1.The effect of intermittent moderate hypoxia on mouse nutritive metabolism.
Ling QIN ; Sai-lan WEN ; Zhi SONG
Chinese Journal of Applied Physiology 2007;23(2):177-179
Altitude
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Animals
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Blood Glucose
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metabolism
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Cholesterol
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blood
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Hypoxia
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metabolism
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Mice
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Weight Loss
2.Antiproliferation and induction differentiation of rosiglitazone in human gastric carcinoma transplanted into nude mice
Fang-Zhi CHEN ; Li-Hui ZHU ; Jian-Feng HU ; Li ZHANG ; Guo-Qing LI ; Lan WEN
Cancer Research and Clinic 2000;0(06):-
Objective To investigate the antiproliferation and induction differentiation of human gas- tric carcinoma which human gastric lower-differentiation mucinous carcinoma MGC-803 cells transplanted in- to nude mice by using rosiglitazone(ROS),and to preliminarily explore the mechanism of differentiation. Methods The mice were randomly divided into five groups:model,ATRA,ROS 25 mg?kg~(-1),ROS 50 mg/kg, ROS 100 mg/kg.After that the volumes were measured and inhibition rates were calculated.The cell cycle was detected by FCM.The protein expression level of Mucin SAC was detected by immunohistochemistry. Results The volume of tumor decreased significantly in ROS treatment groups,the differences had statistical significance compared with model group(P0.05).The xenograft tumors of ROS groups demonstrated the characteristics of differentiation.Xenograft tumor cells were arrested in G_0/G_1 phase,and the cells in S phase decreased significantly,and up-regulated Mucin SAC gene expression.Conclusion ROS could inhibit the growth of tumor,and the effect were dose-dependent with ROS.ROS could induce the differentiation of Xenograft tumor cells of gastric cancer.Its mechanism might be related to the inhibit of transition from G_1 to S phase,degrade the activity of proliferation,regulate the expres- sion of Mucin 5AC.
3.Relationship analysis of urine RBC morphology between UF-100 and phase contrast microscope
Yun-Cheng XIA ; Xu-Guang ZANG ; Zhi-Lan LI ; Xiang-Qing XU ; Wen-Ling JIANG ; LIJIANG
Journal of Chinese Physician 2001;0(09):-
Objective To study the relationship of urine RBC morphology between UF-100 urine sediment analytic instrument andphase contrast microscope.Methods The UF-100 urine sediment analytic instrument to analyze 500 urine specimens and study the relation-ship of urine RBC morphology between urine sediment analytic instrument and phase contrast microscope.Results The according perceptionof Normocytic,Microcytic and Non-classified RBC between phase contrast microscope and UF-100 urine sediment analytic instrument RBC-info are 91.4%,94.4%,83.3% respectively,the according perception between phase contrast microscope and RBC-P70Fsc are 94.9%,95.7%,94.7% respectively,and the according perception between phase contrast microscope and RBC Fsc-DW are 84.4%,86.8%,90.5% respectively,the specificity of UF-100 and phase contrast microscope in glomerular hematuria and non-glomerular hematuria are84.3%,88.1% and 83.3%,87.9% respectively.Conclusion The results show that the UF-100 urine sediment analytic instrument issimply operating,fast and high accurate,and which can instruct clinical dignose,therapy and prognosis judgement.
4.The status of occupational health of female migrant workers in traditional Chinese medicine, western medicine and bio-pharmaceutical industry in Gansu province.
Ping-Tai LIAO ; Zhen-Xia KOU ; Zhi-Lan LI ; Yu-Hong HE ; Wen-Lan YU ; An-Shou ZHO
Chinese Journal of Industrial Hygiene and Occupational Diseases 2011;29(9):657-660
OBJECTIVETo understand the status of occupational health of female migrant workers in different kinds of pharmaceutical industries in Gansu province and to provide the basis for improving occupational health condition.
METHODSOne thousand eight hundreds and one female workers from 16 enterprises were selected by cluster sampling in Gansu province and investigated by interviewing and questionnaires.
RESULTSThere were statistical significances of education level, status of residency registrations, employment relationship and occupational hazards among female workers in three types of enterprises (P < 0.05 or P < 0.01). The morbidities of skin disease in female workers for three kinds of enterprises were 4.46%, 2.53% and 3.70%, respectively. The morbidities of reproductive system disease in female workers for three kinds of enterprises were 48.57%, 36.70% and 36.11%, respectively.
CONCLUSIONThe levels of education and working conditions of female workers in the traditional Chinese medicine, western medicine plants are low. There are more severe occupational hazards in female workers of the traditional Chinese medicine plants.
Adolescent ; Adult ; Drug Industry ; Female ; Health Status ; Humans ; Middle Aged ; Occupational Exposure ; Occupational Health ; Surveys and Questionnaires ; Transients and Migrants ; Young Adult
5.Mechanism exploration on synthesis of secondary metabolites in Sorbus aucuparia cell cultures treated with yeast extract.
Lei HUANG ; Wen-Juan XIAO ; Guang YANG ; Ge MO ; Shu-Fang LIN ; Zhi-Gang WU ; Lan-Ping GUO
China Journal of Chinese Materia Medica 2014;39(11):2019-2023
Suspension cultures cell of Sorbus aucuparia (SASC) was used as materials, the changes of physiological and biochemical indexes of SASC after treatment with yeast extract (YE) were detected, and the synthetic mechanism of secondary metabolites in SASC treated with YE was preliminarily explored. The results were as follows: under the assay conditions, SASC was induced to synthesize five biphenyl compounds, and these compounds content changed differently with induction time prolonging; YE treatment inhibited cell growth, the culture medium pH was gradually reduced after treatment; water-soluble protein content showed a trend of slow decline, which was significantly increased in YE treatment group (YE group) compared with the control group (CK group), the maximum relative content was 147.76% in contrast with CK group; both YE group and CK group were extracellular Ca2+ flow influx, but the YE group flow was significantly slow than CK group. The results indicate that YE induced the cells in a stress state, which was not conducive to the growth of cells and forced the cells to synthesize biphenyl compounds against external stress; water-soluble protein may serve as intracellular enzymes involved in the synthesis of compounds regulation; Ca2+ may as signal molecule mediate cell signal transduction respond to YE stress.
Cell Culture Techniques
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instrumentation
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methods
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Culture Media
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chemistry
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metabolism
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Saccharomyces cerevisiae
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chemistry
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Secondary Metabolism
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Sorbus
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growth & development
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metabolism
6.Application research on quantitative real-time fluorescence quantitative PCR assay for Brucella
Hong-xia, ZHANG ; Wen-xue, QI ; Wen-xing, LIU ; Sen, HU ; Zhan-shui, YU ; Mei-lan, DU ; Wei-guo, XUE ; Zhi-gao, BU
Chinese Journal of Endemiology 2009;28(5):476-479
Objective To discuss a real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) wether if can be used to detect Brucella. Methods According to the BCSP31 gene sequences specific for Brucella, one pair of primers and one TaqMan probe were designed. A real-time PCR was developed with the BCSP31 fragments cloned into PMD18-T vector. The standard cure was established and the sensitivity, the species specificity and the stability of the assay were evaluated. The clinical blood specimens were detected by QT-PCR and compared with clinical diagnosis. Results The standard curve was established with the standard template and the relationship between the Ct and the DNA copy number was linear(r=0.999). The sensitivity of the real-time PCR was 5 copies/μl. The sensitivity of the common PCR was 5×102 copies/μl. The sensitivity was about 100 times higher than common PCR. Species specificity of this FQ-PCR assay evaluated using genomic DNA from 6 Bmcella strains and 5 non-Brucella strains and strong fluorescence was detected in all Brucella strains. The CV of intra-assay and inter-assay reproducibility were 0.71%,7.23%, reprectively. Twenty-four specimens from clinical brucellosis cases, 19 showed positive, the positive coincident rate was 79%(19/24). The negative results were obtained for all 31 negative control, and the negative coincident rate was 100%(31/31). Two were positive from all 30 specimens clinically suspected. Conclusions Highly specific, sensitive, repeatable and coincidental with clinic, this FQ-PCR is quite useful for rapid detection of tiny DNA of Brucella in various samples and laboratory diagnosis.
7.Cervical Metastasis from Renal Cell Carcinoma.
Wen-Xiang WANG ; Wen-Jing ZHANG ; Kai-Xuan YANG ; Xiao TANG ; Zhi-Lan PENG
Chinese Medical Journal 2016;129(8):1005-1006
8.Diagnosis and management of 57 cases with rectovaginal endometriosis.
Journal of Zhejiang University. Medical sciences 2009;38(5):531-534
OBJECTIVETo study the diagnosis and therapy of the rectovaginal endometriosis.
METHODSClinical data of 57 women with rectovaginal endometriosis admitted to the West China Second University Hospital of Sichuan University in last two years,were retrospectively reviewed.
RESULTThe average age of patients was 40.1 years. The main clinical manifestations were dysmenorrheal, changes of menorrhea and digestive stimulation. The diameter of deep endometriosis nodules was between 1-6 cm, and 77% were found to have more than one nodules. Seven of these patients had positive results in transvaginal ultrasonography; 61%(11/18) patients had elevated CA125 levels. Thirteen patients were given preoperational medical treatment, but had no effect. All patients, except one accepted laparotomic therapy of complete excision of endometriosis nodules; 23 cases underwent drug therapy after operation. No patients had recurrence in recto-vaginal septum after complete excision; only one recurred in right ovary. Patients who failed to remove the total lesion showed improvement in pain.
CONCLUSIONDiagnosis of the rectovaginal endometriosis is based on symptoms, vaginal and rectal examination, and auxiliary examination. Complete excision of endometriosis nodules is the main therapeutic method.
Adult ; Dysmenorrhea ; etiology ; Endometriosis ; classification ; diagnosis ; surgery ; Endosonography ; methods ; Female ; Humans ; Laparoscopy ; methods ; Middle Aged ; Rectal Diseases ; diagnosis ; surgery ; Retrospective Studies ; Vaginal Diseases ; diagnosis ; surgery
9.Expressions of Tau protein during the differentiation process of mesenchymal stem cells into neural cells.
Wen-Hai YAN ; Xuan-Hui XU ; Yan XU ; Xue-Fei HAN ; Lan MA ; Jian-Zhi WANG ; Ying XING
Chinese Journal of Applied Physiology 2006;22(4):419-422
AIMTo observe expressions and changes of Tau protein, pSer202 and Tau protein's contents during the differentiation process of bone-marrow mesenchymal stem cells (MSCs) into neural cells, and discuss Tau's effects on it.
METHODSEGF and bFGF were combined for the induction of 4th, 8th, and 12th-MSCs into neural cells. Expressions of Tau protein and pSer202 were tested by immunocytochemistry. ELISA assay was applied for testing Tau protein's contents during differentiation process.
RESULTSPositive rates of Tau protein in uninduced MSCs of 4th, 8th, and 12th-MSCs were under < 6%; After 14-day induction, the cellular morphologic characteristics in different passages were very similar to neurons, positive rates of Tau protein had no significant differences between passages (P > 0.05), but had differences with their uninduced groups (P < 0.05). There hadn't had expression of pSer202 in uninduced and induced groups of passages. ELISA assay indicated that there was an upward tendency in Tau protein's contents during the 14-day induction process, those in the 14th day had no significant differences between passages too (P > 0.05).
CONCLUSIONThe increase in Tau protein's expressions and its non-phosphorylated state may make for MSCs differentiating into normal neural cells and formation of neuronal processes.
Animals ; Bone Marrow Cells ; cytology ; Cell Differentiation ; Cells, Cultured ; Guinea Pigs ; Mesenchymal Stromal Cells ; cytology ; Neurons ; cytology ; tau Proteins ; metabolism
10.Determination of drug antibody ratio in an antibody-drug conjugate.
Chuan-Fei YU ; Meng LI ; Wei GUO ; Lan WANG ; Feng ZHANG ; Chun-Yu LIU ; Wen-Bo WANG ; Jun-Zhi WANG ; Kai GAO
Acta Pharmaceutica Sinica 2014;49(3):363-367
This paper reports the determination of the drug antibody ratio in an antibody-drug conjugate with two methods, i.e. LC-MS and UV/VIS, and to provide a reliable method to scientifically evaluate and effectively control the drug antibody ratio. Deglycosylated sample was analyzed with C4 column followed by MS, and the number of conjugated drugs in the antibody was determined by the molecular weight increase due to the addition of different number of drugs to the antibody, and then drug antibody ratio was calculated by weighted average of different number of drugs conjugated to the antibody. Optical density at 252 and 280 nm was measured with UV/VIS, and due to the difference of extinction coefficients between the antibody and the drug, the drug antibody ratio was calculated from linear equation with two unknowns. The drug antibody ratio was 3.21 and 3.25 respectively measured by the two methods, and the results were similar with the two methods. Our study indicated that both methods, LC-MS and UV/VIS, could be applied to the analysis of drug antibody ratio of the antibody drug conjugate.
Antibodies
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analysis
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chemistry
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Gas Chromatography-Mass Spectrometry
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methods
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Glycosylation
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Immunoconjugates
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analysis
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chemistry
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Maleimides
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analysis
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chemistry
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Molecular Weight
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Pharmaceutical Preparations
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analysis
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chemistry
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Spectrophotometry, Ultraviolet
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methods