1.The clinical significance of ADAMT13 antigen change in thrombotic thrombocytopenic purpura patients by plasma exchange treatment.
Peng YANG ; Yan-ping LI ; Hui-qin WEN
Chinese Journal of Hematology 2012;33(10):879-880
ADAM Proteins
;
blood
;
immunology
;
ADAMTS13 Protein
;
Adult
;
Female
;
Humans
;
Male
;
Middle Aged
;
Plasma Exchange
;
Purpura, Thrombotic Thrombocytopenic
;
blood
;
pathology
;
therapy
3.Study on the expression of Toll-like receptor 4 in peripheral blood mononuclear cell of patients with chronic hepatitis B virus infection
Hui WANG ; Wen-Jun DU ; Lai-Ying QIN ; Liang-Wen LIU ; Shi-Jun CHEN ;
Chinese Journal of Infectious Diseases 2001;0(05):-
Objective To examine the expression of Toll-like receptor(TLR)4 protein and the transcription of TLR4 mRNA in the peripheral blood mononuclear cells(PBMC)from patients with hepatitis B virus(HBV)infection and explore the relationship between TLR4 and chronic HBV infec- tion.Methods The expression level and transcription level of TLR4 were determined by flow cytometre and reverse transcriptase polymerase chain reaction respectively in PBMC from 37 chronic hepatitis patients,28 liver cirrhosis patients,31 severe hepatitis patients and 27 healthy controls. Meanwhile,liver function,as well as blood routine test,prothrombin test activity(PTA)and HBV DNA was measured.Results The expression level and transcription level of TLR4 in patients were higher than those in healthy controls(P
4.Determination of purity of a new anti-fatty liver drug IMH by differential scanning calorimetry method and uncertainty evaluation
Hui-hui SHAO ; Kang-fan LEI ; Tong QIN ; Wen-xuan ZHANG ; Song WU ; Qing-yun YANG
Acta Pharmaceutica Sinica 2023;58(3):767-772
The purity of 4,4′-dimethoxy-5,6,5′,6′-bis (methylenedioxy)-2′-morpholine methylenebiphenyl-2-methyl formate methanesulfonate (IMH), a new drug for fatty liver treatment, was determined through differential scanning calorimetry (DSC). Analysis of two-factor non repeatability method was performed in the investigation the effects of two factors (heating rate and sample weight) on purity determination. The DSC experimental parameters were optimized as follows: heating rate was 10 ℃·min-1, temperature range was 150-300 ℃, sample weight was 2.0-4.1 mg, and N2 flow rate was 80 mL·min-1. The linear correlation coefficient (
5.The molecular mechanism of interaction of trivalent dimethylarsinous acid (DMA(III)) binding to rat hemoglobin.
Min ZHANG ; Wen-Wen WANG ; Hui-Fang JIN ; Ling-Ling BAO ; Hua NARANMANDURA ; Ying-Jie QIN ; Chun-Hui LI
Acta Pharmaceutica Sinica 2014;49(5):666-671
In our previous work, we found that trivalent dimethylarsinous acid (DMA(III)) have high affinity binding to cysteine residue 13 of rat hemoglobin. However, it is still unknown why arsenic intermediate metabolite DMA(III) has high binding affinity for Cysl3 but not for other cysteine residues 93, 140, 111 and 125. In order to better understand the molecular mechanism of DMA(III) with rat hemoglobin, we have done current study. So, SD rats were divided into control and arsenic-treated groups randomly. Arsenic species in lysate of red blood cells were analyzed by HPLC-ICP-MS, and then determined by a hybrid quadrupole TOF MS. In addition, trivalent DMA(III) binds to different cysteine residues in rat hemoglobin alpha and beta chains were also simulated by Molecular Docking. Only Cys13 in alpha chain is able to bind to DMA(III) from the experiment results. Cys13 of alpha chain in rat hemoglobin is a specific binding site for DMA(III), and we found that amino acids compose pockets structure and surround Cys13 (but not other cysteine residues), make DMA(III) much easy to bind cysteine 13. Taken together, the DMA(III) specific binding to Cys13 is related to spatial structure of Cys13.
Animals
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Arsenic
;
metabolism
;
Binding Sites
;
Cacodylic Acid
;
analogs & derivatives
;
chemistry
;
Chromatography, High Pressure Liquid
;
Cysteine
;
metabolism
;
Hemoglobins
;
metabolism
;
Mass Spectrometry
;
Peptide Fragments
;
metabolism
;
Rats
6.Rapid Detection of Down Syndrome by Gene Diagnosis Using Short Tandem Repeat Polymorphisms
hui, CHEN ; jian-hua, LIAN ; guo-ying, SONG ; hua, QI ; qin-xian, ZHANG ; xiao-wen, LI
Journal of Applied Clinical Pediatrics 2006;0(14):-
Objective To set a rapid,simple gene diagnosis method for Down syndrome.Methods Three short tandem repeats(D21S11,D21S1270,D21S1437)loci in or near Down syndrome critical region(DSCR) were analyzed and detected by polymerase chain reaction and DNA quantitative analysis in 11 core ancestry.Results There were four types by DNA quantitative analysis to different individuals at a short tandem repeats(STR) locus.In type one,a homozygote of one allelic gene was detected.In type two,a normal heterozygote of two allelic genes was found,the content or two DNA electrophoresis bands was approximately 1∶1.In type three,a Down syndrome patient of two allelic genes was discovered.The quantity of two electrophoresis bands was nearly 2∶1.In type four,the patient showed three DNA electrophoresis bands which the content was approximately 1∶1∶1.Conclusion A rapid gene diagnosis and prenatal diagnosis method for Down syndrome can be used for quantitative analysis of STR polymorphism loci.
7.Research status of occupational methanol poisoning based on literature bibliometrics
XIONG Yan yan LUO Lei ZHU Xue qin WEN Si hui XIE Ying
China Occupational Medicine 2022;49(06):668-672
Objective
To analyze the research status and hotspot of occupational methanol poisoning at home and abroad.
Methods ,
The China National Knowledge Resource Database Wanfang Data Knowledge Service Platform and Web of Science
were used as the data sources. The relevant literatures on occupational methanol poisoning published in domestic and foreign
, Results
journals up to June 30 2021 were searched. The bibliometrics was used to analyzed the literatures. A total of 255
literatures were included in analysis. There were 187 Chinese articles and 68 English articles. Most of Chinese articles were
, ,
published from 2001 to 2005 with an average of 26.7 literatures per five years until June 2021. Among them 72 literatures
( ), ,
were published in core journals 38.5% and 176 authors from 27 provinces autonomous regions and municipality directly
,
under the central government published relevant literatures. The research contents mainly focused on the classification
, ,
poisoning mode clinical manifestations visual impairment and poisoning prevention and treatment of occupational methanol
- ,
poisoning. Most of the English literatures were published in 2016 2020 with an average of 4.9 articles per five years until June
, ( ),
2021. Among them 36 were published in SCI journals 52.9% and 57 authors from 11 countries published relevant
, , ,
literatures. The research contents mainly focused on the clinical diagnosis drug treatment intoxication mechanism visual
Conclusion
sequelae and brain injury of occupational methanol poisoning. The research on occupational methanol poisoning
, , ,
mainly focuses on clinical diagnosis clinical manifestations treatment and prognosis and pathogenesis. The focus of relevant
research at home and abroad is different.
8.Progress in Association between Genetic Correlation and Human Violent Behavior.
Hui LI ; Lei LI ; Hong-mei XU ; Zi-qin ZHAO ; Wen-bin LIU ; Huai-gu ZHOU
Journal of Forensic Medicine 2015;31(5):381-386
Human violent behavior is a complex behavior which is influenced by genetic and environmental factors. There is a trend in investigating the mechanism of violent behavior by using the genetic methods. This article reviews several candidate genes and advances in epigenetics which are associated with violent behavior. The prospects and significance of violent behavior research from the view of gene polymorphism and epigenetics are also discussed.
Aggression
;
Epigenesis, Genetic
;
Forensic Genetics
;
Humans
;
Polymorphism, Genetic
;
Violence
9.Fever and splenic lesion.
Wen-yan ZHANG ; Gan-di LI ; Xiao-jie WANG ; Hui QIN ; Wei-ping LIU
Chinese Journal of Pathology 2010;39(4):270-271
Adult
;
Antineoplastic Combined Chemotherapy Protocols
;
therapeutic use
;
CD56 Antigen
;
metabolism
;
Cyclophosphamide
;
therapeutic use
;
Doxorubicin
;
therapeutic use
;
Fever of Unknown Origin
;
etiology
;
Humans
;
Lymphoma, Extranodal NK-T-Cell
;
complications
;
metabolism
;
pathology
;
therapy
;
Male
;
Prednisone
;
therapeutic use
;
Splenectomy
;
Splenic Neoplasms
;
complications
;
metabolism
;
pathology
;
therapy
;
Vincristine
;
therapeutic use
;
Young Adult
10.Studies on Quality Standard of Radix Toddaliae Asiaticae
Lingling WEN ; Runsheng ZHENG ; Yaping XU ; Zehui QIN ; Hui XU ; Ruoting ZHAN ; Weiwen CHEN
Journal of Guangzhou University of Traditional Chinese Medicine 2015;(1):136-140,146
Objective To establish the quality standard of Radix Toddaliae Asiaticae. Methods Thin layer chromatography ( TLC) and high performance liquid chromatography ( HPLC) were used to identify and determine chloride nitidine and toddalolactone in Radix Toddaliae Asiaticae. The moisture and total ash contents were detected according to the methods recorded in appendix of Chinese Pharmacopeia (2010 edition) . Results Toddalolactone and chloride nitidine were detectable by TLC, the spots were clear and the dissociation was good. The established HPLC method was simple and accurate. The linear ranges of toddalolactone and chloride nitidine in Radix Toddaliae Asiaticae were 2.84~42.6 μg/mL and 25.6~385 μg/mL, and their recovery rates were 99.2 % ( RSD=1.12%) and 100 % ( RSD=0.71%) , respectively. The content of moisture was in the range of 75.8~98.9 mg/g and that of total ash was in the range of 12.4~33.6 mg/g. Conclusion The developed method is specific and accurate, and can provide useful reference for establishing quality standard of Radix Toddaliae Asiaticae.