Adolescent ; Chronic Disease ; Helicobacter Infections ; complications ; drug therapy ; Humans ; Male ; Purpura, Schoenlein-Henoch ; drug therapy ; etiology ; Syndrome

AlM: To evaluate the effectiveness of Spot Vision Screener on vision screening of children without cycloplegia.METHODS:A total of 87 children (174 eyes) aged from 2~9 years old were examined with Spot Vision Screener and optometrist before cycloplegia.RESULTS: Statistical analysis demonstrated that the cylinder diopter and axis, the equivalent spherical diopter in both eyes, and the spherical diaopter in left eye had no significant change ( P>0. 05 ). However, the spherical diaopter in right eye had statistical significance. CONCLUSlON: Spot Vision Screener is a suitable instrument in vision screening of children without cycloplegia.

Hydrogen is an ideal energy due to its high conversion efficiency, recyclability and nonpolluting nature. Compared with conventional methods, biological hydrogen production process is found to be less energy intensive and more environmental friendly, and nowadays more and more attentions are being paid on its fermentative way. A survey of fermentative bio-hydrogen production process was provided, followed by its new approaches in the future development.

OBJECTIVETo determine the recognition of healthy medical students on deqi after acupuncture, reveal the qualitative and quantitative rules of deqi and understand whether these rules are the factors of the clinical application of acupuncture therapy.

METHODSThe class questionnaires were used for the investigation study on the understanding of deqi after acupuncture at Hegu (LI 4) or Zusanli (ST 36) in 86 healthy students in the clinical medicine class.

RESULTS(1) Deqi was a kind of complicated compound feelings, with many sensation qualities such as distending pain, distension and pain. (2) Deqi was a kind of mild and moderate sensations. In 10-score credit sys tem of Massachusetts General Hospital acupuncture sensation scale (MASS), the scores of distending pain (4.69 +/- 2.83), distension (4.39 +/- 2.91) and soreness and distension (3.93 +/- 2.93) were around 5 (moderate degree), the scores of stabbing pain (1.89 +/- 2.02) were around 2 (mild degree). (3) The differences in the quantitative scores were significant for stabbing pain, distending pain, distention, soreness and distention and the others before and after treatment (all P<0.05).

CONCLUSIONDeqi of acupuncture is the mild and moderate complicated sensations manifested as distension, soreness, pain and numbness. As the invasive therapy, the filiform needle puncture will bring a certain psychological impacts on the receptors. The subjective sensation is possibly the factor for the patients' selection of acupuncture treatment.

Acupuncture Points ; Acupuncture Therapy ; instrumentation ; methods ; Adult ; China ; Female ; Humans ; Male ; Needles ; Qi ; Schools, Medical ; Sensation ; Young Adult

Adult ; Aged ; Breast Neoplasms ; genetics ; metabolism ; pathology ; surgery ; Female ; Gene Amplification ; Genital Neoplasms, Male ; genetics ; metabolism ; pathology ; surgery ; Humans ; Male ; Middle Aged ; Paget Disease, Extramammary ; genetics ; metabolism ; pathology ; surgery ; Paget's Disease, Mammary ; genetics ; metabolism ; pathology ; surgery ; Penile Neoplasms ; genetics ; metabolism ; pathology ; surgery ; Receptor, ErbB-2 ; genetics ; metabolism ; Scrotum ; Vulvar Neoplasms ; genetics ; metabolism ; pathology ; surgery

Objective To estimate the venous thromboembolism (VTE) risk and prevention in critically ill patients admitted to ICU and discuss the appropriate strategy for prevention.Methods A total of 276 critically ill patients staying longer than 48 hours in ICU were enrolled for a retrospective single-center study.VTE risk assessment,methods for mechanical and pharmacological prophylaxis and demographic data were recorded.Simplified Caprini scores for VTE risk were counted in the first day and 7th day after admission to ICU,and were compared among internal medicine,surgery and trauma subgroups.Relationship between VTE risk and the clinic index was analyzed by Pearson test and Spearman test with SPSS 17.0 software.The prophylaxis strategy applied to patients without low risk of VTE was explored.Results Simplified Caprini scores were (8.71 ± 4.90) and (9.24-± 5.30) on the first day and the 7th day after admission respectively.Simplified Caprini score was significantly related to APACHE Ⅱ score (r =0.397,P =0.027).Meanwhile,simplified Caprini score in surgical and traumatic patients was higher than that in medical ill patients (14.02 ±2.01),(14.5 ± 1.29) vs.(6.55 ±3.98),P ＜0.01.The total rate of early prophylaxis measures used with mechanical prevention (13.43％) and pharmacological prophylaxis (5.22％) was only 18.28％ within 48 hours after admissioin of patients with highest riskof VTE.Even on the 7th day after admission to ICU,the total rate of prophylaxis measure employed with mechanical prevention (11.92％) and pharmacological prophylaxis (11.56％) for VTE was 25.83％.Conclusions Critically ill patients in ICU were subjected to extremely high risk of VTE.The VTE risk related closely to the severity of critically illness existed throughout the whole period of the ICU stay.Constant assessment for VTE risk and bleeding risk should be made with frequent assessment for critically ill patients.

Objective To investigate the expression of microRNA -126(miR -126)in the blood plasma in patients with diabetic retinopathy (DR)and its possible mechanism.Methods 81 patients with DR,65 patients with type 2 diabetes and 50 healthy people (control group)were selected.The expression of miR -126 in plasma was detected by real -time PCR.The serum concentration of VEGF was detected by enzyme -linked immunosorbent assay (ELISA).The correlations between variables were analyzed by Pearson correlation analysis.The value of miR -126 in predicting the occurrence of DR was analyzed by using receiver operating characteristic curve (ROC curves).Results The expressions of miR -126 in plasma in DR patients and type 2 diabetes were lower than the control group,which in DR patients was lower than the type 2 diabetes,the serum concentrations of VEGF in DR patients and type 2 diabe-tes were higher than the control group,which in DR patients was higher than the type 2 diabetes,the differences were statistically significant (all P <0.05).Pearson correlation analysis showed that the expression of miR -126 in plasma was negatively correlated with the concentration of VEGF (r =-0.352,P <0.05).ROC curve analysis showed that the area under the curve for expression level of miR -126 in predicting DR occurrence was 0.848 (95% CI:0.776 -0.919),when the expression level of miR -126 ≤0.47,the sensitivity was 83.1%,and specificity was 80.2%. Conclusion The expression of miR -126 in plasma in DR was reduced,while the serum level of VEGF was elevated.The miR -126 might through negatively regulated VEGF to regulate retinal angiogenesis.It expected to be blood indicators for early detecting DR.

Objective To explore the changes of serum levels of TGF-β1 and IL-6 in patients with diabetic retinopathy (DR) and its clinical significances.Methods 148 patients with type 2 diabetes mellitus (T2 DM) were divided into diabetic group (T2DM group,n =54),non-proliferative DR group (NDR group,n =49) and proliferative DR group(PDR group,n =45).In the same period,45 healthy subjects were selected as the control group.The clinical data were collected.The serum levels of TGF-β1 and IL-6 were detected.The clinical values of serum levels of TGF-β1 and IL-6 in predicting the progression of DR were analyzed by using the receiver operating characteristic(ROC) curve(ROC curve).Results The levels of TC in the PDR group,NDR group and T2DM group were higher than that in the control group[(5.4 ± 0.6) mmoL/L,(5.6 ± 0.8) mmol/L,(4.6 ± 0.6) mmol/L,F =15.376,P ＜ 0.001].The duration of disease,FPG,HbA1 c,TG,LDL-C in the PDR group were higher than those in the NDR group and T2DM group[(12.4 ±2.9)d,(8.2 ±2.1)d,(5.6 ± 1.7) d,F =109.167,P ＜0.001;(10.4 ±2.4) mmol/L,(9.2 ± 0.9) mmol/L,(7.6 ± 1.1) mmol/L,(4.6 ± 0.7) mmol/L,F =86.294,P ＜ 0.001;(11.1 ±1.9) ％,(9.9 ±2.1)％,(7.8 ±0.5)％,(5.5 ± 0.4)％,F =153.854,P ＜0.001;(2.6 ±0.7) mmol/L,(2.5 ±0.5) mmol/L,(1.9-± 0.4) mmol/L,(1.6 ± 0.3) mmol/L,F =38.694,P ＜ 0.001;(4.4 ± 0.8) mmol/L,(3.8 ±0.9) mmol/L,(3.1 ±0.7) mmol/L,(2.4 ±0.5) mmol/L,F =87.261,P ＜0.001],and NDR group ＞ T2DM group ＞the control group,The level of HDL-C in the PDR group were was lower than those in the NDR group,T2DM group and the control group,and NDR group ＜ T2DM group ＜ the control group,the differences were statistically significant (all P ＜ 0.05).The serum levels of TGF-β1 and IL-6 in the PDR group were higher than those in the NDR group,T2DM group and the control group [(0.92 ± 0.13) ng/mL,(0.64 ± 0.11) ng/mL,(0.47 ± 0.09) ng/mL,(0.31 ±0.07) ng/mL,F =317.850,P ＜ 0.001;(315.74 ± 52.38) pg/mL,(223.49 ± 45.17) pg/mL,(146.35 ± 41.86) pg/mL,(81.07 ± 37.54)pg/mL,F =266.606,P ＜ 0.001],which of the NDR group were higher than the T2DM group and the control group,those of T2DM group were higher than the control group,the differences were statistically significant (all P ＜ 0.05).Pearson correlation analysis showed that the serum levels of TGF-β1 and IL-6 were positively correlated with disease duration,HbAlc and LDL-C (r =0.276,0.314,0.384 and 0.304,0.335,0.416,all P ＜0.05),while serum levels of TGF-β1 and IL-6 were negatively correlated with HDL-C (r =-0.314 and -0.287,all P ＜ 0.05).The ROC curve showed that the serum levels of TGF-β1 in predicting the progression of DR,the area under the curve was 0.964(95％ CI:0.931 ～0.998),when the cutoff value of TGF-β1 was 0.78ng/mL,the sensitivity was 91.1％,and the specificity was 91.8 ％,and for the serum levels of IL-6,the area under the curve was 0.913 (95 ％ CI:0.855 ～ 0.972),when the cutoff value of IL-6 was 280.45 pg/mL,the sensitivity was 80.0％,and the specificity was 91.8％.Conclusion The serum levels of TGF-β1 and IL-6 in patients with DR are increased,and are correlated with the progression of the disease.They can be used as indicators to assess the progression of patients with disease.

Objective To explore the clinical diagnosis and treatment of cyclic vomiting syndrome in children.Methods Thirty children proved with cyclic vomiting syndrome admitted from January,1998 to January,2003 were analyzed retrospectively.Results Cyclic vomiting syndrome was most likely to occur in 3-12 years old.The male to female ratio was 3∶2.The clinical manifestations were recurrent vomiting.Twenty-one cases had inducements,while 9 cases had not inducements.It was safe and efficient that curing cyclic vomiting syndrome with cyprohetadine and amitriptyline.Conclusions If these children with cyclic vomiting syndrome are inefficient to treatment,excluding metabolizable diseases,gastrointestinal,neurological diseases,they may be diagnosed cyclic vomiting syndrome,and cured with cyprohetadine and amitriptyline.

Objective To investigate the changes of choroidal thickness in patients with retinitis pigmentosa (RP)and its effect on visual function.Methods 37 patients with RP(62 eyes)and 30 healthy volunteers(60 eyes) as the control group were selected.The subfoveal choroidal thickness(SFCT)of eyes was detected by using optical coherence tomography(OCT)technology for enhanced depth imaging(EDI).The full -field ERG was performed,and the waveform changes of ERG were recorded.According to T32 or LVC program,the visual fields of RP eyes were examined,the mean sensitivity (MS),mean defect degree (MD)and loss variance (LV)were recorded.The correlations between variables were analyzed by using Pearson correlation analysis.Results The SFCT of RP patients was (218.7 ± 62.8)μm,which of the control group was (291.5 ±54.1)μm,covariance analysis showed that there was no interaction between age and group(F =1.574,P =0.213),compared the two groups,the difference was statistically significant (t =7.347,P <0.001).The ERG latency of RP patients was longer than that of the control group,and the amplitude was lower than the control group,the differences were statistically significant(P <0.05 ).Among 62 eyes in BP patients,the light adaptation response waveforms of 26 eyes reduced,36 eyes had no waveform,there was no difference between SFCT in the waveform reduced and no waveform (P >0.05 ).The dark adaptation response waveform of 23 eyes reduced,39 eyes had no waveform,there was no difference between SFCT in the waveform reduced and no waveform(P >0.05).24 eyes accepted T32 program examined,the MD,MS,LV and SFCT were (19.6 ±6.1)dB, (10.3 ±5.2)dB,(33.9 ±14.9)dB2 and (212.9 ±69.8)μm,respectively.Pearson correlation analysis showed that the SFCT of eyes was negatively correlated with MD and LV(r =-0.502 and -0.687,all P <0.05),while was positively correlated with MS (r =0.551,P =0.005).38 eyes accepted LVC program examined,the MS and SFCT were (7.4 ±4.9)dB and (229.3 ±64.7)μm.Pearson correlation analysis showed that the SFCT of eyes was not correlated with MS (r =0.108,P =0.519).Conclusion The SFCT of RP patients is significantly reduced.But it is not related with the changes of full -field ERG waveform.The correlation with visual field examination is affected by eye condition of central vision.When central vision has less damage,SFCT can reflect central visual photographic ability.