2.Analysis on Clinical Application and Significance of Skin Allergy Test of TCM Injections
Chinese Journal of Information on Traditional Chinese Medicine 2016;23(11):121-123
Type I allergic reaction is the most common and serious one in the adverse drug reactions (ADR) of TCM injections. Skin test can be used to predict the occurrence of allergic reactions, which is a simple and effective method for safe use of TCM injections. To a certain extent, it can reduce ADR of medicine and ensure the safety of clinical medication. However, the medicine instructions of TCM injections that clearly point out the need for skin test are few, and there are many problems of skin test in clinical practice. For example, lack of systematic research on skin test, no uniform standard for clinical judgment, lack of diagnostic reagents and proprietary methods of skin test. Therefore, specifications for clinical skin test of TCM injections are needed to provide guarantee for the safe use of TCM injections.
4.Research Progress in Genetic Engineering for Cellulosic Ethanol
China Biotechnology 2006;0(07):-
Lignocellulosic biomass is a promising new source of renewable biofuel that can help reduce reliance on fossil fuels.Researches have been done over the last decades.And considerable progress has been made.The abilities and pathways of natural microorganisms to produce ethanol from cellulose are different.So the researches of reconstructing and recombinating the useful genes from different strains are of great significance to improve the yield of ethanol production and reduce the cost.The characteristics and mechanisms of natural ethanologenic strains and the research progress of constructing recombinant strains was introduced and also analyzed the perspectives and challenges.
6.Evaluation for the treatment efficacy for cryptococcus neoformans meningitis by VAD1 mRNA detection with real-time fluorescent quantitative polymerase chain reaction
Ling JIANG ; Wen LI ; Qishui OU
Chinese Journal of Laboratory Medicine 2010;33(6):531-534
Objective To establish a new approach for quantitative detection of VAD1 mRNA in cryptococcus neoformans by RT-FQ-PCR, and evaluate the treatment efficacy of CNM. MethodsThe primers and TaqMan probe were designed according to the published sequence of VAD1 mRNA (GenBank),and RT-FQ-PCR method to detect VAD1 mRNA was established. Cerebrospinal fluid from 25 CNM patients and 30 controls were detected and sensitivity and specificity of the method were evaluated. VAD1 mRNA concentration in cerebrospinal fluid from both acute phase and recovery phase of 25 CNM patients were also detected and significance of CNM treatment efficacy with VAD1 mRNA analysis was evaluated. Results Correlation coefficient of standard curve was - 0. 997 9 in detection of VAD1 mRNA by RT-FQ-PCR, and the detection limit was 101 copies/μl. The intra CV of plasmid standard for high, medium and low concentrations were 0. 65% ,0. 89% and 1.23% respectively, the sensitivity of cryptococcus neoformans detection by RT-FQPCR was 96% (24/25) ,while specificity was 100% (30/30). VAD1 mRNA concentration in acute phase were significant higher than that in recovery phase (3. 042 ±0. 906 vs 2. 187 ±0. 665 ,t =4. 583 ,P <0. 01).Conclusions The established RT-FQ-PCR method for the detection of VAD1 mRNA is provided with sound sensitivity, specificity and reproducibility, which might be fit for the detection of VAD1 mRNA. The expression level of VAD1 mRNA is relevant with the treatment efficacy of CNM.
7.Detection of BK virus in urine of renal allograft recipients and its significance
Li-Ping WEN ; Jiang-Hua CHEN ;
Chinese Journal of Urology 2001;0(06):-
Objective To investigate the value of detecting BK virus(BKV)in urine of renal al- lograft recipients for the diagnosis and treatment of BKV infection.Methods Using polymerase chain re- action(PCR)method combined with DNA sequencing,61 urine samples from renal allograft recipients,30 u- fine samples from dialytic patients and 30 urine samples from healthy volunteers(controls)were detected. The results were compared among the 3 groups.Results The BKV positive rate in renal allograft recipi- ents was 36.1%,compared with 13.3% in dialytic patients(P<0.05)and 0.0% in controls(P<0.05). One renal allograft recipient who was positive for BKV developed ureteral obstruction.The BKV positive rate was 40.9%(9/22)in renal allograft recipients with rejection episode,compared with 33.3%(13/39)in the recipients without rejection episode(P>0.05);and the BKV positive rate was 36.0%(18/50)in the recip- ients with normal graft function,compared with 36.4%(4/11)in the recipients with abnormal graft function (P>0.05).Conclusions Renal allograft recipients are the high risk population who may develop BKV viruria.There is no correlation between recipients with or without rejection episode and BKV viruria,and also no correlation between recipients with normal or abnormal graft function and BKV viruria.Detection of BKV in the urine of renal allograft recipients is helpful in differential diagnosis of BKV induced ureteral obstruc- tion,and PCR method for detecting BKV DNA can be used to screen for BK virus-associated nephropathy (BKVAN).
8.Sodium nitroprusside and SOD decrease the expression of DNA-PKcs and Ku70/80 of rats cardiomyocytes
Wen JIANG ; Wei LI ; Chengbin ZHOU
Basic & Clinical Medicine 2015;(1):74-78
Objective To determine the effects of Sodium Nitroprusside ( SNP) , superoxide dismutase ( SOD) and catalase ( CAT ) on the expression of catalytic subunit of the DNA-dependent protein kinase ( DNA-PKcs ) and Ku70/80 heterodimer in cardiomycyte H9C2, as well as their expression in the myocardial tissues of SD rats . Methods H9C2 cells were co-cultured with SNP at concentreations of 10, 20 and 40 mmol/L for 6 hours, SD rats were injected with normal saline , SNP, SNP+SOD, SNP+CAT or SNP+SOD+CAT.Western blot and immuno-histochemistry assay were used to examine DNA-PKcs and Ku70/80 protein expression respectively .Results The expression of DNA-PKcs and Ku70/80 increased in H9C2 cells co-cultured with SNP when compared with control group, but they were be decreased when treated with SOD or/and CAT.The expression of DNA-Pkcs and Ku70/80 in myocardial tissues of experimental groups were higher than the control .Conclusions Radical scavengers may play a role as a protective effect for sodium nitroprusside related injury in cardiac myocytes .
9.Studying the Quality of Monitoring System of Teaching Process Quality in Sports Institutes
Tao MENG ; Wen JIANG ; Junqiang LI
Chinese Journal of Medical Education Research 2003;0(04):-
Undergraduate education is a basic stage in higher education.Undergraduate physical education is the key to improve physical education in colleges.Monitoring teaching quality is an effective measure to improve the physical teaching quality.
10.Clinical analysis of 350 pregnant women with epilepsy
Xiai YANG ; Yongli JIANG ; Lu SONG ; Qiong GAO ; Yue MA ; Wen LI ; Wen JIANG
Chinese Journal of Neurology 2017;50(2):131-136
Objective To investigate the usage of antiepileptic drugs (AEDs),epileptic seizures,pregnancy outcomes and infant feeding practices among pregnant women with epilepsy,and provide scientific evidence for eugenics.Methods The clinical characteristics of 350 pregnant women with epilepsy from Shaanxi Provincial Epilepsy & Pregnancy Register from October 2012 to July 2016 were analyzed retrospectively.Results A total of 350 patients with 376 pregnancies were included in the final analysis.Among 376 pregnancies,272 pregnancies (72.3%) were under the treatment of AEDs.Of them,246 pregnancies (90.4%) were on monotherapy,26 (9.6%) on polytherapy.Only patients during 179 pregnancies (47.6%) took folic acid in the first trimester.Seizure frequency increased in patients during 123 pregnancies (32.7%),of whom patients during 43 pregnancies (35.0%) did not use AEDs.Although other patients during 80 pregnancies (65.0%) took AEDs,regular pharmacokinetic monitoring was absent.A total of 170 pregnancies (45.2%) attained seizure-free.Seizure frequency decreased in 42 pregnancies (11.2%) and remained unchanged in 24 pregnancies (6.4%).The rates of cesarean section and natural delivery were 43.8% (158/361) and 56.2% (203/361),respectively.Totally 4.5% (17/376) pregnancies had adverse pregnancy outcome,and 1.9% (7/376) had fetal malformations.The most common congenital malformation was heart malformation,which was observed in two offspring.One offspring developed dysostosis,one developed chromosome abnormality,and another developed cerebellum deformity.In addition,one twin developed conjoined deformities.Breastfeeding rate was 52.6% (190/361).Conclusions In Shaanxi province,AEDs compliance,pharmacokinetic monitoring and usage rate of folic acid still need to be improved in pregnant women with epilepsy.Clinical trial registration Chinese Clinical Trial Registry,ChiCTR-OOC-16009277