Antibodies, Monoclonal ; analysis ; Biopsy, Needle ; Breast Neoplasms ; chemistry ; pathology ; Female ; Humans ; In Situ Hybridization ; methods ; In Situ Hybridization, Fluorescence ; Practice Guidelines as Topic ; Quality Control ; Receptor, ErbB-2 ; analysis ; immunology ; Receptors, Estrogen ; analysis ; Receptors, Progesterone ; analysis ; Societies, Medical ; Specimen Handling ; United States

Objective To observe the changes of the quality of life in type 2 diabetes accompanying subclinical atherosclerosis and explore the effect of diabetic macrovascular complications in the quality of life.Methods One hundred and thiry-six type 2 diabetes were measured carotid intima media thickness (IMT) and then divided into AS group(n =51) and CON group(n =85).Two groups were examined with Special of Quality of Life for Diabetes Mellitus (DSQL).Plasma glucose,plasma insulin,glycosylated hemoglobin(HbA1c),high sensitivity C-Reactive Protein (hs-CRP) as well as insulin resistance index (HOMA-IR) and body mass index (BMI) were observed.Results The scores of DSQL and all domains had obvious difference between AS group and control group(P ＜0.05 orP ＜0.01) ;Relative to the control group the AS group was significantly increased BMI,HbA1c levels,hsCRP levels.The DSQL was associated with IMT,BMI,HbA1c,hsCRP,HOMA-IR.Conclusion The diabetic macrovascular compliations might result in impaired quality of life,which is associated with hyperglycemia,insulin resistance,inflammation,and central obesity.Psychotherapy and health education are very important for the improvement the DSQL in type 2 diabetic accompanying subclinical atherosclerosis.

Adenoma ; metabolism ; pathology ; surgery ; Aged ; Diagnosis, Differential ; Endodermal Sinus Tumor ; pathology ; Female ; Humans ; Immunohistochemistry ; Neprilysin ; metabolism ; Ovarian Neoplasms ; metabolism ; pathology ; surgery ; Sex Cord-Gonadal Stromal Tumors ; pathology ; Stromal Cells ; metabolism ; pathology ; Vimentin ; metabolism

Objective To investigate the expression of miR-16 in spinal cord and the effects of miR-16 mimics on the pain behavior and the expression of its target gene BDNF during the development and maintenance of bone cancer pain.Methods The bone cancer pain model rats were developed by intra-tibia inoculation of Walker 256 mammary gland cells.Before inoculation and 3,5,7,10,14 d after inoculation,the samples of spinal cord L4～6 lumbar enlargement were collected to detect the expression of miR-16 using real-time PCR.Mice in group BM and group BN were intrathcal injected of miR-16 mimics and its negative control products on 10 d,11 d,12 d after inoculation.Paw withdrawal mechanical threshold (PWMT) was measured using von Frey hair mechanical stimulation.The expression of BDNF was detected using western-blot on 14 d after behavior tests.Results Compared with the base level and the level in group S,the significant decrease of miR-16 expression was observed at 5 d～14dingroupT (5d (0.91±0.04),7 d (0.77±0.01),10 d (0.73±0.03),14d (0.42±0.08)) (all P＜ 0.05).Compared with SH group,PWMT was significantly decreased in BC and BP groups at 5 d～ 14 d (P＜0.05),and in group BM at 5 d～ 10 d(P＜0.05).Compared with BP group,PWMT was significantly higher in BM group at 10～12 d(P＜0.05).Compared with SH group,the expression of BDNF was significantly increased in BN and BP groups((2.78±0.31),(2.34±0.23)) (all P＜0.01).Compared with BP group,the expression of BDNF was significantly decreased in BM group (1.42±0.16) (P＜0.01).Conclusion miR-16 is downregulated in spinal cord of bone cancer pain rats,while intrathcal injection of miR-16 mimics can attenuate the pain behaviors in a rat model of bone cancer pain and decrease the expression of BDNF.miR-16 may modulate bone cancer pain through BDNF.

Objective To evaluate the role of spinal IKK2/NF-κB pathway in the maintenance of bone cancer pain (BCP) in rats.Methods Twenty-eight unmated adult female Sprague-Dawley rats,weighing 160-200 g,were randomly divided into 4 groups (n =7 each) using a random number table:sham operation group (group S),BCP group (group BP),BCP + normal saline group (group BN),and BCP + BMS345541 group (group BB).BCP was induced by injecting Walker 256 mammary gland cancer cell suspension 5 μl (4 × 105 cells/ml) into the bone marrow of the right tibia of rats anesthetized with chloral hydrate in BP,BN and BB groups,while the equal volume of normal saline was injected in group S.On 10-12 days after operation,selective IKK2 inhibitor BMS345541 (50 μg/10 μl) was intrathecally injected once a day in group BB,and the equal volume of normal saline (10μl) was given once a day in group BN.The mechanical paw withdrawal threshold (MWT) was measured before intra-tibia injection (T0),on 7 days after intra-tibia injection (T1),at 1 h before drug administration and 1,2,4,12 and 24 h after drug administration on day 10 after operation,and at 4 h after drug administration on day 12 after operation (T2-8).The rats were sacrificed after MWT was measured at Ts and L4-6 segments of the spinal cord were removed for determination of phosphorylated NF-κB (p-NF-κB) expression (using Western blot analysis).Results Compared with group S,MWT was significantly decreased at T1-2,and the expression of p-NF-κB was up-regulated in BP,BN,and BB groups.Compared with group BP,MWT was significantly increased at T4-6,and the expression of p-NF-κB in the spinal cord was down-regulated in group BB.Conclusion Spinal IKK2/NF-κB signaling pathway is involved in the maintenance of bone cancer pain in rats.

Objective To evaluate the effects of Wuqinxi bear play on TCM symptoms and health status in patients with chronic atrophic gastritis of Qi deficiency of spleen and stomach.Methods A total of 60 patients with chronic atrophic gastritis admitted to the Department of Gastroenterology in our hospital were selected.Using random number table method,the patients were divided into the intervention group and the control group with 30 cases in each group.The intervention group adopted bear play for exercise,and the control group was treated with routine treatment and nursing.The scores of TCM symptoms and SF-36,and reports of endoscope were compared between two groups.Results The scores of TCM symptoms in the intervention group were decreased after 2 and 4 weeks' of intervention.The score of each factor in SF-36 was increased and results of endoscope report were improved after 12 weeks' intervention.The differences were statistically significant(P＜0.05).Conclusion Bear play can effectively alleviate symptoms of chronic atrophic gastritis of Qi deficiency of spleen and stomach,and improve patents' quality of life.

OBJECTIVE: To study the gastrointestinal metabolic transformation of polydatin, one of the main bioactive components in Rhizoma Polygoni, thus to figure out the possible biological modification exerted by rat intestinal bacteria in vitro and in vivo, and to determine the possible metabolites. METHODS: The biological samples were analyzed by high performance liquid chromatography (HPLC) and liquid chromatography mass spectrometer (LC-MS/MS) after treatments, respectively. RESULTS: Two main compounds, polydatin and resveratrol, were identified in rat gastrointestinal contents 4 h after the ig administration of polydatin 1500 mg·kg-1. CONCLUSION: This study revealed that polydatin could be metabolized to resveratrol by rat intestinal bacteria through desugarization. Copyright 2012 by the Chinese Pharmaceutical Association.

Objective: To investigate the dynamic accumulations and distributions of eight chemical compounds of Belladonnae Herba in different growing periods and explore the distribution of each ingredient in various parts. Methods: The detection was performed by RP-HPLC with diode array detector (DAD). The mobile phase was acetonitrile-0.05% phosphonic acid in gradient elution. The HPLC method was established for the simultaneous determination of the eight compounds, such as scopolin, chlorogenic acid, quercetin-3-O-galactose (6→1) rhamnose-7-O-glucoside, quercetin-3-O-glucose (6→1) rhamnose-7-O-glucoside, kaempferol-3-O-galactose (6→1) rhamnose-7-O-glucoside, kaempferol-3-O-glucose-(6→1) rhamnose-7-O-glucoside, scopoletin, and rutin, and also for the the comparison on the changes of each ingredient in different growing periods and distribution in various parts. Results: The total amount of each ingredient in the whole plant increased along with its growth. However, each ingredient had different rate of increase, in addition, most compounds would reach to a balance of accumulation in the middle or the last third of June. The analysis results showed scopolin mainly distributed in the roots, while chlorogenic acid had a higher distribution in the leaves and flowers; Scopoletin distributed evenly among all parts in the plant, and the others possessed a higher distribution among the leaves and flowers, however, there was scare distribution in the roots. Conclusion: Each ingredient of Belladonnae Herba has different rate of increase and different distribution in each part, resulting in comparatively large difference of each compound. The experimental data of this research could provide the basis for the implantation, harvest and collection, and quality evaluation of Belladonnae Herba.

Objective: To analyze the contents of eight saccharides in unprocessed and processed Rehmannia glutinosa by HPLC and to investigate the impacts of processing time on saccharides in R. glutinosa. Methods: HPLC conditions were as follows: Prevail Carbohydrate ES column (250 mm × 4.6 mm, 5 μm), flow rate of 0.8 mL/min, ELSD detector, acetonitrile as mobile A and water as mobile B for gradient elution. Results: Fructose, glucose, surcrose, melibiose, raffinose, manninotriose, stachyose, and verbascose were separated with good linear relationships within their corresponding concentration ranges, the r values were within 0.998 4-0.999 7; the average recovery was 97.5%-103.2%. The content analysis at different processing time points showed that surcrose, raffinose, stachyose, and verbascose exhibited decreasing trend during processing, while fructose, glucose, melibiose, and manninotriose exhibited significantly increasing trend. Conclusion: The proportions of saccharides in unprocessed and processed R. glutinosa are significantly different, and the results of this study could provide the data support for revealing the processing mechanism of R. glutinosa.

Objective To observe the lecithin's effect on membrane of African green monkey kidney cells (Vero) exposed to sodium arsenite(NaAsO2). Methods Vero cells cultured in vitro were divided into 4 groups:control group (saline), model group (2.20 mg/L NaAsO2), high eoncentration of lecithin and arsenic group (53.33mg/L lecithin + 2.20 mg/L NaAsO2), low eoncentration of lecithin and arsenic group( 13.32 mg/L lecithin + 2.20 mg/L NaAsO2), 6 bottles of cells in each group, medium was changed every 2 days, cultured for 120 h. Na+ ,K+-ATPase activities of membrane were measured by spectrophotometry, and membrane phospholipids composition including phosphatidylserine (PS), phosphatidylethano-lamine (PE), phosphatidylcholine (PC) and sphingmyelin (SM) were measured by high performance liquid chromatography (HPLC). Results The Na~, K+-ATPase activities of membrane of control group, model group, high concentration of lecithin and arsenic group, low concentration of lecithin and arsenic group were (0.962 ± 0.081) × 106, (0.544 ± 0.037) × 106, (0.647 ± 0.043) x 106, (0.550±Compared with control group, the Na+ ,K+-ATPase activities of other 3 groups were significantly reduced (all P < 0.05). Compared with model group, the Na+ ,K+-ATPase activity in high concentration of lecithin and arsenic group was significantly higher (P < 0.05),but in low concentration of lecithin and arsenic group did not change significantly (P > 0.05). Compared with control group[(0.087 ± 0.003), (0.127 ± 0.053), (0.588 ± 0.105),(0.071 ± 0.029)g/L], PS, PE, PC, SM levels in model group[(0.051 ± 0.018), (0.073 + 0.030), (0.240 ±0.038), (0.047 ± 0.121 )g/L] were significantly lower(all P < 0.05) ;PS, PE, PC in high concentration of lecithin and arsenic group[(0.084 ± 0.011), (0.109 ± 0.363), (0.591 ± 0.476)g/L] did not change significantly(all P > 0.05), but SM[(0.057 ± 0.004)g/L] significantly decreased(P < 0.05) ;PS, PE, SM levels of low concentration of lecithin and arsenic group[(0.058 ± 0.020), (0.086 ± 0.177), (0.048 ± 0.103)g/L] significantly reduced (all P < 0.05), the PC did not change significantly [(0.521±0.098 )g/L, P > 0.05]. Compared with model group,the levels of PS, PE, PC, SM in high concentration of lecithin and arsenic group were significantly higher(all P <0.05);PS, PE, SM levels in low concentration of lecithin and arsenic group did not change significantly(all P > 0.05), and PC was significantly higher(P < 0.05). Conclusions High concentration lecithin has certain protective effect on Vero cell membrane exposured to sodium arsenite.