2.Detection of BK virus in urine of renal allograft recipients and its significance
Li-Ping WEN ; Jiang-Hua CHEN ;
Chinese Journal of Urology 2001;0(06):-
Objective To investigate the value of detecting BK virus(BKV)in urine of renal al- lograft recipients for the diagnosis and treatment of BKV infection.Methods Using polymerase chain re- action(PCR)method combined with DNA sequencing,61 urine samples from renal allograft recipients,30 u- fine samples from dialytic patients and 30 urine samples from healthy volunteers(controls)were detected. The results were compared among the 3 groups.Results The BKV positive rate in renal allograft recipi- ents was 36.1%,compared with 13.3% in dialytic patients(P<0.05)and 0.0% in controls(P<0.05). One renal allograft recipient who was positive for BKV developed ureteral obstruction.The BKV positive rate was 40.9%(9/22)in renal allograft recipients with rejection episode,compared with 33.3%(13/39)in the recipients without rejection episode(P>0.05);and the BKV positive rate was 36.0%(18/50)in the recip- ients with normal graft function,compared with 36.4%(4/11)in the recipients with abnormal graft function (P>0.05).Conclusions Renal allograft recipients are the high risk population who may develop BKV viruria.There is no correlation between recipients with or without rejection episode and BKV viruria,and also no correlation between recipients with normal or abnormal graft function and BKV viruria.Detection of BKV in the urine of renal allograft recipients is helpful in differential diagnosis of BKV induced ureteral obstruc- tion,and PCR method for detecting BKV DNA can be used to screen for BK virus-associated nephropathy (BKVAN).
3. Protective effect of tanshinone II a on myocardial apoptosis in rats with sepsis
Chinese Pharmaceutical Journal 2013;48(17):1459-1463
OBJECTIVE: To explore the effect of tanshinone II A (Tan II A) on the myocardial apoptosis in rats with sepsis. METHODS: The sepsis rat model was established by cecal ligation and puncture operation (CLP), and the Tan II A was given for 12 consecutive hours. Then, the following indices were measured such as the activities of Na+-K+-ATPase and SOD, the contents of TNF-α, IL-1β, calcium, MDA, apoptotic index and the protein level of Bcl-2, Bax, Fas, caspase-3, calcincurin in myocardial. RESULTS: CD Compared with sham group, CLP treatment can result in decreased myocardial activities of Na+-K+-ATPase and SOD, elevated the contents of IL-1β, calcium, MDA (P<0.05); Tan II A treatment can improve the above aberrant indices. (2) Compared with sham group, CLP treatment can elevated the myocardial apoptotic index (P<0.05), and Tan II A treatment can decrease the elevation of apoptotic index by CLP treatment (P<0.05); (3)Compared with sham group, the protein level of Bax, Fas, caspase-3 and calcincurin significantly increased, and protein level of Bcl-2 decreased (P<0.05) in CLP group, and Tan II A treatment can improve exceptional expression of the above proteins (P<0.05). CONCLUSION: Tanshinone II A shows a protective effect on the myocardial apoptosis in septic rats maybe by depressing inflammatory infiltration and oxidative stress reaction, relieving calcium overload and partly improving the exceptional expression of the proteins such as Bax, Bcl-2, Fas, caspases-3 and calcincurin.
4.Clinical analysis of 163 cases of pulmonary thromboembolism
Hua ZHANG ; Yiqiang CHEN ; Hongxia WEN ; Xiaoying ZOU ; Chen WANG
Chinese Journal of Practical Internal Medicine 2001;0(02):-
Objective To explore the role and significance of standardized scheme for diagnosis and treatment of pulmonary thromboembolism(PTE).Methods The clinical data of 163 consecutive PTE patients who were treated in our hospital from Jan.1972 to Dec.2006 were retrospectively reviewed.The patients were divided into group A and group B based on the time of application of standardized treatment and diagnosis for PTE.The clinical data of the two groups were analyzed and compared.Results The main risk factors included deep vein thrombus,operation,injury,fracture and tumors,etc.Dyspnea was the most important clinical symptoms.The incidences of dyspnea in group A and B were 92.5% and 84.6%,respectively.The diagnostic case and the ratio of final diagnosis in group B was increased compared to those in group A on an annual basis.The median time for diagnosis was shortened(P
5.Reversal of multidrug resistance by transfection of tumor necrosis factor ? and MDR1 antisense RNA into multidrug resistant breast cancer cell line
Yixin CHEN ; Shubin WANG ; Jianzeng YE ; Hua CAO ; Feiqiu WEN
Chinese Journal of Pathophysiology 1989;0(06):-
AIM:To study the reversal effects of multidrug resistance by transfecting tumor necrosis factor ?(TNF-?) cDNA and multidrug resistant 1(MDR1) gene antisense RNA into multidrug resistant breast cancer cell line MCF-7/ADR.METHODS:The recombinant vector of enhanced green fluorescent protein(EGFP) with MDR1 antisense RNA and recombinant vector of red fluorescent protein(DsRed2) with TNF-? cDNA were constructed by RT-PCR and DNA recombinant techniques.The recombinant vectors were transfected into multidrug resistant breast cancer cell line MCF-7/ADR.The cell growth curves,cell apoptosis rates,MDR1 gene expression at mRNA and P-gp levels,and the sensitivity to ADR were determined before and after the transfection.RESULTS:After the transfection,cells showed lower growth rate,higher apoptosis rate,lower MDR1 expression at mRNA and P-gp levels,and the sensitivity to ADR increased significantly.CONCLUSION:Transfection of TNF-? cDNA and MDR1 antisense RNA into multidrug resistant breast cancer cells may have good effects on reversal of multidrug resistance.
6.Baculovirus-mediated Expression of p35 Confers Resistance to Apoptosis in Human Embryo Kidney 293 cells
Jian-hua, SONG ; Chang-yong, LIANG ; Xin-wen, CHEN
Virologica Sinica 2007;22(5):389-396
Baculovirus has many advantages as vectors for gene transfer. We demonstrated that recombinant baculovirus vectors expressing p35 (Ac-CMV-p35) and eGFP (Ac-CMV-GFP) could be transduced into human kidney 293 cells efficiently. The level of transgene expression was viral dose dependent and high-level expression of the target gene could be achieved under the heterogonous promoter. MTT assay suggested that both Ac-CMV-p35 and Ac-CMV-GFP did not have cytotoxic effect on human embryo kidney 293 cells. Cell growth curve showed the Ac-CMV-p35 and Ac- CMV-GFP transduced and non-transduced cells had similar proliferation rate, so baculovirus-mediated p35expression had no adverse effect on cell proliferation. In addition, baculovirus-mediated p35 gene expression protected human embryo kidney 293 cells against apoptosis induced by various apoptosis inducers such as Actinomycin D, UV or serum-free media. These results suggested that the baculovirus vector mediated p35 gene expression was functional and it could be widely used in molecular research and even gene therapy.
7.A Progress on the Key Technologies for Discovery of Bioactive Compounds from Traditional Chinese Medicines
Huipeng SONG ; Hua YANG ; Wen GAO ; Jun CHEN ; Ping LI
World Science and Technology-Modernization of Traditional Chinese Medicine 2016;18(7):1093-1098
Traditional Chinese medicines (TCM) is one of the most important sources of new drugs.The rapid development of modern science and technology has brought new opportunities for TCM.Admittedly,new academic theory is getting into a golden period of innovation.Key technologies that enbody TCM features and adapt to modern drug-screening requirements are urgently needed.After five years' endeavor,the authors' group has made great progress in the new theories and methodologies for the discovery of bioactive compounds from TCM.In this review,a total of five key technologies:library-bioactivity-structure integration,biological and chemical fishing technology,ligand-and receptor-based virtual screening,profile-bioactivity relationship and the technology for discovering bioactive equivalent combinatorial components (BECCs),were introduced.In the text,several valuable demonstrations over the TCM-based drug discovery were provided,for uncovering the scientific basis of TCM and accelerating the process of TCM modernization.
8.Analysis of monitoring results of iodine deficiency disorders in Cangzhou city, Hebei province, from 2007 to 2009
Song-chen, WEN ; Ye-min, QI ; Hua, LIU ; Xiao, JIU
Chinese Journal of Endemiology 2011;30(2):169-172
Objective To understand the situation of iodine deficiency disorders of Cangzhou city, Hebei province, in order to provide the scientific basis for prevention and control of iodine deficiency disorders. Methods From 2007 to 2009, dynamic monitoring of iodine deficiency disorders was carried out in low iodized salt coverage places Hejian city and Xian county. Nine towns were randomly chosen in each county, 4 administrative villages were randomly selected in each town, 8 household salts were tested in each village. Every year, 1 - 3 copies of residents water samples were collected for determination of iodine; more than 100 students age 8 - 10 years old were checked the situation of goiter and urinary iodine, more than 40 students selected were measured iodine content of salt from their home; more than 50 women of childbearing age 18 - 40 years were tested of urinary iodine. Thyroid was examined with palpation; water iodine and urine iodine concentration were measured with arsenic-Ce catalytic spectrophotometry; salt iodine was detected by direct titration. Results From 2007 to 2009,median household salt iodine were 4.3,15.9,3.6,18.4,4.6,11.7 mg/kg in Hejian city and Xian county, respectively,iodized salt accounted for 34.03% (98/288), 78.82% (227/288), 29.86% (86/288), 84.72% (244/288), 37.15%(107/288),49.31% (142/288), respectively, the consuming rate of iodized salt were 27.08% (78/288),36.46%( 105/288 ), 28.13% (81/288 ), 49.31% ( 142/288 ), 37.15 % ( 107/288 ), 30.90% ( 89/288 ), respectively. Ten samples of drinking water were tested, water iodine were 5.5 - 34.4 μg/L. From 2007 to 2009, the goiter rate of students aged 8 - 10 years old was below 5%, the median of urinary iodine were 138.1,176.6,112.0 μg/L, < 100 μg/L ratio were 31.6% ( 65/206 ), 25.0% ( 52/208 ) and 44.4% (91/205 ). The median of urinary iodine of child-bearing age women were 167.3,164.2,78.1 μg/L, < 100 μg/L ratio were 28.7%(29/101 ), 19.2%(20/104) and 60.0%(60/100).Conclusions In areas with low coverage of iodized salt, iodine nutrition level are affected, the child-bearing age
9.Intestinal microflora changes in children with Henoch-Sch?nlein purpura
Wen LUO ; Hua XU ; Xiaoqing CHEN ; Huasong ZENG ; Zhuoyin LIN
Journal of Clinical Pediatrics 2014;(10):907-911
Objective To study the intestinal microflora changes in children with Henoch-Sch?nlein purpura (HSP). Methods The feces of 64 children with HSP (the experimental group) and 25 healthy children (the control group) were collected. 16S rRNA/DNA fluorescent quantitative polymerase chain reaction were performed to detect bacterial content of Lactobacillus, Biifdobacterium and Escherichia coli in feces. Results The amounts of Lactobacillus, and Biifdobacterium in the HSP group were lower than those of the healthy group, and the amounts of E.coli were higher, but the differences were not statistically signiifcant. The amounts of Lactobacillus and Biifdobacterium in the HSP children with gastrointestinal symptoms (abdominal pain, vomiting, e.g.) were signiifcantly lower than those in HSP children without gastrointestinal symptoms and in the healthy group. The difference was signiifcant (P<0.05). Conclusions It suggests that intestinal microlfora in children with HSP in acute phase may exist disorders, especially in the HSP children with gastrointestinal symptoms.
10.Impacts of the two payment packages on the hospitalization expenses of cancer patients covered by two medical insurance schemes
Wenhui MAO ; Yunyu HUANG ; Wen CHEN ; Hua LIU ; Jian LIN
Chinese Journal of Hospital Administration 2012;(11):828-832
Objective To analyze the hospitalization expenses of cancer patients covered with byitem payment and quota payment packages,and probe into the impacts on such expenses for the two payment packages.Methods Inpatient records of 600 cancer patients were sampled by random from the medical insurance databases of Zhengzhou and Fuzhou to learn their hospitalization expenses and impact factors.Results Under the by-item payment package,the expenses of urban workers’ medical insurance were found higher than those of urban residents' medical insurance,with a per capita expense of RMB 32747.70 ± 32035.01 and 23035.83 ± 22875.65 respectively.Under the quota payment package however,there were no significant differences between expenses of the two kinds of inpatients,with a per capita expense of RMB 66043.41±47562.09 and 66576.54±73417.29 respectively.Conclusion There are gaps of reimbursement level between the two basic insurance schemes,which may not disappear in a short time.Under the by-item payment package,the gap exists in the difference of perreimbursement amount; under the quota payment package,the gap is negligible between the two populations under different insurance schemes.It is recommended to make reasonable use of these different payment schemes to minimize the relative gaps in medical service accessibility caused by the difference in reimbursement level.