2.The clinical significance of ADAMT13 antigen change in thrombotic thrombocytopenic purpura patients by plasma exchange treatment.
Peng YANG ; Yan-ping LI ; Hui-qin WEN
Chinese Journal of Hematology 2012;33(10):879-880
ADAM Proteins
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blood
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immunology
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ADAMTS13 Protein
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Adult
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Female
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Humans
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Male
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Middle Aged
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Plasma Exchange
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Purpura, Thrombotic Thrombocytopenic
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blood
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pathology
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therapy
3.Study on the expression of Toll-like receptor 4 in peripheral blood mononuclear cell of patients with chronic hepatitis B virus infection
Hui WANG ; Wen-Jun DU ; Lai-Ying QIN ; Liang-Wen LIU ; Shi-Jun CHEN ;
Chinese Journal of Infectious Diseases 2001;0(05):-
Objective To examine the expression of Toll-like receptor(TLR)4 protein and the transcription of TLR4 mRNA in the peripheral blood mononuclear cells(PBMC)from patients with hepatitis B virus(HBV)infection and explore the relationship between TLR4 and chronic HBV infec- tion.Methods The expression level and transcription level of TLR4 were determined by flow cytometre and reverse transcriptase polymerase chain reaction respectively in PBMC from 37 chronic hepatitis patients,28 liver cirrhosis patients,31 severe hepatitis patients and 27 healthy controls. Meanwhile,liver function,as well as blood routine test,prothrombin test activity(PTA)and HBV DNA was measured.Results The expression level and transcription level of TLR4 in patients were higher than those in healthy controls(P
4.Determination of purity of a new anti-fatty liver drug IMH by differential scanning calorimetry method and uncertainty evaluation
Hui-hui SHAO ; Kang-fan LEI ; Tong QIN ; Wen-xuan ZHANG ; Song WU ; Qing-yun YANG
Acta Pharmaceutica Sinica 2023;57(3):767-772
The purity of 4,4′-dimethoxy-5,6,5′,6′-bis (methylenedioxy)-2′-morpholine methylenebiphenyl-2-methyl formate methanesulfonate (IMH), a new drug for fatty liver treatment, was determined through differential scanning calorimetry (DSC). Analysis of two-factor non repeatability method was performed in the investigation the effects of two factors (heating rate and sample weight) on purity determination. The DSC experimental parameters were optimized as follows: heating rate was 10 ℃·min-1, temperature range was 150-300 ℃, sample weight was 2.0-4.1 mg, and N2 flow rate was 80 mL·min-1. The linear correlation coefficient (
5.The molecular mechanism of interaction of trivalent dimethylarsinous acid (DMA(III)) binding to rat hemoglobin.
Min ZHANG ; Wen-Wen WANG ; Hui-Fang JIN ; Ling-Ling BAO ; Hua NARANMANDURA ; Ying-Jie QIN ; Chun-Hui LI
Acta Pharmaceutica Sinica 2014;49(5):666-671
In our previous work, we found that trivalent dimethylarsinous acid (DMA(III)) have high affinity binding to cysteine residue 13 of rat hemoglobin. However, it is still unknown why arsenic intermediate metabolite DMA(III) has high binding affinity for Cysl3 but not for other cysteine residues 93, 140, 111 and 125. In order to better understand the molecular mechanism of DMA(III) with rat hemoglobin, we have done current study. So, SD rats were divided into control and arsenic-treated groups randomly. Arsenic species in lysate of red blood cells were analyzed by HPLC-ICP-MS, and then determined by a hybrid quadrupole TOF MS. In addition, trivalent DMA(III) binds to different cysteine residues in rat hemoglobin alpha and beta chains were also simulated by Molecular Docking. Only Cys13 in alpha chain is able to bind to DMA(III) from the experiment results. Cys13 of alpha chain in rat hemoglobin is a specific binding site for DMA(III), and we found that amino acids compose pockets structure and surround Cys13 (but not other cysteine residues), make DMA(III) much easy to bind cysteine 13. Taken together, the DMA(III) specific binding to Cys13 is related to spatial structure of Cys13.
Animals
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Arsenic
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metabolism
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Binding Sites
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Cacodylic Acid
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analogs & derivatives
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chemistry
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Chromatography, High Pressure Liquid
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Cysteine
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metabolism
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Hemoglobins
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metabolism
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Mass Spectrometry
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Peptide Fragments
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metabolism
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Rats
6.Fever and splenic lesion.
Wen-yan ZHANG ; Gan-di LI ; Xiao-jie WANG ; Hui QIN ; Wei-ping LIU
Chinese Journal of Pathology 2010;39(4):270-271
Adult
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Antineoplastic Combined Chemotherapy Protocols
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therapeutic use
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CD56 Antigen
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metabolism
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Cyclophosphamide
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therapeutic use
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Doxorubicin
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therapeutic use
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Fever of Unknown Origin
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etiology
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Humans
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Lymphoma, Extranodal NK-T-Cell
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complications
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metabolism
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pathology
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therapy
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Male
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Prednisone
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therapeutic use
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Splenectomy
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Splenic Neoplasms
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complications
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metabolism
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pathology
;
therapy
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Vincristine
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therapeutic use
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Young Adult
7.Inhibition of proliferation and influence of proto-oncogenes expression by TanshinoneⅡA in U251 cells
Hui DENG ; Huanmin LUO ; Qin GAO ; Xiaoguang LI ; Fei XIAO ; Peifen ZHANG ; Wen WENG
Chinese Journal of Pathophysiology 1989;0(06):-
AIM: To investigate the inhibitory effect of TanshinoneⅡA on U251 glioma cell line and its mechanism. METHODS: MTT was used to measure the levels of the proliferation in U251 cultured with TanshinoneⅡA at different concentrations. The effects of TanshinoneⅡA on cell cycle of U251 were observed by FCM. The expression of proto-oncogene c-myc was measured by RT-PCR. RESULTS: The proliferation of U251 was obviously inhibited by TanshinoneⅡA in a dose dependent manner. The inhibitory rate came to the peak at (54 2?0 9)%, when cultured with TanshinoneⅡA at 0 10 g/L. The outcome of FCM showed that the proportion of G 0/G 1 phase cells was increased and the proportion of S phase cells was reduced obviously, when cultured with TanshinoneⅡA at 0 10 g/L for 3 days. The RT-PCR experiment showed that the expression of proto-oncogene c-myc was notably decreased, when the dose of TanshinoneⅡA was increased. CONCLUSION: TanshinoneⅡA inhibited the proliferation of U251 and the expression of proto-oncogene c-myc.
8.MRI study of acute radiation-induced hepatic injury with super paramagnetic iron oxide enhancement in experimental model
Wen-Cai HUANG ; Jun-Kang SHEN ; Ming-Hui QIAN ; De-Qin JIN ;
Chinese Journal of Radiology 2001;0(01):-
Objective To investigate the MR imaging technique and features of acute radiation- induced hepatic injury with superparamagnetic iron oxide(SPIO)enhancement in a rabbit model.Methods On the 10th day after half-liver single 40 Gy X-ray irradiation,MR imaging before and after SPIO administration and pathologic study of 12 adult rabbits were performed.Results On the 10th post-irradiation day,TSE T_2 WI before SPIO enhancement,the irradiated liver of two rabbits showed relatively higher signal, and 1 showed slightly lower signal.With SPIO enhancement,the irradiated livers of 9 rabbits were found to be abnormal,manifesting as higher or slightly higher areas on multiple MR imaging sequences,especially the TFE T_1 WI sequence.Histological specimen with HE stain under light microscopy revealed occlusive injury of central veins(veno-occlusive disease,VOD)in each irradiated liver to some extent.Electron microscopy investigation of the irradiated liver disclosed intracellular edema,fibrin deposition,and widening of the Disse-space.Conclusion The early pathologic feature of the irradiated liver is occlusive injury of the central vein.MR imaging with SPIO enhancement is effective to valuate the early irradiation-induced liver injury.
9.Incomplete P450 17 alpha enzyme deficiency:report of six cases
Qin-Jie TIAN ; Yi-Wen ZHANG ; Zhao-Lin LU ; Gui-Hua SHA ; Hui GAO ;
Chinese Journal of Obstetrics and Gynecology 2000;0(10):-
Objectives To summarize the characteristics,differential diagnosis and management of incomplete 17 alpha-hydroxylase/17,20-1yase deficiency(17 OHD)of Chinese patients.Methods Six cases of incomplete 17 OHD from Peking Union Medical College Hospital were studied retrospectively through analyzing their clinical data,and the molecular pathogenic mechanism was discussed after literature review.Results Four cases of 46,XX incomplete 17 OHD were reported.The clinical characteristics included female phenotype,various degrees of breast development and absent or sparse axillary/pubic hair, oligomenorrhea or secondary amenorrhea,recurrent luteinized ovarian cysts,hypogonadism with persistent hyperprogesteronemia or high serum 17 alpha-hydroxyprogesterone level,with or without hypokalemic hypertension.There were also 2 cases of 46,XY incomplete 17 OHD,in which ambiguous genitalia were present besides hypokalemic hypertension.Conclusions Incomplete 17 OHD is a very rare form of congenital enzymatic deficiencies of steroid synthesis,which should be included in the differential diagnosis when there are menstrual disorders,sexual infantilism,recurrent ovarian cysts or ambiguous genitalia.Under such circumstances,hyperprogesteronemia offers a valuable clue for further investigation.
10.Rapid Detection of Down Syndrome by Gene Diagnosis Using Short Tandem Repeat Polymorphisms
hui, CHEN ; jian-hua, LIAN ; guo-ying, SONG ; hua, QI ; qin-xian, ZHANG ; xiao-wen, LI
Journal of Applied Clinical Pediatrics 2006;0(14):-
Objective To set a rapid,simple gene diagnosis method for Down syndrome.Methods Three short tandem repeats(D21S11,D21S1270,D21S1437)loci in or near Down syndrome critical region(DSCR) were analyzed and detected by polymerase chain reaction and DNA quantitative analysis in 11 core ancestry.Results There were four types by DNA quantitative analysis to different individuals at a short tandem repeats(STR) locus.In type one,a homozygote of one allelic gene was detected.In type two,a normal heterozygote of two allelic genes was found,the content or two DNA electrophoresis bands was approximately 1∶1.In type three,a Down syndrome patient of two allelic genes was discovered.The quantity of two electrophoresis bands was nearly 2∶1.In type four,the patient showed three DNA electrophoresis bands which the content was approximately 1∶1∶1.Conclusion A rapid gene diagnosis and prenatal diagnosis method for Down syndrome can be used for quantitative analysis of STR polymorphism loci.