In order to meet the needs of training high-quality medical persons, combining teaching reform theory with foundational courses teaching practice, we summarize some successful experience in many respects such as construction and reformation of foundational educational system, grasping subject construction goal, bringing up and cultivating scientific researchers, improving experimental conditions. All we have done reveal that the combination of medicine and engineering is the characteristic form of development.

This design is a multi-point thermometry system based on single bus digital thermometer DS1820.The single bus system of multi-point measure temperature is developed with single chip computer 89C52 and circuit units for physiological signal measurement.Obtainment method of higher resolution temperature data is given.The method makes measure temperature resolution reach to 0.1?C.It is characterized by simplicity of structure,high precision and real time,and is easy to transmit by internet network.So the system has widely applications value on clinic.

In this paper,a pulsed magnetic fields stimulation instrument is designed and realized,which provides a pulsed magnetic field with the range of maximal intensity from 0.01~2T,frequency from 0.2~100Hz and time width of pulse from 0.01~1ms.The instrument,controlled by the hand,foot or itself,can display stimulus intensity and times and output trigger signals with different waveforms to make measuring devices operate synchronistically.

PBL teaching model has been adopted as one of mainstream models in the west medical education.This article,with the characteristics of medical physics,combined with the advantage of PBL,discusses how PBL teaching mode is applied to medical physics.What we have done provides a reference for the development of PBL in medical physics.

Objective To investigate of the biological effects of the extremely low frequency electromagnetic fields (ELFEMF),and to explore the effect of rectangular ELFEMF performance parameters on rat fetal skull osteoblast. Methods Different amplitude, frequency and duty cycle of rectangular ELFEMF were used on rat fetal skull osteoblast and the proliferation and differentiation of osteoblast were examined. Results There existed action windows of amplitude and frequency with regard to the biological effect of rectangular ELFEMF. The 15 Hz, 5 mT and 15% duty cycle could significantly promote the proliferation and decrease differentiation of the cells. Conclusion The proliferation and differentiation of osteoblast could be affected by rectangular ELFEMF characterized by its amplitude, frequency and duty cycle. This study indicated that rectangular ELFEMF of 15 Hz, 5 mT and 15% duty cycle was suitable for the proliferation and differentiation of osteoblast in vitro.

Objective To develop an apparatus to measure pulse magnetic field. Methods Single chip computer and Hall-effect integrated circuit was utilized to measure the magnetic induction. The correlative hardware and software flow were given. The measuring range of apparatus was 0-50mT. Results The circuit was simple and the performance was safe and credible. The measuring precision was high. Conclusion It can be used for some kinds of magnetic induction measurement.

Background and purpose: The diagnosis of thyroid papillary carcinoma sometimes is difficult, metastasis of lymph node is the major reason of local relapse and influence prognosis.This study explores the immunohistochemical expression and their diagnostic value of CK19,HBME-1,galectin3,p27 and cyclinD1 in thyroid papillary carcinoma and the relations between p27,cyclinD1 and lymph node metastasis of papillary carcinoma.Methods: Immunohistochemical staining was used on 50 specimens of benign lesions (40 speciments of nodular goiters and 10 cases of follicular adenomas) and 50 specimens of papillary carcinomas (40 specimens of classic variants and 10 specimens of follicular variants) operated in our hospital from January of 2005 to December of 2008. Results: CK19,HBME-1 and galectin3 were expressed in cytoplasm or on the membrane, but p27 and cyclinD1 were expressed on the nucleus. The expressions of CK19,HBME-1 and galectin3 in thyroid papillary carcinoma were middle to strong positive, the positive ratios were 100%,96% and 100%, respectively;their expressions in benign lesions were negative or low positive, the positive ratios were 10%,10% and 6%, respectively;there were statistical differences between them (P<0.01). The expression of p27 in thyroid papillary carcinoma was low positive or negative, the positive ratio was 14%, but the expression of p27 in benign lesions was middle to strong positive, the ratio was 84%, there was also significant difference between them(P<0.01). The expression of CyclinD1 were similar in benign and malignant lesions of thyroid, the positive ratios were 68% and 78%, respectively, there was no statistical difference between them(P>0.05). There were significant differences(P<0.01) between the benign lesions and the papillary carcinomas in terms of the combination of CK19,HBME-1 and galectin3 expression and there were no significant differences(P>0.05) in the group of the former with underexpression of p27 between the two groups. The differences were not statistically significant (P>0.05) between the underexpression of p27 with the expression of cyclinD1 (the positive ratios in metastasis group and no metastasis group are 72.7% and 71.4%) in the lymph node metastasis. Conclusion: The combined immunohistochemical stainings of CK19,HBME-1 and galectin3 are useful on the diagnosis and differential diagnosis of thyroid papillary carcinoma, there is no predicted value of the underexpression of p27 with the expression of cyclinD1 for lymph node matastasis of thyroid papillary carcinoma.

Objective To investigate the effect of human cytomegalovirus (HCMV) on proliferation of colony forming unit T-lymphocyte (CFU -TL)and its interference methods. Methods Normal CFU - TL culture was used as blank control. Normal CFU- TL culture system plus inactivated HCMV fluid as inactivated HCMV control. The dilution of 1:10,1:100,1:1000 were added into CFU -TL colonies culture system directly as infected group. Astragalus (AMI) and ganciclovir(GCV) were added into culture system with HCMV dilution of 1:10 as experimental group. By methylcellulose semi-solid culture, different concentrations of HCMV - AD1699 affect CFU-TL and interfered by astragalus AMI, GCV. CFU - TL were surveyed. The effect of HCMV on CFU-TL proliferation was measured by MTT; HCMV-AD169 DNA in CFU-TL was found by PCR. Results 1. Compared with control group, the numbers of CFU -TL in the HCMV infection groups decreased significantly(P

Silver nanoparticles were synthesized from the extract of Fagopyri Dibotryis Rhizoma and the optimization of synthesis was studied. The absorbance of UV-visible spectroscopy was determined under the different influencing factors such as extracting time of Fagopyri Dibotryis Rhizoma powder, reation temperature of synthesis, volume of Fagopyri Dibotryis Rhizoma extract and concentration of AgNO3 to seek the optimization conditions. By means of FT-IR, TEM, DLS and XRD, the silver nanoparticles were characterized. The results showed that when the boiling time of Fagopyri Dibotryis Rhizoma powder was 5 min, resultant temperature was 25 degrees C, the volume ratio of 0.1 g x mL(-1) Fagopyri Dibotryis Rhizoma extract and 1 mmol x L(-1) AgNO3 was 1 to 10, and the reaction time was 3.5 h, the obtained silver nanoparticles had mean size about 27 nm and Zeta potential about -34.3 mV with good uniformity and dispersivity. Therefore, the green synthesis method of silver nanoparticles using extract of traditional Chinese medicine is stable and feasible.
Fagopyrum ; chemistry ; Light ; Metal Nanoparticles ; chemistry ; ultrastructure ; Microscopy, Electron, Transmission ; Particle Size ; Plant Extracts ; chemistry ; Rhizome ; chemistry ; Scattering, Radiation ; Silver ; chemistry ; Silver Nitrate ; chemistry ; Spectroscopy, Fourier Transform Infrared ; Temperature ; X-Ray Diffraction

Objective: This paper summarized the research status and progress of photodynamic therapy (PDT) and sonodynam-ic therapy (SDT), and we hope this can be a useful reference for clinics or research. Methods: In this review we expound their molecular mechanisms in detail and classify the photosensitizer and sonosensitizer, indicate their applications for the cancer treatment and summarize their new treating modes. PDT and SDT have huge potential for cancer treatment. Results: PDT and SDT have achieved a certain results, whereas there are still many problems, such as tissue oxygen content, new photosensitizer and sonosensitizer, drug dosage and mechanisms of SDT. Conclusions: PDT and SDT, which are new methods for treating cancer, have shown a good application prospect. But PDT has not been universal in clinics and SDT is still in the experiment stage.