2.Immunophenotyping and bone marrow hematology analysis in acute myelocytic leukemia
Bin GUO ; Ning XIE ; Wen LIU ; Ying LI ; Junan LI
International Journal of Laboratory Medicine 2016;37(19):2710-2712
Objective To investigate the clinical significance in diagnosis and prognostic judgments of acute myelocytic leukemia with combined detection of morphology ,peripheral blood and immune typing of bone marrow cells .Methods Microscopic examina‐tion of bone marrow cell morphology ,automatic blood cell analyzer detection of peripheral blood parameter and flow cytometry anal‐ysis of immune markers of leukemia cells ,all these datas were analyzed statistically .Results Peripheral blood cell analysis showed that instruments may indicate the presence of abnormal or naive cells in about 85% patients ,different kinds of leukemia had signifi‐cant difference in total leukocyte count (majority higher in M1 - 2 ,M5 ;majority lower in M3) ;while anemia and thrombocytopenia were observed in most patients ,but the degree was different ;M1 - 2 and M5 can not be identified by classification of leukocyte by automatic blood cell analyzer ,CD7 ,CD10 and CD2 can cross express in myeloid leukemia ,which have a prompt effect with treatment and prognosis .Conclusion Detection of peripheral blood parameters has an important role in early screening ,differential diagnosis and prognosis judgment of leukemia ,immunological markers detection is the powerful supplement and support for leukemia diagno‐sis and typing ,sensitive markers have close contact with prognosis ,which significantly improves the effect of the diagnosis and treatment of leukemia .
3.Exploring ways of practical and innovative talents cultivation in local medical schools
Ping QI ; Jiayong ZHU ; Jiao GUO ; Hong YANG ; Bin WEN
Chinese Journal of Medical Education Research 2006;0(08):-
Based on the analysis of current condition of local medical schools and medi-cal talents demand at the grassroots level,this paper indicates that,taking students’personality development as a breakthrough point,with practice platform construction as main part,supple-mented by multi-knowledge hierarchy and scientific and cultural exposure,backed by workable policy.
4.A respective analysis on 462 cases of leukemia typing
Wen LIU ; Jun′an LI ; Bin GUO ; Ying LI ; Lihua YAO ;
International Journal of Laboratory Medicine 2015;(12):1708-1709,1712
Objective To study the relationship between FAB‐classifying standard and immunophenotype of leukemia in clinical diagnostic and treatment .Methods There were 462 cases of leukemia according to FAB‐classifying standard and 34 cases by immu‐nophenotype in our hospital from 2011 to 2013 .The results of FAB‐classifying standard and immunophenotype were statisticed and compared .Results Among the 462 cases ,there were 171 acute myeloid leukemia (AML) ,50 acute lymphoblastic leukemia (ALL) , 76 chronic leukemia ,3 rare types ,56 unclear types and 27 mismatched according the FAB‐classifying standard .The diagnostic rate was 82 .03% .34 cases of immunophenotype included 29 single phenotype ,4 double phenotypes and 1 unknown .The diagnosis rate was 97 .06% .CD13 and CD33 have high positive express in AML ;CD34 and HLA‐DR do not express in M3 ;CD7 which was known as a lymphatic antigen also was founded in AML .Conclusion The combing use of FAB‐classifying standard and immunophenotype can improve diagnostic rate ,and immunophenotype has an important role in differentiating different subtypes of leukemia .
5.Effects of intensive insulin therapy on the intestinal permeability in sepsis patients
Bin SHI ; Hong GUO ; Bin ZHANG ; Chao YIN ; Qiliang HOU ; Yongqiang CAO ; Xinzhong WEN
Parenteral & Enteral Nutrition 2010;17(1):11-12,16
Objective: To discuss the effect of intensive insulin therapy on D-lactic acid and diamine oxidase level in patients with sepsis.Methods: 48 patients was divided into control group and conventional group randomly.Content of D-lactic acid and diamine oxidase in serum was detected with absorption spectrometry before and after therapy.Result: D-lactic acid and diamine oxidase in patients decreased significantly in control group compared to that of conventional group.Conclusion: Insulin can depress interstinal permeability and ameliorate sepsis symptom resulted from bacterial translocation from intestine.
6.The compounds from n-butanol fraction of Alpinia oxyphylla.
Bin-Bin XIE ; Lei HOU ; Bao-Lin GUO ; Wen-Hua HUANG ; Jing-Guang YU
Acta Pharmaceutica Sinica 2014;49(11):1569-1573
Nine compounds were isolated from the n-butanol fraction of 95% ethanol extract of the fruit of Alpinia oxyphylla Miq. with a combination of various chromatographic approaches, including MDS resin, silica gel, reverse phase C18 and preparative HPLC. On the basis of spectroscopic data analysis, they were elucidated as (1R, 4R, 10R)-1β, 4α-dihydroxy-11, 12, 13-trinor-5, 6-eudesmen-7-one (1), 1β, 4β-dihydroxy-11, 12, 13-trinor-8, 9-eudesmen-7-one (2), oxyphyllenone A (3), oxyphyllenone B (4), rhamnocitrin (5), staphylionoside D (6), benzyl-1-O-β-D-glucopyranoside (7), 2-O-β-D-glucopyranosyl-(1S)-phenylethylene glycol (8), and (S)-1-phenylethyl-β-D-glucopyranoside (9). Among them, compound 1 is a new sesquiterpene, named as oxyphyllenone C; compounds 8 and 9 are new natural products; compounds 2 and 6 were isolated from the genus Alpinia for the first time, and compound 7 was isolated from A. oxyphylla for the first time.
1-Butanol
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Alpinia
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chemistry
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Chromatography, High Pressure Liquid
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Fruit
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chemistry
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Phytochemicals
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chemistry
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isolation & purification
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Sesquiterpenes
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chemistry
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isolation & purification
7.A preliminary study for special English teaching reform of medical laboratory
Wen LIU ; Dongsheng WANG ; Bin GUO ; Yan LEI ; Linxu ZHAO ; Fang LIN ; Yan XING ; Xiaolan GUO
Chinese Journal of Medical Education Research 2017;16(9):908-911
There are some problems with this course of medical laboratory special English, such as reference materials, teaching contents, teaching ways, the evaluation forms, etc. The department of medical laboratory of North Sichuan Medical College did exploratory reforms including 1+n teaching mode (one major teacher and several co-operational teachers in discussion section), Flipped classroom and interactive teaching, new formative assessment forms (usual performance combing final shows grade), etc. They used innovative teaching way with the purpose of establishing new teaching way , cultivating abilities of au-tonomous learning and comprehensive application.
8.Development and prospects of predicting drug polymorphs technology
Mei GUO ; Wen-xing DING ; Bo PENG ; Jin-feng LIU ; Yi-fei SU ; Bin ZHU ; Guo-bin REN
Acta Pharmaceutica Sinica 2024;58(1):76-83
Most chemical medicines have polymorphs. The difference of medicine polymorphs in physicochemical properties directly affects the stability, efficacy, and safety of solid medicine products. Polymorphs is incomparably important to pharmaceutical chemistry, manufacturing, and control. Meantime polymorphs is a key factor for the quality of high-end drug and formulations. Polymorph prediction technology can effectively guide screening of trial experiments, and reduce the risk of missing stable crystal form in the traditional experiment. Polymorph prediction technology was firstly based on theoretical calculations such as quantum mechanics and computational chemistry, and then was developed by the key technology of machine learning using the artificial intelligence. Nowadays, the popular trend is to combine the advantages of theoretical calculation and machine learning to jointly predict crystal structure. Recently, predicting medicine polymorphs has still been a challenging problem. It is expected to learn from and integrate existing technologies to predict medicine polymorphs more accurately and efficiently.
9.Establishment of blood reference ranges and the comparative study of differences with national standard in northeast Sichuan area
Bin GUO ; Ning XIE ; Wen LIU ; Jinhua ZHANG ; Zhonghai FEI ; Jun'an LI ;
International Journal of Laboratory Medicine 2015;(22):3255-3256
Objective To establish blood reference ranges and compare these ranges with national standard in northeast Sichuan area .Methods Routine blood parameters of red blood cell ,white blood cell ,hemoglobin blood platelet count were detected in 12 480 healthy adult and old persons(divided groups according to gender ,age) and 1 120 neonatal from June 2013 to June 2014 in North Si-chuan Medical College Affiliated Hospital ,and concluded their routine blood reference range ,then analyzed the difference with the national standard .Results The range of red blood cells had no statistical difference compared with national standard in different age and gender groups(P>0 .05) .The ranges of white blood cell count and hemoglobin had significant differences compared with the national standard only in the neonatal group(P<0 .05) .The range of platelet count had significant difference compared with the na-tional standard in all age and gender groups(P<0 .05) in the northeast area of sichuan .Conclusion There are significant differ-ences between the partial routine blood value in the northeast area of Sichuan and the current reference range ,and might influence the clinical judgment of abnormal results .
10.Study of the therapeutic effects of free radical scavenger edaravone on cerebral hemorrhage in rats
Ling-Lin DONG ; Fu-Qiang GUO ; You-Song YANG ; Hong-Yuan DAI ; Wen-Bin WU ;
Chinese Journal of Neurology 2001;0(01):-
Objective To explore therapeutic effects and mechanisms of radical scavenger edaravone on experimental cerebral hemorrhage.Methods Two hundred-forty male SD rats were divided randomly into four groups:control group,cerebral hemorrhage group,edaravone treatment group before operation (A) and edaravone treatment group after operation (B).Experimental cerebral hemorrhage model was made according to the method reported by Rosenberg.Water quantity contained in brain and nervous missing sign were observed,meanwhile the levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in brain tissue were measured.Results Compared with cerebral hemorrhage group,nervous missing sign and water quantity contained in brain obviously changed in edaravone treatment group (P