1.Disinfectant-induced irritating cough in a child.
Chinese Journal of Pediatrics 2011;49(10):800-800
Child
;
Cough
;
chemically induced
;
Disinfectants
;
adverse effects
;
Humans
;
Irritants
;
adverse effects
;
Male
2.In Vitro Cytotoxicity Study of Nickel Ion.
Xiantao WEN ; Wang RUI ; Xuan JIA ; Juli TANG ; Xueying HE
Chinese Journal of Medical Instrumentation 2015;39(3):212-215
The purpose of this study was to investigate the cytotoxicity of the nickel ion and provide with basic data for the biological evaluation of those medical devices containing nickel. Seven cell lines were chosen. They were L929, h9c2(2-1), 293[HEK-293], hFOB1.19, THLE-3, H9 and IM-9 respectively. According to the principle of biological evaluation of medical devices, MTT method was chosen to test the cytotoxicity in different concentrations of nickel ion. For each cell line, the relative growth rate (RGR) was obtained and the cytotoxic grade was classified. Besides, IC50 values were calculated. As a result, it was found that the sensitivity was different among all cell lines. H9 was the most sensitive one, while the L929 was the most tolerant one. The concentration which is not above 1.25 mg/L was safe for all seven cell lines, because the cytotoxicity for all cells exposed in this concentration were not higher than grade 1. According to the criteria for medical devices, the concentrations not above 5 mg/L were safe for L929 cells. This result helps us to roughly assess the cytotoxicity and systematic toxicity caused by nickel contained in medical devices.
Cell Line
;
Equipment and Supplies
;
HEK293 Cells
;
Humans
;
Ions
;
toxicity
;
Nickel
;
toxicity
3.Effect of the early enteral nutrition given through nasojejunal tube in children with acute pancreatitis.
Wen-ji OU ; Si-tang GONG ; Rui-fang PAN
Chinese Journal of Pediatrics 2007;45(8):636-637
Child
;
Child, Preschool
;
Enteral Nutrition
;
adverse effects
;
Female
;
Humans
;
Male
;
Pancreatitis
;
etiology
;
Sodium Chloride
4.Analysis of risk factors in patients with trauma accompanied by multiple organ dysfunction syndrome
Wei ZHU ; Hua-Wen CHEN ; Rui TANG ; Lei WAN ; Qiang ZHONG ; Shu-Sheng LI ;
Chinese Journal of Emergency Medicine 2006;0(12):-
Objective To study the risk factors in patients with trauma accompanied by multiple organ dysfunction syndrome.Method The data of 107 patients with trauma in ICU,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,were retrospectively studied.All patients were divided into 2 groups:MODS group and non-MODS group.The clinical and laboratory,results,ISS score,APACHEⅢscore and GCS score were studied.Results There were no differences on gender,age and mobility of shock between the two groups.There were significant differences on the treatment of shock,ISS score,APACHEⅢscore,CCS score,the levels of blood sugar and platelet between two groups.The Logistic regression analysis showed the main risk factors were treatment of shock,ISS score and APACHEⅢscore.Conclusions The risk factors in patients with trauma accompanied by multiple organ dysfunction syndrome were the treatment of shock, ISS score and APACHEⅢscore.
5.Clinical analysis of progressive epidural hematoma during the operation of brain traumatic injury
Minghao SONG ; Zhixiang LI ; Zhong TANG ; Wenbin MA ; Rui WEN ; Jiangsheng CHENG
Chinese Journal of Primary Medicine and Pharmacy 2012;19(15):2252-2253
Objective To analyze the cause of acute brain swelling,brain encephalocele in brain traumatic injury craniotomy,and the reason,diagnosis and treatment methods of progressive epidural hematoma.Methods The clinical data of 381 patients with brain traumatic injury craniotomy were retrospetively analyzed.Of 318 patients,27cases had progressive epidural hematoma during operation.Results 9 cases died because of functional failure of brain stem,the other 18 cases were all clinically cured.Conclusion If acute brain swelling and encephalocele occured during operation,the cause should be found quickly,especially when there was skull fracture or epidural hematoma on the contralateral brain,the head CT and operation should be taken immediately,and the bulging brain tissue should not be removed blindly,and the skull should not be forced to close,otherwise the prognosis maybe poor.
6.Treatment efficacy of patients with minimally conscious state and persistent vegetative state: a comparative study
Chinese Journal of Neuromedicine 2012;11(9):912-915
Objective To investigate the differences in treatment efficacy of patients with minimally conscious state (MCS) and persistent vegetative state (PVS). Methods Fifty-six continuous coma patients after heavy head injury and severe brain hemorrhage, admitted to our hospital from February 2004 to March 2011,were chosen in our study; 23 of them diagnosed as having MCS,and other 33 as having PVS by internal diagnostic criteria. All patients were treated with new integrative drug treatment and integrative systematical treatment. PVS standard scale was employed to assess the effectiveness before treatment,and 1,3 and 5 courses after treatment in the 2 groups; and the differences of efficacy in MCS patients of different ages were compared. Results The scores of PVS standard scale in the patients with MCS were significantly higher than those in the patients with PVS at 3 and 5 courses after treatment (P<0.05); the scores of PVS standard scale in the patients with MCS at 1,3 and 5 courses after treatment and patients with PVS 3 and 5 courses after treatment were obviously higher than those before treatment (P<0.05); significant difference on the efficacy was noted between the 2 groups (P<0.05); and mean rank test indicated that the treatment efficacy in patients with MCS was better than that in patients with PVS. The treatment efficacy in MCS patients of different ages was significantly different (P<0.05); patients aged 18-29 enjoyed the best treatment efficacy,while patients aged over 50 enjoyed the worst treatment efficacy. Conclusion The treatment efficacy of patients with MCS is significantly better than that of patients with PVS; integrative systematically therapy is very important for patients with MCS and it can make the majority of patients get recover.
7.Randomized, double-blind, multi-center, positive parallel control clinical trial of compound Wuzhigan capsules on anemopyretic cold.
Xiao-Mei CHEN ; Hui CAO ; Hong SUN ; Jing WEN ; Wen-Hua HUANG ; Ya-Jing HU ; Lin LIN ; Cui-Ying TANG ; Rui XU ; Hai-Tang HU
China Journal of Chinese Materia Medica 2014;39(3):531-535
Compound Wuzhigan capsules is a compound preparation composed of Wuzhigan, Shidagonglao, Gangmei, Shanzhima. A Randomized, double-blind, multi-center, positive parallel control designed to evaluate the clinical efficacy and safety of compound Wuzhigan capsules on anemopyretic cold. One hundred and twenty anemopyretic cold patients were given compound Wuzhigan capsules (test group), 2 capsules one time, three times a day, 119 patients were given compound Wuzhigan tablets (control group) ,4 tablets one time, three times a day; three days of treatment The study showed, the markedly effective rate and total effective rate respectively were 63. 3% and 80% of the test group. For the control group, the markedly effective rate and total effective rate respectively were 72. 5% and 80. 7%. The difference was not statistically significant. Compound Wuzhigan capsules can reduce the dosage, and get better patient compliance.
Adult
;
Capsules
;
Common Cold
;
complications
;
drug therapy
;
Double-Blind Method
;
Drugs, Chinese Herbal
;
adverse effects
;
therapeutic use
;
Female
;
Humans
;
Male
;
Middle Aged
;
Safety
;
Treatment Outcome
;
Young Adult
8.Biomechanical evaluation and optimal design of two parameters of dental implant with arbitrarily adjusted angles
Siyuan CHENG ; Hailin WEN ; Jingqiu SI ; Rui LIANG ; Jing NIE ; Hang WANG ; Jie LONG ; Wei TANG ; Yongtao WEI ; Weidong TIAN
Chinese Journal of Tissue Engineering Research 2014;(34):5473-5479
BACKGROUND:Oversize stress of a dental implant and its surrounding tissue is the main factor to affect the
long-term use of dental implants. So, the reasonable and precise design of implant shape is one of the important methods of prolonging the life span of dental implants.
OBJECTIVE:To make the optimal analysis and design of the diameters of connector screw and central screw of the adjustable-angle dental implant invented in the earlier stage.
METHODS: The finite element analysis model of the edentulous mandible with adjustable-angle dental implant was established by software Pro/E 5.0, Mimics 10.0 and ANSYS Workbench 14.5. The maximum equivalent
stress of dental implant-edentulous mandibular model was analyzed.
RESULTS AND CONCLUSION:The maximum equivalent stress of dental implant-edentulous mandibular model
9.Role of protein kinase C-delta in hyperthermia-induced apoptosis in tongue squamous cell carcinoma Tca8113 cells.
Wen JIANG ; Li BIAN ; Gui-qi LI ; Li-ju MA ; Rui-zhu TANG ; Yong-wen HE
West China Journal of Stomatology 2010;28(5):539-546
OBJECTIVETo study the role of protein kinase C-delta (PKC-delta) in hyperthermia-induced apoptosis in human tongue squamous cell carcinoma Tca8113 cells.
METHODSTca8113 cells were treated at 43 degrees C in a heating water bath for 0, 40, 80, 120 min after pretreatment with Rottlerin, a specific inhibitor of PKC-delta, and equal volume dimethyl sulfoxide (DMSO) for 30 min, respectively. The cells were stained by propidium iodide (PI) and Rhodamine 123 to analysis apoptotic rate and the changes of mitochondrial transmembrane potential by flow cytometry (FCM). The total proteins were extracted for Western blotting analysis of activation and proteolysis of PKC-delta, and for colorimetric assay of relative activity of Caspase-3.
RESULTSHyperthermia could induce proteolysis and activation of PKC-delta, and this was attenuated by Rottlerin. Apoptotic rate, decreasing of mitochondrial transmembrane potential and activity of Caspase-3 which being induced by hyperthermia in Tca8113 cells were inhibited by PKC-delta specific inhibitor Rottlerin. There were significantly statistical differences in apoptosis rates, mitochondrial transmembrane potential and activity of Caspase-3 between Rottlerin- and non-Rottlerin-pretreated cells after hyperthermia for 40, 80, 120 min (P < 0.01).
CONCLUSIONActivated PKC-delta may facilitate hyperthermia-induced apoptosis in Tca8113 cells, and may be one of the mechanisms of apoptosis induced by hyperthermia.
Acetophenones ; Apoptosis ; Benzopyrans ; Carcinoma, Squamous Cell ; Humans ; Protein Kinase C ; Protein Kinase C-delta
10.Experimental study of amplifying SEN virus with different probes and primers.
Ying-tang GAO ; Rui-yang CHEN ; Wen-qin SONG ; Zhi-li QI ; Li JING ; Shan-cheng QIAN
Chinese Journal of Epidemiology 2004;25(5):459-460
China
;
epidemiology
;
Circoviridae
;
genetics
;
isolation & purification
;
Circoviridae Infections
;
epidemiology
;
virology
;
DNA Virus Infections
;
epidemiology
;
transmission
;
virology
;
DNA Viruses
;
genetics
;
isolation & purification
;
DNA, Viral
;
blood
;
Hepatitis Viruses
;
genetics
;
Humans
;
Nucleic Acid Probes
;
Polymerase Chain Reaction
;
methods
;
Sequence Homology, Nucleic Acid
;
Transfusion Reaction