Objective:To study the impact of palatal scar force on the maxilla with unilateral cleft palate by finite element model. Methods:A 3-dimension finite element model of maxilla with cleft palate containing soft tissue was established,the soar force on the maxilla was analyzed by ansys.Results:Cleft area was identified as the center of rotation,cleft side of the maxilla reflected clock-wise rotation and non-cleft side anticlockwise rotation.The maxilla moved from outside to inside in cross section and front to back in the sagittal plane,and the former was greater than the latter.The outside of alveolar process was subjected to stress of mainly drawing force,the inside to stress of mainly compressive force.The deformation of maxilla was mainly in the front-end.Deformation of the alveolar was greater than that of maxilla body.Among deformations of three dimensions,the width was the largest,the length was the smallest,and the height was between both above.Conclusion:Along with the rotation of the maxilla,cleft area becomes gradually smaller.Among the three dimensions,the width is the largest,the length is the smallest and the height is between the both.The af-fect on maxilla is mainly in the front-end.The affect on the alveolar is greater than that on the maxilla body.

Objective:To establish a soft tissue 3-dimension(3D)finite element model of human palate.Methods:Based on the intrinsic finite element model of human maxilla,palatal mucoperiosteum flap with the thickness of 3.06 mm was added on the oral side of the plate model by ANSYS software,material property of the soft tissue was loaded,the soft tissue 3D finite element model of the palate was established.Results:By applying finite element method,the established 3D finite element model could reflect biomechani-cal properties of the soft tissue of the palate on computer.Conclusion:The 3D finite element model of the soft tissue of human palate provides the foundation for further study.

Objective To evaluate the relationship between the clinicopathological factors, prognosis and the expression of p16 and c erbB 2 protein in primary breast cancer. Methods The expression of p16 and c erbB 2 by immunohistochemical method was observed in 50 patients with primary breast cancer and the detection of p16 by polymerase chain reaction(PCR) and the point mutation of p16 by PCR single strand conformational polymorphism(SSCP) were detected in 20 patients with breast cancer. Results Among the cancers, positive expression of p16 protein was found in 17(34.00%) cases, c erbB 2 protein positive expression in 24(48.00%) cases. No homozygous deletion in p16 gene was found. However, exon2 point mutation of p16 gene was found in 1 of 20 breast cancer. The results showed no relationship between p16 expression and clinicopathological factor or prognosis. Positive expressions of c erbB 2 protein were often found in breast cancer with lymph node metastasis(P=0.0237) with a poor 5 year survivalrate(P=0.0169). There was no consistent relationship between the expression of p16 and c erbB 2 protein. Neither p16 nor c erbB 2 protein expression could be as an independent prognostic factor. Conclusions The patients with breast cancer of positive expression of c erbB 2 protein has a high lymph node metastasis rate and a poor survival rate. The point mutation rate of p16 gene is lower in primary breast cancer, and it can be a molecular events in advanced primary breast cancer.

ObjectiveTo study the intelligence quotient(IQ) profile of the children with idiopathic generalized tonic-clonic seizure (GTCS) and the factors influencing IQ of them.MethodsAll 28 children with GTCS were selected who were aged 9 ～ 16 years in 20 GTCS families in a mountain area of the south of Anhui Province,all available healthy siblings of the children included in the epilepsy group,did not have epilepsy,and were nearest in age to the children with GTCS ( control group 1 ),and thirty children aged 9 ～ 16 years old who had lived in the same village ( control group 2) entered into our study.The IQ was studied of the three groups of children and the factors influencing IQ of children with GTCS at the same time.The data were input SPSS12.0 and analyzed.ResultsThe IQ of children with GTCS(85.64 ±20.57)was lower than that control group 1( 103.39 ± 11.17)and the control group 2 ( 106.17 ± 11.67).The difference between children with GTCS and the two control groups were significant for almost all the subtest quotients except completion of drawing and picture arrangement.No significant differences were found between the control group 1 and the control group 2 on the IQ and the subtest quotients.IQ scores of children with GTCS showed significant linear correlation with father's education( r=0.453,P＜0.01 ),age at onset of epilepsy( r=0.506,P＜0.01 ),duration of seizure disorder( r=-0.533,P＜0.0l ),status epilepticus( r=-0.732,P＜0.01),total number of seizures( r=-0.761,P＜0.01) and seizure frequency ( r=-0.708,P ＜ 0.01 ).ConclusionThe IQ scores of the children with idiopathic GTCS are lower significantly than general children population.Epilepsy-related variables affecting IQ scores of the children with idiopathic GTCS are duration of seizure disorder,status epilepticus,age at onset of epilepsy,total number of seizures,seizure frequency.

Ectodermal Dysplasia 1, Anhidrotic ; diagnosis ; genetics ; Ectodysplasins ; genetics ; Heterozygote ; Humans ; Infant, Newborn ; Male ; Mutation, Missense

Adrenal Cortex Hormones ; therapeutic use ; Anemia, Hemolytic, Autoimmune ; diagnosis ; drug therapy ; Female ; Humans ; Infant ; Treatment Outcome

This study is to investigate the sedative and hypnotic effects of a novel compound H1208. The sedative activity of H1208 was investigated by recording the spontaneous locomotor activity of mice. The hypnotic property was evaluated by the latency and duration of sleep (loss of righting reflex) in mice and the effect of hypnotics on sleep pattern of electroencephalogram were studied in conscious, freely moving mice with chronically implanted electrodes. The brain monoamine neurotransmitters levels in mice were measured by high performance liquid chromatography-electrochemical detection. The spontaneous locomotor activity was decreased by 56.7% and 80.2% in H1208 (5 and 25 mg x kg(-1), ip) treated mice, respectively. The loss of righting reflex was directly induced in mice after H1208 (60 mg x kg(-1), ip) administration. The non-rapid eye movement sleep increased significantly by 131% and 259%, respectively, within 3 hours after H1208 (30 and 60 mg x kg(-1), ip) administration. However, the rapid eye movement sleep decreased significantly. The contents of DA in the striatum and cortex and 5-HT in the cortex decreased significantly. These results demonstrated that H1208 has potent sedative and hypnotic effects, which may be closely related to the decreased contents of DA and 5-HT in mouse brain.
Animals ; Brain ; drug effects ; physiology ; Dopamine ; metabolism ; Electroencephalography ; Hypnotics and Sedatives ; pharmacology ; Mice ; Motor Activity ; drug effects ; Serotonin ; metabolism ; Sleep ; drug effects

Data mining technology has become a powerful tool in traditional Chinese medicine (TCM) research. In this paper, based on the principle and basic requirements of data mining, the mining methods and procedures were described. And then the application of data mining technology in Chinese medicine pair research was classified and summarized, such as the compatibility characters, characteristic pairs, dosage-effect relationship and property compatibility, which provide the direction and data base for modern research of Chinese medicine pair.
Cluster Analysis ; Data Mining ; methods ; Drug Interactions ; Drug Prescriptions ; Medicine, Chinese Traditional ; methods

OBJECTIVETo investigate the effects of drug-contained sera (DCS) of Naoluo Xintong (NLXT) and Zuogui Pill (ZGP) on the proliferation and differentiation of in vitro cultured embryonic neural stem cells (NSCs) in rats.

METHODSRat's embryonic NSCs were cultured in medium supplemented with 10% DCS with NLXT and ZGP separately, the effects of DCS in enhancing proliferation and inducing differentiation were observed and compared by phase contrast microscopy and immunofluorescence staining.

RESULTSMajority of NSCs were induced to neurons, astrocytes or oligodendrocytes in medium supplemented with either DCS-NLXT or DCS-ZGP, with the growth of them promoted to some extent. However, DCS-NLXT induced the differentiation rather slowly but with the differentiated neurons more resemble to the mature neuron in morphology, while DCS-ZGP promoted the stem cell growth more effectively.

CONCLUSIONBoth NLXT and ZGP could promote the differentiation and growth of in vitro cultured NSCs, but with exiguous difference. It is feasible to induce the proliferation and differentiation of NSCs by way of using Chinese medicine drug-therapy for supplementing qi and activating blood circulation as well as that for tonifying Shen to generate marrow.

Animals ; Cell Differentiation ; drug effects ; Cell Proliferation ; drug effects ; Cells, Cultured ; Drugs, Chinese Herbal ; pharmacology ; Female ; Male ; Neural Stem Cells ; drug effects ; Pregnancy ; Rats ; Rats, Sprague-Dawley ; Rats, Wistar ; Serum ; chemistry

OBJECTIVE: To study a surgical and repairing method for defects after the resection of basal cell carcinoma of external nose. METHOD: There are 26 cases of basal cell carcinoma that tumors have been resected completely after operation. For defect repairing in those 26 cases, 2 cases adopt direct suture method; 2 cases use skin graft repairing methods; 18 cases employ naselabial skin flap repairing method; 4 cases choose forehead pedicle skin flap repairing methods. RESULT: the wound of all the 26 cases was primary healed. Additionally, skin flap and skin graft were all survived. Follow-up studied in patients 1 to 3 years after the surgery showed that the local scar was not obvious and no tumor recurred or transferred. CONCLUSION Different surgical and repairing methods are performed to obtain a satisfactory results based on the area of defect and its location in nose. Naselabial skin flap is especially an ideal method to repair defects.
Carcinoma, Basal Cell ; surgery ; Cicatrix ; Forehead ; Humans ; Neoplasm Recurrence, Local ; Nose ; Nose Neoplasms ; surgery ; Reconstructive Surgical Procedures ; Skin ; Skin Neoplasms ; surgery ; Skin Transplantation ; Surgical Flaps ; Wound Healing