1.Characteristics of mutations of drug tolerance gene rpoB in L-forms Mycobacterium tuberculosis of patients with coal worker's pneumoconiosis and tuberculosis in Huainan mine region.
Jun LU ; Song YE ; Wen-Li SAI
Chinese Journal of Industrial Hygiene and Occupational Diseases 2010;28(1):49-51
Aged
;
Anthracosis
;
microbiology
;
Bacterial Proteins
;
genetics
;
China
;
DNA Mutational Analysis
;
DNA, Bacterial
;
genetics
;
DNA-Directed RNA Polymerases
;
Drug Resistance, Bacterial
;
genetics
;
Humans
;
L Forms
;
genetics
;
Middle Aged
;
Mining
;
Mutation
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Mycobacterium tuberculosis
;
genetics
;
isolation & purification
;
Tuberculosis
;
microbiology
3.Paraoxonase 1 Gene Expression Is Associated with Chronic Renal Failure in the Hans of Hunan Province
Jun WEN ; Hongbo WU ; Lihua SONG
Journal of Chinese Physician 2001;0(10):-
Objective To investigate the relationship among paraoxonase-1 (PON 1) activity in serum,PON 1 Q192R polymorphism and chronic renal failure disease ( CRF ) in the hans of Hunan.Methods PON 1 activity in serum and PON 1 Q192R polymorphism of 79 patients with CRF and 90 healthy subjects as control were measured.Results PON 1 activity in serum of patients with CRF (372 6?150 6)U was significantly lower than that in control group (537 8?172 2)U(P
4.Investigation on occupational risks of welding workers in a factory.
Ye SONG ; Wen-Jun WANG ; Heng-Yan ZHANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2010;28(1):36-37
Adult
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Dust
;
Humans
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Middle Aged
;
Noise, Occupational
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Occupational Diseases
;
epidemiology
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Risk Factors
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Welding
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Workplace
;
Young Adult
5.A Progress on the Key Technologies for Discovery of Bioactive Compounds from Traditional Chinese Medicines
Huipeng SONG ; Hua YANG ; Wen GAO ; Jun CHEN ; Ping LI
World Science and Technology-Modernization of Traditional Chinese Medicine 2016;18(7):1093-1098
Traditional Chinese medicines (TCM) is one of the most important sources of new drugs.The rapid development of modern science and technology has brought new opportunities for TCM.Admittedly,new academic theory is getting into a golden period of innovation.Key technologies that enbody TCM features and adapt to modern drug-screening requirements are urgently needed.After five years' endeavor,the authors' group has made great progress in the new theories and methodologies for the discovery of bioactive compounds from TCM.In this review,a total of five key technologies:library-bioactivity-structure integration,biological and chemical fishing technology,ligand-and receptor-based virtual screening,profile-bioactivity relationship and the technology for discovering bioactive equivalent combinatorial components (BECCs),were introduced.In the text,several valuable demonstrations over the TCM-based drug discovery were provided,for uncovering the scientific basis of TCM and accelerating the process of TCM modernization.
6.Distribution of types of bacteria involved in infection after burn caused by gas outburst and analysis of drug tolerance.
Wen-jie REN ; Ming-jun WANG ; Song-yue LI
Chinese Journal of Industrial Hygiene and Occupational Diseases 2007;25(7):421-423
Adolescent
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Adult
;
Aged
;
Burns
;
etiology
;
microbiology
;
Child
;
Child, Preschool
;
Drug Resistance, Bacterial
;
Explosions
;
Female
;
Humans
;
Male
;
Middle Aged
;
Wound Infection
;
microbiology
;
Young Adult
7.Application of Jintian'ge capsule in treatment of vertebral compression fracture in senile osteoporosis patients.
Lei-jun YU ; Jun-xia WEN ; Hong-pu SONG ; Hong LIU
China Journal of Chinese Materia Medica 2015;40(5):981-984
To study the clinical application of Jintian'ge capsule in treatment of vertebral compression fracture in senile osteoporosis patients. In the study, 69 senile osteoporotic vertebral compression fracture patients hospitalized in our hospital between January 2012 and June 2014 were selected and randomly divided into the control group and the treatment group. Patients in the control group were treated by percutaneous kyphoplasty (PKP), while those in the treatment group were given Jintian'ge capsule in addition to PKP. Efforts were made to visit the patients before the surgery and in three month after that, observe and compared the vertebral height, Cobb's angle, bone density, visual analogue scale (VAS) and Oswestry disability index between patients in the two groups. According to the findings, compared with before the treatment, patients in the two groups showed significant improvements in the vertebral height, Cobb's angle, bone density, VAS and Oswestry disability index three months after the surgery (P < 0.05); During the visit three months after the treatment, compared with the control group, the treatment group showed notable increases in all parameters (P < 0.05), particularly in the vertebral height, VAS and Oswestry disability index (P < 0.05). Based on the above findings, Jintian'ge capsule is applicable in adjuvant therapy after the osteoporotic vertebral compression facture PKP surgery.
Drugs, Chinese Herbal
;
administration & dosage
;
Female
;
Fractures, Compression
;
drug therapy
;
etiology
;
Humans
;
Male
;
Middle Aged
;
Osteoporosis
;
complications
;
Osteoporotic Fractures
;
drug therapy
;
etiology
;
Spinal Fractures
;
drug therapy
;
etiology
;
Treatment Outcome
8.Pharmacokinetics of salvianolic acid A after single intravenous administration in Rhesus monkey.
Jun-ke SONG ; Wen ZHANG ; Wei-ku ZHANG ; Zhang-ying FENG ; Tao XIE ; Guan-Hua DU
Acta Pharmaceutica Sinica 2015;50(9):1142-1147
Salvianolic acid A (Sal A) is one of the most effective compounds isolated from the root of Salvia miltiorrhiza. Up to now, several studies regarding the pharmacokinetic profiles of Sal A have been reported, however there is no such study reported in monkeys, the species which is more similar to human. The aim of this study is to develop a LC-MS method for the determination of Sal A in monkey plasma and apply it to the pharmacokinetic studies of monkeys. After single intravenous administration of Sal A, the plasma concentration-time curves were observed and the main pharmacokinetic parameters were calculated. The plasma concentration at 2 min (C2 (min)) values were (28.343 ± 6.426), (45.679 ± 12.301) and (113.293 ± 24.360) mg x L(-1) for Rhesus monkeys treated with Sal A at 2.5, 5 and 10 mg x kg(-1). The area under the concentration-time curve (AUC(0-∞)) values were (3.316 ± 0.871), (5.754 ± 2.150) and (13.761 ± 2.825) μg x L(-1) x h, respectively. Furthermore, this method was improved and applied to the simultaneous determination of Sal A, Sal B and Sal C, which provided useful information for preclinical studies and clinical trials of Sal A, Sal B and Sal C.
Administration, Intravenous
;
Animals
;
Caffeic Acids
;
pharmacokinetics
;
Chromatography, Liquid
;
Drugs, Chinese Herbal
;
pharmacokinetics
;
Lactates
;
pharmacokinetics
;
Macaca mulatta
;
Mass Spectrometry
;
Plant Roots
;
chemistry
;
Salvia miltiorrhiza
;
chemistry
9.Evaluation on effect of integrative medical treatment on quality of life of rehabilitation stage in 85 patients with SARS.
Yong-jun BIAN ; Wen-sheng QI ; Qing-qiao SONG
Chinese Journal of Integrated Traditional and Western Medicine 2003;23(9):658-660
OBJECTIVETo evaluate the effect of integrative medical treatment (IMT) with serial Chinese recipes on quality of life (QOF) of rehabilitation stage in SARS patients.
METHODSEighty-five SARS patients of rehabilitation stage were enrolled in the clinical study. They were divided into the IMT group (62 patients received serial Chinese recipes and western medicine) and the control group (23 patients received western medicine alone). The serial Chinese recipes were given according to patients' syndrome, one dose per day for oral intake for 3 weeks. QOF scoring in patients was observed.
RESULTSQOF scoring in the IMT group before treatment was not significantly different from that in the control group. After 3 weeks treatment, it improved to some extent in both groups, but the improvement in the IMT group was superior to that in the control group in respect of total score and score of psychologic emotional factors.
CONCLUSIONSerial Chinese recipe could improve QOF of rehabilitation stage in SARS patients.
Adolescent ; Adult ; Aged ; Convalescence ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Male ; Middle Aged ; Ofloxacin ; therapeutic use ; Phytotherapy ; Quality of Life ; Ribavirin ; therapeutic use ; Severe Acute Respiratory Syndrome ; drug therapy ; immunology ; rehabilitation
10.Establishment and preliminary application of measuring method for three-dimensional kinematics of the hindfoot joint in vivo
Jun WEN ; Dan JIN ; Jianyi LI ; Yuzhong ZHANG ; Zao WANG ; Ke SONG ; Xiaolei GUO ; Yonghua TUO
Chinese Journal of Orthopaedics 2012;32(8):774-780
Objective To establish a new method for measuring the 3-D kinematics of hindfoot joints in vivo by using reverse engineering software together with the theory of rigid body kinematics.Methods CT images were gathered from 9 feet of 5 healthy volunteers in both the initial position (neutral position) and the terminal position (extremely inversion-adduction-dorsiflexion position).The 3-D digital modules of hindfoot joints in the initial position and terminal position were established with MIMICS 10.01 software.The data of reconstructed digital modules was inputted into the GEOMAGIC 10.0 software in STL format for twice registration,and then their relatively displacement and changes of angle in 3-D space between the two positions were calculated Results The rotation range of the tibiotalar joint was 3.89° ±2.77° in eversion,5.29°±4.47° in abduction,10.77°+5.70° in dorsiflexion,and the relative displacement was 0.78±0.59 mm towards lateral ankle,0.18±0.75 mm towards the hindfoot,(0.65±0.71) mm towards the proximal limbs;the range of subtalar joint was 16.46°±2.94° in inversion,12.77°±1.81° in adduction,6.33°±4.32° in plantarflexion,and the relative displacement was 5.50±1.45 mm towards medial ankle,1.96±1.77 mm towards forefoot,0.43±1.18 mm towards distal limbs; the range of talonavicular joint was 38.82°±5.98° in inversion,19.71°±6.33° in adduction,5.09°±6.89° in plantarflexion,and the relative displacement was (9.77±1.73) mm towards medial ankle,3.13±1.29 mm towards hindfoot,4.64±1.42 mm towards proximal limbs.Conclusion This method measuring 3-D kinematics of hindfoot joints in vivo is non-invasive and easy to operate.