Medical devices are widely used in the treatment of diseases,health care and rehabilitation process as modern science and technology products,and become an important means in the field of modern medicine. How to ensure that medical devices are used safe and effective is the common problem in production,management,use and monitoring.

Adolescent ; Chronic Disease ; Helicobacter Infections ; complications ; drug therapy ; Humans ; Male ; Purpura, Schoenlein-Henoch ; drug therapy ; etiology ; Syndrome

BACKGROUND:Compared with the normal intervertebral disk, the density of nerve fibers and number of nerve endings and neuropeptides appear to be more in the degenerated intervertebral disk. However, this phenomenon does not occur in the normal y aged disk.
OBJECTIVE:To evaluate the axonal growth and induction of a painful neuropeptide and substance P using rat dorsal root ganglion neurons and degenerated human disc cells in vitro.
METHODS:The human intervertebral discs were harvested from patients with discogenic low back pain and normal people. And extracelluar matrix extracted from human degenerative intervertebral discs was cultured with rat dorsal root ganglion neurons. The promotion of axonal growth and induction of substance P of dorsal root ganglion neurons in extracted medium were evaluated through morphology observation and enzyme-linked immunosorbent assay, respectively.
RESULTS AND CONCLUSION:Compared with the normal group, the content of nerve growth factor in the degenerative group was significantly higher and the average length of neuritis was significantly longer in the experimental group (P<0.05). After intervention with anti-nerve growth factorβ, the average length of neuritis became remarkably shorter. The percentage of substance P-immunoreactive cells was significantly higher in the degenerative group compared with the normal group (P<0.001). Nerve growth factors that highly express in the extracellular matrix from the degenerative intervertebral dick can promote neurite outgrowth of dorsal root ganglion neurons and induce release of neuropeptides related to pain transmission.

AlM: To evaluate the effectiveness of Spot Vision Screener on vision screening of children without cycloplegia.METHODS:A total of 87 children (174 eyes) aged from 2~9 years old were examined with Spot Vision Screener and optometrist before cycloplegia.RESULTS: Statistical analysis demonstrated that the cylinder diopter and axis, the equivalent spherical diopter in both eyes, and the spherical diaopter in left eye had no significant change ( P>0. 05 ). However, the spherical diaopter in right eye had statistical significance. CONCLUSlON: Spot Vision Screener is a suitable instrument in vision screening of children without cycloplegia.

Objective To evaluate the clinical value between peritoneal lavage cytology (PLC) and prognosis of gastric cancer. Methods The peritoneal lavage fluid of 60 gastric cancer patients and 12 noncancer patients were collected,different methods were used to examine the exfoliated cancer cells,and relationships between positive results and relative clinico-pathological parameters were evaluated.Results The positive rate of PLC was 31.8%,and was positively correlated with the degree of histologic differentiation of gastric cancer (P0.05). The postoperative one-year recurrence and survival rates of the positive and negative groups were significantly different(P

Adolescent ; Humans ; Leukemia, Myeloid, Acute ; etiology ; pathology ; Male ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; complications ; pathology

Adult ; Aneurysm, Dissecting ; complications ; pathology ; Anterior Wall Myocardial Infarction ; etiology ; Aortic Aneurysm, Thoracic ; complications ; Humans ; Male

OBJECTIVE:To screen the optimal technology of dog testes and penis processed with talcum powder,and to pro-vide reference for the formulation of quality control standard. METHODS:Taking the comprehensive score of crushing rate and the content of alcohol soluble extract as evaluation indexes,L9(34) orthogonal test was used to optimize processing temperature,pro-cessing time and the amount of talcum powder;validation test was conducted. According to related method in Chinese Pharmaco-poeia(2015 edition),the contents of moisture,ash,nitrogen and alcohol soluble extract were determined,and automatic amino ac-id analyzer was used to determine the content of amino acids in samples. RESULTS:The optimal processing technology were that 100 kg dog testes and penis should add into 40 kg talcum powder;the processing temperature was 350-380 ℃;processing lasted for 4 min. Results of verification test showed that the average crushing rate and the content of alcohol soluble extract were 80.2%(RSD=0.95%,n=3)and 15.7%(RSD=2.30%,n=3). In processed dog testes and penis,the contents of moisture,ash,nitro-gen and alcohol soluble extract were 5.7%,18.7%,8.3%,15.7%,respectively. Besides,there still were 16 kinds of amino ac-ids,and their total content was 42.44%. CONCLUSIONS:The optimized talcum powder processing technology of dog testes and penis is stable and practical. The content of moisture,ash,nitrogen,alcohol soluble extract and amino acid can be used as impor-tant quality standard control indexes for processed dog testes and penis.

Diagnosis, Differential ; Erythema ; diagnosis ; etiology ; pathology ; Female ; Humans ; Infant, Newborn ; Infant, Premature ; Skin ; blood supply

Objective To explore the effect of sex difference on learning and memory function and the brain tissue damage of neonatal SD rats with hypoxic-ischemic brain damage.Methods Sixty,7-day-old neonatal Sprague-Dawley rats,were divided into randomly:male control group (M group,n=15),female control group (F group,n=15),male hypoxic-ischemic brain damage group (MHIBD group,n=15) and female hypoxic-ischemic brain damage group (FHIBD group,n=15).A modified newborn rat model that had a combined hypoxic-ischemic brain damage as described by Rice-Vannucci was used.The Morris water maze was used to evaluate the ability of learning and memory.The brain MRI and transmission electron microscope(TEM) was used to evaluate the scope of brain tissue damage and the change of the synaptic ultrastructure.Results There were no differences in swimming distance,escape latency,synaptic cleft and damage brain volume between M group and F group(P＞0.05).Compare with M group and F group,MHIBD group and FHIBD group showed significant brain injury,longer escape latency ((39.38±11.40) s vs (14.86±4.42) s,(30.14±7.18)s vs (18.41±5.03) s),longer swimming distance ((15.31± 1.77) cm vs (3.68±1.50) cm,(13.18±1.79) cm vs (4.61±1.61) cm),and TEM showed the synaptic cleft was widened ((23.18± 1.36) nm vs (19.24± 1.51) nm,(21.40± 1.71) nm vs (19.87±0.94) nm),P＜0.05).MHIBD group was more seriously compromised than the FHIBD group(P＜0.05).The brain MRI showed the damage brain volume of MHIBD group were significantly larger than FHIBD group(P＜0.05).Conclusion After bypoxic ischemic brain damage,the tolerance of brain damage and / or post-injury recovery capabilities of female rats are stronger than males.