1.Effect of sodium quercetin-7-sulfate on enzyme activity of recombinant human phosphoinositide 3-kinase p110? catalytic subunit
Chinese Pharmacological Bulletin 1987;0(03):-
Aim To study the effect of sodium quercetin-7-sulfate (SQMS) on activity of recombinant human phosphoinositide 3-kinase (PI3-K) p110? catalytic subunit.Methods Recombinant human PI3-K p110? catalytic subunit was expressed by gene engineering.PI3-K activity was assayed by incubating recombinant PI3-K p110? with phosphatidylinositol-4,5-bisphosphate and [?-~ ~32 P]ATP; the ~ ~32 P-radiolabeled lipids were extracted with chloroform and methanol, and assessed by scintillation counter.Results SQMS showed inhibition on the recombinant p110? catalytic subunit with IC_~50 13.14 ?mol?L~-1 .Conclusions SQMS is an inhibitor of PI3-K. The recombinant PI3-K p110? catalytic subunit might be used as a molecular target for simpler target screening more effective inhibitors of PI3-K.
2.Effect of disodium quercetin-7,4′-disulfate on enzyme activity of recombinant human phosphoinositide 3-kinase p110 catalytic subunit
Chinese Traditional and Herbal Drugs 1994;0(06):-
Objective To study the effect of disodium quercetin-7,4′-disulfate (SQDS) on activity ofrecombinant human phosphoinositide 3-kinase (PI3-K) p110?catalytic subunit. Methods Recombinanthuman PI3-K p110?catalytic subunit was expressed by gene engineering. PI3-K activity was assayed byincubating recombinant PI3-K p110?with phosphatidylinositol-4,5-bisphosphate and [?-32P] ATP;the32P-radiolabeled lipids were extracted with chloroform and methanol,then assessed by scintillation counter.Results SQDS showed inhibition on the recombinant p110?catalytic subunit with IC5014.88?mol/L.Conclusion SQDS is an inhibitor of PI3-K. The recombinant PI3-K p110?catalytic subunit might be usedas a molecular target for simpler screening and development of more effective inhibitors of PI3-K.
3.Effects of sodium quercetin-7-sulfate on recombinant human protein kinase CK2 holoenzyme
Wen LIU ; Xinguang LIU ; Nianci LIANG ;
Chinese Pharmacological Bulletin 1987;0(03):-
AIM To study the effects and kinetics of sodium quercetin 7 sulphate (SQMS) on recombinant human protein kinase CK2 holoenzyme. METHODS The recombinant human CK2 holoenzyme activity was assayed by detecting incorporation of 32 P of [? 32 P]ATP into the substrate at various conditions. RESULTS SQMS was shown to strongly inhibit the holoenzyme activity of recombinant human protein kinase CK2 with an IC 50 of 1 37 ?mol?L -1 , Kinetic studies of SQMS on recombinant human CK2 showed: the inhibition was competitive with ATP and noncompetitive with casein. CONCLUSIONSQMS is an inhibitor of protein kinase CK2, and the inhibitory action of SQMS on CK2 is competitive with ATP and noncompetitive with casein.
4.Construction plan of the military infectious diseases syndromic surveillance system
Liang WEN ; Shenlong LI ; Wanyu LIU ; Wen ZOU
Military Medical Sciences 2014;(8):655-657
Combining theory and practice at home and abroad in recent years ,construction plan of the military infectious diseases syndromic surveillance system was discussed from aspects of data collection and transmission , data analysis and early warning , incident response based on the core business requirements of syndromic surveillance for infectious diseases . The study provides the demand traction for the system construction .
5.A comparative analysis on the effect of laparoscopic repair of gastric perforation with open surgery on pulmonary function
Ying YING ; Wen LIU ; Liang LI ; Jun ZHOU ; Jialiang LIU
Chinese Journal of Biochemical Pharmaceutics 2017;37(4):440-442
Objective To compare the effect of traditional open surgery and laparoscopic repair of gastric perforation on postoperative pulmonary function.Methods Fifty patients were divided into two groups according to therapeutic method.Group A underwent traditional open gastric perforation repair(n=25).Group B underwent laparoscopic repair of gastric perforation(n=25).The pulmonary functions were examined at 1 day,3 days and 7 days postoperatively.Results All patients had different degrees of postoperative pulmonary function decline.After operation,the forced vital capacity(FVC)and forced expiratory volume in 1 second(FEV1)and vital capacity(VC)in group A were significantly lower than those in preoperation(P<0.05).The patients in laparoscopic operation group decreased significantly only on the first day postoperative.The postoperative pulmonary function of patients undergoing open surgery was significantly lower than that of patients undergoing laparoscopic surgery(P<0.05).Conclusion Laparoscopic repair of gastric perforation has less damage to the lung function than traditional open gastric perforation repair.Laparoscopic surgery plays an important role in the recovery of postoperative pulmonary function.
6.Recent advances of pancreatic cancer.
Wen-ze WANG ; Zhi-yong LIANG ; Tong-hua LIU
Chinese Journal of Pathology 2007;36(1):53-55
8.Efficacy observation of percutaneous vertebroplasty combined with zoledronic acid in treatment of spinal metastatic carcinoma
Tong ZHENG ; Li ZHU ; Xianggai LIANG ; Lin LIU ; Zhizhen WEN
Chinese Journal of Postgraduates of Medicine 2015;38(2):102-104
Objective To observe the efficacy of percutaneous vertebroplasty (PVP) combined with zoledronic acid in treatment of spinal metastatic carcinoma.Methods Fifty cases of spinal metastatic carcinoma were divided into control group and treatment group by random digits table method with 25 cases each.The control group was given zoledronic acid 4 mg,intervals of 28 d intravenous drip 1 time.The treatment group was given PVP besides zoledronic acid.The visual analog scale score,activity ability score and analgesic usage score after treatment for 3 months were observed and compared.The quality of life was evaluated and the occurrence of relapse of fracture was also recorded.Results After treatment for 3 months,the visual analog scale score,activity ability score and analgesic usage score were (2.00 ± 0.17),(2.12 ± 1.38),(1.47 ± 0.10) scores in treatment group,and (7.84 ± 0.18),(3.21 ± 0.13),(3.30 ± 0.09) scores in control group,and there were significant differences between two groups (P < 0.01 or < 0.05).The occurrence of relapse of fracture was 2 cases (8%,2/25) in treatment group and 9 cases (36%,9/25) in control group,and there was significant difference between two groups (P < 0.05).Conclusions PVP combined with zoledronic acid in treatment of spinal metastatic carcinoma has many advantages such as small incision,short surgery time and rapid relief from pain.It can obviously improve the quality of life,and it is worthy of spreading in the clinic.
9.Predictive value of ABCD2 score for vertebrobasilar artery stenosis in patients with posterior circulation transient ischemic attack:a retrospective case series study
Min LI ; Yun LI ; Liang GE ; Wen SUN ; Xinfeng LIU
International Journal of Cerebrovascular Diseases 2012;(11):829-833
Objective To investigate the correlation between ABCD2 scores and vertebrobasilar artery stenosis in patients with posterior circulation transient ischemic attack (TIA).Methods The patients with posterior circulation TIA who performed cerebral angiography were enrolled in the study,and their ABCD2 scores and imaging data were analyzed.Results A total of 108 patients with posterior circulation TIA were enrolled.There were significant differences in the main trunk stenosis degree of vertebrobasilar artery with different ABCD2 scores in patients with posterior circulation TIA (P =0.005).The overall degree of stenosis increased with the increase of ABCD2 score (P =0.000).After adjustment for other vascular risk factors,ABCD2 score had significant predictive ability for whether had posterior circulation stenosis (odds ratio [OR]1.771,95% confidence interval [CI] 1.234-2.542; P =0.002) and moderate to severe stenosis (OR 2.083,95% CI 1.336-3.176; P =0.001).Its receiver operating characteristic area under the curve was 0.693(95% CI 0.586-0.800) and 0.755 (95% CI 0.661-0.849) respectively.When the ABCD2 score was ≥3,the sensitivity and specificity of predicting posterior circulation stenosis were 62.6% (95% CI 53.4%-71.7%) and 70.6% (95% CI62.0%-79.1%) respectively,and the sensitivity and specificity of predicting posterior circulation stenosis > 50% were 78.7% (95% CI 70.9%-86.4%) and 67.5% (95% CI58.7%-76.3%) respectively.Conclusions In patients with posterior circulation TIA,the stenosis degree of vertebrobasilar arterial trunk increases with the increase of ABCD2 score.In a certain extent,ABCD2 score may predict whether the main trunk of the posterior circulation has stenosis and whether has moderate to severe stenosis.
10.Biomarkers of workers exposed to manganese.
Wen-Jing ZHANG ; Zheng-Liang LIU ; Hua SHAO
Chinese Journal of Industrial Hygiene and Occupational Diseases 2010;28(12):926-928
Adult
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Biomarkers
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blood
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urine
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Female
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Humans
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Male
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Manganese
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blood
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urine
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Middle Aged
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Occupational Exposure
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adverse effects
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Young Adult