?Bone marrow mesenchymal stem cells ( BMSCs ) is a kind of adult stem cells mainly enriched in bone marrow, which possesses multiple differentiation potential and can differentiate into trans-germinal layer. lt is easy for BMSC to be isolated and cultured, which has the ability of repairing various tissues with efficient proliferation and expression. BMSC could be used as seed cell for the transplantation therapy of retinal disease because of its properties of immunoregulation and neurotrophin secretion. This review focuses on research progress on retinal protection of BMSCs.

Objective To investigate the results of tumor specific growth factor(TSGF) levels in in healthy people and cancer patients in Guangzhou areas .Methods The serum TSGF levels of 6 615 health check-up receivers and 287 patients with cancer in the hospital from July 2012 to June 2013 were detected .Results In health check-up receivers ,the level and positive rate of TSGF were higher in >40-50 age women .The level and positive rate of TSGF in men were increased with age .The levels of TSGF in pa-tients with lung cancer ,liver cancer and digestive system tumor were higher than that in health check-up receivers(P<0 .01) ,but there has no statistically difference between genital system cancer patients and healthy check-up receivers(P>0 .05) .Conclusion Performing TSGF test in health examination is great significance to the early diagnosis of malignant tumors .The reference value range of TSGF should be set according to different gender and age .

Objective To understand the vibrio vulnificus pollution condition in marine products in Zhuhai region and the drug resistance situation to provide the basis for further study of vibrio vulnificus and clinical medication .Methods 89 samples were col-lected from the marine products wholesale market ,pedlars′market and supermarkets in Zhuhai region and the method published by the US Food and Drug Administration (FDA) was adopted to perform the qualitative detection of vibrio vulnificus .The identifica-tion of bacteria and the drug sensitivity test were performed by using the ATB instrument .Results In 89 samples of marine prod-ucts ,vibrio vulnificus was detected in 39 samples ,the total positive rate was 43 .82% .The detection rate of oyster was as high as 60 .87% (28/46) ,followed by marine fish (39 .13% ,9/23) and marine shrimp (20 .00% ,2/10) .The drug sensitive test showed that vibrio vulnificus had the resistant strains to cephalosporins and aminoglycoside drugs .Conclusion The seafood vibrio vulnificus pollution in Zhuhai region can not be ignored .The monitoring of vibrio vulnificus in the marine products and the drug resistance a-nalysis should be strengthened for effectively preventing the outbreak of vibrio vulnificus .

Action Potentials ; drug effects ; Animals ; CA1 Region, Hippocampal ; cytology ; Drug Antagonism ; Male ; Neurons ; drug effects ; Pentylenetetrazole ; pharmacology ; Propofol ; pharmacology ; Rats ; Rats, Wistar ; Synaptic Transmission ; drug effects

Objective To study the change rule of serum brain natriurectic peptide (BNP)and uric acid(UA)in the patients with heart failure and its clinical significance.Methods Serum BNP and UA levels in 84 patients with heart failure (observation group)and 30 controls were determined and the detection results were performed the comparative analysis.Results The serum BNP and UA levels in the observation group were significantly higher than those in control group with statistical difference between them(P <0.05),the serum BNP and UA levels were in turn progressively incereased as the cardiac function deterioration from NY-HA Ⅱ to NYHA Ⅳ,the differences from each other had statistical significance (P<0.05).The correlation analysis found that ser-um BNP level had good positive correlation with the cardiac functional grading,at the same time,serum UA level was also positively correlated with the cardiac failure severity.The further analysis found that serum BNP level in the acute left heart failure was sig-nificantly higher than that in the chronic heart failure and serum UA level also had the same results(P <0.05),serum BNP level in the left heart failure relief was significantly decreased and serum UA level was also significantly decreased.Conclusion Serum BNP and UA levels are correlated with the heart failure severity.Dynamic monitoring serum BNP and UA levels in the patients with heart failure is helpful to judge the heart failure severity.

Objective To investigate the release pattern of insulin after the load of glucose in patients with polycystic ovary syndrome ( PCOS ) and normal oral glucose tolerance.Methods Sixty-three patients with PCOS were undertaken oral glucose tolerance test (OGTT) and insulin release test,while 34 women with normal menstrual cycle served as control.Results Among 63 patients with PCOS,33 cases were obese with body mass index over 25 kg/m2,including 5 with abnormal OGTT.All 30 non-obese patients with PCOS had normal OGTT.The prevalences of insulin resistance were 78.8％,16.7％,and 9.0％ in obese PCOS,non-obese PCOS,and control groups,respectively.Abnormal insulin release curve were found in 84.5％,70.0％,and 14.7％ of subjects in these 3 groups,respectively.In 58 PCOS patients with normal OGTT,the prevalence of insulin resistance was 44.8％,and 75.9％with abnormal insulin release curve. Among them,body mass index of 32 patients,whose homeostasis model assessment for insulin resistance (HOMA-IR) and fasting insulin remained in normal range,was similar to those of control group [ ( 20.52 ± 2.86 vs 20.01 ± 2.54 ) kg/m2,P＞0.05].Conclusion These findings indicate that insulin release test is useful in detecting insulin resistance.Insulin release is elevated in PCOS patients even with normal OGTT.

Objective To observe the change of six cytokines in mice infected with Echinococcus multilocularis as part of the study on immunological mechanism in the infection. Methods Mice were infected by abdominal inoculation of echinococcus protoscoleces. The change of serum level of the cytokines IL-2、IFN-?、TNF-?、IL-4、 IL-5 and IL-10 was determined by ELISA during the infection which lasted for 260 d. Results Compared with uninfected control, the levels of the cytokines all significantly increased in the 260 d. The level of IL-2 reached a peak after 80 d post-infection (p.i.), then decreased quickly after 140 d p.i., High level of TNF-? was detected after 40 d, compared to uninfected control, reached a peak at 100 d p.i., and decreased quickly after 140 d. The level of IFN-? reached a peak after 80 d p.i., and decreased slowly after 140 d p.i., The levels of IL-4, IL-5 and IL-10 remained lower before 80 d, and increased sharply after 100 days. The levels of IL-4 and IL-10 reached peaks at 100 d p.i., and that of IL-5 at 140 d p.i. Conclusion The data suggest that the induction of Th2 antibody-mediated immunity (AMI) with a parallel expansion of Th1 cell-mediated inflammatory (CMI) responses are important mechanism of the host in defending against the metacestodes. Th1 CMI plays an important role at the early stage of infection, and Th2 AMI is important in the later stage of infection.

AIM To establish a high performance liquid chromatographic method (HPLC) to determine the concentration of valsartan in human plasma. METHODS Separation was achieved on the lichrospher C 18 column. The mobile phaseconsisted of pH 3 1 phosphate buffer acetonitrile (53∶47, V/V) was used at a flow rate of 1 0 ml?min -1 . The fluorimetric excitation and emission wavelengths were set at 265 nm and 378 nm, respectively. The plasma samples were acidified with HCl, extracted with ethyl acetate. Separate the organic phase, remove the solvent and then residue was dissolved in mobile phase. RESULTS The retention time of valsartan was 12 5 min. The calibration curves were linear in the range of 5 9～ 2 360 ?g?L -1 . The precision values (RSD) of intra day and inter day were determined to be 2 83%～7 07% and 1 57%～8 41% respectively. The absolute recovery rate were 80 30%?5 13%. The method was applied to determine the peak and valley concentrations in plasma of the hypertensive treated with 80mg valsartan per day. CONCLUSION The assay was sensitive and simple. It is suitable for the study of the pharmacokinetics of valsartan.

BACKGROUND:Currently, there is a lack of efficient, non-invasive way to transplant stem cels to the target organ or tissue. Exploring a way to guide targeting transplantation of stem cels and to improve the efficiency of stem cel homing is now one of focuses in the field of stem cels research.
OBJECTIVE: To establish a simple and feasible method to chemicaly modify the cel surface using biotin-streptavidin reaction system, and to evaluate the efficiency of this method to label bone marrow mesenchymal stem cels (BMSCs) and its effects on cel biological functions.
METHODS: Passage 3 BMSCs were obtained by whole bone marrow culture method and verified by flow cytometry. Biotin, streptavidin, sulfonated biotin-N-hydroxy-succinimide were used to equip the adhesion molecule ligand, sialyated LewisX (SLeX), to the BMSCs surface. The labeling rate of BMSCs was assessed using fluorescence microscope, the vitality of BMSCs was evaluated by trypan blue staining, and the proliferation of BMSCs was evaluated by cel counting kit-8 assay. Adipogenic and osteogenic inductions were used to evaluate the effect of the method on the multi-differentiation function of BMSCs.
RESULTS AND CONCLUSION: After culture for 2 weeks, passage 3 BMSCs were obtained and confirmed by expressing CD90, CD29 and lack of CD34, CD45. Biotin, streptavidin, sulfonated biotin-N-hydroxy-succinimide were successfuly used to equip sialyated LewisX (SLeX) to the BMSCs surface and had minor effects on the vitality, proliferation, and differentiation of BMSCs. This method was simple for surface modification and had a high modification rate of 88%. The homing of BMSCs modified by this method to the target organ or tissue could be greatly enhanced. Therefore, this method potentialy could have extensive and important applications.

Objective To study the intestinal microflora changes in children with Henoch-Sch?nlein purpura (HSP). Methods The feces of 64 children with HSP (the experimental group) and 25 healthy children (the control group) were collected. 16S rRNA/DNA fluorescent quantitative polymerase chain reaction were performed to detect bacterial content of Lactobacillus, Biifdobacterium and Escherichia coli in feces. Results The amounts of Lactobacillus, and Biifdobacterium in the HSP group were lower than those of the healthy group, and the amounts of E.coli were higher, but the differences were not statistically signiifcant. The amounts of Lactobacillus and Biifdobacterium in the HSP children with gastrointestinal symptoms (abdominal pain, vomiting, e.g.) were signiifcantly lower than those in HSP children without gastrointestinal symptoms and in the healthy group. The difference was signiifcant (P<0.05). Conclusions It suggests that intestinal microlfora in children with HSP in acute phase may exist disorders, especially in the HSP children with gastrointestinal symptoms.