the level of nursing operation skills and significantly improve the quality of nursing, thus worthy of popularization and application.

Objective To evaluate the training quality of anaesthesiology residents in different hospitals. Methods The scores of 125 residents,attending Shanghai resident clinic skill examination from 2005 to 2007,were analyzed. ResultsThe scores of examinees from municipal hospitals were higher than those from district hospitals,municipal specialized hospitals and traditional Chinese medicine hospitals.The scores of practice part were higher than those of theory part in all groups. Conclusion The training quality is related to the comprehensive strength of the hospital in which examinees work,but the training of theory knowledge still need to be strengthened.

Animals ; Blood Urea Nitrogen ; Cadmium Poisoning ; blood ; metabolism ; pathology ; urine ; Disease Models, Animal ; Kidney ; drug effects ; pathology ; Male ; Rats ; Rats, Sprague-Dawley

Adult ; Aged ; Cerebral Infarction ; etiology ; China ; epidemiology ; Coronary Disease ; diagnosis ; etiology ; Diagnosis, Differential ; Female ; Humans ; Hypertension ; complications ; diagnosis ; epidemiology ; Logistic Models ; Male ; Medicine, Chinese Traditional ; Middle Aged ; Risk Factors ; Yin Deficiency ; diagnosis

Glucose was transported by the large number of hexose transporters in yeast cells. There were 18 hexose transporter genes had been identified in Saccharomyces cerevisiae. However,as an excellent expression system,there was no information of these genes had been reported in Pichia pastoris. Based on high homologous recombination efficiency in yeast,we chose G418 resistance for screening,200 bp were cloned from the up and down sequences of HXT1 ORF respectively,then ligated to the 5′ and 3′ end of G418 resis-tance gene for recombination. After electroporation of GS115 spheroplast and screened through different G418 concentration plates,finally we obtained one HXT1 gene deletion mutant named GS115?HXT1. The growth rate and glucose consumption of this mutant were both lower than the wide type.

Aristolochic Acids ; adverse effects ; Child ; Drugs, Chinese Herbal ; adverse effects ; Female ; Humans ; Male ; Nephrosis ; chemically induced