Objective: To isolate and screen active marine microorganisms from the East China Sea and to identify the phenotypes of the isolated strains. Methods: Thirty strains of bacteria isolated from the East China Sea were selected randomly, and the active strains were screened out by the anti-Escherichia coli model. The active strains were subjected to physiological and biochemical characteristics, salt-aggregation test, and 16S rDNA sequence analysis. Neighbor-joining trees were constructed by comparing the results of 16S rDNA sequences with sequences described in the BLAST server of the National Center for Biotechnology Information (NCBI); the strains were subsequently identified to genus level. Results: Strains F81612 and F201721 were screened out with the optimum salinities of 10% and 7.5%, respectively. Their morphology and biochemical characteristics were similar to those of Bacillus sp.. 16S rDNA sequence analysis showed that sequences of F81612 had a higher similarity to those of Bacillus subtilis; F201721 was similar to Bacillus amylolique faciens. Conclusion: Two Macrolactin S-producing strains have been screened out by the anti-Escherichia coli model, and they are identified as moderate halophilic Bacillus sp.

Objective: To exploit marine microorganisms and study their secondary metabolites for new drugs. Methods: An antibacterial model was used to screen for active strains. The ethyl acetate (EtOAc) extract was separated by silica chromatography, gel filtration chromatography, and high-performance liquid chromatography. The structures of the compounds were elucidated by 1 HNMR, 13CNMR and MS technologies; Kirby-Bauer Disc Diffusion method and MTT method were employed to detect the biological activities of the separated compounds. Results: Eleven compounds were separated and identified as macrolactin A (1), 3-Hydroxyl acetyl-indole (2), 3-indolethanol (3), cyclo-(Try-Pro) (4), cyclo-(Ile-Try) (5), cyclo-(Leu-Pro) (6), cyclo-(Leu-Val) (7), cyclo-(Ile-Pro) (8), cyclo-(Phe-Val) (9), N- phenethylacetamide (10), P-hydroxy benzaldehyde (PHB) (11). Conclusion: Compound 1 shows strong inhibitory activities against Pyricularia oryzae, Escherichia coli and Staphylococcus aureus (with MIC values being 3.6, 0.45 and 6.3 μg/ml, respectively), and tumor cell lines HeLa and HepG2 (with the IC50 values being 2.0 and 1.8 μg/ml, respectively).

Asthma ; diagnosis ; Child ; Humans ; Perception

Objective: To observe the role of rmh-TNF in enhancing the anti-angiogenesis effect of cisplatin on Lewis lung carcinoma in the mice.Methods: Lewis lung carcinoma model was established in C57BL/6 mice.Sixty model mice were randomly divided into 4 groups: control group,rmh-TNF group(1500000 U/kg),cisplatin group(6.15 mg/kg), and rmh-TNF plus cisplatin group.Twelve days after implantation of cancer cells,different drugs were injected intra- tumorallv for 3d.The expression of hypoxia inducible factor-1?(HIF-1?)gene in the tumor was identified by RT-PCR. Immunohistochemistry(IHC)image analysis was performed to determine the vascular endothelial growth factor(VEGF) and kinase domain region receptor(KDR)expression and the microvessel density(MVD).Expression of matrix metallo- proteinase-2(MMP-2)was detected by flow cytometry.Results: The MVD values in the control group,the rmh-TNF group,the DDP group and the combination group were(24.76?1.28),(18.95?1.22),(19.53?1.15),(10.43?1.05),respectively,with those of the rmh-TNF and DDP groups significantly lower than that of the control group and higher than that of the combination group(all P

Emerging data indicated that HCV subverts the antiviral activity of interferon (IF); however,whether HCV core protein contributes to the process remains controversial. In the present study, we examined the effect of HCV core protein on interferon-induced antiviral gene expression and whether the effect is involved in the activation and negative regulation of the Jak/STAT signaling pathway. Our results showed that, following treatment with IFN-α, the transcription of PKR, MxA and 2'-5'OAS were down-regulated in HepG2 cells expressing the core protein. In the presence of HCV core protein,ISRE-dependent luciferase activity also decreased. Further study indicated that the core protein could inhibit the tyrosine phosphorylation of STAT1, whereas the level of STAT1 expression was unchanged.Accordingly, SOCS3, the negative regulator of the Jak/STAT pathway, was induced by HCV core protein. These results suggests that HCV core protein may interfere with the expression of some interferon-induced antiviral genes by inhibiting STAT1 phosphorylation and induction of SOCS3.

OBJECTIVETo analyze the value of intraoperative ultrasonography (IOUS) in the diagnosis and therapy of insulinoma.

METHODSFrom January 2000 to December 2007, the application of intraoperative ultrasonography used in 44 cases with insulinoma who came from department of general surgery, First Affiliated Hospital of Zhengzhou University were retrospectively analyzed. There were 19 males and 25 females in the group. Every case accepted operation and examination of IOUS.

RESULTSTumor was accurately located and its adjacent structure was also clear by IOUS in 43 cases, the other one was islet cell hyperplasia, the detection rate of tumor was 100%. The complications: one case occurred pancreatic fistula, one occurred pancreatitis, and there was no biliary fistula and hemorrhage.

CONCLUSIONSPresently, IOUS is one simple and effective method to local insulinoma, and it could improve the success rate of operation and reduce complications.

Adolescent ; Adult ; Aged ; Female ; Humans ; Insulinoma ; diagnostic imaging ; surgery ; Intraoperative Care ; Male ; Middle Aged ; Pancreatic Neoplasms ; diagnostic imaging ; surgery ; Retrospective Studies ; Treatment Outcome ; Ultrasonography ; Young Adult

OBJECTIVETo report novel mutations SEC23B gene in congenital dyserythropoietic anemia (CDA).

METHODSBy direct sequencing method, we sequenced CDAN1 and SEC23B genes in a Chinese CDA II patient, presented with chronic fatigue and dark urine, as well as his family members. Serum hepcidin was assayed by mass spectrometry.

RESULTSWe found a c.71G>A mutation and a c.74C> A mutation in the patient. In addition, a heterozygous c.55A>G mutation of HFE2 gene was found in some family members. The level of serum hepcidin of the patient was below the detection limit (<1 nmol/L).

CONCLUSIONContrary with what have been reported previously in the Europe, especially in the Italy, the gene mutations identified in this case was different and novel. The two novel mutations contribute to the diagnosis of CDAII and are the first report in East Asian CDAII patients.

Adolescent ; Adult ; Anemia, Dyserythropoietic, Congenital ; genetics ; Asian Continental Ancestry Group ; genetics ; GPI-Linked Proteins ; genetics ; Glycoproteins ; genetics ; Hepcidins ; blood ; Humans ; Male ; Middle Aged ; Mutation ; Pedigree ; Vesicular Transport Proteins ; genetics

Objective To analyze the epidemiological characteristics of Japanese encephalitis (JE) in Zhejiang Province from 2007 to 2016 for developing the measures of control and prevention. Methods The data were collected from national notifiable diseases registry system (NNDRS) and case-based JE surveillance system (JESS) from 2007 to 2016, and they were analyzed through descriptive epidemiological method and Microsoft Office Excel 2010. Results From 2007 to 2016, a total of 595 JE cases were reported in Zhejiang Province, with an average annual incidence of 0.114 per 100000 population, among which, 559 (93.95%) were laboratory confirmed. During the ten years, 20 cases died and the average annual case fatality rate was 3.36% . Cases were distributed mainly in Wenzhou, Ningbo and Taizhou Cities, which accounting for 54.79% of the total.The peak months were July, accounting for 86.89% of all the cases.And 85.71% of all the cases were in the age of 0-14 years and 47.73% were scattered children.And 22.77% of the JE cases were vaccinated but 14.01% of them did not complete the whole course.And 77.23% of the cases did not have the experience of vaccination or unknown.Conclusion The incidence of JE is decreasing. The occurrence of JE is sporadic with distinct seasonal peak and mainly concentrating in young-age children.

Objective To explore the effect of long noncoding RNA-ATB (LncRNA-ATB) on phenotypic transition and proliferation of human peritoneal mesothelial cells (HPMCs) induced by high glucose.Methods HPMCs used in experiment were divided into three groups:control group,mannitol group and hypertonic glucose group.HPMCs in control group received no treatment,and in hypertonic glucose group and mannitol group were treated with 50mmol/L D-glucose and isotonic mannitol for 72 hours,respectively.Real-time PCR was employed to detect the mRNA expression of LncRNA-ATB,E-cadherin,α-smooth muscle actin (α-SMA),connective tissue growth factor (CTGF),Cyclin D1,cyclin dependent kinase inhibitor 4 (CDK4),protein 27 (p27)and proliferating cell nuclear antigen (PCNA).Western blotting was performed to detect the proteins expression of E-cadherin,α-SMA,CTGF,Cyclin D1,CDK4,p27 and PCNA,and flow cytometry was used to test the cell cycle.Lentivirus artifice was used to up-or down-regulate the expression of LncRNA-ATB in untreated HPMCs.Real-time PCR was employed to detect the mRNA expression of E-cadherin,α-SMA and CTGF,Western blotting was performed to detect the proteins expression of E-cadherin,α-SMA and CTGF,and flow cytometry was used to test the cell cycle.Results It is revealed by Real-time PCR,Western blotting and flow cytometry that the expressions increased of LncRNA-ATB,α-SMA,CTGF,Cyclin D1,CDK4 and PCNA induced by hypertonic glucose,and decreased of E-cadherin and p27 (P＜0.05).Up-regulation of LncRNA-ATB promoted HPMCs phenotypic transition and proliferation,while down-regulation alleviated HPMCs phenotypic transition and proliferation.Conclusion Hypertonic glucose may accelerate HPMCs phenotypic transition and proliferation by up-regulating the expression of LncRNA-ATB.

OBJECTIVETo explore the Video-urodynamic characteristics of various neurogenic bladder.

METHODSA total of 1800 patients with neurogenic bladder were included in our study from December 2002 to June 2008. All patients underwent Video-urodynamic studies. Urodynamic data was collected and analyzed.

RESULTSUrodynamic study showed detrusor overactivity in 71%, of which 60% with uninhibited sphincter relaxation, and acontractile detrusor in 29% stroke patients. No upper urinary tract deterioration was found in all 42 stroke patients. Detrusor overactivity without sphincter dyssynergia was found in 70% patients with head trauma. Seven patients with Parkinson disease showed detrusor overactivity, of which 3 with delayed sphincter relaxation. Detrusor overactivity was found in 91% and detrusor sphincter dyssynergia in 83% supra-sacral spinal cord injured patients. Acontractile detrusor was found in 73% patients with conus medullaris and cauda equina injury. Overall, upper urinary tract changes were found in 12% and vesicoureteral reflux in 4% spinal cord injured patients. Urodynamic study showed acontractile detrusor in 81%, reduced compliance in 86%, upper urinary tract changes in 55% and vesicoureteral reflux in 33% patients with myelodysplasia. Most patients (92%) with protruded lumbar disc showed detrusor areflexia. Normal bladder compliance was found in 88% patients with protruded lumbar disc. Urodynamic study showed reduced bladder sensation in 81% and detrusor under-activity in 76% patients with diabetic urinary bladder disease.

CONCLUSIONSVideo-urodynamic study can provide the most detailed information about the bladder dysfunction. It is the most valuable examination before treatment of patients with neurogenic bladder.

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; Child, Preschool ; Female ; Humans ; Male ; Middle Aged ; Urinary Bladder, Neurogenic ; diagnosis ; etiology ; physiopathology ; Urodynamics